scholarly journals In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae

2020 ◽  
Vol 4 (2) ◽  
pp. 61
Author(s):  
Intan Nurcahya ◽  
Ratna Damayanti ◽  
Iwan Sahrial Hamid ◽  
Agus Wijaya
Keyword(s):  
Ethanol Extract ◽  
Rhipicephalus Sanguineus ◽  
Probit Analysis ◽  
Lethal Concentration ◽  
Extract Concentration ◽  
Randomized Design ◽  
Lethal Time ◽  
Completely Randomized Design ◽  
Passiflora Foetida

This study had three fold objective which were finding the effectiveness of ethanol extract of permot leaf (Passiflora foetida Linn.) towards mortality of Rhpicephalus sanguineus (R. sanguineus) larvae in vitro and also identifying lethal concentration and lethal time. Completely randomized design was used with six treatments and four replications. Five R. sanguineus were used in the treatment for all replication. The observation of mortality was done every ten minutes in five hours. Rhpicephalus sanguineus larvae were dead if there was no movement. The collected data was analyzed using ANOVA factorial and followed by Duncan’s test. The obtained data showed that ethanol extract of permot’s leaf ethanol extract was effective toward mortality of R. sanguineus larvae in vitro. The suspension of 10% extract concentration of permot leaf ethanol extract has a similar effectiveness as Noticks®. Probit analysis stated that LC50 can be reached by using the 4% concentration in two hours and 2.4% concentration in five hours. LC95 can be reached by using the 6.5% concentration in two hours and 3.93% concentration in five hours. The quickest LT50 can be reached in 0.91 hours and LT95 ­2.37 hours after treatment with 10% concentration.

scholarly journals The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro

2019 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Meta Aprilia ◽  
Poedji Hastutiek ◽  
Rochmah Kurnijasanti ◽  
Lucia Tri Suwanti ◽  
Mohammad Sukmanadi ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Probit Analysis ◽  
Boophilus Microplus ◽  
Lethal Concentration ◽  
Betel Leaf ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Research Show

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.

scholarly journals Efectiveness of Ocimum sanctum Linn. Leaves Ethanol Extract on Mortality Raillietina echinobothrida In Vitro

2019 ◽  
Vol 2 (1) ◽  
pp. 39
Author(s):  
Jessica Giovani ◽  
Setiawan Koesdarto ◽  
Mirny Lamid ◽  
Sri Agus Sudjarwo ◽  
Muchammad Yunus ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Ocimum Sanctum ◽  
The Third ◽  
Randomized Design ◽  
Raillietina Echinobothrida ◽  
Completely Randomized Design ◽  
Lc50 And Lc90

The aims of the research are to prove the anthelmintic effects of Ocimum sanctum Linn. leaves ethanol extract on Raillietina echinobothrida in vitro and to know the value of LC50 and LC90of Ocimum sanctum Linn. leaves ethanol extract. The research used completely randomized design. The research had five treatments and each treatment was done in four replications. The research used 5 Raillietina echinobothrida adult worms for each treatment and all replications. The observation and the recording were done at the first hour until the sixth hour of the research. The Raillietina echinobothrida adult worms were noted dead if there was no movement when they were disturbed by anatomic tweezer, the death of Raillietina echinobothrida were confirmed when they dipped mildly in warm water (50°C) and they showed no movements. The acquired data was analyzed using ANOVA and continued with LSD (5%). The results were Ocimum santum Linn. ethanol extract has the anthelmintic activity in 1.25%, 2.5%, 5% and 10% concentrations. The LC50 and LC90 was analyzed using probit analysis. The results of LC50 calculation were 7.8% at the third hour, 6% at the forth hour, 3.2% at the fifth hour, 2.5% at the sixth hour and the results of LC90 were 6.5% at the fifth hour, 5.2% at the sixth hour. The higher concentration of the extract, the higher anthelmintic activity worked.

scholarly journals The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro

2019 ◽  
Vol 3 (1) ◽  
pp. 19
Author(s):  
Amelia Dwita Safitri ◽  
Iwan Sahrial Hamid ◽  
Poedji Hastutiek ◽  
Setiawan Koesdarto ◽  
Rahmi Sugihartuti ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Ascaridia Galli ◽  
Vernonia Amygdalina ◽  
Randomized Design ◽  
Lethal Time ◽  
Range Test ◽  
Research Show

The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.

scholarly journals Anthelmintic Activity Ethanol Extract of Ocimum sanctum Linn. Leaves Against Ascaridia galli In Vitro

2019 ◽  
Vol 2 (1) ◽  
pp. 21
Author(s):  
Vanna Lidya Kharisma ◽  
Setiawan Koesdarto ◽  
Koesnoto Supriandono ◽  
Lucia Tri Suwanti ◽  
Sri Agus Sudjarwo ◽  
...  
Keyword(s):  
Exposure Time ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Ocimum Sanctum ◽  
Ascaridia Galli ◽  
Time Interaction ◽  
Randomized Design ◽  
Lc50 And Lc90

The aims of this research are to determine concentration, exposure time, interaction between concentration and exposure time of ethanol extract of Ocimum sanctum Linn. Leaves to cause death toward Ascaridia galli in vitro, and the value of LC50 and LC90 ethanol extract of Ocimum sanctum Linn. Leaves. Research design that has been used in the research was completely randomized design. This research used 200 samples of Ascaridia galli with length 7-11 cm without differentiating their sex. The concentration ethanol extract of Ocimum sanctum Linn. leaves were 1.25%, 2.5%, 5%, 10%. The control was using CMC-Na 0.5%. Each treatment then being replicated four times. The observation and recording of dead worm were done at 0, 3, 6, 12 and 24 hours. Ascaridia galli were declared dead if there was no movement while disturbed by anatomy tweezers and dipped in slightly warm water (50ºC). The obtained data was analyzed using Anova Factorial and continued with Duncan Multiple Range Test by SPSS for Windows 22. The result were 10% concentration and exposure time for 24 hours caused the most mortality toward Ascaridia galli. Interaction between concentration and exposure time resulted 10% concentration ethanol extract of Ocimum sanctum Linn. leaves in 24 hours caused the most mortality towards Ascaridia galli. Probit analysis was used to calculate the LC50 and LC90 of Ocimum sanctum Linn. leaves. The results were LC50 ethanol extract of Ocimum sanctum Linn. leaves at 6 hours was 14.8%, at 12 hours was 4.8% and at 24 hours was 3.0% and the LC90 at 24 hours was 9.1%.

scholarly journals Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Level ◽  
Piper Nigrum ◽  
Dilution Method ◽  
Randomized Design ◽  
Eryngium Foetidum ◽  
Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

scholarly journals POTENSI SELADA LAUT Ulva lactuca SEBAGAI ANTIFUNGI DALAM PENGENDALIAN INFEKSI SAPROLEGNIA DAN ACHLYA PADA BUDIDAYA IKAN KERLING (Tor sp)

2018 ◽  
Vol 5 (2) ◽  
pp. 183
Author(s):  
Zulfadhli Zulfadhli ◽  
Rinawati Rinawati
Keyword(s):  
Survival Rate ◽  
Research Method ◽  
Ethanol Extract ◽  
Ulva Lactuca ◽  
Test Results ◽  
Clear Zone ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Fish Survival

This research aims to determine the effectivity of seaweeds extract (Ulva lactuca) as an antifungal in the control of kerling (Tor sp) infected with saprolegnia and achlya fungal. The research was conducted in May 2018 at the MIPA Laboratory, Syiah Kuala University and the Hatchery of the Faculty of Fisheries and Marine Sciences, Teuku Umar University. The research method was experimental with a completely randomized design (RAL), 3 treatments and 3 replications. The treatment given was immersion of kerling fish seeds infected with fungi in a solution of sea lettuce extract with the concentration of treatment namely: K (control) = 0 ppm, P1=5 ppm, P2=10 ppm, dan P3=15 ppm. Phytochemical test results showed that the content of bioactive compounds contained in ethanol extract of seaweed were alkaloids, steroids and phenolic/tannins. Ethanol extract of sea lettuce can inhibit the growth of Saprolegnia and Achlya in vitro by forming a clear zone of 5.7 mm (medium). The highest percentage of fish survival rate was in the treatment (P3) concentration of 15 ppm.

scholarly journals PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

2020 ◽  
Vol 8 (1) ◽  
pp. 18
Author(s):  
Faisal Ansyarif ◽  
Mursal Ghazali ◽  
Aida Muspiah ◽  
Rina Kurnianingsih
Keyword(s):  
Culture Media ◽  
Positive Control ◽  
Negative Control ◽  
Growth Media ◽  
Extract Concentration ◽  
Tissue Culture Media ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

scholarly journals Pengaruh Uji Antibakteri Ekstrak Rimpang Jahe Terhadap Pertumbuhan Staphylococcus Aureus Dan Escherichia Coli Secara In Vitro

2020 ◽  
Vol 1 (2) ◽  
pp. 71-80
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Randomized Design ◽  
Ginger Rhizome ◽  
Completely Randomized Design

The problem of resistance is increasing along with the need for antimicrobials as an alternative in overcoming resistance problems. One solution is to utilize the content of secondary metabolite compounds in rhizome plants such as ginger. The content of compounds that are usually the most dominant in ginger such as essential oils, flavonoids, terpenoids and phenols. This study aims to determine the effect of ginger rhizome extract as an antibacterial on the growth of Staphylococcus aureus and Escherichia coli in vitro. This study was a laboratory experimental study with a completely randomized design (CRD) consisting of 7 treatments with 3 replications. The results showed that the ethanol extract of ginger rhizome had antibacterial activity against S. aureus and E. coli. At concentrations of 20%, 40% and 80% which are more effective in inhibiting the growth of these two bacteria. The higher the extract concentration level, the larger the diameter of the inhibition zone from bacterial growth. This inhibition of bacterial growth is thought to have an effect on the content of the ginger root.   Keywords: Ginger rhizome, Staphylococcus aureus, Escherichia coli, antibacterial ABSTRAK   Permasalahan tentang resistensi semakin meningkat seiring dengan kebutuhan antimikroba sebagai alternatif dalam mengatasi masalah resistensi. Salah satu solusinya yaitu dengan memanfaatkan kandungan dari senyawa metabolit sekunder pada tanaman rimpang-rimpangan seperti rimpang jahe. Kandungan senyawa yang biasanya paling dominan pada jahe seperti minyak atsiri, flavonoid, terpenoid dan fenol. Penelitian ini bertujuan untuk mengetahui pengaruh dari ekstrak rimpang jahe sebagai antbakteri terhadap pertumbuhan Staphylococcus aureus dan Escherichia coli secara in vitro. Penelitian ini termasuk penelitian eksperimental laboratorium dengan rancangan acak lengkap (RAL) yang terdiri dari 7 perlakuan dengan 3 kali ulangan. Hasil penelitian diperoleh bahwa ekstrak etanol rimpang jahe memiliki aktivitas antibakteri terhadap S. Aureus dan E. coli. Pada konsenrasi 20%, 40% dan 80% yang lebih efktif dalam menghambat pertumbuhan kedua bakteri tersebut. Semakin tinggi tingkatan konsentrasi ekstrak maka diameter zona hambat dari pertumbuhan bakteri juga semakin besar. Terhambatnya pertumbuhan bakteri ini diduga adanya pengaruh dari kandungan pada rimpang jahe.   Kata Kunci: Rimpang Jahe, Staphylococcus aureus, Escherichia coli, antibakteri

scholarly journals PEMANFAATAN DAUN KIPAHIT (Tithonia diversifolia) SEBAGAI ALTERNATIF PENGENDALIAN HAMA KEONG MAS (Pomacea canaliculata L.) PADA TANAMAN PADI.

2019 ◽  
Vol 2 (1) ◽  
pp. 399-412
Author(s):  
Tri Bagus Wicaksono ◽  
Saifuddin Hasjim ◽  
Nanang Tri Haryadi
Keyword(s):  
Leaf Extract ◽  
Probit Analysis ◽  
Crop Damage ◽  
Pomacea Canaliculata ◽  
Tithonia Diversifolia ◽  
Crop Failure ◽  
Randomized Design ◽  
Lethal Time ◽  
Completely Randomized Design ◽  
Two Phases

Golden snail (Pomacea canaliculata L.) is one of the main pests that attack young paddy plants. Golden snail pest attack on paddy plants has the potential to reduce yields and even crop failure. Kipahit plants (T. diversifolia) have the potential as bio molluscides because they contain toxic compounds against golden snails. The purpose of this research was to study the effect of bio molluscicide concentration of kipahit leaf extract (T.diversifolia) on mortality of golden snails at two phases of age and damage intensity of paddy  plants. The. The research was conducted in December 2017 until May 2018. The research was carried out in Lampeji Village, Mumbulsari District, Jember Regency. The researh used a Completely Randomized Design (CRD) with 6 concentrations of kipahit leaf extract, tested on 2 different age phases of golden snail and 3 times repeated. The variables observed were golden snail mortality, crop damage intensity (DI), Lethal Concentration 50 (LC50) and Lethal Time 50 (LT50). The data obtained were then analyzed by probit analysis and Duncan test with a confidence level of 95%. The results showed that the concentration of 60 g/L was the most efficient and effective concentration in controlling golden snails at various age phases as well as suppressing the intensity of damage to paddy plants. Keywords: golden snail, kipahit leaves (T. diversifolia), molluscicide, mortality

scholarly journals The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro

2021 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
Dwi Retno Widyawati ◽  
Iwan Sahrial Hamid ◽  
Moh. Sukmanadi ◽  
Sri Agus Sudjarwo ◽  
Muhammad Hambal
Keyword(s):  
Leaf Extract ◽  
Probit Analysis ◽  
Boophilus Microplus ◽  
Lethal Concentration ◽  
Ageratum Conyzoides ◽  
The Third ◽  
Lethal Time ◽  
One Way Anova

The purpose of this research is to find the effectiveness of bandotan’s (Ageratum conyzoides L.) leaf extract on mortality of Boophilus microplus in vitro and also to get of lethal concentration and lethal time. This research used 125 Boophilus microplus. This research used five treatments, namely neguvon suspensionon (K+), ethanol 1% (K-), 1% concentration (P1), 2% concentration (P2) and 3% concentration (P3). The observation of mortality was done every six hours. Boophilus microplus dies when there is no movement. The data were analyzed using one-way ANOVA and followed by Duncan's Multiple Distance Test. The result is said to be significant if p<0.05. Probit analysis of LC50 is 1.63% at the first hour, 0.85% at the second hour, 0.54% at the third hour, 0.34% at the fourth hour, 0.04% at the fifth hour and 0.04% at the sixth hour, LC90 reached by using 5.6%, 2.9%, 1.8%, 1.2%, 0.16 and 0.16% concentration in 1, 2, 3, 4, 5 and 6 hours, respectively. The LT50 of ethanol 1% (K-) was at 10.142 hours, neguvon suspension on (K+) was at 0.747 hours, 1% concentration (P1) was at 2.184 hours, 2% concentration (P2) was at 1.579 hours, 3% concentration (P3) was at 0.923 hours and LT90 was at 23.734, 1.749, 5.110, 3.695 and 2.159 hours. The conclusion of this study was bandotan’s (Ageratum conyzoides L.) leaf extract was effective as an acaricide based on the mortality of Boophilus microplus.

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