Comparison of serum soluble edoglin (sEng) level in eary onset preeclampsia, late onset preeclampsia and normal pregnant woman

Objectives: This study aimed to compare the serum levels of soluble Endoglin (s-Eng) between early onset preeclampsia, late onset preeclampsia and normal pregnant women.Materials and Methods: This was an analytic observational study (Cross-Sectional) performed on 39 pregnant women with early-onset preeclampsia (EO-PE), late-onset preeclampsia (LO-PE), and normal pregnancy. The patients were consecutively chosen in Dr. Soetomo Hospital, Airlangga University Hospital and Dr. M. Soewandhi Hospital Surabaya in May-July 2016. The serum concentration of soluble Endoglin were collected by venous puncture taken from maternal circulation and measured by ELISA.Results: From this study, serum concentrations of soluble Endoglin was higher significantly on the early onset preeclampsia compared with late onset preeclampsia and normal pregnancy (47,65 ± 40,17 vs 13,46 ± 9,48 vs 6,11 ± 1,45 ng/mL; p=0.000). Conclusion: This study shows angiogenic imbalance was more prominent compared in early-onset than late-onset preeclampsia. This may be because the placental dysfunction, placental ischemia, which produce excessive anti angiogenic factors, whic later causing endothelial dysfunction was more related to early onset preeclampsia.

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The Difference in Maternal Serum Hypoxia-Inducible Factors-1α Levels between Early Onset and Late-Onset Preeclampsia

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.601 ◽

2019 ◽

Vol 7 (13) ◽

pp. 2133-2137 ◽

Cited By ~ 1

Author(s):

Roza Sriyanti ◽

Johanes C. Mose ◽

Masrul Masrul ◽

Netti Suharti

Keyword(s):

Early Onset ◽

Late Onset ◽

Serum Levels ◽

Normal Pregnancy ◽

Maternal Serum ◽

Hypoxia Inducible Factors ◽

Cross Sectional ◽

Significant Difference ◽

The Difference ◽

Early Onset Preeclampsia

BACKGROUND: Preeclampsia can be divided into early (EOPE) and late (LOPE) onset preeclampsia. Preeclampsia is related to the failure of placentation. Accumulation of hypoxia-inducible factors (HIF)-1α is commonly an acute and beneficial respond to hypoxia, while chronically elevated is associated with preeclampsia. AIM: This study aims to evaluate the serum levels of HIF-1α in preeclampsia and normal pregnancy, and to compare the difference between early-onset and late-onset preeclampsia. METHODS: A cross-sectional comparative study was conducted among a total of 69 pregnant women at ≥ 20 weeks of gestation, were recruited at obstetrics and gynaecology department at Dr M. Djamil Padang Hospital, network hospitals, health centres. They were divided into three groups early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy. Preeclampsia was diagnosed using International Guidelines. Data were analysed by SPSS 24 program; data are presented as median and range or as mean ± standard deviation. One-way ANOVA test was used to determine the relationship between HIF-1α levels with the onset of preeclampsia. RESULTS: The results showed that the mean maternal serum HIF-1α levels in early-onset preeclampsia (EOPE), late-onset preeclampsia (LOPE), and normal pregnancy were 1366.96 ± 733.40 pg/ml, 916.87 ± 466.06 pg/ml, and 716.77 ± 541.08 pg/ml. Serum HIF-1α levels were higher in early-onset preeclampsia (EOPE), and late-onset preeclampsia (LOPE) compared to normal pregnancy. Among preeclampsia patients, serum HIF-1α was higher in EOPE than LOPE women. Statistical analysis revealed a significant difference in mean maternal serum HIF-1α between early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy (p < 0.05). CONCLUSION: This study concluded that there is a significantly different level of HIF-1α between in early-onset preeclampsia, late-onset preeclampsia and normal pregnancy. Early-onset preeclampsia is the highest levels of serum HIF-1α.

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Differences in levelFms-Like Tyrosine Kinase-1 (sFlt-1), soluble Endoglin (s-Eng), and Placental Growth Factor (PIGF) between Early Onset Preeclampsia and Late Onset Preeclampsia

Journal of Midwifery ◽

10.25077/jom.3.2.11-18.2018 ◽

2018 ◽

Vol 3 (2) ◽

pp. 11

Author(s):

Lita Nafratilova ◽

Yusrawati Yusrawati ◽

Irza Wahi

Keyword(s):

Early Onset ◽

Late Onset ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Cross Sectional ◽

Intrinsic Factors ◽

Significant Difference ◽

The Difference ◽

Cross Sectional Design ◽

Early Onset Preeclampsia

Early Onset Preeclampsia (EO-PE) is preeclampsia that develops before 34 weeks 'gestation, caused by intrinsic factors, while Late Onset Preeclampsia (LO-PE) is preeclampsia that develops after 34 weeks' gestation due to extrinsic and maternal factors. There is an increased production of antiangiogenic factors (sFlt-1, s-Eng and PIGF) contribute to pathophysiology of preeclampsia.This study aims to measure the difference of sFlt-1, sEng, PIGF levels between EO-PE and LO-PE. This was an observational study with cross sectional design conducted at Dr. M. Djamil, TK Hospital. III dr. Reksodiwiryo and Biomedical Laboratory FK Unand Padang from August 2017 to August 2018. The sample of this study were 26 severe preeclampsia women : 13 (EO-PE) and 13 (LO-PE), selected using consecutive sampling. Levels of sFlt-1, sEng, PIGF were examined using the enzyme-linked immunosorbent assay (ELISA) method. Statistical analysis was performed using unpaired t test and Mann-Whitney Test. Results shown that serum levels of sFlt-1 and sEng in (EO-PE) were 9.51 ± 0.71 ng / L, 1.44 ± 0.06 ng / mL, 5.79 ± 0.42 ng / mL while in PEAL it was 8, 89 ± 0.78 ng / mL, 1.35 ± 0.14 ng / mL, 6.72 ± 0.76. There were a significant difference with a value of p <0.05. The conclusion of this study is that the levels of sFlt-1 and sEng are higher in (EO-PE) than(LO-PE)and PIGF levels was lower in (EO-PE) compared to (LO-PE)

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Perbedaan Rerata Kadar Soluble Fms-Like Tyrosine Kinase-1 (Sflt-1) Serum pada Penderita Early Onset, Late Onset Preeklampsia Berat / Eklampsia dan Kehamilan Normal

Jurnal Kesehatan Andalas ◽

10.25077/jka.v5i1.440 ◽

2016 ◽

Vol 5 (1) ◽

Author(s):

Laila Rahmi ◽

Rahmatina B. Herman ◽

Yusrawati Yusrawati

Keyword(s):

Growth Factor ◽

Tyrosine Kinase ◽

Early Onset ◽

Late Onset ◽

Serum Levels ◽

Normal Pregnancy ◽

Severe Preeclampsia ◽

Cross Sectional ◽

Post Hoc ◽

Endothelial Growth Factor

AbstrakPreeklampsia merupakan sumber utama morbiditas dan mortalitas ibu di seluruh dunia. Kegagalan pengaturan dan ketidakseimbangan agen vasoaktif proangiogenik dan antiangiogenik plasenta, soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF) dan placental growth factor (PlGF) memainkan peran penting dalam patogenesis preeklampsia. Tujuan penelitian ini adalah menentukan perbedaan rerata kadar sFlt-1 serum pada penderita early onset, late onset preeklampsia berat/ eklampsia dan kehamilan normal. Penelitian dilakukan di RSUP Dr. M. Djamil, RS TK. III dr. Reksodiwiryo dan Laboratorium Biologi Molekuler Fakultas Kedokteran Universitas Andalas Padang dari Februari sampai Desember 2014 dengan desain cross sectional. Subjek berjumlah 84 orang, terdiri dari tiga kelompok, yaitu kelompok early onset preeklampsia berat/ eklampsia, late onset preeklampsia berat/ eklampsia, dan kehamilan normal sebagai kelompok kontrol yang diambil dengan teknik consecutive sampling. Darah dikumpulkan dari subjek penelitian dengan cara intravena kemudian diukur dengan metode ELISA. Rerata kadar sFlt-1 pada kelompok early onset, late onset preeklampsia berat/ eklampsia dan kehamilan normal secara berturut-turut adalah 4,69±0,96 ng/ml, 2,39±0,57 ng/ml, dan 1,23±0,42 ng/ml. Perbedaan ini sangat signifikan dengan uji statistik ANOVA (p<0,05) dan uji Post Hoc Test Multiple Comparisons. Kesimpulan penelitian adalah terdapat perbedaan yang sangat signifikan antara kadar sFlt-1 serum pada kelompok early onset preeklampsia berat/ eklampsia, late onset preeklampsia berat/ eklampsia dan kehamilan normal.Kata kunci: sFlt-1, antiangiogenik, preeklampsia berat/ eklampsia, kehamilan normal AbstractPreeclampsia is a major cause maternal morbidity and mortality in the world. Failure regulation and imbalance of vasoactive agents and antiangiogenic proangiogenik placenta, soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) have an important role in the pathogenesis of preeclampsia. The objective of this study was to determine the differences between the mean serum levels of sFlt-1 in patients with early onset, late onset severe preeclampsia/eclampsia and normal pregnancy. This study was conducted in Dr. M. Djamil hospital, dr. Reksodiwiryo TK. III hospital and Biology Moleculer Laboratory Medicine Faculty of Andalas University Padang from February until December 2014 with a cross sectional design. The total subjects were 84 persons, consist of three groups, there was early onset severe preeclampsia/ eclampsia, late onset severe preeclampsia/ eclampsia and normal pregnancy as control group. The subjects were selected by consecutive sampling technique. The blood was collected by intravenous, then sFlt-1 serum measured by ELISA. The mean levels of sFlt-1 in the early onset group, late onset severe preeclampsia/ eclampsia group and normal pregnancy group were 4.69±0.96 ng/ml, 2.39±0.57 ng/ml and 1.23±0.42 ng/ml. This difference very significant by ANOVA statisctical test (p<0,05) and Multiple Comparisons Post Hoc Test. The conclusion of this study is very significant differences between serum levels of sFlt-1 in early onset severe preeclampsia/ eclampsia group, late onset severe preeclampsia/ eclampsia on normal pregnancy.Keywords: sFlt-1, antiangiogenic, severe preeclampsia/ eclampsia, normal pregnancy

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The correlation between Leptin Levels and Onset of Preeclampsia

JOURNAL OBGIN EMAS ◽

10.25077/aoj.4.2.170-175.2020 ◽

2020 ◽

Vol 4 (2) ◽

pp. 170-175

Author(s):

Roza Sriyanti ◽

Johanes C. Mose ◽

Masrul Masrul ◽

Netti Suharti

Keyword(s):

Early Onset ◽

Sampling Method ◽

Late Onset ◽

Normal Pregnancy ◽

Statistical Test ◽

Elisa Method ◽

Exclusion Criteria ◽

Cross Sectional ◽

Consecutive Sampling ◽

Early Onset Preeclampsia

The purpose of this study is to find the correlation between leptin levels and the onset of preeclampsi. This study used a cross sectional comparative study design that conducted in May 2018 - April 2019 in the SMF / Obstetrics and Gynecology department of RSUP dr. M. Djamil Padang, RSUD Achmad Mochtar, RSUD Solok, RST Reksodiwiryo. We used consecutive sampling method which consists of 69 pregnant women who fulfill the inclusion and exclusion criteria. Leptin level tests were done using ELISA method. The average level of leptin in early-onset preeclampsia is found to be the highest when compared to the late-onset preeclampsia and normal pregnancy, 64.07 ± 78.27 vs. 30.46 ± 31.99 vs. 16.61 ± 24.49. This differentiation is highly significant with the ANOVA statistical test (p <0.05). There is a significant correlation between leptin levels with the onset of preeclampsia.Keywords: preeclampsia early onset, preeclampsia late onset, leptin levels

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The correlation between Leptin Levels and Onset of Preeclampsia

JOURNAL OBGIN EMAS ◽

10.25077/aoj.4.2.139-144.2020 ◽

2020 ◽

Vol 4 (2) ◽

pp. 139-144

Author(s):

Roza Sriyanti ◽

Johanes C. Mose ◽

Masrul Masrul ◽

Netti Suharti

Keyword(s):

Early Onset ◽

Sampling Method ◽

Late Onset ◽

Normal Pregnancy ◽

Statistical Test ◽

Elisa Method ◽

Exclusion Criteria ◽

Cross Sectional ◽

Consecutive Sampling ◽

Early Onset Preeclampsia

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Serum levels of interleukin-8 in normal pregnancy, gestational hypertension, late-onset preeclampsia, early-onset preeclampsia, and HELLP syndrome

Journal of Reproductive Immunology ◽

10.1016/j.jri.2016.10.082 ◽

2016 ◽

Vol 118 ◽

pp. 137

Author(s):

Akihide Ohkuchi ◽

Chikako Hirashima ◽

Shiho Nagayama ◽

Kayo Takahashi ◽

Hirotada Suzuki ◽

...

Keyword(s):

Early Onset ◽

Hellp Syndrome ◽

Late Onset ◽

Gestational Hypertension ◽

Serum Levels ◽

Normal Pregnancy ◽

Interleukin 8 ◽

Early Onset Preeclampsia

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PERBANDINGAN KADAR IFN-γ DAN IL-10 PADA SERUM MATERNAL ANTARA EARLY-ONSET PREECLAMPSIA DENGAN LATE-ONSET PREECLAMPSIA

Jurnal Biosains Pascasarjana ◽

10.20473/jbp.v17i2.2015.98-104 ◽

2015 ◽

Vol 17 (2) ◽

pp. 98

Author(s):

Siti Nur Khalida

Keyword(s):

Early Onset ◽

Late Onset ◽

Cross Sectional ◽

Ifn Γ ◽

Early Onset Preeclampsia

AbstrakPenelitian ini bertujuan untuk membandingkan kadar IFN-γ dan IL-10 pada serum maternal antara early-onset preeclampsia dengan late-onset preeclampsia. Penelitian ini dilakukan di RSUD dr.Mohammad Suwandhie Surabaya sejak Juni – Agustus 2015, menggunakan rancangan cross-sectional. Didapatkan 13 sampel early-onset preeclampsia, 13 sampel late-onset preeclampsia, 13 sampel hamil normal <34 minggu dan 13 sampel hamil normal ≥34 minggu, total 52 sampel serum yang kemudian dilakukan pemeriksaan IFN-γ dan IL-10 serum dengan ELISA. Berdasarkan hasil analisis statistik, didapatkan nilai median kadar IFN-γ pada early-onset preeclampsia 0 pg/ml (0-149,1 pg/ml), pada late-onset preeclampsia 0 pg/ml, pada kelompok kontrol early 0 pg/ml (0-56,6 pg/ml), dan pada kelompok kontrol late 0 pg/ml (0-92,7 pg/ml). Hal ini kemungkinan terjadi karena kadar IFN-γ pada sampel lebih rendah dari ambang batas terendah deteksi kit ELISA IFN-γ human (R&D system inc). Sementara itu, nilai median kadar IL-10 pada early-onset preeclampsia adalah 91 pg/ml (6,2-163,90 pg/ml), pada late-onset preeclampsia 12,9 pg/ml (3,5 – 110,70 pg/ml), pada kontrol early 8,9 pg/ml (0-36,5 pg/ml) dan pada kontrol late 4,8 pg/ml (0-38,8 pg/ml) Secara statistik, tidak terdapat perbedaan bermakna kadar IFN-γ antara early-onset preeclampsia dengan late-onset preeclampsia, begitu pula dengan kelompok kontrol (harga p = 0,073). Sedangkan, menurut statistik didapatkan perbedaan bermakna kadar IL-10 antara early-onset preeclampsia dengan late-onset preeclampsia, begitu pula dengan kelompok kontrol (harga p <0,0001). Kesimpulan, tidak terdapat perbedaan bermakna kadar IFN-γ baik pada kelompok early-onset preeclampsia maupun late-onset preeclampsia, sedangkan kadar IL-10 pada early-onset preeclampsia lebih tinggi dari pada late-onset preeclampsia. Kata kunci: early-onset preeclampsia, late-onset preeclampsia, preeklampsia, IFN-γ, IL-10

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Placental Growth Factor Level is Lower in Early-Onset Preeclampsia, while Tumor Necrosis Factor Alpha Level does not Show any Difference between Early and Late Onset Preeclampsia

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v36i4.322 ◽

2016 ◽

Author(s):

Christofani Ekapatria

Keyword(s):

Serum Level ◽

Early Onset ◽

Late Onset ◽

Necrosis Factor Alpha ◽

Cross Sectional ◽

District Hospitals ◽

Tnf Α ◽

Kolmogorov Smirnov ◽

The Difference ◽

Early Onset Preeclampsia

Objective: To analyze the difference of PlGF and TNF-α serum level between early-onset and late-onset preeclampsia. Method: This is a cross-sectional analytic comparative study comparing serum level of PlGF and TNF-α between groups with earlyand late-onset preeclampsia. Each group consists of 32 subjects who met inclusion criteria and presented to Dr. Hasan Sadikin Hospital or its district hospitals in September - November 2012. Statistical analysis was performed with Kolmogorov Smirnov test, Saphiro-Wilk test, and non-parametric Mann-Whitney test. Result: Mean of PlGF serum level in the group with early-onset preeclampsia is 53.0344±38.07140 pg/ml, while mean of which in the group with late-onset preeclampsia is 241.8063±192.8373 pg/ml (p

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Differences Ratio Level Soluble FMS-Like Tyrosine Kinase-1 and Placental Growth Factor Early And Late Onset on Preeclampsia and Normal Pregnancy

Journal of Midwifery ◽

10.25077/jom.1.1.83-92.2018 ◽

2018 ◽

Vol 3 (1) ◽

pp. 83

Author(s):

Ria Andina ◽

Yanwirasti Yanwirasti ◽

Defrin Defrin

Keyword(s):

Growth Factor ◽

Tyrosine Kinase ◽

Early Onset ◽

Late Onset ◽

Normal Pregnancy ◽

Elisa Test ◽

Placental Growth Factor ◽

Cross Sectional ◽

Onset Group ◽

Early Onset Group

Preeclampsia is a major maternal morbidity and mortality worldwide including in Indonesia. PIGF concentrations were found to be lower and sFlt-1 to be higher in patients with preeclampsia than normal pregnancy. Futher, this factor has been proposed as a parameter that can help identify women with potentially preeclampsia.This study aims todeterminethe differences ratio level soluble rate fms-like tyrosine kinase-1 and placental growth factor early and late onset on preeclampsia and normal pregnancy. The cross sectional study design was conducted in RSUP M.Djamil, Rasidin Hospital, Reksodiwiryo Hospital and Biomedical Laboratory of Andalas University from July 2017 until January 2018. The sample of this study was pregnant women>20-42 weeks pregnancy totalling 80 people by consecutive sampling.Sample was divided into 3 groups. SFlt-1 and PlGF levels tested using ELISA test. Test the normality of data by Kolmogrov-Smirnov test by using unpaired T test.The results showed median sFLT-1 levels in the early onset group with normal pregnancy with p= 0,88, median sFLT-1 levels in the late onset group with normal pregnancy with p= 0,01 and median levels of sFLT-1 in the early onset group with late onset with p= 0.34. Mean of PlGF in the early onset group with normal pregnancy with p=0,30, mean of PlGF in the late onset group with normal pregnancy with p= 0.63, and mean of PlGF in the early onset group with late onset with p = 0.27. The conclusion of this study was that there was a difference ratio level Soluble Fms-Like Tyrosine Kinase-1 late onset in preeclampsia and normal pregnancy.

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