scholarly journals The effect of sambiloto tablet (AS201-01) on placental Chondroitin Sulfate A (CSA) expression of pregnant mice infected by Plasmodium berghei

2018 ◽  
Vol 26 (2) ◽  
pp. 83
Author(s):  
Nasrul Wahdi ◽  
Widjiati Widjiati ◽  
Aty Widyawaruyanti ◽  
Budi Prasetyo
Keyword(s):  
Experimental Study ◽  
Placebo Group ◽  
Chondroitin Sulfate ◽  
Plasmodium Berghei ◽  
Drug Group ◽  
The Other ◽  
Treatment Groups ◽  
Pregnant Mice ◽  
Chondroitin Sulfate A

Objectives: To determine the effect of Sambiloto tablet (AS201-01) in reducing the placental Chondroitin Sulfate A (CSA) Expression of pregnant mice infected Plasmodiumberghei.Materials and Methods: Experimental study using 24 pregnant mice were divided into 4 groups with randomization. Uninfected group, the placebo group, the Sambiloto tablet (AS201-01) group and the DHP tablet (as a standart drug) group. The last three groups, were infected with P. bergheion day 9th of pregnancy, and the treatment was started at day 11th of pregnancy, and samples were terminated at day 15th of pregnancy by surgery. Placental sampling were stained with Tunnel assay to measure placental CSA antibodies.Results: The placental Chondroitin Sulfate A (CSA) expression. Uninfected group compared to Sambiloto tablet (AS201-01) groups was not significantly different (p>0.05), uninfected group compared with the other treatment groups differ meaningfully (p<0.05). Placebo group compared with all groups significantly different (p<0.05). Sambiloto tablet (AS201-01) group compared to uninfected group (p>0.05) was not significantly different, with another group was significantly different (p<0.05). DHP tablet group compared to all the groups was significantly different (p<0.05).Conclusion: Placental Chondroitin Sulfate A (CSA)expression of mice infected by Plasmodium berghei treated with Sambiloto tablet (AS201-01) lower than DHP tablet. 

Threat as a Determinant of Alcohol-Related Aggression

1986 ◽  
Vol 58 (1) ◽  
pp. 287-297 ◽  
Author(s):  
Roland Gustafson
Keyword(s):  
Experimental Study ◽  
Body Weight ◽  
Placebo Group ◽  
Psychological Factors ◽  
Aggressive Response ◽  
The Other ◽  
Partner Aggression ◽  
Alcohol Group ◽  
Model Combining ◽  
Pure Alcohol

An experimental study was performed within a modified version of the Buss “aggression-machine” paradigm testing the hypothesis that threat will inhibit aggression in intoxicated subjects given that threat is not unavoidable. 40 males were randomly assigned to either an alcohol group, drinking .08 ml of pure alcohol per kg body weight, or a placebo group, drinking only tonic. Half of the subjects in each group were exposed to a threat and the other half to no threat. At 10 opportunities subjects could choose between a nonaggressive response or an aggressive response in the form of an electric shock given to a bogus partner. Aggression was estimated in terms of number of aggressive responses, the intensity and duration of these responses, and response latency. Results clearly confirmed the hypothesis and were discussed theoretically in a model combining pharmacological and psychological factors.

scholarly journals The Effects of Borax (NA2B4O710H2O) on Histopathology of Wistar Rats’ Cerebrum (Rattus norvegicus)

2021 ◽  
Vol 11 (3) ◽  
pp. 484-488
Author(s):  
Fernanda Elfan ◽  
Suryo Kuncorojakti ◽  
Nusdianto Triakoso
Keyword(s):  
Experimental Study ◽  
Statistical Analysis ◽  
Pyramidal Cell ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
The Other ◽  
Control Treatment ◽  
Cell Necrosis ◽  
Treatment Groups ◽  
Significant Difference

The present study aimed to determine the effects of borax (Na2B4O7.10H2O) addition on the changes of histological cerebrum imaging in the brains of white mice (Rattus norvegicus). The current research was an experimental study with randomization of 24 white mice that were divided into four treatment groups with five replications. Borax was dissolved for each treatment with a dose of 19 mg/mouse/day, 26 mg/mouse/day, and 37 mg/mouse/day, and it was administered orally for 14 days. Then, it was analyzed statistically using the Kruskal-Wallis test. The statistical analysis results suggested that there were significantly different results in each treatment group. The control treatment with an administration dose of 26 mg/rat/day had a significantly different result in the worst cloudy swelling degeneration of cerebrum in histopathology imaging on Wistar rats (Rattus norvegicus). Using the Mann-Whitney test, it was found that the dose of borax at 37 mg/rat/day led to significant difference, compared to the other treatment groups, which means that 37 mg/rat/day of borax caused the worst pyramidal cell necrosis in histopathology imaging of the cerebrum on white mice. Borax exposure on Wistar rats (Rattus norvegicus) can cause cloudy swelling at a dose of 26mg/head/day, and pyramidal cell necrosis at a dose of 37 mg/head/day.

scholarly journals Comparison of the effect of sambiloto (AS 201-01) tablet and dihydroartemisinin-piperaquine on macrophage MIF expression in mice placenta infected with Plasmodium berghei

2019 ◽  
Vol 26 (3) ◽  
pp. 100
Author(s):  
Desak Ketut Ayu Aryani ◽  
Budi Prasetyo ◽  
Aty Widyawaruyanti ◽  
Widjiati Widjiati
Keyword(s):  
Placebo Group ◽  
Plasmodium Berghei ◽  
Immunohistochemical Staining ◽  
Laboratory Research ◽  
Experimental Laboratory ◽  
Pregnant Mice

Objective: To compare the administration of sambiloto tablets (AS201-01) and dihydroartemisinin-piperaquine phosphate tablets in pregnant mice infected by P. berghei on the expression of MIF in the placenta.Materials and Methods: Experimental laboratory research, 24 pregnant mice were divided into 4 groups with randomization, ie. non-infected, placebo, sambiloto (AS201-01) and DHP groups. On day 9 P. berghei was infected, on day 11 the treatment was given, day 15 the surgery was performed, placental samples were taken, immunohistochemical staining was given, and MIF expression was assessed.Results: The expression of MIF in the group not infected with P. berghei had the lowest mean, while the highest mean was found in the placebo group. Uninfected groups were not significantly different from sambiloto (AS201-01) tablet group. Sambiloto tablet group (AS201-01) had lower MIF expression than DHP group, but it was not significantly different. Sambiloto tablet group (AS201-01) showed lower MIF expression than placebo. MIF expression in DHP group was lower than that in placebo group. From the lowest, the MIF expressions were as follows: group not infected with P. berghei, group receiving sambiloto (AS201-01) tablet, DHP group and placebo group.Conclusion: MIF expression in the placenta of pregnant mice infected with P. berghei and receiving sambiloto (AS20-01) tablets was not different from those receiving DHP tablets.

TERATOGENIK EKSTRAK ETANOL UWI BANGGAI UNGU (Dioscorea alata L.) PADA MENCIT BETINA (Mus musculus)

2020 ◽  
Vol 5 (2) ◽  
pp. 309-318
Author(s):  
Ihwan Ihwan ◽  
◽  
Rahmatia Rahmatia ◽  
Khildah Khaerati ◽  
Keyword(s):  
Treatment Group ◽  
Embryo Development ◽  
Mus Musculus ◽  
Birth Defect ◽  
Abnormal Development ◽  
Dioscorea Alata ◽  
Treatment Groups ◽  
Study Results ◽  
Pregnant Mice ◽  
Ethanol Extracts

Teratogenic is an abnormal development on embryo and is the cause of congenital defect or birth defect. This study aims to determine the effect of the addition of Dioscorea alata L. ethanol extracts to the embryo development on pregnant mice whose given orally to 24 mice which divided to 4 treatment groups, they are the normal group (NG) with NaCMC 0.5%; 28 mg/KgBB treatment group; 35 mg/KgBB; 42 mg/Kg BB. The addition of Dioscorea alata L ethanol extracts was done on the sixth day until the 15th day of pregnancy. On the 18th day of pregnancy, Laparaktomi was done to the pregnant mice and the embryo was taken out of the uterus. The observation was done to the fetus numbers, weight weighing of the fetus's body, dan length measurement of the fetus's body. Another observation is the observation of the external organ defect of the embryo. The study results that the addition of Dioscorea alata L ethanol extracts with various doses have no significant effect (P>0.5) to the mice external fetus development. On the examination of the fetus, we can conclude that Dioscorea alata L ethanol extracts don’t give any effect that may cause the defect of the fetus’ external organ.

scholarly journals Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ning Li ◽  
Yichi Zhang ◽  
Narayan Nepal ◽  
Guoqing Li ◽  
Ningning Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Body Weight ◽  
Stem Cell ◽  
Dental Pulp ◽  
The Other ◽  
Therapeutic Effects ◽  
Colon Injury ◽  
Treatment Groups ◽  
Hepatocyte Growth

Abstract Background Ulcerative colitis (UC) is a chronic and recurrent disease without satisfactory treatment strategies. Dental pulp stem cell (DPSC) transplantation has been proposed as a potential therapy for UC. This study aimed to investigate the therapeutic effects of the rat hepatocyte growth factor (HGF) gene transduced into DPSCs for UC. Methods The therapeutic effects of HGF-DPSCs transplanted intravenously into a rat model of UC induced by 5% dextran sulphate sodium (DSS) were compared with the other treatment groups (LV-HGF group, DPSCs group and GFP-DPSCs group). Immunofluorescence and immunohistochemistry were used to observe the localization and proliferation of HGF-DPSCs at the site of colon injury. The expression levels of inflammatory factors were detected by real-time quantitative PCR (RT-PCR) and western blotting. The oxidative stress markers were detected by ELISA. DAI scores and body weight changes were used to macroscopically evaluate the treatment of rats in each group. Results Immunofluorescence and immunohistochemistry assays showed that HGF-DPSCs homed to colon injury sites and colocalized with intestinal stem cell (ISC) markers (Bmi1, Musashi1 and Sox9) and significantly promoted protein expression (Bmi1, Musashi1, Sox9 and PCNA). Anti-inflammatory cytokine (TGF-β and IL-10) expression was the highest in the HGF-DPSCs group compared with the other treatment groups, while the expression of pro-inflammatory cytokines (TNF-α and INF-γ) was the lowest. Additionally, the oxidative stress response results showed that malondialdehyde (MDA) and myeloperoxidase (MPO) expression decreased while superoxide dismutase (SOD) expression increased, especially in the HGF-DPSCs group. The DAI scores showed a downward trend with time in the five treatment groups, whereas body weight increased, and the changes were most prominent in the HGF-DPSCs group. Conclusions The study indicated that HGF-DPSCs can alleviate injuries to the intestinal mucosa by transdifferentiating into ISC-like cells, promoting ISC-like cell proliferation, suppressing inflammatory responses and reducing oxidative stress damage, which provides new ideas for the clinical treatment of UC.

Effectiveness of Virtual Resilience Training on Assertiveness in Student Girls Aged 9–10 Years: A 1-Month Follow-Up

2021 ◽  
pp. 105984052110135
Author(s):  
Shima Gadari ◽  
Jamile Farokhzadian ◽  
Parvin Mangolian Shahrbabaki
Keyword(s):  
Experimental Study ◽  
Internal Stress ◽  
Intervention Group ◽  
The Other ◽  
Hormonal Changes ◽  
Emotional Instability ◽  
Other Hand ◽  
Significant Difference ◽  
Resilience Training

Girls between the ages of 9 and 10 begin to experience physical, physiological, and hormonal changes that may lead to internal stress. At this age, children are struggling for autonomy; on the other hand, they may experience emotional instability, and for these reasons, they may be vulnerable in many ways. This experimental study aimed to investigate the effect of resilience training on assertiveness in student girls aged 9–10. Data were collected before, immediately after, and 1 month after the intervention in the control ( n = 40) and intervention ( n = 37) groups. There was a significant difference between the assertiveness of the intervention immediately (26.80 ± 3.73) and 1 month after the intervention (27.05 ± 3.73), and assertiveness significantly increased in the intervention group ( p = .0001). Resilience training leads to improvements in assertiveness in student girls aged 9–10.

Study of wave attenuation in concrete

1993 ◽  
Vol 8 (9) ◽  
pp. 2344-2353 ◽  
Author(s):  
J-M. Berthelot ◽  
Souda M. Ben ◽  
J.L. Robert
Keyword(s):  
Experimental Study ◽  
Plane Wave ◽  
Wave Attenuation ◽  
Plane Waves ◽  
Propagation Distance ◽  
Experimental Results ◽  
The Other ◽  
Wave Frequency ◽  
Concrete Composition ◽  
Analytical Expression

The experimental study of wave attenuation in concrete has been achieved in the case of the propagation of plane waves in concrete rods. Different mortars and concretes have been investigated. A transmitter transducer coupled to one of the ends of the concrete rod generates the propagation of a plane wave in the rod. The receiver transducer, similar to the previous one, is coupled to the other end of the rod. The experimental results lead to an analytical expression for wave attenuation as function of the concrete composition, the propagation distance, and the wave frequency.

Reproductive performance of group-synchronized Merino d'Arles and Romanov crossbred ewes. 1. A note on the effects of lactation on fecundity

1977 ◽  
Vol 24 (1) ◽  
pp. 113-116 ◽  
Author(s):  
F. J. Galindez ◽  
M. Prud'hon ◽  
G. Reboul
Keyword(s):  
Treatment Group ◽  
Reproductive Performance ◽  
The Other ◽  
Fertility Level ◽  
Homogeneous Groups ◽  
Treatment Groups ◽  
Significant Superiority

SUMMARYFifty-six Merino d'Aries and 56 Romanov × Merino crossbred lactating ewes were divided into two treatment groups, one (T1) with a ewe-to-ram ratio of 48: 1 (24 Merino and 24 crossbred) and the other (T2) of 64: 1 (32 Merino and 32 crossbred).Intravaginal sponges with 40 mg FGA (Searle) were inserted in two subgroups at 8-day intervals and withdrawn after 12 and 16 days in situ, in four equal groups at 4-day intervals. A dose of 400 IU pregnant mare's serum gonadotrophin (PMSG) was applied at sponge withdrawal.Fertility after two oestrous cycles was equally satisfactory in both treatments, though it was lower in treatment group T2. This could be due to the significantly low (P<0·01) number of Merino ewes raddled by the T2 ram (0·33 Merino against 0·92 crossbred) suggesting preferential behaviour by the ram in this treatment.Comparisons between breeds show a slight superiority in the fertility of crossbred ewes and a highly significant superiority in prolificacy These results suggest that using the described method, 2% of rams seems more than adequate to mate with progestagen-synchronized ewes with a satisfactory fertility level. It is, however, recommended to use homogeneous groups of ewes.

Experimental Study on Racetrack-Shaped Holes Impingement Cooling With Bump Type Roughening Element

2012 ◽  
Author(s):  
Chiyuki Nakamata ◽  
Yoji Okita ◽  
Takashi Yamane ◽  
Yoshitaka Fukuyama ◽  
Toyoaki Yoshida
Keyword(s):  
Experimental Study ◽  
Flow Condition ◽  
Reynolds Numbers ◽  
Main Flow ◽  
The Other ◽  
Relative Location ◽  
Target Plate ◽  
Cooling Effectiveness ◽  
Impingement Cooling ◽  
Cooling Air

Cooling effectiveness of an impingement cooling with array of racetrack-shaped impingement holes is investigated. Two types of specimens are investigated. One is a plain target plate and the other is a plate roughened with bump type elements. Sensitivity of relative location of bump to impingement hole on the cooling effectiveness is also investigated. Experiments are conducted under three different mainflow Reynolds numbers ranging from 2.6×105 to 4.7×105, with four different cooling air Reynolds numbers for each main flow condition. The cooling air Reynolds numbers are in the range from 1.2×103 to 1.3×104.

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