scholarly journals Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ning Li ◽  
Yichi Zhang ◽  
Narayan Nepal ◽  
Guoqing Li ◽  
Ningning Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Body Weight ◽  
Stem Cell ◽  
Dental Pulp ◽  
The Other ◽  
Therapeutic Effects ◽  
Colon Injury ◽  
Treatment Groups ◽  
Hepatocyte Growth

Abstract Background Ulcerative colitis (UC) is a chronic and recurrent disease without satisfactory treatment strategies. Dental pulp stem cell (DPSC) transplantation has been proposed as a potential therapy for UC. This study aimed to investigate the therapeutic effects of the rat hepatocyte growth factor (HGF) gene transduced into DPSCs for UC. Methods The therapeutic effects of HGF-DPSCs transplanted intravenously into a rat model of UC induced by 5% dextran sulphate sodium (DSS) were compared with the other treatment groups (LV-HGF group, DPSCs group and GFP-DPSCs group). Immunofluorescence and immunohistochemistry were used to observe the localization and proliferation of HGF-DPSCs at the site of colon injury. The expression levels of inflammatory factors were detected by real-time quantitative PCR (RT-PCR) and western blotting. The oxidative stress markers were detected by ELISA. DAI scores and body weight changes were used to macroscopically evaluate the treatment of rats in each group. Results Immunofluorescence and immunohistochemistry assays showed that HGF-DPSCs homed to colon injury sites and colocalized with intestinal stem cell (ISC) markers (Bmi1, Musashi1 and Sox9) and significantly promoted protein expression (Bmi1, Musashi1, Sox9 and PCNA). Anti-inflammatory cytokine (TGF-β and IL-10) expression was the highest in the HGF-DPSCs group compared with the other treatment groups, while the expression of pro-inflammatory cytokines (TNF-α and INF-γ) was the lowest. Additionally, the oxidative stress response results showed that malondialdehyde (MDA) and myeloperoxidase (MPO) expression decreased while superoxide dismutase (SOD) expression increased, especially in the HGF-DPSCs group. The DAI scores showed a downward trend with time in the five treatment groups, whereas body weight increased, and the changes were most prominent in the HGF-DPSCs group. Conclusions The study indicated that HGF-DPSCs can alleviate injuries to the intestinal mucosa by transdifferentiating into ISC-like cells, promoting ISC-like cell proliferation, suppressing inflammatory responses and reducing oxidative stress damage, which provides new ideas for the clinical treatment of UC.

scholarly journals Alleviation of Oxidative Stress in Dental Pulp Cells Following 4-Hexylresorcinol Administration in a Rat Model

2021 ◽  
Vol 11 (8) ◽  
pp. 3637
Author(s):  
Jun-Ho Chang ◽  
Dae-Won Kim ◽  
Seong-Gon Kim ◽  
Tae-Woo Kim
Keyword(s):  
Oxidative Stress ◽  
Dental Pulp ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Mandibular Incisor ◽  
Tnf Α ◽  
Total Antioxidant ◽  
Pulp Cells ◽  
Pre Treatment

Damaged dental pulp undergoes oxidative stress and 4-hexylresorcinol (4HR) is a well-known antioxidant. In this study, we aimed to evaluate the therapeutic effects of a 4HR ointment on damaged dental pulp. Pulp cells from rat mandibular incisor were cultured and treated with 4HR or resveratrol (1–100 μM). These treatments (10–100 μM) exerted a protective effect during subsequent hydrogen peroxide treatments. The total antioxidant capacity and glutathione peroxidase activity were significantly increased following 4HR or resveratrol treatment (p < 0.05), while the expression levels of TNF-α and IL1β were decreased following the exposure to 4HR pre-treatment in an in vitro model. Additionally, the application of 4HR ointment in an exposed dental pulp model significantly reduced the expression of TNF-α and IL1β (p < 0.05). Conclusively, 4HR exerted protective effects against oxidative stress in dental pulp tissues through downregulating TNF-α and IL1β.

The Multifaceted Role of Natural Agents in Colitis-Associated Cancer Prevention and Therapy

2021 ◽  
pp. 220-239
Author(s):  
Ashok Kumar Kumar Pandurangan ◽  
Suresh Kumar Anandasadagopan ◽  
Neesar Ahmed
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Colon Cancer ◽  
The Other ◽  
Preclinical Model ◽  
Natural Agents ◽  
Inflammatory Bowel ◽  
Prevention And Therapy

Inflammatory bowel disease (IBD) is comprised of ulcerative colitis (UC) and Crohn's disease (CD) that was recognized by the inflammation in the colon. There are no proper medications are available to control the IBD in patients. NASIDs such as Aspirin, diclofenac, and ibuprofen are widely used to control the inflammation. On the other hand, the untreated prolonged inflammation leads to the development of cancer in the colon termed as colitis-associated cancer or inflammation-driven colon cancer. Oxidative stress and inflammation play key roles in the pathogenesis of colitis-associated cancer. Single dose of azozymethane (AOM) and three cycles of 2% dextran sodium sulfate (DSS) induces colitis-associated cancer (CAC) in mouse. Hence, many natural products were tested in the preclinical model of colitis-associated cancer. Each of these natural agents modulate important signaling pathway to control the colitis-associated cancer (CAC). In this review, the authors tabulated all the natural agents that culminate the colitis-associated cancer in the preclinical models.

Effect of prolonged undernutrition on the recovery and reproductive performance of maiden Merino ewes

1967 ◽  
Vol 7 (24) ◽  
pp. 7 ◽  
Author(s):  
P McInnes ◽  
TJ Grainger ◽  
MD Smith
Keyword(s):  
Body Weight ◽  
Reproductive Performance ◽  
New South ◽  
New South Wales ◽  
The Other ◽  
First Year ◽  
Treatment Groups ◽  
South Wales ◽  
Feeding Trials ◽  
The Mean

Data are presented on the recovery and reproductive performance of 2 1/2-year-old maiden Merino ewes after a prolonged period of undernutrition. The 217 sheep had been hand-fed on a submaintenance ration in pen feeding trials at Glenfield, New South Wales. During the seven months of the trials they had lost 6 kg (28 to 22 kg) body weight. They were transported to Condobolin in south-western New South Wales, divided into two treatment groups and run on good quality pastures. One group was joined immediately (May 1959) and again ten months later, and the other group was mated after six months at Condobolin (in October 1959) and again 12 months later. The ewes recovered rapidly. The mean weight of both groups had reached 30 kg within six weeks and 40 kg within six months. In the first year 73 of the 100 May-mated ewes bore lambs, but only 38 of these lambs were weaned. Ewes bearing lambs had a higher body weight at the start of joining and gained more during joining than the barren ewes. At the other three joinings (October 1959, May 1960, October 1960) lambing percentage was from 86-89 and weaning percentage from 62-69-both normal for the district. The proportion of twin lambs (3-6 per cent) was low. Wool weight in 1959 was not affected by time of mating or by pregnancy.

scholarly journals Administration of garlic and neem in broiler diet for safe meat production

2020 ◽  
Vol 48 (2) ◽  
pp. 116-126
Author(s):  
R Islam ◽  
MM Hossain ◽  
F Nargis ◽  
ME Hossain
Keyword(s):  
Body Weight ◽  
Basal Diet ◽  
Feed Additives ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Meat Production ◽  
Broiler Chicks ◽  
Treatment Groups ◽  
Broiler Production

This study was conducted to observe the effect of different administration method of garlic and neem on growth performances, feed efficiency and serum biochemical parameters for safe broiler production. The experiment was done for a period of 32 days with 384 one-day-old straight run broiler chicks. The broiler chicks were divided into six groups each of 64 birds and replicated to four subgroups each of 16 birds. The dietary groups were; control (basal diet; no additives), antibiotic (basal diet + antibiotic), garlic in feed (basal diet + 0.25% garlic powder), garlic in water (basal diet + garlic extracts), neem in feed (basal diet + 0.25% neem powder) and neem in water (basal diet + neem extracts). Results showed that the body weight and body weight gain increased significantly (P<0.05) in both the garlic and neem groups compared to the control group. Feed intake was not different (P>0.05) among the treatment groups. Garlic in feed and water and neem in feed groups showed better FCR (P<0.05) compared to the other treatment groups. There were no significant (P>0.05) difference in meat yield, bone development and dressing parameters except head and gizzard in different dietary groups. Supplementation of garlic with feed and water significantly (P<0.05) decrease cholesterol, triglyceride and LDL compared to the other groups. However, both the garlic and neem groups showed numerically higher HDL compare to the control group. There were no 0significant (P>0.05) differences in serum glucose and GPT among different treatment groups. GOT was significantly (P<0.05) lower in neem groups than that of garlic and antibiotic groups. Additive groups showed higher profitability than control group. Based on the results of the study, it may be suggested that the garlic and neem could be used both in feed and water as potential feed additives for safe broiler production. Bang. J. Anim. Sci. 2019. 48 (2): 116-126

scholarly journals Effects of Mesenchymal Stem Cell-derived Exosomes on Oxidative Stress Responses in Skin Cells

2021 ◽  
Author(s):  
Takaaki Matsuoka ◽  
Keita Takanashi ◽  
Katsuaki Dan ◽  
Kenichi Yamamoto ◽  
Koji Tomobe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cellular Senescence ◽  
Stress Responses ◽  
Dermal Fibroblasts ◽  
Skin Fibroblasts ◽  
Therapeutic Effects ◽  
P53 Expression ◽  
Before And After

Abstract Background: The mechanism by which reactive oxygen species (ROS) produced by oxidative stress promote cellular senescence has been studied in detail. This study aimed to verify the preventive or therapeutic effects of mesenchymal stem cell-derived exosomes (MSC-Ex) on the production of ROS induced by oxidative stress in human skin fibroblasts and clarify the mechanisms that promote cellular senescence.Methods: In a system where H2O2 was applied to skin fibroblasts, we assessed the effects of the application of MSC-Ex before and after oxidative stress and measured the fluctuations in several signaling molecules involved in subsequent intracellular stress responses. Exosomes were isolated from MSCs (MSC-Ex) and normal human dermal fibroblasts (NHDFs, NHDF-Ex) before and after exposure to H2O2. NHDFs were treated with exosomes before and after exposure to H2O2.Results: mRNA expression (aquaporin-1 and aquaporin-3) and hyaluronan secretion associated with skin moisturization were reduced by H2O2 treatment, whereas MSC-Ex reversed these effects. The cellular senescence induced by H2O2 was also reproduced in fibroblasts. Specifically, the downregulation of SIRT1 led to increased acetylated p53 expression over time, which induced the expression of p21, a downstream molecule of p53, and arrested the cell cycle, leading to cell senescence. MSC-Ex enhanced these signal transduction systems. MSC-Ex was also effective at blocking the increase of β-galactosidase activity and accumulation of ROS in cells. This effect was stronger than that of NHDF-Ex.Conclusion: MSC-Ex were found to act defensively against epidermal and cellular senescence induced by oxidative stress.

scholarly journals CHARD (BETA VULGARIS VAR. CICLA) EXTRACT MODULATES ZINC STATUS, GLUCOSE LEVEL AND ANTIOXYDANT VALUES IN DIABETIC RATS FED ZINC DEFICIENCY DIET

2017 ◽  
Vol 9 (10) ◽  
pp. 297 ◽  
Author(s):  
Malika Hamdiken ◽  
Zine Kechrid
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Weight Gain ◽  
Beta Vulgaris ◽  
Zinc Deficiency ◽  
Body Weight Gain ◽  
Diabetic Rats ◽  
The Other ◽  
Zinc Status ◽  
Deficient Diet

Objective: Oxidative stress which comes from hyperglycemia, it accelerates the development of cellular and vascular damage complications in diabetes, but the antioxidants may play a beneficial role in its prevention. Several plants extracts have an antioxidant activity and the ability to reduce oxidative stress in diabetes. Thus this study was conducted to investigate the effect of Beta vulgaris var cicla extract on zinc status, glucose concentration and antioxidant parameters in streptozotocin-diabetic rats fed zinc deficiency diet.Methods: Twenty-eight male albino (Wistar) rats were divided into four groups: two groups fed a zinc-sufficient diet one non-diabetic and the other diabetic, while the others two diabetic groups were fed a zinc-deficient diet, one non-treated group and the other treated with the extract of Beta vulgaris var cicla. After 21 d of dietary manipulation, fasting animals were scarified. Blood glucose, tissues zinc (femur, liver, kidney), malondialdehyde (MDA), reduced glutathione (GSH), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) were evaluated.Results: Body weight gain of zinc-deficient diabetic animals was lower than that of zinc-adequate diabetic animals. It was noticed also that inadequate dietary zinc intake increased glucose and MDA levels. In addition, zinc deficiency diet led to a decrease in zinc tissues, GSH concentration both GST and GSH-Px activities. However, Oral administration of Beta vulgaris extract significantly decreased both serum glucose and MDA (p<0.001) levels, with a significant increase in body weight gain (p<0.001), GSH concentration (p<0.05, P<0.001), GST (p<0.05, p<0.001) and GSH-Px (p<0.001) activities.Conclusion: The present study showed that Beta vulgaris var cicla supplementation presumably acting as an antioxidant, and it can be a natural source for the reduction of diabetes development caused by zinc deficiency.

Preventive Effects of an UPLC-DAD-MS/MS Fingerprinted Hydroalcoholic Extract of Citrus aurantium in a Mouse Model of Ulcerative Colitis

Planta Medica ◽  
2018 ◽  
Vol 84 (15) ◽  
pp. 1101-1109 ◽  
Author(s):  
Youn-Hwan Hwang ◽  
Jin Ma
Keyword(s):  
Ulcerative Colitis ◽  
Body Weight ◽  
Intestinal Inflammation ◽  
Clinical Signs ◽  
Pathological Examination ◽  
Array Detector ◽  
Therapeutic Effects ◽  
Citrus Aurantium ◽  
Hydroalcoholic Extract ◽  
Expression Levels

AbstractAlthough traditionally used to improve indigestion, diarrhea, dysentery, and constipation, the therapeutic effects of Citrus aurantium on intestinal inflammation remain unclear. The aim of this study was to evaluate the beneficial effects and to identify the active components of a hydroalcoholic extract of C. aurantium (HECA) on ulcerative colitis. HECA was prepared with 70% ethanol solution in water and extracted at 37 °C for 12 h in triplicate, filtered through a sieve, and lyophilized. Phytochemical identification of HECA was performed by ultra-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS/MS). Animals were randomly assigned to one of four groups based on the treatment conditions. Ulcerative colitis was induced by administration of 2.5% dextran sodium sulfate (DSS) in drinking water for 5 d. Body weight, clinical signs, colon length, pro-inflammatory cytokine expression levels, and histopathological findings were evaluated. In UPLC-DAD-MS/MS analysis, the identified phytochemical components of HECA included four alkaloids, seven coumarins, 18 flavonoids, two lignans, two phenolics, and 10 terpenoids. HECA markedly protected against body weight loss and colon shortening. In pathological examination, HECA alleviated DSS-related mucosal inflammatory lesions in the colon. Moreover, HECA markedly reduced the expression levels of interleukin-6, interferon-γ, tumor necrosis factor-α, and monocyte chemotactic protein-1 in colonic inflammation. Taken together, HECA has potential to relieve mucosal inflammation in the colon, suggesting that the putative active ingredients are responsible for the anti-ulcerative effects.

scholarly journals Does curcumin or metformin attenuate oxidative stress and diabetic nephropathy in rats?

2018 ◽  
Vol 8 (1) ◽  
pp. 8-8 ◽  
Author(s):  
Soheila Asadi ◽  
Mohammad Taghi Goodarzi ◽  
Jamshid Karimi ◽  
Mohammad Hashemnia ◽  
Iraj Khodadadi
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Diabetic Nephropathy ◽  
Oxidative Status ◽  
P Value ◽  
Chow Diet ◽  
Therapeutic Effects ◽  
Urinary Creatinine ◽  
Antioxidant Agents

Background: Since the importance of oxidative stress in the development of diabetic nephropathy (DN) has previously been established, the therapeutic effects of various natural antioxidant agents or synthetic drugs have so far been investigated. Objectives: The aim of this study was to investigate the beneficial effects of curcumin (a natural polyphenol) and metformin (a common therapeutic medicine for type 2 diabetes) on oxidative status in kidney of type 1 diabetic rats. Materials and Methods: In this experimental study 60 male Wistar rats were divided into 10 groups. Type 1 diabetes was induced by streptozotocin. Rats received chow diet and treated with either normal saline in control (N) and diabetic control (D) groups or different doses of metformin (Met) (300 or 500 mg/kg body weight) or curcumin (Cur) (50 or 150 mg/kg body weight) in N+Met300, N+Met500, N+Cur50, N+Cur150, D+Met300, D+Met500, D+Cur50, and D+Cur150 groups. Urinary creatinine, urea, and protein were measured. Total antioxidant capacity (TAC), total oxidant status (TOS), malondialdehyde (MDA), and the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase were assessed in kidney tissues. Results: Both metformin and curcumin showed significant effects on urinary creatinine, urea, and protein levels (P value for all was <0.001). Unlike metformin, curcumin completely restored TAC and TOS (P<0.001), and MDA (P=0.012) in kidney tissues and significantly recovered the activities of SOD (P= 0.003), GPx (P< 0.001), and catalase (P=0.011). Conclusions: Curcumin was found more effective than metformin in attenuating oxidative status in DN.

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