scholarly journals ANTIBACTERIAL EFFECTIVITY TEST OF ULIN BARK EXTRACT (Eusideroxylon zwageri) ON THE GROWTH OF Porphyromonas gingivalis

2021 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Della Isnadya Noor ◽  
I Wayan Arya Krishnawan Firdaus ◽  
Beta Widya Oktiani

ABSTRACTBackground: Chronic periodontitis is an infectious disease caused by bacterial colonization of dental plaque. Bacteria that play a role in chronic periodontitis is Porphyromonas gingivalis. One of mouthwash that reduce the number of P.gingivalis colonies is chlorhexidine 0.2%. Long term use of chlorhexidine 0.2% can cause some side effects to the oral cavity, so we need an alternative mouthwash from natural ingredients that can reduce the side effects of chlorhexidine 0.2%. Ulin bark extract contains phenolic, flavonoid, tannin, alkaloid, terpenoid, and saponin which can be an alternative base for mouthwash besides chlorhexidine 0.2%. Purpose: It is to analyze antibacterial effectivity of ulin bark extract on the growth of Porphyromonas gingivalis. Methods: This study was using true experimental research and post-test only with control group design, that used 9 treatment groups with 4 replications, that were 5%, 10%, 20%, 40%, 60%, 80%, 100% concentrations, chlorhexidine 0.2%, and sterile aquadest against P. gingivalis. The total samples were as many as 36. Result: One Way ANOVA and Post-Hoc Games Howell tests showed that the average absorbance values has a significant difference, then Kruskall Wallis and Post Hoc Mann Whitney tests showed the number of colonies with significant differences. MIC in this study was at 5% concentration and MBC was at 20% concentration.. Conclusion: Ulin bark extract with 40% concentration has an absorbance value equivalent to 0.2% chlorhexidine and 20% concentration has a bactericidal effects equivalent to 0.2% chlorhexidine against the growth of P.gingivalis.Keywords:Antibacterial, Dilution method,Porphyromonas gingivalis, Ulin Bark Extract.

2021 ◽  
Vol 6 (1) ◽  
pp. 72
Author(s):  
Lailatul Qomariyah ◽  
Fransiska Uli Arta Panjaitan

ABSTRACTBackground: Chronic periodontitis is a periodontal disease with 80% of all cases of periodontitis. The major causes are the accumulation of plaque and bacteria. The dominant bacteria in chronic periodontitis is Porphyromonas gingivalis. Treatment of chronic periodontitis can be done by scaling and root planing and supporting therapy by using mouthwash such as Chlorhexidine gluconate 0.2% which is the gold standard in the treatment of periodontal disease. Chlorhexidine gluconate 0.2% has disadvantages so that nowadays research on herbal plants is being done to find alternative medicines that are more effective. Ramania (Bouea machropylla Griffith) leaf contains flavonoids that have antibacterial properties. Objective: To analyze the antibacterial effectivity of the flavonoid fraction of Ramania leaf extract against Porphyromonas gingivalis that causes chronic periodontitis. Method: True experimental study and post-test with control group design consisting of 5 treatment groups, namely flavonoid fraction of ramania leaf extract with concentrations of 0.1%, 0.3%, and 0.5%, chlorhexidine gluconate 0.2% as a control positive and aquadest as a negative control. Each group was repeated 6 times. Antibacterial tests using the dilution method with inhibitory rates calculated using a UV-Vis spectrophotometer and killing rates were calculated using a Colony Counter. Results: The average difference in absorbance values obtained inhibitory rates at concentrations of 0.1%, 0.3%, and 0.5%. One Way Anova Test showed a significance value of 0,000 (p < 0.05). The average number of colonies after 24 hours incubation showed the results of a kill rates in the concentration group of 0.3%, 0.5%, and positive control. The Kruskal Wallis test showed a significance value of 0,000 (p < 0.05). Conclusion: The minimum inhibitory concentration (MIC) was obtained at a concentration of 0.1% and the minimum bactericidal concentration (MBC) was obtained at 0.3% concentration.Keywords: Flavonoid Fraction, MBC, MIC, Porphyromonas Gingivalis, Ramania Leaf Extract.


2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.


2021 ◽  
Vol 9 (1) ◽  
pp. 19-28
Author(s):  
Monalisa Monalisa ◽  
Erly Erly ◽  
Aria Fransiska

Chronic periodontitis is the most common type of periodontitis. The main cause of chronic periodontitis is bacterial colonization of plaque. Porphyromonas gingivalis is the main pathogen and has the highest prevalence of chronic periodontitis. Bay leaf extract (Syzygium polyanthum wight) is believed to have the pharmacological effect that can be used as antibacterial. The purpose of this research was to determine the inhibitory of bay leaf extract on the growth of Porphyromonas gingivalis in vitro. The method of this research was experimental laboratories with posttest only control group design. There were 30 samples divided into 6 groups, namely bay leaf extract concentration of 2.5%, 5%, 10%, 20%, 40% and Dimethyl Sulfoxide (DMSO) as a control. Inhibitory test using the Kirby-Bauer method with a paper disc on Mueller Hinton medium agar. Inhibition zone that created around paper disc was measured with the sliding caliper. The results were analyzed with One Way Anova and Post Hoc LSD test. The results of this research showed that the mean of bay leaf extract 40% had the largest inhibition zone that was 7,6 mm and the lowest formed by bay leaf extract 2,5% that was 1,94 mm. One Way Anova test showed that there was a significant difference among the group (p<0,05). The conclusion of this research there was significant difference inhibition among 2,5%, 5%, 10%, 20%, and 40% concentration of bay leaf extract on the growth of Porphyromonas gingivalis in vitro. The higher concentration of bay leaf extract resulting the greater inhibition zone.


2021 ◽  
Vol 6 (2) ◽  
pp. 147
Author(s):  
Lailatul Qomariyah ◽  
Fransiska Uli Arta Panjaitan ◽  
Rosihan Adhani

Background: Chronic periodontitis is a periodontal disease with 80% of all cases of periodontitis. The major causes are the accumulation of plaque and bacteria. The dominant bacteria in chronic periodontitis is Porphyromonas gingivalis. Treatment of chronic periodontitis can be done by scaling and root planing and supporting therapy by using mouthwash such as Chlorhexidine gluconate 0.2% which is the gold standard in the treatment of periodontal disease. Chlorhexidine gluconate 0.2% has disadvantages so that nowadays research on herbal plants is being done to find alternative medicines that are more effective. Ramania (Bouea machropylla Griffith) leaf contains flavonoids that have antibacterial properties. Objective: To analyze the antibacterial effectivity of the flavonoid fraction of Ramania leaf extract against Porphyromonas gingivalis that causes chronic periodontitis. Method: True experimental study and post-test with control group design consisting of 5 treatment groups, namely flavonoid fraction of ramania leaf extract with concentrations of 0.1%, 0.3%, and 0.5%, chlorhexidine gluconate 0.2% as a control positive and aquadest as a negative control. Each group was repeated 6 times. Antibacterial tests using the dilution method with inhibitory rates calculated using a UV-Vis spectrophotometer and killing rates were calculated using a Colony Counter. Results: The average difference in absorbance values obtained inhibitory rates at concentrations of 0.1%, 0.3%, and 0.5%. One Way Anova Test showed a significance value of 0,000 (p < 0.05). The average number of colonies after 24 hours incubation showed the results of a kill rates in the concentration group of 0.3%, 0.5%, and positive control. The Kruskal Wallis test showed a significance value of 0,000 (p < 0.05). Conclusion: The minimum inhibitory concentration (MIC) was obtained at a concentration of 0.1% and the minimum bactericidal concentration (MBC) was obtained at 0.3% concentration. Keywords: Flavonoid Fraction, MIC, MBC, Porphyromonas Gingivalis, Ramania Leaf Extract.


2021 ◽  
Vol 7 (1) ◽  
pp. 1-8
Author(s):  
Muhammad Dzul Fikri ◽  
Oce Wiriawan ◽  
Achmad Widodo

Abstrak Penelitian ini dilatarbelakangi oleh penurunan prestasi atlet futsal SMA Antartika Sidoarjo. Penelitian ini bertujuan mengkaji pengaruh latihan Ladder Drill dengan metode latihan ascending dan metode latihan descending terhadap peningkatan kecepatan dan kelincahan pada siswa ekstrakurikuler futsal SMA Antartika Sidoarjo. Jenis penelitian dalam penelitian ini adalah kuantitatif dengan metode quasi-experiment. Desain yang digunakan dalam penelitian adalah Pretest-Posttest Control Group Design yang dilaksanakan dalam 24 kali pertemuan dalam 8 minggu. Instrumen tes kecepatan menggunakan lari 40 meter, sedangkan instrumen kelincahan menggunakan shuttle run test. Analisis data yang digunakan untuk mengolah hasil penelitian menggunakan uji-t, MANOVA, dan Post Hoc dengan uji Scheffe. Hasil penelitian menunjukkan peningkatan kecepatan dan kelincahan yang signifikan dari metode ascending dan descending. Sedangkan analisis post hoc uji Scheffe tidak terdapat perbedaan yang signifikan antara kelompok ascending dan kelompok descending terhadap kecepatan dan kelincahan. Hasil penelitian ini menyimpulkan bahwa kedua metode ascending dan descending, dapat meningkatkan kecepatan dan kelincahan sama baiknya terhadap atlet futsal SMA Antartika Sidoarjo. Abstract This research was motivated by a decrease in the achievement of futsal athletes at SMA Antartika Sidoarjo. This study aims to examine the effect of ladder drill training with ascending training methods and descending training methods on increasing speed and agility in futsal extracurricular students at SMA Antartika Sidoarjo. The type of research in this research is quantitative with a quasi-experimental method. The design used in the study was the Pretest-Posttest Control Group Design which was carried out in 24 meetings in 8 weeks. The speed test instrument uses 40 meters running, while the agility instrument uses the shuttle run test. Analysis of the data used to process the research results using the t-test, MANOVA, and Post Hoc with the Scheffe test. The results showed a significant increase in speed and agility from the ascending and descending methods. While the post hoc analysis of the Scheffe test, there was no significant difference between the ascending and descending groups in terms of speed and agility. The results of this study concluded that both ascending and descending methods can improve speed and agility as well as futsal athletes at SMA Antartika Sidoarjo.


2018 ◽  
Vol 6 (3) ◽  
pp. 461
Author(s):  
Mahda Febriyanti Eka Pertiwi Putri ◽  
Murtaqib Murtaqib ◽  
Mulia Hakam

During the quality of sleep the elderly is good, some changes that will occur to the elderly arenormal as well, but if not, it will cause health problems. This research aims to analyze theeffect of jasmine aromatherapy relaxation on sleeping quality to the elderly at elderlyassociation in Jenggawah Jember. This research used quasy experimental design methodwith pre-post test and control group design. Sleeping quality was measured using a PSQIquestionnaire and interventions which were given for 28 days. The result of dependent t testshowed no significant difference on the average score of control group (p value = 1,000).While in the intervention group there was a decrease in the mean score of PSQI after beinggiven jasmine aromatherapy relaxation, so that there was a significant difference in sleepingquality of the intervention group (p value = 0,001). Independent t tests showed significantdifferences between the control group and the intervention group after jasmine aromatherapyrelaxation (p value = 0,001). Overall, jasmine aromatherapy relaxation can improve sleepingquality in the elderly, so that jasmine aromatherapy relaxation may be used as an alternativenon-pharmacological treatment which has no adverse side effects compared to the use oflong-term sleeping pills that would give negative side effects to its users. Keywords: aromatherapy, sleeping quality, elderly.


2020 ◽  
Vol 5 (1) ◽  
pp. 53 ◽  
Author(s):  
Rahma Dania A.J ◽  
Deby Kania Tri Putri ◽  
Irham Taufiqurrahman

Background: Periodontal disease is one of the dental and oral diseases that is often found in people in Indonesia. RISKESDAS (2018) stated that the prevalence of people who have dental and oral health problems in Indonesia is 57.6%. The prevalence of oral and dental problems in South Kalimantan is 60%. Periodontal disease is damage to the supporting tissues of the teeth caused by specific microorganisms such as Porphyromonas gingivalis which are gram negative bacteria. The growth of these bacteria can be inhibited and killed by chitosan from haruan fish scales. Chitosan is a natural product of chitin, chitosan is the basic ingredient of fish scales. Chitosan has advantages and more safety levels, because it has an active group that will bind to microbes so chitosan can inhibit and kill microbial growth. Objective: to analyze the antibacterial activity of chitosan scales (Channa striata) on the growth of Porphyromonas gingivalis. Methods: This research used true experimental with randomized pre and post test with only control group design with eight treatments. Chitosan of haruan fish scales with liquid dilution method to obtain MIC and solid dilution test to obtain MBC. Results: The results showed that Minimum Inhibitory Concentration (MIC) of chitosan haruan scales was found at a concentration of 2.5% characterized by a decrease in absorbance value of 0.092 and Minimum Bactericidal Concentration found at a concentration of 20% characterized by the absence of Porphyromonas gingivalis. Conclusion: There is an inhibitory activity and a bactericidal activity from chitosan of haruan fish scales on Porphyromonas gingivalis.


2020 ◽  
Vol 13 (01) ◽  
pp. 26-33
Author(s):  
Gempita Cahya aulia tambunan ◽  
Aparna Dutt ◽  
Sayra Nadhifa ◽  
Firdha Amelia ◽  
Ermi Girsang

There are various potential natural anti-diabetic drugs; one of them is lime peel or Citrus amblycarpa. This study was aimed to explore the anti-diabetic activity and phytochemical content of lime peels. This study was an experimental study that used the post-test only control group design. The lime peels that were collected from the Berastagi fruit market in Medan, North Sumatera were extracted using 70% ethanol by maceration methods. The phytochemical screening identified the presence of phenolic, steroid/triterpenoid, terpenoid, saponin, flavonoid, tannin, and alkaloid. Meanwhile, the anti-diabetic activity of lime peels was evaluate using the α-glucosidase enzyme that was gotten from Saccharomyces cerevisiae by α-glucosidase enzyme inhibition methods. Percent of inhibition was express as Mean ± SD and analyzed by One Way ANOVA, Tukey HSD Post Hoc Test, and followed by linear regression. The result of this study showed that there is a significant difference in percentage inhibition α-glucosidase enzyme in each concentration, and it had an IC50 Value amount of 125.93 ± 9.14 µg/mL. The phytochemical content of the lime peels was flavonoid, phenol, steroid/triterpenoid, and alkaloid. Hence, the lime peel has anti-diabetic activity by inhibition of the α-glucosidase enzyme.


Author(s):  
Yusnaini Yusnaini

According to the WHO 40% of maternal deaths in developing countries related to anemia in pregnancy and is most commonly caused by iron deficiency and acute bleeding, even less so the two interact. This study aims to determine the effect of doses of extracts of Guava (Psidium Guajava. L) and Tablet Fe to changes in hemoglobin levels in mice (Mus musculus). Type True Experimental studies in laboratory design nonrandomized design Pretest-Posttest Control Group Design. Samples were mice (Mus musculus) as many as 20 birds were divided into four groups with each group number as many as five tails. Group A was given a tablet Fe as much as 0.126 mg / kg / day, group B was given a tablet Fe as much as 0.126 mg / KgBW / day coupled with extracts of Guava as much as 10 mg / kg / day, group C was given tablets Fe as much as 0.126 mg / kg / day coupled with guava extract as much as 20 mg / kg / day, group D was given tablets as much as 0.126 mg Fe / KgBW / day plus guava extract as much as 30 mg / kg / day. The data collection was conducted from June 5 s / d July 20, 2015. Data were analyzed using Paired T-Test continued with Test and One Way ANOVA Post Hoc Test namely LSD test. Results showed that there was no significant difference in hemoglobin levels between the groups in which the value of P = 0634> 0.05.. Based on the results of this study concluded that there was no effect of dose of extract of guava (Psidium Guajava. L) and Tablet Fe to changes in hemoglobin levels in mice (Mus musculus), but the effect on the number of erythrocytes of mice (Mus musculus)


2018 ◽  
Vol 4 (1) ◽  
pp. 30
Author(s):  
Teuku Fadhli

Rendahnya tingkat pemahaman karier siswa dipengaruhi oleh minimnya informasi karier mengenai pekerjaan dan pendidikan untuk mendukung sebuah pekerjaan dimasa akan datang. Tujuan dari penelitian ini adalah untuk menguji keefektifan layanan informasi karier dengan teknik live modeling dan symbolic modeling untuk meningkatkan pemahaman karier siwa. Metode penelitian yang digunakan adalah eksperimen, dengan desain Non Equivalent Group Pretest-Postest Control Group, subyek penelitian sebanyak 96 orang siswa kelas IX SMP Teuku Umar yang dibagi menjadi tiga kelompok, yakni kelompok A, B dan Kontrol. Hasil analisis data One way ANOVA menunjukkan bahwa teknik live modeling dan symbolic modeling (p<0.01; F=108.015). Sedangkan pengujian post hoc test menunjukkan (MD=-1.81; p=0.97>0.05) yang berarti teknik live modeling dan symbolic modeling tidak terdapat perbedaan rata-rata signifikan, untuk teknik live modeling dan kelompok kontrol dengan(MD= -59.28; p<0.01), dan untuk teknik symbolic modeling dan kelompok kontrol dengan (MD=-6109; p<0.01), yang berarti teknik live modeling dan symbolic modeling rata-rata efektif untuk meningkatkan pemahaman karier siswa siswa SMP Teuku Umar Semarang.__________________________________________________________ The students’ low understanding in career is influenced by alack of information about career and education in which those aspects are needed to realize a certain job in the future. This study aims to examine the effectiveness of career information services with live and symbolic modeling techniques to improve students' career understanding. The research method used is experimental with operating Non Equivalent Group Pretest-Postest Control Group design, 96 students of 9th grade of SMP Teuku Umar shared in 3 classes were employed as subject, namely is A, B and Control group. The one-way data analysis shows the technique of live  and symbolic modeling whereas (p <0.01; F=108.015). While the post hoc test shows (MD=-1.81;p=0.97>0.05), it means there is no significant difference both live and symbolic modeling techniques. In addition, it is also known that live modeling and control group whereas (MD=-59.28; p<0.01 ), symbolic modeling and control group whereas (MD=-6109; p<0.01). Therefore, it could be concluded that live and symbolic modeling technique is effective to improve students’ career understanding at SMP Teuku Umar Semarang.


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