scholarly journals ISOLATION OF CLOVE ESSENTIAL OIL BY FERMENTATION PROCESS

Konversi ◽  
2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Khurotul Ainiyah ◽  
Fita Andriyani ◽  
Soemargono Soemargono ◽  
Nove Kartika Erliyanti
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Steam Distillation ◽  
Chemical Compounds ◽  
Rhizopus Oligosporus ◽  
Fermentation Time ◽  
Fermentation Method ◽  
Isolation Methods ◽  
Leaf Essential Oil ◽  
Clove Essential Oil

Clove essential oil is processed product of clove leaves which is used as perfumes and medicines. In general, the method used to extract clove leaf essential oil is the steam distillation method. This method has not maximized the yield essential oils produced because a lot of essential oils are still trapped in plant tissues. Modification of the essential oil isolation methods to produce more yields needs to be done, one of which is the fermentation method with the help Rhizopus oligosporus before the distillation process is carried out. The fermentation method is able to destroy the oil tissues on the leaves using enzymes produced by rhizopus oligosporus. The aims of this study were to isolate the essential oil clove leaves and look for the effect of the mass and fermentation time on the yield and content of clove leaf essential oil compounds produced. The results showed that the isolation  essential oils using fermentation methods was successfully carried out, the mass and fermentation time had a significant influence on the yield and content of essential oil chemical compounds. The best result was obtained at a mass of 35 grams with a fermentation time of 36 hours with a yield 0.32% and the content eugenol, caryopillene compounds obtained respectively at 93.43% and 2.28%.

scholarly journals Chemical Compositions, Mosquito Larvicidal and Antimicrobial Activities of Essential Oils from Five Species of Cinnamomum Growing Wild in North Central Vietnam

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Antimicrobial Agents ◽  
Chemical Compositions ◽  
North Central ◽  
Mosquito Larvicidal Activity ◽  
Central Vietnam ◽  
Leaf Essential Oil

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.

Enzyme‐assisted extraction of essential oils from Syzygium aromaticum

2011 ◽  
Vol 1 (6) ◽  
pp. 248-254
Author(s):  
R. Amudan ◽  
D. V. Kamat ◽  
S. D. Kamat
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Steam Distillation ◽  
Antibacterial Effect ◽  
Chemical Extraction ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Zone Size ◽  
Structure Quality ◽  
Assisted Extraction

The essential oil of clove ( Syzygium aromaticum) holds an important positionamongst widely used essential oils. A typical steam distillation processfor the extraction of clove oil provides a 10.1% yield. Recent studies involvedthe use of enzymes such as pectinase, amylase, lignocellulase, and cellulaseon the powder of clove buds, prior to extraction. The traditional methods ofphysical and chemical extraction are effective but may affect the structure,quality and yield of the phytochemicals extracted. In the current study,hence, enzymes specific for action on the cell wall have been used in the pretreatment prior to extraction, to enhance the quality and yield of the phytochemicalsextracted. The results indicated that all the enzymes, gave morethan 50% higher yield than control in terms of weight of extracted essentialoil. A mixture of the enzymes gave the highest yield of 17.82%. Gas chromatographyresults indicated that the essential oil extracted using amylase hada maximum eugenol content of 70%, in comparison with the eugenol content(62–68%) in the essential oils extracted using the rest of the enzymes.Antibacterial activity of all the extracts was studied on methicillin â€resistantStaphylococcus aureus  (MRSA). The essential oil extracted by using amylaseinhibitedMRSA, showed a zone size of 40 mm, whereas the essential oil extractedby using lignocellulase showed a zone size of 45 mm. The gas chromatogramindicated the maximum number of peaks in this extract, whichcould be producing a combined antibacterial effect on the organism. Thespecific gravity values of the essential oil extracted using lignocellulase andamylase was 1.051 and 1.062, respectively, whereas the control had a specificgravity of 1.015.

scholarly journals Chemical composition and content of essential oil from the bud of cultivated Turkish clove (Syzygium aromaticum L.)

BioResources ◽  
2007 ◽  
Vol 2 (2) ◽  
pp. 265-269 ◽  
Author(s):  
M. Hakki Alma ◽  
Murat Ertaş ◽  
Siegfrie Nitz ◽  
Hubert Kollmannsberger
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Mediterranean Region ◽  
Steam Distillation ◽  
Syzygium Aromaticum ◽  
Distillation Method ◽  
Eugenyl Acetate ◽  
Clove Bud Oil ◽  
Oil Company

In this study, clove bud oil, which was cultivated in the Mediterranean region of Turkey, was provided from a private essential oil company in Turkey. Essential oil from clove (Syzygium aromaticum L.) was obtained from steam-distillation method, and its chemical composition was analyzed by GC and GC-MS. The results showed that the essential oils mainly contained about 87.00% eugenol, 8.01% eugenyl acetate and 3.56% β-Caryophyllene. The chemical composition of the Turkish clove bud oil was comparable to those of trees naturally grown in their native regions.

scholarly journals Chemical Composition of Cinnamomum verum Leaf and Flower Essential Oils and Analysis of Their Antibacterial, Insecticidal, and Larvicidal Properties

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6303
Author(s):  
Arunaksharan Narayanankutty ◽  
Krishnaprasad Kunnath ◽  
Ahmed Alfarhan ◽  
Rajakrishnan Rajagopal ◽  
Varsha Ramesh
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Callosobruchus Maculatus ◽  
Sitophilus Oryzae ◽  
Microbial Pathogens ◽  
Traditional Medicines ◽  
Significant Toxicity ◽  
Leaf Essential Oil ◽  
Different Parts

Cinnamomum verum is widely used in traditional medicines, and the different parts of the plant, such as bark, leaves, and flowers, are used for essential oil production. The present study compared the chemical composition of the essential oil of C. verum extracted from the leaves and flowers. In addition, efficacy of these essential oils against the two common pests Sitophilus oryzae and Callosobruchus maculatus was also evaluated. The results indicated the presence of cinnamaldehyde, eugenol, caryophyllene, and linalool in these essential oils, however, at different concentrations. The leaf essential oil was found to be 10–20% more effective as a fumigant against both the pests. Likewise, the leaf essential oil found to repel these pests even at lower concentrations than that of flower essential oil of C. verum. Besides, these essential oils were also effective in controlling the growth of various gram positive and gram negative microbial pathogens and possibly a safeguard for human health. On contrary, both the essential oils were found to be safe for the application on grains, as indicated by their germination potentials. It was also observed that these essential oils do not cause any significant toxicity to guppy fishes, thus confirming their ecological safety for use as a biopesticide.

scholarly journals Transcriptomic Analysis of Fertilization Effects on the Biosynthesis of Sesquiterpenes in Essential Oils from Phoebe Bournei Twigs and Leaves

2021 ◽  
Author(s):  
Li Liu ◽  
Gongxiu He ◽  
Xu Wang ◽  
Dangquan Zhang
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Plant Biomass ◽  
Raw Material ◽  
Compound Fertilizer ◽  
Expression Of Genes ◽  
Leaf Essential Oil ◽  
Main Components ◽  
Fertilization Effects ◽  
Phoebe Bournei

Abstract Background: Phoebe bournei is a potential medicinal plant whose essential oil (EO) from leaves has potential inhibitory activities against some bacterium, tumor, and has a certain potential for hypoglycemic activity. Fertilization is a common and effective method to increase plant biomass, which can increase the raw material of essential oil, but has a certain impact on the composition and biological activity of plant essential oil. Results: The main components are sesquiterpenes in the essential oils from leaves and twigs. The yield of the essential oils and the content of their main components can be modulated by compost and compound fertilizer, to different degrees, and minor differences were registered among the categories of the components in essential oils. However, changes were strongly mirrored in some main components of essential oils. The content of the primary (+) - calarene in the leaf EO were strongly increased by compost, but the opposite happened by compound fertilizer. On the contrary, the effect of compound fertilizer was more significant on the main components of twig essential oil than compost. The transcriptome sequencing results of P. bournei showed that the total number of DEGs in twigs and leaves treated with compost were significantly more than that with compound fertilizer. No change was found in the expression of genes regulating principal components. However, the expression of several key genes regulating the upstream substrates for the synthesis of the sesquiterpenes was significantly changed: the expression of two key speed limiting enzymes genes (DXS and HMGR) and two important branch-point enzyme genes (FPPS and GGPPS) was significantly down regulated, while the expression of gene (HMGS) was significantly up-regulated.Conclusion: The expression levels of genes (DXS2, HMGR, FPPS and GGPPS) were significantly down regulated in leaves treated with compost, resulting in the changes of the yield and main components of the leaf essential oil. The effect of compost was more significant on the synthesis of the essential oil from P. bournei leaves than that of compound fertilizer.

Anti-Proliferative Activity of Piper trioicum Leaf Essential Oil Based on Phytoconstituent Analysis, Molecular Docking and In Silico ADMET Approaches

2021 ◽  
Vol 25 ◽  
Author(s):  
Sudipta Jena ◽  
Asit Ray ◽  
Ambika Sahoo ◽  
Prabhat Kumar Das ◽  
Pradeep Kumar Kamila ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Cell Lines ◽  
In Silico ◽  
Proliferative Activity ◽  
In Silico Admet ◽  
Leaf Essential Oil ◽  
Ht 29

Background: The essential oils isolated from several medicinal plants are reported to have anticancer activities. Both the essential oil and extracts of many Piper species (Piperaceae) possess potential cytotoxic effect against cancer cell lines and are being used in traditional system of medicine for the treatment of cancer. There is a need to evaluate and validate the anticancer properties of essential oils extracted from other wild species of Piper. Objective: The current research was undertaken to determine the chemical composition and investigate the anti-proliferative activity of wild growing Piper trioicum leaf essential oil. The selected five major constituents were subjected to molecular docking to identify possible modes of binding against serine/threonine-protein kinase (MST3) protein Methods: The essential oil of leaf of P. trioicum was extracted by hydro distillation method and its chemical composition was carried out by GC-FID and GC-MS. The anti-proliferative activity of the essential oil was evaluated by MTT assay against normal (3T3-L1) and various cancer (HCT 116, HT-29, PC-3 and HepG2) cell lines. Molecular docking analysis was performed using AutoDock 4.2 software. The pharmacokinetic and pharmacodynamic properties of the major constituents were determined using absorption, distribution, metabolization, excretion and toxicity (ADMET) analysis. Results: The GC-MS analysis revealed the identification of 45 constituents with δ-cadinene (19.57%), germacrene-D (8.54%), β-caryophyllene (6.84%), 1-epi-cubenol (4.83%) and α-pinene (4.52%) were found to be predominant constituents in the leaf essential oil of P. trioicum. The highest cytotoxicity of essential oil was observed against HT-29 cells (IC50 value of 33.14 µg/ml). 1-epi-cubenol and δ-Cadinene exhibited low binding energy values of -6.25 and -5.92 kcal/mol, respectively. For prediction of in silico pharmacokinetic and druglike properties of the major compounds, ADMET prediction tool was also used, the results of which came within the ideal range. Conclusion: The present findings demonstrated that P. trioicum essential oil possesses significant anti-proliferative activity and could be effective against cancer treatment.

scholarly journals Composition of Essential Oils from Leaves and Flowers of Stachys Germanica Subsp. Salviifolia (Ten.) Gams (Labiatae) and Related Secretory Structures

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Claudia Giuliani ◽  
Roberto Maria Pellegrino ◽  
Bruno Tirillini ◽  
Laura Maleci Bini
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Glandular Trichomes ◽  
Secretory Structures ◽  
Epidermal Surface ◽  
Germacrene D ◽  
Different Types ◽  
Leaf Oil ◽  
Leaf Essential Oil ◽  
First Time

The essential oils from both leaves (L) and flowers (F) of Stachys germanica subsp. salviifolia (Ten.) Gams (Labiatae/Lamiaceae) from Italy were analyzed by GC-MS for the first time. The distribution, morphology and histochemistry of the different types of glandular trichomes present on the epidermal surface were also determined. Twenty-nine constituents, representing 89.4% of the total leaf oil, and forty-one compounds, forming 97.8% of the total flower oil, were identified. Germacrene D (39.4%), phytol (10.2%), β-bourbonene (3.5%) and β-ylangene (3.3%) were recognized as the main constituents of the leaf essential oil, while limonene (24.1%), β-pinene (18.7%), germacrene D (12.8%) and ( E)-nerolidol (6.6%) were the main compounds of the flower essential oil.

scholarly journals Activity of Schinus Areira (Anacardiaceae) Essential Oils against the Grain Storage Pest Tribolium castaneum

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Lilian R. Descamps ◽  
Carolina Sánchez Chopa ◽  
Adriana A. Ferrero
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Feeding Deterrent ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Nutritional Index ◽  
Leaf Essential Oil ◽  
Storage Pest ◽  
Coleoptera Tenebrionidae

Essential oils extracted from leaves and fruits of Schinus areira (Anacardiaceae) were tested for their repellent, toxic and feeding deterrent properties against Tribolium castaneum (Coleoptera: Tenebrionidae) larvae and adults. A topical application assay was employed for the contact toxicity study and filter paper impregnation for the fumigant assay. A treated diet was also used to evaluate the repellent activity and a flour disk bioassay for the feeding deterrent action and nutritional index alteration. The essential oil of the leaves contained mainly monoterpenoids, with α-phellandrene, 3-carene and camphene predominant, whereas that from the fruits contained mainly α-phellandrene, 3-carene and β-myrcene. The leaf essential oil showed repellent effects, whereas that from the fruit was an attractant. Both oils produced mortality against larvae in topical and fumigant bioassays, but fumigant toxicity was not found against adults. Moreover, both essential oils produced some alterations in nutritional index. These results show that the essential oils from S. areira could be applicable to the management of populations of Tribolium castaneum.

scholarly journals CHEMICAL COMPOUNDS AND ANTIOXIDANT CONTENTS OF CLOVES LEAVES ESSENTIAL OIL

2020 ◽  
Vol 7 (1) ◽  
pp. 37-44
Author(s):  
Muhammad Tahir ◽  
Siti Chuzaemi ◽  
Eko Widodo ◽  
Hafsah Hafsah
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Chemical Compounds ◽  
Antioxidant Content ◽  
Ic50 Values ◽  
Anti Fungal ◽  
Central Sulawesi

Essential oils from the distillation of clove leaves could act as anti-bacterial and anti-fungal agentsforeither food or feed. The purpose of this study was to identify chemical compounds and antioxidant content of clove leaves essential oils. The material used was clove leaves essential oil obtained by soxlet extraction. Clove leaves were taken from trees of over 10 years old in Palu, Central Sulawesi. Chemical compounds of the sample were analyzed by GC-MS method. Each peak that appeared on the chromatogram was identified by comparing to the standard. While the antioxidant analysis was carried out by using the DPPH (IC50) of spectrophotometry method. The results showed that the total essential oils content of clove leaves was 3,957% and there were 6 chemical compounds identified, i.e. caryophyllen (0,96%); phenol, 2-methoxy (guaiacol) (4,16%); phenol,2-methoxy-4-methyl (9.79%); phenol, 4-ethyl-2-methoxy (1.5%); phenol 2-methoxy-4-propyl (3.87%); phenol, 2-methoxy-4-(2-prophenyl) andEugenol (79.72%). While the results of antioxidant identification based on DPPH and IC50 values before purification were 0.597 and 74.358, and the respective values after purification were 0.597 and 42.255. In conclusion, the antioxidant content of clove leaves essential oils after purification is higher than before purification and it contains 3.957% essential oil.

scholarly journals Syzygium aromaticum Essential Oil to the Control of Tenuipalpus heveae

2019 ◽  
Vol 11 (8) ◽  
pp. 295
Author(s):  
Thalia Mayara Pereira de Melo ◽  
Eliamara Marques da Silva ◽  
Abimael Gomes da Silva ◽  
Gustavo Haralampidou da Costa Vieira ◽  
Beatriz Garcia Lopes
Keyword(s):  
Regression Analysis ◽  
Essential Oil ◽  
Essential Oils ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Adult Females ◽  
Clove Essential Oil ◽  
Petri Dishes ◽  
Adult Mite ◽  
Mite Mortality

The objective was to determine the effect of essential oils on the mortality and repellency of the Tenuipalpus heveae Baker mite (Acari, Tenuipalpidae). Leaf discs of rubber trees were deposited in Petri dishes containing a moistened cotton layer. Posteriorly, the essential oils were diluted to formulate solutions, which were sprayed onto the discs. The experimental design was completely randomized, in a 3 × 4 factorial design: neem, cedar and clove oils at doses 0, 10 μL, 50 μL and 100 μL, with five replicates, with ten adult females being considered per replicate. The exposure to the effect of essential oil was evaluated after 24, 48, 72 and 96 hours for the number of females dead and females in the cotton. The data were submitted to analysis of variance, the values were transformed into (x + 0.5)1/2, and the means were grouped by the Scott-Knot test at 5% probability for the treatments, the doses were submitted to regression analysis. From the 48 hours of evaluation, clove oil showed greater potential for adult mite mortality, being 1.38 in 48 hours, 1.40 in 72 hours and 1.50 in 96 hours. The dose of 100 μL presented a greater potential for the neem and clove oils. The clove essential oil has potential in the control of Tenuipalpus heveae.

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