Phenotypic Correlations among the Negative Energy Balance Indicators and Milk Yield in High Yielding Murrah Buffaloes

Crossbreeding in dairy cattle has been used to improve functional traits, milk composition, and efficiency of Holstein herds. The objective of the study was to compare indicators of the metabolic energy balance, nonesterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), glucose, body condition score (BCS) back fat thickness (BFT), as well as milk yield and milk composition of Holstein and Simmental cows, and their crosses from the prepartum period until the 100th day of lactation at the Livestock Center of the Ludwig Maximilians University (Munich, Germany). In total, 164 cows formed five genetic groups according to their theoretic proportion of Holstein and Simmental genes as follows: Holstein (100% Holstein; n = 9), R1-Hol (51–99% Holstein; n = 30), first generation (F1) crossbreds (50% Holstein, 50% Simmental; n = 17), R1-Sim (1–49% Holstein; n = 81) and Simmental (100% Simmental; n = 27). The study took place between April 2018 and August 2019. BCS, BFT blood parameters, such as BHBA, glucose, and NEFA were recorded weekly. A mixed model analysis with fixed effects breed, week (relative to calving), the interaction of breed and week, parity, calving year, calving season, milking season, and the repeated measure effect of cow was used. BCS increased with the Simmental proportion. All genetic groups lost BCS and BFT after calving. Simmental cows showed lower NEFA values. BHBA and glucose did not differ among genetic groups, but they differed depending on the week relative to calving. Simmental and R1-Sim cows showed a smaller effect than the other genetic groups regarding changes in body weight, BCS, or back fat thickness after a period of a negative energy balance after calving. There was no significant difference for milk yield among genetic groups, although Simmental cows showed a lower milk yield after the third week after calving. Generally, Simmental and R1-Simmental cows seemed to deal better with a negative energy balance after calving than purebred Holstein and the other crossbred lines. Based on a positive heterosis effect of 10.06% for energy corrected milk (ECM), the F1, however, was the most efficient crossbred line.

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Effect of Rumen Protected Methionine and Choline on Blood Biochemical Metabolites, Milk Yield and its Composition during Transition Period in Surti Buffaloes

Indian Journal of Animal Research ◽

10.18805/ijar.b-4681 ◽

2021 ◽

Author(s):

S.D. Rathwa ◽

S.S. Chaudhary ◽

V.K. Singh ◽

S.B. Patel ◽

T.D. Manat

Keyword(s):

Energy Balance ◽

Milk Yield ◽

Milk Fat ◽

Transition Period ◽

Basal Diet ◽

Negative Energy ◽

Milk Quality ◽

Negative Energy Balance ◽

Blood Biochemical ◽

Liver Health

Background: Methionine and choline supplementation can aid in nutritionally managing transition Surti buffaloes. Present study has evaluated blood biochemical metabolites, milk yield and its changes in composition on supplementation of rumen protected methionine (RPM) and choline (RPC) in transition Surti buffaloes. Methods: Twenty-seven pregnant multiparous Surti buffaloes in three groups (n=9) from -15 d to 30 d postpartum received supplementation as: T1 (Control: basal diet), T2 (basal diet + RPM@10 gm/animal/day) and T3 (basal diet + RPM@10 gm/animal/day + RPC@ 50 gm/animal/day). Sample was collected at beginning, 1st, 3rd and 6th week for blood and at 1st, 3rd, 6th, 9th and 12th week postpartum for milk. Milk yield was recorded upto 100 days postpartum. Result: Postpartum TC, HDL and VLDL differed significantly (P less than 0.05) being highest in T3 and lowest in control (T1) whereas it was reverse for NEFA and BHBA. Supplemented groups had significantly lower TG levels at 1st and 3rd week postpartum. Milk fat upto 9th and SNF, protein, lactose, TAS, Ca, P and Mg upto 6th week were significantly (P less than 0.05) highest in T3, followed by T2 and T1. It was concluded that RPC along with RPM supplementation is more beneficial than only RPM supplementation in terms of enhancing liver health, reducing negative energy balance and improving milk quality.

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Metabolic evaluation of dairy cows submitted to three different strategies to decrease the effects of negative energy balance in early postpartum

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2011001300003 ◽

2011 ◽

Vol 31 (suppl 1) ◽

pp. 11-17 ◽

Cited By ~ 11

Author(s):

Alejandra M.B García ◽

Felipe C Cardoso ◽

Rómulo Campos ◽

Diego X Thedy ◽

Félix H.D González

Keyword(s):

Energy Balance ◽

Fatty Liver ◽

Dairy Cows ◽

Milk Yield ◽

Ketone Bodies ◽

Negative Energy ◽

Negative Energy Balance ◽

Early Lactation ◽

Blood Samples ◽

Subclinical Ketosis

In early lactation dairy cattle suffer metabolic alterations caused by negative energy balance, which predisposes to fatty liver and ketosis. The aim of this study was to evaluate the metabolic condition of high yielding dairy cows subjected to three treatments for preventing severe lipomobilization and ketosis in early lactation. Fifty four multiparous Holstein cows yielding >30 L/day were divided into four groups: control (CN= no treatment), glucose precursor (PG= propylene-glycol), hepatic protector (Mp= Mercepton®), and energy supplement with salts of linolenic and linoleic faty acids (Mg-E= Megalac-E®). Treatments were administrated randomly at moment of calving until 8 weeks postpartum. Blood samples were collected on days 1, 7, 14, 21, 28, 35, 42 and 49 postpartum. Body condition score (BCS) was evaluated at the same periods and milk yield was recorded at 2nd, 4th, 5th, 6th, 7th, and 8th weeks of lactation. Concentrations of non-esterified fatty acids (NEFA), albumin, AST, ß-hydroxybutyrate (BHBA), cholesterol, glucose, total protein, urea and triglycerides were analyzed in blood samples. Cut-off points for subclinical ketosis were defined when BHBA >1.4 mmol/L and NEFA >0.7 mmol/L. General occurrence of subclinical ketosis was 24% during the period. An ascendant curve of cholesterol and glucose was observed from the 1st to the 8th week of lactation, while any tendency was observed with BHBA and NEFA, although differences among treatments were detected (p<0.05). BCS decreased from a mean of 3.85 at 1st week to 2.53 at 8th week of lactation (p=0.001). Milk yield was higher in the Mg-E group compared with the other treatment groups (p<0.05) Compared with the CN group, the treatments with Mp and PG did not show significant differences in blood biochemistry and milk yield. Cows receiving PG and Mg-E showed higher values of BHBA and NEFA (P<0.05), indicating accentuated lipomobilization. Supplementation with Mg-E also resulted in significant higher concentrations of cholesterol, BHBA, urea, AST and lower values of glycemia. This performance may be explained by the highest milk yield observed with this treatment. Treatments with PG and Mp did not improve milk yield, compared with control cows, but did not show metabolic evidence of ketosis, fat mobilization or fatty liver. These results suggest that treatment with Mg-E improves milk production but induces a higher negative energy balance leading to moderated lipomobilization and ketone bodies production, increasing the risk of fatty liver.

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Reducing or eliminating the dry period of dairy cows

Australian Journal of Experimental Agriculture ◽

10.1071/ea05304 ◽

2006 ◽

Vol 46 (7) ◽

pp. 957 ◽

Cited By ~ 2

Author(s):

C. R. Stockdale

Keyword(s):

Energy Balance ◽

Dairy Cows ◽

Milk Yield ◽

Body Condition ◽

Post Partum ◽

Negative Energy ◽

Late Gestation ◽

Negative Energy Balance ◽

Dry Period ◽

Dairy Farmers

This review considers the research that has been conducted recently on reducing the length of the dry period of dairy cows, with particular emphasis on the effects of eliminating the dry period altogether. Milk yield in the subsequent lactation is reduced by up to 25%, but this loss is offset to some degree by the milk produced when cows would otherwise be dry. The lower subsequent milk yield in cows continuously milked is most likely to be a consequence of changes in the mammary gland during late gestation rather than insufficient feed or body condition to maintain milk synthesis. Shortening or eliminating the dry period may result in a lower incidence of metabolic problems post-partum, and a reduced negative energy balance in early lactation due to the maintenance of dietary intake while milk yields and body condition loss are reduced. The reductions in both body condition loss and negative energy balance may have a beneficial influence on reproductive performance. However, it is concluded that more research, particularly with cows that graze pasture during lactation, together with an economic appraisal, is needed before it could be recommended that Australian dairy farmers change their current dry period practices, particularly if continuous milking was to be considered.

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High serum free fatty acids and low leptin levels: Plausible metabolic indicators of negative energy balance in early lactating Murrah buffaloes

Journal of Cellular Physiology ◽

10.1002/jcp.28081 ◽

2019 ◽

Vol 234 (6) ◽

pp. 7725-7733 ◽

Cited By ~ 4

Author(s):

Naresh Golla ◽

Alka Chopra ◽

Sanjanna Boya ◽

Thota Venkata Chaitanya Kumar ◽

Suneel Kumar Onteru ◽

...

Keyword(s):

Fatty Acids ◽

Energy Balance ◽

Free Fatty Acids ◽

Negative Energy ◽

High Serum ◽

Negative Energy Balance ◽

Serum Free Fatty Acids ◽

Serum Free ◽

Murrah Buffaloes

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Negative energy balance during military training: the role of contextual limitations

Appetite ◽

10.1016/j.appet.2021.105263 ◽

2021 ◽

pp. 105263

Author(s):

Keyne Charlot

Keyword(s):

Energy Balance ◽

Military Training ◽

Negative Energy ◽

Negative Energy Balance

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Metabolic Adaptations to Dynamic Energy Requirements during Lactation and Pregnancy in Dairy Cows with Varying Proportions of Holstein and Simmental Breed

Proceedings ◽

10.3390/ieca2020-08833 ◽

2020 ◽

Vol 73 (1) ◽

pp. 9

Author(s):

Deise Aline Knob ◽

André Thaler Neto ◽

Helen Schweizer ◽

Anna Weigand ◽

Roberto Kappes ◽

...

Keyword(s):

Energy Balance ◽

Dairy Cows ◽

Fixed Effects ◽

Mixed Model ◽

Negative Energy ◽

Negative Energy Balance ◽

Repeated Measure ◽

Holstein Cows ◽

Mixed Model Analysis ◽

Genetic Groups

Depending on the breed or crossbreed line, cows have to cope with a more or less severe negative energy balance during the period of high milk yields in early lactation, which can be detected by beta-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFAs) in blood. Preventing cows from undergoing a severe negative energy balance by breeding and/or feeding measures is likely to be supported by the public and may help to improve the sustainability of milk production. The aim was to compare BHBA and NEFA concentrations in the blood of Holstein and Simmental cows and their crosses during the prepartum period until the end of lactation. In total, 164 cows formed five genetic groups according to their theoretic proportion of Holstein and Simmental genes as follows: Holstein (100% Holstein; n = 9), R1-Hol (51–99% Holstein; n = 30), F1 crossbreds (50% Holstein, 50% Simmental; n = 17), R1-Sim (1–49% Holstein; n = 81) and Simmental (100% Simmental; n = 27). NEFA and BHBA were evaluated once a week between April 2018 and August 2019. A mixed model analysis with fixed effects breed, week (relative to calving), the interaction of breed and week, parity, calving year, calving season, milking season, and the repeated measure effect on cows was used. Holstein cows had higher NEFAs (0.196 ± 0.013 mmol/L), and Simmental cows had the lowest NEFA concentrations (0.147 ± 0.008 mmol/L, p = 0.03). R1-Sim, F1 and R1-Hol cows had intermediate values (0.166 ± 0.005, 0.165 ± 0.010, 0.162 ± 0.008 mmol/L; respectively). The highest NEFA value was found in the first week after calving (0.49 ± 0.013 mmol/L). BHBA did not differ among genetic groups (p = 0.1007). There was, however, an interaction between the genetic group and week (p = 0.03). While Simmental, R1-Sim and F1 cows had the highest BHBA value, the second week after calving (0.92 ± 0.07 and 1.05 ± 0.04, and 1.10 ± 0.10 mmol/L, respectively), R1-Hol and Holstein cows showed the BHBA peak at the fourth week after calving (1.16 ± 0.07 and 1.36 ± 0.12 mmol/L, respectively). Unexpectedly, Holstein cows had a high BHBA peak again at week 34 after calving (1.68 ± 0.21 mmol/L). The genetic composition of the cows affects NEFA and BHBA. Simmental and R1-Sim cows mobilize fewer body reserves after calving. Therefore, dairy cows with higher degrees of Simmental origin might be more sustainable in comparison with Holstein genetics in the present study.

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The Capacity of Holstein-Friesian and Simmental Cows to Correct a Negative Energy Balance in Relation to Their Performance Parameters, Course of Lactation, and Selected Milk Components

Animals ◽

10.3390/ani11061674 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1674

Author(s):

Ilona Strączek ◽

Krzysztof Młynek ◽

Agata Danielewicz

Keyword(s):

Fatty Acid ◽

Energy Balance ◽

Dairy Cows ◽

Body Condition ◽

Body Condition Score ◽

Negative Energy ◽

Negative Energy Balance ◽

Holstein Friesian ◽

Condition Score ◽

Peak Lactation

A significant factor in improving the performance of dairy cows is their physiological ability to correct a negative energy balance (NEB). This study, using Simmental (SIM) and Holstein-Friesian (HF) cows, aimed to assess changes in NEB (non-esterified fatty acid; body condition score; and C16:0, C18:0, and C18:1) and its effect on the metabolic efficiency of the liver (β-hydroxybutyrate and urea). The effects of NEB on daily yield, production at peak lactation and its duration, and changes in selected milk components were assessed during complete lactation. Up to peak lactation, the loss of the body condition score was similar in both breeds. Subsequently, SIM cows more efficiently restored their BCS. HF cows reached peak lactation faster and with a higher milk yield, but they were less able to correct NEB. During lactation, their non-esterified fatty acid, β-hydroxybutyrate, C16:0, C18:0, C18:1, and urea levels were persistently higher, which may indicate less efficient liver function during NEB. The dynamics of NEB were linked to levels of leptin, which has anorectic effects. Its content was usually higher in HF cows and during intensive lactogenesis. An effective response to NEB may be exploited to improve the production and nutritional properties of milk. In the long term, it may extend dairy cows’ productive life and increase lifetime yield.

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Energy Balance and Energy Availability During a Selection Course for Belgian Paratroopers

Military Medicine ◽

10.1093/milmed/usab140 ◽

2021 ◽

Author(s):

Patrick Mullie ◽

Pieter Maes ◽

Laurens van Veelen ◽

Damien Van Tiggelen ◽

Peter Clarys

Keyword(s):

Physical Activity ◽

Energy Balance ◽

Energy Expenditure ◽

Metabolic Rate ◽

Energy Intake ◽

Rest Metabolic Rate ◽

Negative Energy ◽

Fat Free Mass ◽

Negative Energy Balance ◽

Energy Availability

ABSTRACT Introduction Adequate energy supply is a prerequisite for optimal performances and recovery. The aims of the present study were to estimate energy balance and energy availability during a selection course for Belgian paratroopers. Methods Energy expenditure by physical activity was measured with accelerometer (ActiGraph GT3X+, ActiGraph LLC, Pensacola, FL, USA) and rest metabolic rate in Cal.d−1 with Tinsley et al.’s equation based on fat-free mass = 25.9 × fat-free mass in kg + 284. Participants had only access to the French individual combat rations of 3,600 Cal.d−1, and body fat mass was measured with quadripolar impedance (Omron BF508, Omron, Osaka, Japan). Energy availability was calculated by the formula: ([energy intake in foods and beverages] − [energy expenditure physical activity])/kg FFM−1.d−1, with FFM = fat-free mass. Results Mean (SD) age of the 35 participants was 25.1 (4.18) years, and mean (SD) percentage fat mass was 12.0% (3.82). Mean (SD) total energy expenditure, i.e., the sum of rest metabolic rate, dietary-induced thermogenesis, and physical activity, was 5,262 Cal.d−1 (621.2), with percentile 25 at 4,791 Cal.d−1 and percentile 75 at 5,647 Cal.d−1, a difference of 856 Cal.d−1. Mean daily energy intake was 3,600 Cal.d−1, giving a negative energy balance of 1,662 (621.2) Cal.d−1. Mean energy availability was 9.3 Cal.kg FFM−1.d−1. Eleven of the 35 participants performed with a negative energy balance of 2,000 Cal.d−1, and only five participants out of 35 participants performed at a less than 1,000 Cal.d−1 negative energy balance level. Conclusions Energy intake is not optimal as indicated by the negative energy balance and the low energy availability, which means that the participants to this selection course had to perform in suboptimal conditions.

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