scholarly journals CHARACTERIZATION OF A Microbacterium sp. STRAIN ISOLATED FROM SOILS CONTAMINATED WITH HYDROCARBONS IN THE BURGOS BASIN, MEXICO

2021 ◽  
Author(s):  
María Antonia Cruz-Hernández ◽  
Jessica Reyes-Peralta ◽  
Alberto Mendoza-Herrera ◽  
Gildardo Rivera ◽  
Virgilio Bocanegra-García
Keyword(s):  
Contaminated Soils ◽  
Rrna Gene ◽  
Soil Bioremediation ◽  
Cell Free Extract ◽  
Isolation And Identification ◽  
Surfactant Production ◽  
A Cell ◽  
The 16S Rrna Gene ◽  
Potential Use

The development of novel bioremediation strategies has focused on the isolation and identification of microorganisms that can thrive in polluted environments to evaluate their potential as biotechnological tools in bioremediation techniques. In this work, a bacterium isolated from hydrocarbon-contaminated soils from the Burgos basin was identified and its hydrocarbon degradation potential was evaluated. Identification based on sequencing the 16S rRNA gene identified one of the isolates (R3) as Microbacterium petrolearium. This strain was mainly antibiotic-sensitive with elevated carbohydrate assimilation differing from previously reported strains. Moderate surfactant production (I24 = 22.97 %) was observed, which was absent in a cell-free extract. M. petrolearium R3 showed increased growth that correlated with pollutant concentration. For light crude oil, at a higher contaminant percentage, the R3 strain showed increased growth; however, in the case of diesel, no growth was detected. The aforementioned data indicate that M. petrolearium strain R3, isolated from local sources, has potential use as a tool for hydrocarbon-contaminated soil bioremediation.

scholarly journals Isolation and Identification ofAcholeplasmasp. from the Mud Crab,Scylla serrata

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Ji-Gang Chen ◽  
Dan Lou ◽  
Ji-Fang Yang
Keyword(s):  
Cell Wall ◽  
Scylla Serrata ◽  
Rrna Gene ◽  
Mud Crab ◽  
Isolation And Identification ◽  
A Cell ◽  
First Time ◽  
The 16S Rrna Gene ◽  
Ferment Glucose ◽  
Mud Crabs

For the first time, a mollicute-like organism (MLO) was cultured from moribund mud crabs (Scylla serrata) during an outbreak of clearwater disease in Zhejiang Province, China. The MLO displayed a fried-egg colony morphology in culture, did not possess a cell wall, and was not retained by 0.45 μm and 0.2 μm filters. It was able to ferment glucose, sucrose, lactose, and maltose, but it did not utilize arginine and urea. The MLO grew in the absence of bovine serum and was not susceptible to digitonin. Sequence analysis of the 16S rRNA gene revealed that this MLO had 99% identity withAcholeplasma laidlawiiPG-8A, which indicates that the organism isolated from mud crabs is a member of the genusAcholeplasma.

scholarly journals Molecular Characterization of anEndozoicomonas-Like Organism Causing Infection in the King Scallop (Pecten maximusL.)

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Irene Cano ◽  
Ronny van Aerle ◽  
Stuart Ross ◽  
David W. Verner-Jeffreys ◽  
Richard K. Paley ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Mass Mortality ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
The 16S Rrna Gene

ABSTRACTOne of the fastest growing fisheries in the UK is the king scallop (Pecten maximusL.), also currently rated as the second most valuable fishery. Mass mortality events in scallops have been reported worldwide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were recorded in the Lyme Bay marine protected area (MPA) in Southwest England. Histopathological examination showed gill epithelial tissues infected with intracellular microcolonies (IMCs) of bacteria resemblingRickettsia-like organisms (RLOs), often with bacteria released in vascular spaces. Large colonies were associated with cellular and tissue disruption of the gills. Ultrastructural examination confirmed the intracellular location of these organisms in affected epithelial cells. The 16S rRNA gene sequences of the putative IMCs obtained from infected king scallop gill samples, collected from both mortality events, were identical and had a 99.4% identity to 16S rRNA gene sequences obtained from “CandidatusEndonucleobacter bathymodioli” and 95% withEndozoicomonasspecies.In situhybridization assays using 16S rRNA gene probes confirmed the presence of the sequenced IMC gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing, and bioinformatic analysis identified over 1,000 genes with high similarity to protein sequences fromEndozoicomonasspp. (ranging from 77 to 87% identity). Specific PCR assays were developed and applied to screen for the presence of IMC 16S rRNA gene sequences in king scallop gill tissues collected at the Lyme Bay MPA during 2015 and 2016. There was 100% prevalence of the IMCs in these gill tissues, and the 16S rRNA gene sequences identified were identical to the sequence found during the previous mortality event.IMPORTANCEMolluscan mass mortalities associated with IMCs have been reported worldwide for many years; however, apart from histological and ultrastructural characterization, characterization of the etiological agents is limited. In the present work, we provide detailed molecular characterization of anEndozoicomonas-like organism (ELO) associated with an important commercial scallop species.

Detection and Characterization of a Multi-drug and Multi-metal Resistant Enterobacterium Pantoea sp. from Tannery Wastewater after Secondary Treatment Process

2016 ◽  
Vol 2 (1 and 2) ◽  
pp. 37-42 ◽  
Author(s):  
Ashutosh Yadav ◽  
Abhay Raj ◽  
Ram Naresh Bharagava
Keyword(s):  
Toxic Metal ◽  
Tannery Wastewater ◽  
Contaminated Sites ◽  
Diffusion Method ◽  
Rrna Gene ◽  
Disk Diffusion Method ◽  
Gene Sequence Analysis ◽  
Nutrient Agar Medium ◽  
The 16S Rrna Gene

In this study, an enterobacterium was isolated from treated tannery wastewater and characterized as gram-negative, rod shaped, motile, and lactose fermenting bacterium. Further, based on the 16S rRNA gene sequence analysis, the bacterium was identified as Pantoea sp. with accession number The antibiotic and heavy KJ576899. metal resistant property of isolated bacterium was investigated by the disk diffusion method on Muller-Hinton and nutrient agar medium amended with the increasing concentrations of various toxic metal ions, respectively. Results showed that the isolated bacterium was sensitive for amikacin, ampicillin, cefepime, cetriaxone, chloramphenicol, levofloxacin, meropenem, nalidixic acid, piperacillin and tobramycin, resistant for aztreonam, carbenicillin, cefotaxime, ceftazidime, ciprofloxacin, cotrimoxazole, imepenam, moxifloxacin, streptomycin and tetracycline, but intermediate for amoxicillin and gentamicin. In addition, the bacterium also showed the Minimum Inhibitory Concentration (MIC) values of 250, 500, 160, 190, 600, 700, 500, 380, 600 and 350 μg ml-1 forCu, Cr, Cd, Co, Zn, Fe, Ni, Pb, Mo and As, respectively with a plasmid DNA of 33.5 kb. This multi-drug and multi-metal resistant bacterium can be used as a potential agent for the bioremediation of metal contaminated sites.

scholarly journals Phenotypic characterization of Sodalis praecaptivus sp. nov., a close non-insect-associated member of the Sodalis-allied lineage of insect endosymbionts

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1400-1405 ◽  
Author(s):  
Abhishek Chari ◽  
Kelly F. Oakeson ◽  
Shinichiro Enomoto ◽  
D. Grant Jackson ◽  
Mark A. Fisher ◽  
...  
Keyword(s):  
Novel Species ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Sodalis Glossinidius ◽  
Large Gene ◽  
Close Relationship ◽  
Metabolic Properties ◽  
Insect Symbionts ◽  
The 16S Rrna Gene

A Gram-stain-negative bacterium, isolated from a human wound was previously found to share an unprecedentedly close relationship with Sodalis glossinidius and other members of the Sodalis-allied clade of insect symbionts. This relationship was inferred from sequence analysis of the 16S rRNA gene and genomic comparisons and suggested the strain belonged to a novel species. Biochemical and genetic analyses supported this suggestion and demonstrated that the organism has a wide repertoire of metabolic properties, which is consistent with the presence of a relatively large gene inventory. Among members of the Sodalis-allied clade, this is the first representative that has sufficient metabolic capabilities to sustain growth in minimal media. On the basis of the results of this study, we propose that this organism be classified as a representative of a novel species, Sodalis praecaptivus sp. nov. (type strain HST = DSM 27494T = ATCC BAA-2554T).

Ruegeria pelagia sp. nov., isolated from the Sargasso Sea, Atlantic Ocean

2007 ◽  
Vol 57 (8) ◽  
pp. 1815-1818 ◽  
Author(s):  
Kiyoung Lee ◽  
Yoe-Jin Choo ◽  
Stephen J. Giovannoni ◽  
Jang-Cheon Cho
Keyword(s):  
Phylogenetic Analyses ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Sargasso Sea ◽  
Bacterial Strains ◽  
Ruegeria Pomeroyi ◽  
Distinct Line ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

Gram-negative, facultatively aerobic, chemoheterotrophic, short rod-shaped marine bacterial strains HTCC2662T and HTCC2663, isolated from the Sargasso Sea by using a dilution-to-extinction culturing method, were investigated to determine their taxonomic position. Characterization of the two strains by phenotypic and phylogenetic analyses revealed that they belonged to the same species. The DNA G+C content of strain HTCC2662T was 58.4 mol% and the predominant cellular fatty acids were C18 : 1 ω7c (52.5 %), C16 : 0 2-OH (13.5 %) and C18 : 1 11-methyl ω7c (12.2 %). Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains represented a distinct line of descent within the genus Ruegeria, with highest sequence similarities to Ruegeria atlantica DSM 5823T (97.2 %), Ruegeria lacuscaerulensis DSM 11314T (96.5 %) and Ruegeria pomeroyi DSM 15171T (95.6 %). Several phenotypic characteristics, including facultatively requiring NaCl and oxygen for growth, together with the cellular fatty acid composition, differentiated strain HTCC2662T from other members of the genus Ruegeria. Based on phenotypic, chemotaxonomic and phylogenetic traits, it is suggested that strains HTCC2662T and HTCC2663 represent a novel species of the genus Ruegeria, for which the name Ruegeria pelagia sp. nov. is proposed. The type strain is HTCC2662T (=KCCM 42378T=NBRC 102038T).

Isolation and Identification of a New Pathogen Causing Mulberry Bacterial Wilt Disease

2011 ◽  
Vol 282-283 ◽  
pp. 526-530
Author(s):  
Xing Jiang ◽  
Fu An Wu ◽  
Shui Qin Fang ◽  
Yan Dong Zhang ◽  
Jun Wang ◽  
...  
Keyword(s):  
Plant Disease ◽  
Gene Sequence ◽  
Hypersensitive Reaction ◽  
Wilt Disease ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
Physiological Characterization ◽  
Bacterial Wilt Disease ◽  
Gene Sequence Analysis ◽  
The 16S Rrna Gene

To identify the pathogen of Mulberry wilt disease, a tested strain WJ-1 was isolated from a naturally infected mulberry in Guangzhou, Guangdong province, P. R. China. Hypersensitive reaction, along with Koch’s postulates, morphological, biochemical and physiological characterization, light microscope observation and molecular identification were performed on WJ-1. The results indicated that WJ-1 was a Gram-negative, short-rod shaped bacterium. Based on morphology and the 16S rRNA gene sequence analysis, WJ-1 strain was identified as members of the genusEnterobacter. The phylogenetic tree revealed that WJ-1 shared the highest homology withEnterobacter cloacaestrain M5. It was another evidence on plant disease caused byEnterobacterspecies. We found thatEnterobacterspecies could cause mulberry wilt disease in Guangzhou.

scholarly journals MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS IN SPONGE AT MELAWAI BEACH, EAST KALIMANTAN

2018 ◽  
Author(s):  
Ismail Marzuki ◽  
Alfian Noor ◽  
Nursiah La Nafie
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Morphological Identification ◽  
Rrna Gene ◽  
Isolation And Purification ◽  
East Kalimantan ◽  
Degree Of Similarity ◽  
The 16S Rrna Gene

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histo- morphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp. Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous

scholarly journals Isolation of a Leuconostoc mesenteroides ssp. jonggajibkimchii strain from Parona leatherjacket (Parona signata): behavior in vegetal matrices fermentation

2022 ◽  
Vol 75 (1) ◽  
Author(s):  
Romina Belén Parada ◽  
Franco M. Sosa ◽  
Emilio R. Marguet ◽  
Marisol Marguet
Keyword(s):  
Chinese Cabbage ◽  
Sequence Similarity ◽  
Skim Milk ◽  
Rrna Gene ◽  
Spontaneous Fermentation ◽  
White Cabbage ◽  
Vancomycin Resistant ◽  
The 16S Rrna Gene ◽  
Potential Use ◽  
Selection Of

A Gram-positive, facultatively anaerobic, gas-forming, catalase-negative, nonmotile, non-sporeforming, vancomycin-resistant, and ovoid-shaped bacterium, designated strain Tw234, was isolated from the intestinal tract of Parona leatherjacket (Parona signata). The strain grew in the presence of 0–6% (w/v) NaCl, at pH 3.5–8.5 and 8–40 °C; optimum growth was achieved at 1% (w/v) NaCl, at pH 6.0 and 30–32 °C. Exopolysacharides production was detected by the solidification test of skim milk supplemented with sucrose in the temperature range of 8 to 30 °C. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indicated that strain Tw234 was closed related to the genus Leuconostoc and 100% homology with the type strain Ln. mesenteroides ssp. jonggajibkimchii DRC1506 (KCCM 43249, JCM 31787). The evaluation of growth and acidification rates were carried out in white cabbage and Chinese cabbage and compared with the strain Ln. mesenteroides ssp. jonggajibkimchii RCTw1.1, isolated from the spontaneous fermentation of red cabbage. No significant differences were observed between the behaviors of the two strains. The strain Tw234 displayed higher growth and acidification rates in controlled fermentation of white cabbage compared with those obtained in Chinese cabbage. New trends are targeted on the isolation and selection of strains to achieve controlled fermentation of vegetables that may ensure uniform quality. The results obtained in this work suggest that strain Tw234 harbored technological useful properties for its potential use as a starter in controlled vegetable fermentations.

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