Urinary Equol is Associated with Bioavailable Testosterone but not Total Testosterone in Women

2019 ◽  
Author(s):  
Hong-li Dong ◽  
Feng Xiong ◽  
Qing-wei Zhong ◽  
Yi-hong Li ◽  
Meng Liu ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
Menopausal Status ◽  
Premenopausal Women ◽  
Positive Association ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Total Testosterone ◽  
Significant Positive Association ◽  
Cross Sectional ◽  
Bioavailable Testosterone

Little is known about the association between equol and bioavailable testosterone (BT) in adults. We examined the associations of urinary equol concentrations with serum total, bioavailable and free testosterone (FT), dehydroepiandrosterone sulfide (DHEAS), free androgen index (FAI) and sex hormone-binding globulin (SHBG) concentrations. This cross-sectional study included 1904 women aged 59.7 years. Urinary equol and serum sex hormone concentrations were measured. Overall, urinary equol tended to be inversely associated with bioactive forms of androgenic indices (BT, FT or FAI) but not with total testosterone (TT) or DHEAS. Urinary equol was also positively associated with SHBG. In multi-covariate-adjusted analyses stratified by menopausal status, graded and inverse associations between urinary equol and bioactive forms of androgenic indices (BT, FT and FAI) were observed in postmenopausal women (all p-trends <0.05), but not in premenopausal women. A significant positive association between urinary equol and SHBG was observed only in postmenopausal women. No significant associations were observed between urinary equol and TT or DHEAS in either group. A path analysis indicated that these associations of equol with androgens in postmenopausal women might be mediated by SHBG. Our findings indicated urinary equol exhibited graded and inverse associations with BT or FT but not TT in women.

scholarly journals The Association Between Clinical and Biochemical Hyperandrogenism in Women With Female Pattern Hair Loss

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A745-A746
Author(s):  
Samih A Odhaib ◽  
Abbas A Mansour ◽  
Khalil I Al Hamdi
Keyword(s):  
Hair Loss ◽  
Significant Relationship ◽  
Premenopausal Women ◽  
Sex Hormone Binding Globulin ◽  
Diagnostic Process ◽  
Total Testosterone ◽  
Reference Ranges ◽  
Cross Sectional ◽  
Bioavailable Testosterone ◽  
Female Pattern Hair Loss

Abstract Background: The exact association between clinical and biochemical hyperandrogenism (HA) is heterogeneous and cannot be ascertained, especially in normoandrogenic women. Aim: Evaluate any association between clinical HA phenotypes and biochemical parameters in premenopausal women with female pattern hair loss (FPHL). Methods: A cross-sectional observational study on 362 women with different degrees of FPHL, who were assessed for general characteristics, the degree of FPHL by Sinclair’s score, hirsutism by modified Ferriman-Gallwey (mFG) score. Evaluation for biochemical HA included total testosterone (TT), sex-hormone-binding globulin (SHBG), calculated free testosterone (FT), calculated bioavailable testosterone (BT), and dehydroepiandrosterone sulfate (DHEA-S). The variables of clinical HA which were used in this study are FPHL, hirsutism, and acne. We used the Free and Bioavailable Testosterone Calculator to calculate the FT and BT. Results: The enrolled young premenopausal women’s age range was (14-47 years). Around 78% of them were overweight or obese. Eighty-percent of women had a mild FPHL, with a median duration of three years where 2/3 of women had a duration < 3 years, and had no significant relationship to FPHL degree. About 73% of women had either a mild to moderate hirsutism, and around 16% had acne. The biochemical HA was confirmed in around 52% of women (n=188), who show high levels of calculated FT. The calculated BT is high in 78.5% of the enrolled women (n=284). The means of biochemical indicators for HA were in their reference ranges or slightly above, with no specific change pattern with the corresponding FPHL severity. None of these parameters had a significant relationship to the severity of FPHL. The duration of FPHL was not affected by any presumed variable of clinical or biochemical HA. Conclusions: FPHL severity is associated with other clinical HA signs like hirsutism and acne, but not to HA’s biochemical parameter. Other parameters, like SHBG, HOMA-IR, and BMI, had no significant relation to the severity of FPHL. Clinical implications: FPHL severity does not correlate with the magnitude of hyperandrogenism. The assessment of women with FPHL is primarily clinical. The biochemical picture assists the diagnostic process.

scholarly journals Association among age, gender, menopausal status and small dense low-density lipoprotein cholesterol: a cross-sectional study

BMJ Open ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. e041613
Author(s):  
Toshihide Izumida ◽  
Yosikazu Nakamura ◽  
Yukihiro Sato ◽  
Shizukiyo Ishikawa
Keyword(s):  
Postmenopausal Women ◽  
Low Density Lipoprotein ◽  
Menopausal Status ◽  
Density Lipoprotein ◽  
Premenopausal Women ◽  
Cross Sectional Study ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Cross Sectional

ObjectivesSmall dense low-density lipoprotein cholesterol (sdLDL-C) might be a better cardiovascular disease (CVD) indicator than low-density lipoprotein cholesterol (LDL-C); however, details regarding its epidemiology remain elusive. The present study aimed at evaluating the association between the demographic factors, such as age, gender and menopausal status, and sdLDL-C levels and sdLDL-C/LDL-C ratio in the Japanese population.DesignThis was a cross-sectional study.Setting13 rural districts in Japan, 2010–2017.ParticipantsThis study included 5208 participants (2397 men and 2811 women), who underwent the health mass screening that was conducted in accordance with the medical care system for the elderly and obtained informed consent for this study.ResultsIn total, 517 premenopausal women (mean age ±SD, 45.1±4.2 years), 2294 postmenopausal women (66.5±8.8 years) and 2397 men (64.1±11.2 years) were analysed. In men, the sdLDL-C levels and sdLDL-C/LDL-C ratio increased during younger adulthood, peaked (36.4 mg/dL, 0.35) at 50–54 years, and then decreased. In women, relatively regular increasing trends of sdLDL-C level and sdLDL-C/LDL-C ratio until approximately 65 years (32.7 mg/dL, 0.28), followed by a downward or pleated trend. Given the beta value of age, body mass index, fasting glucose and smoking and drinking status by multiple linear regression analysis, standardised sdLDL-C levels and sdLDL-C/LDL-C ratio in 50-year-old men, premenopausal women and postmenopausal women were 26.6, 22.7 and 27.4 mg/dL and 0.24, 0.15 and 0.23, respectively. The differences between premenopausal and postmenopausal women were significant (p<0.001).ConclusionsSdLDL-C and sdLDL-C/LDL-C ratios showed different distributions by age, gender and menopausal status. A subgroup-specific approach would be necessary to implement sdLDL-C for CVD prevention strategies, fully considering age-related trends, gender differences and menopausal status.

Association of Daytime Napping and Diagnosed Diabetes in Middle-Aged Premenopausal, Middle-Aged Postmenopausal, and Older Postmenopausal Chinese Women

2019 ◽  
Vol 33 (8) ◽  
pp. 1107-1114 ◽  
Author(s):  
Shu Fang ◽  
Junmin Zhou
Keyword(s):  
Postmenopausal Women ◽  
Chinese Women ◽  
Statistical Significance ◽  
Menopausal Status ◽  
Premenopausal Women ◽  
Total Sample ◽  
Middle Aged ◽  
Cross Sectional ◽  
Diagnosed Diabetes ◽  
Stratified Analysis

Purpose: To examine associations of daytime napping and diagnosed diabetes in middle-aged premenopausal, middle-aged postmenopausal, and older postmenopausal Chinese women. Design: Quantitative, cross-sectional. Setting: 2015 cross-sectional data from China Health and Retirement Longitudinal Study. Participants were recruited from 150 counties/districts and 450 villages/resident committees. Participants: Six thousand nine hundred and forty women aged 45 years and older (mean age = 61 years) stratified by age and menopausal status. Measures: The outcome was self-reported diagnosed diabetes. The exposure was self-reported daytime napping (0, >0-≤60, or >60 min/d). Participants were stratified to middle-aged premenopausal, middle-aged postmenopausal, and older postmenopausal women according to their age (≤60 or >60 years) and menopausal status. Analysis: One-way analysis of variance and χ2 tests were conducted to explore differences on characteristics of middle-aged premenopausal, middle-aged postmenopausal, and older postmenopausal women. Multiple logistic regressions were used to estimate adjusted odds ratios (ORs) for diagnosed diabetes according to daytime napping in the total sample, middle-aged premenopausal, middle-aged postmenopausal, and older postmenopausal Chinese women. Results: Participants’ mean self-reported daytime napping duration was 34 minutes. Women who napped more than 60 minutes were more likely to report diagnosed diabetes (OR = 1.39, 95% confidence interval (CI), 1.09-1.76) comparing to those who did not nap, after adjusting for potential confounders. No statistical significance of interaction term between daytime napping and age/menopausal status was detected ( P = .602 and P = .558) among total women. The stratified analysis revealed the significant association among middle-aged postmenopausal women napping more than 60 minutes (OR = 1.81, 95% CI, 1.18-2.77). The association, however, was found to be insignificant in middle-aged premenopausal women and older postmenopausal women. Conclusions: Long daytime nap (>60 min/d) was associated with diagnosed diabetes in middle-aged postmenopausal women in China.

scholarly journals Adherence to the low carbohydrate diet and the risk of breast Cancer in Iran

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Bahareh Sasanfar ◽  
Fatemeh Toorang ◽  
Ahmad Esmaillzadeh ◽  
Kazem Zendehdel
Keyword(s):  
Breast Cancer ◽  
Postmenopausal Women ◽  
Premenopausal Women ◽  
Positive Association ◽  
Carbohydrate Diet ◽  
Significant Positive Association ◽  
Low Carbohydrate Diet ◽  
Low Carbohydrate ◽  
Study Population ◽  
Study Participants

Abstract Background Previous studies on the link between macronutrients and breast cancer have mostly focused on individual macronutrients rather than their combination. This study investigates the association between adherence to a low carbohydrate diet and odds of breast cancer among women. Methods This hospital-based case-control study was carried out on 412 women with pathologically confirmed breast cancer within the past year and 456 apparently healthy controls that were matched in terms of age and residential place. Dietary data was collected using a 168-item validated FFQ. Participants were classified in terms of quintiles of percentages of energy intake from carbohydrates, proteins, and fats. Then, individuals in the highest quintile of fat and protein intake were given a score of 5 and those in the lowest quintile of these macronutrients were given a score of 1. Participants in the other quintiles of these macronutrients were given the corresponding score. In terms of carbohydrate intake, those in the highest quintile received a score of 1 and those in the lowest quintile received 5. The scores were then summed up to calculate the total low carbohydrate diet (LCD) score, which varied from 3 to 15. A higher score meant greater adherence to a low carbohydrate diet. Results The mean age of study participants was 45.2 y and mean BMI was 28.4 kg/m2. Mean LCD score of participants was 8.9 ± 2.5 (8.9 ± 2.6 in cases and 9.0 ± 2.5 in controls). Although no significant association was observed between adherence to the LCD score and odds of breast cancer in the study population, a trend toward significant positive association was seen between consumption of LCD and odds of breast cancer in postmenopausal women; after controlling for several potential confounders, individuals in the third quartile of LCD score were 1.94 times more likely to have breast cancer than those in the lowest quartile (95% CI: 1.00, 3.76). This association strengthened after controlling for dietary variables (2.50; 1.18–5.32). Even after further adjustment for BMI, this association remained significant (2.64, 1.23–5.67). No significant relationship was observed in premenopausal women, either before or after controlling for confounders. Conclusion Adherence to LCD may be associated with increased odds of breast cancer in postmenopausal women. Prospective cohort studies are needed to confirm these findings.

scholarly journals Measures of bioavailable serum testosterone and estradiol and their relationships with muscle mass, muscle strength and bone mineral density in postmenopausal women: a cross-sectional study

2009 ◽  
Vol 160 (4) ◽  
pp. 681-687 ◽  
Author(s):  
Tineke A C M van Geel ◽  
Piet P Geusens ◽  
Bjorn Winkens ◽  
Jean-Pierre J E Sels ◽  
Geert-Jan Dinant
Keyword(s):  
Bone Mineral Density ◽  
Muscle Strength ◽  
Postmenopausal Women ◽  
Bone Mineral ◽  
Handgrip Strength ◽  
Sex Hormone Binding Globulin ◽  
Total Testosterone ◽  
Mineral Density ◽  
Bioavailable Testosterone ◽  
Age Related

ObjectiveThe physiologic role of circulating endogenous testosterone and estrogen concentrations in relation to lean body mass (LBM) and muscle strength is not as well documented in postmenopausal women as in elderly men.DesignThree hundred and twenty-nine healthy postmenopausal women were randomly selected from a general practice population-based sample aged between 55 and 85 years.MethodsTotal testosterone and estrogen (TT and TE) and sex hormone-binding globulin (SHBG) were determined and estimates of bioavailable testosterone (free androgen index (TT/SHBG, FAI), calculated free testosterone (cFT), and estrogen (TE/SHBG, ESR) were calculated. Examinations included bone mineral density (BMD) of the spine and femoral neck (FN), LBM, maximum quadriceps extension strength (MES) and maximum handgrip strength (MGS), timed up-and-go test (TUGT), osteocalcin (OC), and urinary deoxy-pyridinoline/creatinine (DPyr). Correlations were assessed using Pearson's correlation coefficient (r).ResultsWith advancing age, LBM, MES, MGS, BMD, and ESR significantly declined (ranger: −0.356 to −0.141) and TUGT, and DPyr significantly increased (ranger: 0.135 to 0.282 (P<0.05)). After age-adjustment, LBM, MES, and BMD in spine and FN were significantly related to bioavailable testosterone (ranger: 0.146 to 0.193, for cFT, and 0.157 to 0.224, for FAI) and to ESR (ranger: 0.162 to 0.273). OC and DPyr were significantly inversely related to ESR (r: −0.154 and −0.144 respectively).ConclusionsAge-related loss of LBM, MES and BMD in postmenopausal women is partly dependent on the presence of endogenous bioavailable testosterone and estrogen.

scholarly journals Sex Hormone–Binding Globulin and Risk of Clinical Diabetes in American Black, Hispanic, and Asian/Pacific Islander Postmenopausal Women

2012 ◽  
Vol 58 (10) ◽  
pp. 1457-1466 ◽  
Author(s):  
Brian H Chen ◽  
Kathleen Brennan ◽  
Atsushi Goto ◽  
Yiqing Song ◽  
Najib Aziz ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
Diabetes Risk ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Clinical Diabetes ◽  
Total Testosterone ◽  
Inverse Association ◽  
Hormone Binding ◽  
Strong Inverse Association ◽  
Asian Pacific

Abstract BACKGROUND Recent prospective studies have shown a strong inverse association between sex hormone–binding globulin (SHBG) concentrations and risk of clinical diabetes in white individuals. However, it remains unclear whether this relationship extends to other racial/ethnic populations. METHODS We evaluated the association between baseline concentrations of SHBG and clinical diabetes risk in the Women's Health Initiative Observational Study. Over a median follow-up of 5.9 years, we identified 642 postmenopausal women who developed clinical diabetes (380 blacks, 157 Hispanics, 105 Asians) and 1286 matched controls (777 blacks, 307 Hispanics, 202 Asians). RESULTS Higher concentrations of SHBG at baseline were associated with a significantly lower risk of clinical diabetes [relative risk (RR), 0.15; 95% CI, 0.09–0.26 for highest vs lowest quartile of SHBG, adjusted for BMI and known diabetes risk factors]. The associations remained consistent within ethnic groups [RR, 0.19 (95% CI, 0.10–0.38) for blacks; RR, 0.17 (95% CI, 0.05–0.57) for Hispanics; and 0.13 (95% CI, 0.03–0.48) for Asians]. Adjustment for potential confounders, such as total testosterone (RR, 0.11; 95% CI, 0.07–0.19) or HOMA-IR (RR, 0.26; 95% CI, 0.14–0.48) did not alter the RR substantially. In addition, SHBG concentrations were significantly associated with risk of clinical diabetes across categories of hormone therapy use (never users: RRper SD = 0.42, 95% CI, 0.34–0.51; past users: RRper SD = 0.53;, 95% CI, 0.37–0.77; current users: RRper SD = 0.57; 95% CI, 0.46–0.69; P-interaction = 0.10). CONCLUSIONS In this prospective study of postmenopausal women, we observed a robust, inverse relationship between serum concentrations of SHBG and risk of clinical diabetes in American blacks, Hispanics, and Asians/Pacific Islanders. These associations appeared to be independent of sex hormone concentrations, adiposity, or insulin resistance.

scholarly journals Determination of Bioavailable Testosterone [Non–Sex Hormone–Binding Globulin (SHBG)-Bound Testosterone] in a Population of Healthy French Men: Influence of Androstenediol on Testosterone Binding to SHBG

2007 ◽  
Vol 53 (12) ◽  
pp. 2160-2168 ◽  
Author(s):  
Frank Giton ◽  
Saïk Urien ◽  
Catherine Born ◽  
Jean Tichet ◽  
Jérôme Guéchot ◽  
...  
Keyword(s):  
Binding Constants ◽  
Normal Serum ◽  
Association Constants ◽  
Sex Hormone ◽  
Mass Action ◽  
Sex Hormone Binding Globulin ◽  
Total Testosterone ◽  
Hormone Binding ◽  
Bioavailable Testosterone ◽  
Age Related

Abstract Background: Bioavailable testosterone (BT) is measured [assayed BT (aBT)] or calculated (cBT) in the diagnosis of hypogonadism in men. The cBT depends, however, on the values of the association constants of total testosterone (TT) for sex hormone–binding globulin (SHBG; Ks) and albumin (Ka), and its use therefore remains controversial. Methods: In 503 selected, untreated healthy men, 20–74 years old, we measured TT, dihydrotestosterone (DHT), and androstenediol (5-diol) by GC-MS, SHBG by RIA, and BT after ammonium sulfate precipitation or by calculation according to the law of mass action. Results: A slight decrease in TT, significant decreases in BT and 5-diol, no variation in DHT, and an increase in SHBG were observed with age. In young males (≤39 years), the lower normal limits were between 2.30 and 2.72 nmol/L for aBT and 8.50 nmol/L for TT. For Ks = 1 × 109 L/mol and Ka = 3.6 × 104 L/mol, the lower cBT limit was found to be 2-fold higher than for aBT. With optimized Ks = 1.9 × 109 L/mol and Ka = 2.45 × 104 L/mol, cBT values close to aBT were obtained. When 5-diol was included in the model as a competitive SHBG inhibitor, the correlation between cBT and aBT was better and the cBT:aBT ratios vs 5-diol were less biased. Conclusion: Lower normal serum aBT concentration in normal men appears to be between 2.30 and 2.72 nmol/L. Much higher serum cBT concentrations are associated with use of different association constants that may be inappropriate. When using the optimized binding constants, taking age-related 5-diol values into consideration slightly improves prediction of cBT.

scholarly journals Assessment of FMO3 SNPs in Relation to TMAO in Generally Healthy United States Adults

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 940-940
Author(s):  
Kristen James ◽  
Erik Gertz ◽  
Catherine Kirschke ◽  
Liping Huang ◽  
Charles Stephensen ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
Cystatin C ◽  
A Priori ◽  
Menopausal Status ◽  
Premenopausal Women ◽  
Cross Sectional Study ◽  
Full Cohort ◽  
Cross Sectional ◽  
Relationship Of ◽  
The Relationship

Abstract Objectives The hepatic enzyme flavin monooxygenase 3 (FMO3) oxidizes many metabolites including trimethylamine to the atherogenic molecule trimethylamine n-oxide (TMAO). Variants in the open reading frame of the FMO3 gene alter the enzyme's activity; therefore, we genotyped two a priori missense FMO3 SNPs in a cohort of unmedicated healthy adults. We hypothesized that the SNPs might affect the activity of the encoded enzyme leading to reductions in circulating TMAO. FMO3 expression is upregulated by estrogen, thus we also assessed the relationship of the SNPs and TMAO in pre- and postmenopausal women. Methods DNA was extracted from whole blood from 349 subjects (182 women) who were enrolled in a cross-sectional study at the USDA/ARS WHNRC. SNPs rs2266782 (G &gt; A, p.Glu158Lys) and rs2266780 (A &gt; G, p.Glu308Gly) were genotyped using TaqMan SNP genotyping kits and PCR. TMAO was purified from fasted plasma and quantified using high resolution LC-MS. Regression models were built to assess the relationship of the SNPs to TMAO in the full cohort and by self-reported menopausal status in women. Models assessing the full cohort were adjusted for plasma cystatin C and a sex*age interaction, whereas the menopausal analysis was adjusted for cystatin C. Results The cohort's minor allele frequencies were 36.5% and 17.5% for SNPs rs2266782 and rs2266780, respectively, which were consistent with the genome aggregation exome reports. For both SNPs, median TMAO concentrations increased in individuals carrying the risk alleles, however the differences by genotypes were not significant. In women, the AA genotype at rs2266780 was associated with reduced TMAO levels in pre-, but not postmenopausal women (P = 0.01). This effect was not identified in females with AG or GG genotypes, regardless of their menopausal state. Conclusions Effects of the evaluated FMO3 SNPs on TMAO levels were not identified in the full cohort. However, the SNP rs2266780 was associated with reduced TMAO in premenopausal women with the AA genotype but not women with the AG or GG genotypes, nor those who were postmenopausal. This finding reinforces previous observations that risks for cardiovascular diseases increase after menopause in women. Funding Sources The Beef Checkoff, R01HL128572; USDA-ARS 2032–53,000–001–00-D, 2032–51,530–022–00-D, and 2032–51,000-004–00D; NCATS NIH UL1 TR001860.

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