scholarly journals Influence of Ascorbic Acid against Salt stress on the Germination, Biochemicals and Micronutrients Concentrations of Solanum melongena (L.)

Author(s):  
Muhammad Irfan ◽  
Gul Jan ◽  
Farzana Gul Jan ◽  
Waheed Murad

The experiments were designed to know the function of ascorbic acid against salinity on germination, different biochemical and micronutrients concentrations of Solanum melongena. Ascorbic acid was applied as foliar spray on leaves and given in roots through irrigation at 100 and 200 ppm to the plants growed in non saline, 60mM and 100mM salt stress conditions. Different biochemical parameters i.e. chlorophyll, carotenoids, carbohydratrates, protein and micro nutrients concentrations of Solanum melongena were observed under different salt stress concentrations from control (non-saline), 60mM NaCl and 100mM NaCl solutions. The rates of different biochemical parameters and micro nutrients concentrations exhibited decrease in saline media in comparison with their respective control while ascorbic acid used as a foliar spray and through irrigation in roots at the concentration of 100 ppm and 200 ppm resulted promotion in non saline control as compared with salt concentrations media. It was determined that chlorophyll concentration was decreased considerably under salt stress. Amongst micro nutrients ionic composition of Na, K and Na/K ratio resulted that plants treated with different NaCl concentrations at 60mM NaCl and 100mM salt concentrations showed increase in Na and K ions and ascorbic acid were used as a foliar spray and in roots showed decrease in Na and K ions. Overall from the outcomes of the study it was observed that salinity stress significantly decrease vegetative and reproductive parameters that could be improved with the using of ascorbic acid. Best concentration found were 200 ppm ascorbic acid contributed towards an increase in growth irrespective of non-saline and saline conditions. Based on these findings, ascorbic acid treatment helps in alleviating the negative effects of salinity.

2021 ◽  
Vol 9 (6) ◽  
pp. 1209
Author(s):  
Nuria Montes-Osuna ◽  
Carmen Gómez-Lama Cabanás ◽  
Antonio Valverde-Corredor ◽  
Garikoitz Legarda ◽  
Pilar Prieto ◽  
...  

Stress caused by drought and salinity may compromise growth and productivity of olive (Olea europaea L.) tree crops. Several studies have reported the use of beneficial rhizobacteria to alleviate symptoms produced by these stresses, which is attributed in some cases to the activity of 1-aminocyclopropane-1-carboxylic acid deaminase (ACD). A collection of beneficial olive rhizobacteria was in vitro screened for ACD activity. Pseudomonas sp. PICF6 displayed this phenotype and sequencing of its genome confirmed the presence of an acdS gene. In contrast, the well-known root endophyte and biocontrol agent Pseudomonas simiae PICF7 was defective in ACD activity, even though the presence of an ACD-coding gene was earlier predicted in its genome. In this study, an unidentified deaminase was confirmed instead. Greenhouse experiments with olive ‘Picual’ plants inoculated either with PICF6 or PICF7, or co-inoculated with both strains, and subjected to drought or salt stress were carried out. Several physiological and biochemical parameters increased in stressed plants (i.e., stomatal conductance and flavonoids content), regardless of whether or not they were previously bacterized. Results showed that neither PICF6 (ACD positive) nor PICF7 (ACD negative) lessened the negative effects caused by the abiotic stresses tested, at least under our experimental conditions.


2015 ◽  
Vol 26 (2) ◽  
pp. 21-25 ◽  
Author(s):  
Marcelina Krupa-Małkiewicz ◽  
Beata Smolik ◽  
Dominik Ostojski ◽  
Maja Sędzik ◽  
Justyna Pelc

AbstractThe aim of this study is to determine the effect of both NaCl and KCl alone and in comparison to AsA on the morphological and some biochemical parameters of Oxheart and Vilma cultivars of tomato under laboratory and field conditions. A combination of salt applied in the laboratory experiment caused a significant effect on seed germination and root and shoot length and a significant reduction of Chl a, Chl b and Car contents in 14-day-old tomato seedlings. However, seedlings of cultivar Vilma were characterised by higher tolerance to applied salt stress.NaCl caused a significant decrease in Chl a, Chl b and Car, and an increase in Pro and MDA content in the leaves of Vilma cultivar under field conditions. Besides, tomato plants cv. Vilma treated with NaCl alone or NaCl with ascorbic acid developed longer roots, from 48 to 73%, compared to the control.


Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 684
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Mahdi Behnamian ◽  
Gholamreza Gohari ◽  
Mousa Torabi Giglou ◽  
...  

Salt stress severely reduces growth and yield of plants. Considering the positive effects of selenium (Se) and chitosan (Cs) separately against abiotic stress, in these experiments, we synthesized chitosan–selenium nanoparticles (Cs–Se NPs) and investigated their ability to reduce the negative effects of salt stress on growth and some biochemical parameters of bitter melon (Momordica charantia). Bitter melon plants were grown at three NaCl salinity levels (0, 50, and 100 mM) and a foliar spray of Cs–Se NPs (0, 10, and 20 mg L−1) was applied. Some key morphological, biochemical, and physiological parameters in leaf samples and essential oil from fruit were measured at harvest. Salinity decreased growth and yield while foliar application of Cs–Se NPs increased these critical parameters. Furthermore, Cs–Se NPs enhanced bitter melon tolerance to salinity by increasing antioxidant enzyme activity, proline concentration, relative water content, and K+, and decreasing MDA and H2O2 oxidants and Na aggregation in plant tissues. Yield was also improved, as the highest amount of essential oils was produced by plants treated with Cs–Se NPs. Generally, the greatest improvement in measured parameters under saline conditions was obtained by treating plants with 20 mg L−1 Cs–Se NPs, which significantly increased salinity tolerance in bitter melon plants.


Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1316
Author(s):  
Abida Parveen ◽  
Muhammad Arslan Ashraf ◽  
Iqbal Hussain ◽  
Shagufta Perveen ◽  
Rizwan Rasheed ◽  
...  

The present work reports the assessment of the effectiveness of a foliar-spray of salicylic acid (SA) on growth attributes, biochemical characteristics, antioxidant activities and osmolytes accumulation in wheat grown under control (100% field capacity) and water stressed (60% field capacity) conditions. The total available water (TAW), calculated for a rooting depth of 1.65 m was 8.45 inches and readily available water (RAW), considering a depletion factor of 0.55, was 4.65 inches. The water contents corresponding to 100 and 60% field capacity were 5.70 and 1.66 inches, respectively. For this purpose, seeds of two wheat cultivars (Fsd-2008 and S-24) were grown in pots subjected to water stress. Water stress at 60% field capacity markedly reduced the growth attributes, photosynthetic pigments, total soluble proteins (TSP) and total phenolic contents (TPC) compared with control. However, cv. Fsd-2008 was recorded as strongly drought-tolerant and performed better compared to cv. S-24, which was moderately drought tolerant. However, water stress enhanced the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and membrane electrolyte leakage (EL) and modulated the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as accumulation of ascorbic acid (AsA), proline (Pro) and glycine betaine (GB) contents. Foliar-spray with salicylic acid (SA; 0, 3 mM and 6 mM) effectively mitigated the adverse effects of water stress on both cultivars. SA application at 6 mM enhanced the shoot and root length, as well as their fresh and dry weights, and improved photosynthetic pigments. SA foliage application further enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and nonenzymatic antioxidants such as ascorbic acid and phenolics contents. However, foliar-spray of SA reduced MDA, H2O2 and membrane permeability in both cultivars under stress conditions. The results of the present study suggest that foliar-spray of salicylic acid was effective in increasing the tolerance of wheat plants under drought stress in terms of growth attributes, antioxidant defense mechanisms, accumulation of osmolytes, and by reducing membrane lipid peroxidation.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 835
Author(s):  
Jose Alvarez ◽  
Elvira Martinez ◽  
Belén Diezma

Hyperspectral imaging is an appropriate method to thoroughly investigate the microscopic structure of internally heterogeneous agro-food products. By using hyperspectral technology, identifying stress symptoms associated with salinity, before a human observer, is possible, and has obvious benefits. The objective of this paper was to prove the suitability of this technique for the analysis of Triticale seeds subjected to both magneto-priming and drought and salt stress conditions, in terms of image differences obtained among treatments. It is known that, on the one hand, drought and salt stress treatments have negative effects on seeds of almost all species, and on the other hand, magneto-priming enhances seed germination parameters. Thus, this study aimed to relate hyperspectral imaging values—neither positive nor negative in themselves—to the effects mentioned above. Two main conclusions were reached: Firstly, the hyperspectral application is a feasible method for exploring the Triticale structure and for making distinctions under different drought and salt stress treatments, in line with the data variability obtained. Secondly, the lower spectral reflectance in some treatments—in the 400–1000 nm segment—is the result of a great number of chemical compounds in the seed that could be related to magneto-priming.


Author(s):  
Marija Bursać-Mitrović ◽  
Dragan R. Milovanović ◽  
Radoslav Mitić ◽  
Danijela Jovanović ◽  
Miroslav Sovrlić ◽  
...  

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