scholarly journals Effect of ascorbic acid on morphological and biochemical parameters in tomato seedling exposure to salt stress

2015 ◽  
Vol 26 (2) ◽  
pp. 21-25 ◽  
Author(s):  
Marcelina Krupa-Małkiewicz ◽  
Beata Smolik ◽  
Dominik Ostojski ◽  
Maja Sędzik ◽  
Justyna Pelc
Keyword(s):  
Ascorbic Acid ◽  
Salt Stress ◽  
Biochemical Parameters ◽  
Field Conditions ◽  
Tomato Seedling ◽  
Tomato Plants ◽  
Chl A ◽  
Tomato Seedlings ◽  
Root And Shoot Length ◽  
Mda Content

AbstractThe aim of this study is to determine the effect of both NaCl and KCl alone and in comparison to AsA on the morphological and some biochemical parameters of Oxheart and Vilma cultivars of tomato under laboratory and field conditions. A combination of salt applied in the laboratory experiment caused a significant effect on seed germination and root and shoot length and a significant reduction of Chl a, Chl b and Car contents in 14-day-old tomato seedlings. However, seedlings of cultivar Vilma were characterised by higher tolerance to applied salt stress.NaCl caused a significant decrease in Chl a, Chl b and Car, and an increase in Pro and MDA content in the leaves of Vilma cultivar under field conditions. Besides, tomato plants cv. Vilma treated with NaCl alone or NaCl with ascorbic acid developed longer roots, from 48 to 73%, compared to the control.

scholarly journals Silencing of The Slzf-31 Gene Decreases The Salt Stress Tolerance And Drought Tolerance Of Tomato

2021 ◽  
Author(s):  
Tong Pei ◽  
Yufang Bao ◽  
Tairu Wu ◽  
Ziyu Wang ◽  
Yue Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Virus Induced Gene Silencing ◽  
Chlorophyll Fluorescence Parameters ◽  
Tomato Plants ◽  
Drought And Salt Stress ◽  
Stem Bending ◽  
Physiological Indexes ◽  
Mda Content ◽  
Aba Content

Abstract The SlZF-31 gene is a member of the tomato C2H2 transcription factor family. Previous studies have shown that SlZF-31 gene expression is upregulated under drought stress and salt stress, but the specific function of this gene in tomato plants in response to these two kinds of stress is still unclear. To further explore the function of the SlZF-31 gene in tomato under drought stress and salt stress, we employed the virus-induced gene silencing (VIGS) method to reduce the expression of the SlZF-31 gene in tomato. The results showed that TRV2-SlZF-31 plants had higher levels of wilt and stem bending than CK and CK-TRV2 plants under drought and salt stress. The ABA content of TRV2-SlZF-31 plants were lower than those of CK and CK-TRV2 plants. The analysis of physiological indexes showed that the SOD and POD activity and the PRO content of TRV2-SlZF-31 plants were lower than those of CK and CK-TRV2 plants, while the MDA content of TRV2-SLlZF-31 plants was higher than those of CK and CK-TRV2 plants. The accumulation of H2O2 and O2- in TRV2-SlZF-31 plants was greater than those in CK and CK-TRV2 plants. The values of the chlorophyll fluorescence parameters (ΦII and qL) of TRV2-SlZF-31 plants were significantly lower than those of CK and CK-TRV2 plants. These results showed that the silencing of the SlZF-31 gene reduces the drought resistance and salt tolerance of tomato.

scholarly journals Characteristics of Azotobacter sp. strain AC11 and their effects on the growth of tomato seedlings under salt stress

2019 ◽  
pp. 520
Author(s):  
Honglian Ge
Keyword(s):  
Salt Stress ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Salt Resistance ◽  
Shoot Height ◽  
Tomato Seedlings ◽  
Pot Trials ◽  
Mda Content ◽  
Phosphorus And Potassium ◽  
Indole 3 Acetic Acid

This study was aimed to investigate the potential of Azotobacter sp. strain AC11 in promoting growth and enhancing resistance to salinity stress in tomato seedlings. In this study, we measured the ability of strain AC11 to fix nitrogen and solubilize phosphorus and potassium, as well as its production of indole-3-acetic acid (IAA) and siderophores. A greenhouse pot experiment was conducted to investigate whether strain AC11 promoted tomato seedlings’ growth and enhanced their salt resistance. The results showed that strain AC11 produced IAA and siderophores, fixed nitrogen, and solubilized potassium and phosphorus. In pot trials, strain AC11 increased the shoot height, root length, and dry and fresh weights of tomato seedlings, and also increased their chlorophyll, soluble protein, and soluble sugar content. Furthermore, the bacteria induced the activities of superoxide dismutase (SOD; EC: 1.15.1.1), peroxidase (POD; EC: 1.11.1.7), and catalase (CAT; EC: 1.11.1.6), while it reduced the malondialdehyde (MDA) content and rate of O2- generation in tomato seedlings under salt stress. In summary, Azotobacter sp. strain AC11 promoted the growth of tomato seedlings and induced resistance to salt stress by producing IAA and siderophores, promoting the activities of antioxidant enzymes, and increasing the content of osmotic adjustment substances as well as enhancing the availability of the macronutrients N, P, K, and Fe3+ in the soil.

scholarly journals Influence of Ascorbic Acid against Salt stress on the Germination, Biochemicals and Micronutrients Concentrations of Solanum melongena (L.)

2021 ◽  
Author(s):  
Muhammad Irfan ◽  
Gul Jan ◽  
Farzana Gul Jan ◽  
Waheed Murad
Keyword(s):  
Ascorbic Acid ◽  
Salt Stress ◽  
Biochemical Parameters ◽  
Ionic Composition ◽  
Chlorophyll Concentration ◽  
Solanum Melongena ◽  
Foliar Spray ◽  
Saline Control ◽  
Stress Concentrations ◽  
Negative Effects

The experiments were designed to know the function of ascorbic acid against salinity on germination, different biochemical and micronutrients concentrations of Solanum melongena. Ascorbic acid was applied as foliar spray on leaves and given in roots through irrigation at 100 and 200 ppm to the plants growed in non saline, 60mM and 100mM salt stress conditions. Different biochemical parameters i.e. chlorophyll, carotenoids, carbohydratrates, protein and micro nutrients concentrations of Solanum melongena were observed under different salt stress concentrations from control (non-saline), 60mM NaCl and 100mM NaCl solutions. The rates of different biochemical parameters and micro nutrients concentrations exhibited decrease in saline media in comparison with their respective control while ascorbic acid used as a foliar spray and through irrigation in roots at the concentration of 100 ppm and 200 ppm resulted promotion in non saline control as compared with salt concentrations media. It was determined that chlorophyll concentration was decreased considerably under salt stress. Amongst micro nutrients ionic composition of Na, K and Na/K ratio resulted that plants treated with different NaCl concentrations at 60mM NaCl and 100mM salt concentrations showed increase in Na and K ions and ascorbic acid were used as a foliar spray and in roots showed decrease in Na and K ions. Overall from the outcomes of the study it was observed that salinity stress significantly decrease vegetative and reproductive parameters that could be improved with the using of ascorbic acid. Best concentration found were 200 ppm ascorbic acid contributed towards an increase in growth irrespective of non-saline and saline conditions. Based on these findings, ascorbic acid treatment helps in alleviating the negative effects of salinity.

scholarly journals ANTIOXIDANT POTENTIAL OF TOMATO (SOLANUM LYCOPERSICUM L.) SEEDLINGS AS AFFECTED BY THE EXOGENOUS APPLICATION OF ORGANOIODINE COMPOUNDS

2020 ◽  
Vol 19 (2) ◽  
pp. 3-15
Author(s):  
Iwona Ledwożyw-Smoleń ◽  
Mariya Halka ◽  
Sylwester Smoleń ◽  
Michał Kruczek
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Salicylic Acid ◽  
Growth And Development ◽  
Higher Plants ◽  
Tomato Seedling ◽  
Tomato Seedlings ◽  
Solanum Lycopersicum L ◽  
Negative Effect ◽  
Modifying Effect

Salicylic acid is one of the regulatory compounds involved in numerous processes in plants. Previous studies indicated that also its halogen derivatives may exhibit similar roles. The aim of the work was to evaluate the influence of iododerivatives of salicylic acid such as: 5-iodosalicylc acid (5I-SA) and 3,5-diiodosalicylic acid (3,5diI-SA) on selected aspects of antioxidant capacity of tomato seedlings. The efficiency of improving iodine accumulation in tomato seedlings was also studied. No tested organoiodine compound had a negative effect on the growth and development of tomato seedlings. The presence of iodosalicylic acids in the nutrient solution led to a decrease of the content of salicylic acid, ascorbic acid and phenolic compounds in tomato seedlings. A modifying effect of tested organoiodine compounds on the antioxidant activity of tomato seedling extracts varied with respect to analyzed enzyme and applied assays. It has been confirmed that higher plants can take up and accumulate iodine from organoiodine compounds in levels not causing any symptoms of toxicity.

scholarly journals The Use of Halophytic Companion Plant (Portulaca oleracea L.) on Some Growth, Fruit, and Biochemical Parameters of Strawberry Plants under Salt Stress

Horticulturae ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 63
Author(s):  
Sema Karakas ◽  
Ibrahim Bolat ◽  
Murat Dikilitas
Keyword(s):  
Salt Stress ◽  
Biochemical Parameters ◽  
Dry Weight ◽  
Fruit Yield ◽  
Portulaca Oleracea ◽  
Soluble Solids ◽  
Average Weight ◽  
Chl A ◽  
Chlorophyll Contents ◽  
Companion Plant

Strawberry is a salt-sensitive plant adversely affected by slightly or moderately saline conditions. The growth, fruit, and biochemical parameters of strawberry plants grown under NaCl (0, 30, 60, and 90 mmol L−1) conditions with or without a halophytic companion plant (Portulaca oleracea L.) were elucidated in a pot experiment. Salt stress negatively affected the growth, physiological (stomatal conductance and electrolyte leakage), and biochemical parameters such as chlorophyll contents (chl-a and chl-b); proline, hydrogen peroxide, malondialdehyde, catalase, and peroxidase enzyme activities; total soluble solids; and lycopene and vitamin C contents, as well as the mineral uptake, of strawberry plants. The companionship of P. oleracea increased fresh weight, dry weight, and fruit average weight, as well as the total fruit yield of strawberry plants along with improvements of physiological and biochemical parameters. This study showed that the cultivation of P. oleracea with strawberry plants under salt stress conditions effectively increased strawberry fruit yield and quality. Therefore, we suggest that approaches towards the use of P. oleracea could be an environmentally friendly method that should be commonly practiced where salinity is of great concern.

scholarly journals The Use of Halophytic Companion Plant (Portulaca oleracea L.) on Some Growth, Fruit, and Biochemical Parameters of Strawberry Plants Under Salt Stress

2021 ◽  
Author(s):  
Sema Karakas ◽  
Ibrahim Bolat ◽  
Murat Dikilitas
Keyword(s):  
Salt Stress ◽  
Biochemical Parameters ◽  
Dry Weight ◽  
Fruit Yield ◽  
Portulaca Oleracea ◽  
Soluble Solids ◽  
Average Weight ◽  
Chl A ◽  
Chlorophyll Contents ◽  
Companion Plant

Strawberry is a saline sensitive plant adversely affected under slightly or moderately saline conditions. Growth and biochemical parameters of strawberry plants grown under NaCl (0-, 30-, 60-, and 90 mmol L-1) conditions with or without a halophytic companion plant (Portulaca oleracea L.) were investigated in a pot experiment. Salt stress negatively affected the growth, physiological (stomatal conductance, electrolyte leakage, total soluble solids) and biochemical parameters such as chlorophyll contents (chl-a and chl-b), proline, hydrogen peroxide, malondialdehyde, catalase, and peroxidase enzyme activities, lycopene, vitamin C contents along with the mineral uptake of strawberry plants. The companionship of P. oleacea increased fresh weight, dry weight, and fruit average weight and total fruit yield of strawberry plants along with the improvement of physiological and biochemical parameters. This study showed that cultivating of P. oleracea with strawberry plants under salt stress conditions effectively increased strawberry fruit yield and quality. We, therefore, that approaches towards the use of P. oleracea could be an environmentally friendly method that should be commonly practised where salinity is of great concern.

scholarly journals Engineered BcZAT12 gene mitigates salt stress in tomato seedlings

2021 ◽  
Vol 27 (3) ◽  
pp. 535-541
Author(s):  
Avinash Chandra Rai ◽  
Ashutosh Rai ◽  
Kavita Shah ◽  
Major Singh
Keyword(s):  
Salt Stress ◽  
Tomato Seedlings

scholarly journals Halotolerant Microbial Consortia for Sustainable Mitigation of Salinity Stress, Growth Promotion, and Mineral Uptake in Tomato Plants and Soil Nutrient Enrichment

2021 ◽  
Vol 13 (15) ◽  
pp. 8369
Author(s):  
Chintan Kapadia ◽  
R. Z. Sayyed ◽  
Hesham Ali El Enshasy ◽  
Harihar Vaidya ◽  
Deepshika Sharma ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Weight ◽  
Stress Factors ◽  
Microbial Consortia ◽  
Soil Conditions ◽  
Mineral Uptake ◽  
Shoot Height ◽  
Tomato Plants ◽  
Secondary Roots

Salinity significantly impacts the growth, development, and reproductive biology of various crops such as vegetables. The cultivable area is reduced due to the accumulation of salts and chemicals currently in use and is not amenable to a large extent to avoid such abiotic stress factors. The addition of microbes enriches the soil without any adverse effects. The effects of microbial consortia comprising Bacillus sp., Delftia sp., Enterobacter sp., Achromobacter sp., was evaluated on the growth and mineral uptake in tomatoes (Solanum Lycopersicum L.) under salt stress and normal soil conditions. Salinity treatments comprising Ec 0, 2, 5, and 8 dS/m were established by mixing soil with seawater until the desired Ec was achieved. The seedlings were transplanted in the pots of the respective pH and were inoculated with microbial consortia. After sufficient growth, these seedlings were transplanted in soil seedling trays. The measurement of soil minerals such as Na, K, Ca, Mg, Cu, Mn, and pH and the Ec were evaluated and compared with the control 0 days, 15 days, and 35 days after inoculation. The results were found to be non-significant for the soil parameters. In the uninoculated seedlings’ (control) seedling trays, salt treatment significantly affected leaf, shoot, root dry weight, shoot height, number of secondary roots, chlorophyll, and mineral contents. While bacterized seedlings sown under saline soil significantly increased leaf (105.17%), shoot (105.62%), root (109.06%) dry weight, leaf number (75.68%), shoot length (92.95%), root length (146.14%), secondary roots (91.23%), and chlorophyll content (−61.49%) as compared to the control (without consortia). The Na and K intake were higher even in the presence of the microbes, but the beneficial effect of the microbe helps plants sustain in the saline environment. The inoculation of microbial consortia produced more secondary roots, which accumulate more minerals and transport substances to the different parts of the plant; thus, it produced higher biomass and growth. Results of the present study revealed that the treatment with microbial consortia could alleviate the deleterious effects of salinity stress and improve the growth of tomato plants under salinity stress. Microbial consortia appear to be the best alternative and cost-effective and sustainable approach for managing soil salinity and improving plant growth under salt stress conditions.

scholarly journals Effect of Spaceflight on Tomato Seed Quality and Biochemical Characteristics of Mature Plants

Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 89
Author(s):  
Elena Dzhos ◽  
Nadezhda Golubkina ◽  
Marina Antoshkina ◽  
Irina Kondratyeva ◽  
Andrew Koshevarov ◽  
...  
Keyword(s):  
Seed Quality ◽  
Space Station ◽  
Field Conditions ◽  
Tomato Seed ◽  
Tomato Plants ◽  
Biochemical Characteristics ◽  
Tomato Seeds ◽  
Genetic Modifications ◽  
Total Antioxidant

Intensive space exploration includes profound investigations on the effect of weightlessness and cosmic radiation on plant growth and development. Tomato seeds are often used in such experiments though up to date the results have given rather vague information about biochemical changes in mature plants grown from seeds subjected to spaceflight. The effect of half a year of storage in the International Space Station (ISS) on tomato seeds (cultivar Podmoskovny ranny) was studied by analyzing the biochemical characteristics and mineral content of mature plants grown from these seeds both in greenhouse and field conditions. A significant increase was recorded in ascorbic acid, polyphenol and carotenoid contents, and total antioxidant activity (AOA), with higher changes in the field conditions compared to greenhouse. Contrary to control plants, the ones derived from space-stored seeds demonstrated a significant decrease in root AOA. The latter plants also showed a higher yield, but lower content of fruit dry matter, sugars, total dissolved solids and organic acids. The fruits of plants derived from space-stored seeds demonstrated decreased levels of Fe, Cu and taste index. The described results reflect the existence of oxidative stress in mature tomato plants as a long-term consequence of the effect of spaceflight on seed quality, whereas the higher yield may be attributed to genetic modifications.

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