CRISPR/Cas9 Trends and Future Scheme for Genome Engineering in Plants

The growing population meet the challenge for agricultural production. CRISPR/Cas9 technology is based on plant research for the development of the new varieties as well as disease resistance crops. In addition the deletion of significant characters makes the new alleles from the CRISPR/Cas9. Recent and reliable molecular scissor for genetic engineering. The review is focused on the various application of the CRISPR/Cas9 technology in plant enhancement of plant disease resistance, stress burden in plant, nutritional improvement, and quality of crops from the CRISPR/Cas9 system. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (cas9) is adopted from the prokaryotic type II system. CRISPR/Cas9 is simplicity and efficiency than ZENs and TALENs for the genome engineering. Due to rapid growing of the CRISPR/Cas9 system has been formulate the adaptation of many plant species. The current advancement of plants and future schemes of improve of CRISPR technology has been presented in contest of multiplex editing, knowledge on induced mutation whether the factor effect in CRISPR/Cas9 technology in plant. Remarkable perspective and challenges of CRISPR/Cas9 technology in significance of plant genetic modification.

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Versatile Applications of the CRISPR/Cas Toolkit in Plant Pathology and Disease Management

Phytopathology ◽

10.1094/phyto-08-20-0322-ia ◽

2020 ◽

Author(s):

Matthew Wheatley ◽

Yinong Yang

Keyword(s):

Plant Pathology ◽

Disease Resistance ◽

Disease Management ◽

Plant Disease Resistance ◽

Crop Production ◽

Genome Engineering ◽

Population Control ◽

Basic Research ◽

Gene Drive ◽

Basic Study

New tools and advanced technologies have played key roles in facilitating basic research in plant pathology and practical approaches for disease management and crop health. Recently, the CRISPR/Cas (clustered regularly interspersed short palindromic repeats/CRISPR associated) system has emerged as a powerful and versatile tool for genome editing and other molecular applications. This review aims to introduce and highlight the CRISPR/Cas toolkit and its current and future impact on plant pathology and disease management. We will cover the rapidly expanding horizon of various CRISPR/Cas applications in the basic study of plant-pathogen interactions, genome engineering of plant disease resistance, and molecular diagnosis of diverse pathogens. Using the citrus greening disease as an example, various CRISPR/Cas-enabled strategies are presented to precisely edit the host genome for disease resistance, to rapidly detect the pathogen for disease management, and to potentially use gene drive for insect population control. At the cutting edge of nucleic acid manipulation and detection, the CRISPR/Cas toolkit will accelerate plant breeding and reshape crop production and disease management as we face the challenges in 21st century agriculture.

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The Relationship between the Development of Human Capital and the Agricultural Economy in Rural Areas

Economy of agricultural and processing enterprises ◽

10.31442/0235-2494-2020-0-11-61-65 ◽

2020 ◽

pp. 61-65

Author(s):

B.A. Voronin ◽

◽

I.P. Chupina ◽

Ya.V. Voronina ◽

◽

...

Keyword(s):

Human Capital ◽

Rural Areas ◽

Agricultural Production ◽

Agricultural Sector ◽

Real Life ◽

Standard View ◽

Household Factors ◽

Economic Transformations ◽

The Relationship

The article discusses a non-standard view of the formation of human capital for work in organizations of the agricultural sector of the economy, in the context of modern socio-economic transformations. In the classical sense, human capital for agriculture should be formed and developed in rural areas. But in real life, this is not always the case, because there are many factors that prevent the classical solution of this problem. First, the demographic factor affects, second, social and household factors, and third, in many rural areas there are no working agricultural organizations where qualified agricultural specialists can work. All these and other circumstances actualize the problem of the quality of human capital in rural areas in relation to the development of agricultural production.

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Faculty Opinions recommendation of High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1003609.201391 ◽

2003 ◽

Author(s):

Jen Sheen

Keyword(s):

Disease Resistance ◽

Heat Shock ◽

Heat Shock Protein ◽

Gene Silencing ◽

High Throughput ◽

Plant Disease Resistance ◽

Plant Disease ◽

Heat Shock Protein 90 ◽

Virus Induced Gene Silencing

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Faculty Opinions recommendation of A gain-of-function mutation in a plant disease resistance gene leads to constitutive activation of downstream signal transduction pathways in suppressor of npr1-1, constitutive 1.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1016258.200354 ◽

2003 ◽

Author(s):

Laurence Rahme

Keyword(s):

Signal Transduction ◽

Disease Resistance ◽

Resistance Gene ◽

Plant Disease Resistance ◽

Plant Disease ◽

Signal Transduction Pathways ◽

Disease Resistance Gene ◽

Gain Of Function ◽

Constitutive Activation ◽

Plant Disease Resistance Gene

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EVALUATING THE CONTRIBUTION OF TOTAL FACTOR PRODUCTIVITY ON AGRICULTURAL GROWTH IN AN GIANG PROVINCE

Scientific Journal of Tra Vinh University ◽

10.35382/18594816.1.29.2018.30 ◽

2018 ◽

Vol 1 (29) ◽

pp. 29-37

Author(s):

Tan Van Truong

Keyword(s):

Total Factor Productivity ◽

Agricultural Production ◽

Science And Technology ◽

Agricultural Labor ◽

Factor Productivity ◽

Agricultural Growth ◽

Regression Approach ◽

Investment Capital ◽

Agricultural Demand

By the growth regression approach, the research has identified that the investment capital contributed 1,939 and agricultural labor contributed 1,291 to the agricultural growth of An Giang province. More specifically, the contribution of TFP (Total Factor Productivity) to the agricultural growth in the period 2000 - 2004 was averagely 0,11%, in 2005 - 2010 was -5,03%, and in period 2011 - 2016 was 0,81%. The total factor productivity contributed to the agricultural growth slowly. In order to raise the contribution of TFP, the research represents 05 solutions including the increase of the effectiveness of using the investment capital, the increase of the quality of labor, the application of the science and technology into agricultural production, agriculturalrestructuring, and the increase of agricultural demand.

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Transcriptomic Analyses on the Role of Nitric Oxide in Plant Disease Resistance

Current Issues in Molecular Biology ◽

10.21775/9781910190357.11 ◽

2016 ◽

Keyword(s):

Nitric Oxide ◽

Disease Resistance ◽

Plant Disease Resistance ◽

Plant Disease

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Breeding of Buckwheat for Usage of Sprout and Pre-Harvest Sprouting Resistance

Plants ◽

10.3390/plants10050997 ◽

2021 ◽

Vol 10 (5) ◽

pp. 997

Author(s):

Tatsuro Suzuki ◽

Takahiro Hara ◽

Kenjiro Katsu

Keyword(s):

Subsequent Growth ◽

Local Economies ◽

Cereal Grain ◽

The World ◽

New Varieties ◽

Sprouting Resistance ◽

Sprout Growth ◽

Noodle Quality ◽

Buckwheat Flour

Buckwheat is recognized as an important traditional crop and supports local economies in several regions around the world. Buckwheat is used, for example, as a cereal grain, noodle and bread. In addition, buckwheat is also used as a sprout or a young seedling. For these foods, sprouting is an important characteristic that affects food quality. For foods made from buckwheat flour, pre-harvest sprouting may decrease yield, which also leads to the deterioration of noodle quality. Breeding buckwheat that is resistant to pre-harvest sprouting is therefore required. Germination and subsequent growth are also important characteristics of the quality of sprouts. Although buckwheat sprouts are the focus because they contain many functional compounds, such as rutin, several problems have been noted, such as thin hypocotyls and husks remaining on sprouts. To date, several new varieties have been developed to resolve these quality issues. In this review, we summarize and introduce research on the breeding of buckwheat related to quality, sprouting and subsequent sprout growth.

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A family of pathogen-induced cysteine-rich transmembrane proteins is involved in plant disease resistance

Planta ◽

10.1007/s00425-021-03606-3 ◽

2021 ◽

Vol 253 (5) ◽

Author(s):

Marciel Pereira Mendes ◽

Richard Hickman ◽

Marcel C. Van Verk ◽

Nicole M. Nieuwendijk ◽

Anja Reinstädler ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane ◽

Disease Resistance ◽

Plant Disease Resistance ◽

Transmembrane Proteins ◽

Series Data ◽

Immune Gene ◽

Biological Processes ◽

Hypocotyl Growth ◽

Enhanced Resistance

Abstract Main conclusion Overexpression of pathogen-induced cysteine-rich transmembrane proteins (PCMs) in Arabidopsis thaliana enhances resistance against biotrophic pathogens and stimulates hypocotyl growth, suggesting a potential role for PCMs in connecting both biological processes. Abstract Plants possess a sophisticated immune system to protect themselves against pathogen attack. The defense hormone salicylic acid (SA) is an important player in the plant immune gene regulatory network. Using RNA-seq time series data of Arabidopsis thaliana leaves treated with SA, we identified a largely uncharacterized SA-responsive gene family of eight members that are all activated in response to various pathogens or their immune elicitors and encode small proteins with cysteine-rich transmembrane domains. Based on their nucleotide similarity and chromosomal position, the designated Pathogen-induced Cysteine-rich transMembrane protein (PCM) genes were subdivided into three subgroups consisting of PCM1-3 (subgroup I), PCM4-6 (subgroup II), and PCM7-8 (subgroup III). Of the PCM genes, only PCM4 (also known as PCC1) has previously been implicated in plant immunity. Transient expression assays in Nicotiana benthamiana indicated that most PCM proteins localize to the plasma membrane. Ectopic overexpression of the PCMs in Arabidopsis thaliana resulted in all eight cases in enhanced resistance against the biotrophic oomycete pathogen Hyaloperonospora arabidopsidis Noco2. Additionally, overexpression of PCM subgroup I genes conferred enhanced resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000. The PCM-overexpression lines were found to be also affected in the expression of genes related to light signaling and development, and accordingly, PCM-overexpressing seedlings displayed elongated hypocotyl growth. These results point to a function of PCMs in both disease resistance and photomorphogenesis, connecting both biological processes, possibly via effects on membrane structure or activity of interacting proteins at the plasma membrane.

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The Leucine-Rich Repeat Domain Can Determine Effective Interaction Between RPS2 and Other Host Factors in Arabidopsis RPS2-Mediated Disease Resistance

Genetics ◽

10.1093/genetics/158.1.439 ◽

2001 ◽

Vol 158 (1) ◽

pp. 439-450 ◽

Cited By ~ 4

Author(s):

Diya Banerjee ◽

Xiaochun Zhang ◽

Andrew F Bent

Keyword(s):

Disease Resistance ◽

Pseudomonas Syringae ◽

Plant Disease Resistance ◽

Effective Interaction ◽

Molecular Genetic ◽

Defense Responses ◽

Host Factors ◽

Leucine Rich Repeat ◽

Domain Swap ◽

Allele Specific

Abstract Like many other plant disease resistance genes, Arabidopsis thaliana RPS2 encodes a product with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. This study explored the hypothesized interaction of RPS2 with other host factors that may be required for perception of Pseudomonas syringae pathogens that express avrRpt2 and/or for the subsequent induction of plant defense responses. Crosses between Arabidopsis ecotypes Col-0 (resistant) and Po-1 (susceptible) revealed segregation of more than one gene that controls resistance to P. syringae that express avrRpt2. Many F2 and F3 progeny exhibited intermediate resistance phenotypes. In addition to RPS2, at least one additional genetic interval associated with this defense response was identified and mapped using quantitative genetic methods. Further genetic and molecular genetic complementation experiments with cloned RPS2 alleles revealed that the Po-1 allele of RPS2 can function in a Col-0 genetic background, but not in a Po-1 background. The other resistance-determining genes of Po-1 can function, however, as they successfully conferred resistance in combination with the Col-0 allele of RPS2. Domain-swap experiments revealed that in RPS2, a polymorphism at six amino acids in the LRR region is responsible for this allele-specific ability to function with other host factors.

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