Intravaginal probiotic therapy as a way to manage genital HPV infection in women

2021 ◽  
Vol 20 (3) ◽  
pp. 37-41
Author(s):  
V.V. Oleynik ◽  
◽  
E.A. Kremleva ◽  
A.V. Semenov ◽  
A.V. Sgibnev ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
Antiviral Treatment ◽  
Hpv Infection ◽  
The State ◽  
Female Reproductive Tract ◽  
Microbial Flora ◽  
Second Phase ◽  
Hpv Viral Load ◽  
Normal Microbial Flora

Objective. To study the possibility of managing human papillomavirus (HPV) infection using intravaginal probiotic therapy. Patients and methods. In the first phase, a retrospective analysis of outpatient medical records of 125 patients for 2 years was caried out to assess the HPV infection outcomes depending on the initial viral load and the state of normal microbial flora. In the second phase (prospective analysis), 43 HPV-positive patients with Lactobacillus deficiency who received (n = 20) or did not receive (n = 23) intravaginal probiotic were selected. The dynamics of HPV viral load were evaluated during the study. The dynamics of Lactobacillus abundance under the influence of probiotic in the groups with different HPV infection outcomes were analyzed. Results. The outcome of HPV infection with HPV viral load of ≤5 lg DNA copies/106 cells depended on the state of normal microbial flora; the value of normal microbial flora with HPV viral load of 5 lg DNA copies/106 cells was not significant. Intravaginal probiotic therapy in combination with antiviral drug in HPV-positive patients with Lactobacillus deficiency was more effective in improving the HPV infection outcomes. A decrease in viral load or HPV elimination was more likely to occur if normal microbial flora was restored as a result of probiotic therapy. Conclusion. Adding intravaginal probiotic therapy with Lactoginal® to antiviral treatment is an effective way to manage HPV infection in the female reproductive tract. Key words: human papillomavirus, vagina, lactobacilli, cervix, epithelial cells

scholarly journals Detection and estimation of human papillomavirus viral load in patients with cervical lesions

2014 ◽  
Vol 39 (2) ◽  
pp. 86-90 ◽  
Author(s):  
T Rahman ◽  
S Tabassum ◽  
M Jahan ◽  
A Nessa ◽  
Dr Ashrafunnessa
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
High Risk ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Promising Tool ◽  
High Risk Hpv ◽  
Hpv Viral Load

Human papillomavirus (HPV) high risk genotype infection and HPV viral load influences the development of invasive cervical cancer and cervical intra-epithelial neoplasia (CIN). HPV DNA testing for screening of cervical cancers may play a potential role in its early detection and management. The present study detected HPV DNA and estimated HPV viral load in different types of cervical lesions among Bangladeshi women. Using the Hybrid Capture 2 (HC2) assay, HPV DNA was tested among 68 women between 25-70 years of age. A total of 13 (19.1%) cases were positive for HPV DNA. The highest viral load (501 x 10³ copies/ml) was detected in a patient with invasive carcinoma, while the lowest viral load (105 x 10³ copies/ml) was detected from a case of chronic cervicitis. The mean viral load in CIN I was 119.25 x 10³±12.5 x 10³ copies/ml (range: 110 x 10³ - 137 x 10³) and 208.50 x 10³ ± 0.59 x 10³ copies/ml (range: 139 x 10³-305 x 10³) in CIN II / III. Interestingly, HPV DNA was detected from a patient with normal cytological findings. Our study observed a moderate presence of high-risk HPV genotypes among women with cervical lesions. The HPV viral load varied with the age of the patients and stage of cervical lesions. The HC2 assay is a promising tool for diagnosing high-risk HPV infection especially before cytology tests show any abnormality. DOI: http://dx.doi.org/10.3329/bmrcb.v39i2.19648 Bangladesh Med Res Counc Bull 2013; 39: 86-90

scholarly journals Acquisition and Persistence of Human Papillomavirus 16 (HPV-16) and HPV-18 Among Men With High-HPV Viral Load Infections in a Circumcision Trial in Kisumu, Kenya

2014 ◽  
Vol 211 (5) ◽  
pp. 811-820 ◽  
Author(s):  
Virginia Senkomago ◽  
Danielle M. Backes ◽  
Michael G. Hudgens ◽  
Charles Poole ◽  
Kawango Agot ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
Hpv 16 ◽  
Human Papillomavirus 16 ◽  
Hpv Viral Load ◽  
Hpv 18

scholarly journals Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

2020 ◽  
Author(s):  
Xuerong Lu ◽  
Tiantian Wang ◽  
Youzhong Zhang ◽  
Yuzhen Liu
Keyword(s):  
Multivariate Analysis ◽  
Human Papillomavirus ◽  
Viral Load ◽  
High Risk ◽  
Influencing Factors ◽  
Cervical Screening ◽  
Cervical Lesion ◽  
Cervical Lesions ◽  
Hpv Types ◽  
Hpv Viral Load

Abstract Background: High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and relationship between viral load and degree of cervical lesion is controversial. This study was to explore possible influencing factors of HR-HPV viral load in uterine cervix.Methods: 605 women who needed colposcopic evaluation for abnormal cervical screening in Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from endo- and ectocervix separately using two different cervical brushes. Hybrid Capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, area and location of cervical lesions were recorded. The correlation between viral load and influencing factors was analysed using univariate and multivariate analysis.Results: HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P<0.05). Viral load with combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P<0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with combination of endo- and ectocervical sampling (P<0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P<0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P>0.05).Conclusion: HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

scholarly journals Human Papillomavirus Viral Load and Transmission in Young, Recently Formed Heterosexual Couples

2019 ◽  
Vol 220 (7) ◽  
pp. 1152-1161 ◽  
Author(s):  
Michel D Wissing ◽  
Karolina Louvanto ◽  
Emilie Comète ◽  
Ann N Burchell ◽  
Mariam El-Zein ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
Sexually Active ◽  
Sex Partners ◽  
Heterosexual Couples ◽  
Hpv Dna ◽  
Logistic Regression Models ◽  
Hpv Prevalence ◽  
Polymerase Chain ◽  
Hpv Viral Load

Abstract Background We studied the association between human papillomavirus (HPV) viral load (VL) and HPV concordance. Methods The HITCH cohort study included young, heterosexual, recently formed, sexually active couples. Questionnaires and genital samples were collected at 0 and 4 months. Samples were tested for HPV DNA by polymerase chain reaction (PCR; Linear Array). VLs of HPV6/11/16/18/31/42/51 were quantified using type-specific real-time PCR. Correlations between VL and type-specific HPV prevalence and incidence were evaluated using multilevel, mixed-effects linear/logistic regression models. Results  We included 492 couples. VLs were higher in penile than vaginal samples. VL at subsequent visits correlated significantly within men (r, 0.373), within women (r, 0.193), and within couples (r range: 0.303–0.328). Men with high VL had more type-specific persistent HPV infections (odds ratio [OR], 4.6 [95% confidence interval {CI}, 2.0–10.5]). High VL in men was associated with prevalent (OR, 5.3 [95% CI, 2.5–11.2]) and incident (OR, 6.7 [95% CI, 1.5–30.7]) type-specific HPV infections in their partner. Women’s VL was associated with type-specific HPV prevalence in their partner at the same (OR, 5.9) and subsequent (OR, 4.7) visit. Conclusions Persistent HPV infections have limited VL fluctuations. VL between sex partners are correlated and seem predictive of transmission episodes.

scholarly journals Increased High-Risk Human Papillomavirus Viral Load Is Associated With Immunosuppressed Microenvironment and Predicts a Worse Long-Term Survival in Cervical Cancer Patients

2019 ◽  
Vol 153 (4) ◽  
pp. 502-512 ◽  
Author(s):  
Meng Cao ◽  
Ying Wang ◽  
Depu Wang ◽  
Yixin Duan ◽  
Wei Hong ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Viral Load ◽  
High Risk ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Immune Microenvironment ◽  
Term Survival ◽  
Cox Regression Analysis ◽  
Hpv Viral Load

Abstract Objectives To evaluate the correlation between tumor-infiltrating lymphocytes (TILs) and the viral load of high-risk human papillomavirus (HR-HPV) in cervical cancer patients. Methods A total of 62 cervical cancer patients were recruited during 1993-1994 and assigned into four groups treated with radiotherapy alone or radiotherapy combined with chemotherapy and/or thermotherapy. Ki67+ tumor cells, CD4+, CD8+, FoxP3+, OX40+ and granzyme B+ TILs were detected by immunohistochemistry. The viral load of HR-HPV in biopsy tissues before therapy was detected by in situ hybridization. Results The patients with high HPV viral load showed a significantly lower 15-year survival rate and an advanced International Federation of Gynecology and Obstetrics (FIGO) stage and increased recurrence rate. The distribution of Ki67+ tumor cells, FoxP3+ TILs, and CD8+/FoxP3+ ratio was obviously different between low and high HPV viral load groups. A worse clinical outcome was also implicated with increased HPV viral load tested by Cox regression analysis. Conclusions Patients with increased HR-HPV viral load tend to be resistant to therapy with decreased immune surveillance in the immune microenvironment. Thus, HR-HPV viral load would influence the local immune microenvironment, and then further affect the survival of cervical cancer patients.

scholarly journals Prognostic Impact of Human Papillomavirus Infection on Cervical Dysplasia, Cancer, and Patient Survival in Saudi Arabia: A 10-Year Retrospective Analysis

2020 ◽  
Author(s):  
F.S. Alhamlan ◽  
D.A. Obeid ◽  
H.H. Khayat ◽  
A.M. Tulbah ◽  
I.A. Al-Badawi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Saudi Arabia ◽  
Human Papillomavirus ◽  
Viral Load ◽  
Cox Regression ◽  
Survival Rates ◽  
Hpv Infection ◽  
Prognostic Impact ◽  
Screening Programs ◽  
Hpv Status

AbstractCervical cancer is caused by persistent human papillomavirus (HPV) infection. However, HPV prevalence data and survival rates among HPV-infected women are scare in Saudi Arabia. This study assessed the prevalence of HPV genotypes in a 10 year time-frame. Cervical biopsy specimens underwent HPV detection, HPV viral load using qPCR, HPV genotyping, p16INK4a expression measurement using immunohistochemistry. Kaplan-Meier plots were constructed to analyze overall survival rates. Of the 316 cervical specimens examined, HPV was detected in 96 (30.4%); 37.3% had cervical cancer; 14.2% cervical intraepithelial neoplasia (CIN) III, 4.1% CIN II, and 17.0% CIN I. A significant association was found between HPV-16 viral load and disease progression (P < .001, Mann-Whitney U) and between HPV presence and cervical cancer (χ2, 56.78; P < .001). The expression of p16INK4a was a significant predictor of survival: women who had p16INK4a overexpression had poorer survival rates (multivariate Cox regression, hazard ratio, 3.2; 95% CI, 1.1-8.8). In addition, multivariate models with HPV status and cervical cancer diagnosis showed that HPV status was a significant predictor of survival: HPV-positive women had better survival rates than HPV-negative women (haza. These findings suggest that implementing cervical cancer and HPV screening programs may decrease cervical cancer rates and improve survival rates of women in Saudi Arabia.

scholarly journals The Efficacy of Human Papillomavirus Elimination during Treatment of Cervical Disorders

Doctor Ru ◽  
2020 ◽  
Vol 19 (8) ◽  
pp. 80-85
Author(s):  
O.P. Vinogradova ◽  
◽  
O.I. Artemova ◽  
◽  
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
Study Design ◽  
Direct Relationship ◽  
Caspase 3 ◽  
Antiviral Treatment ◽  
Caspase 9 ◽  
Group I ◽  
Hpv Test ◽  
Study Results

Study Objective: To assess the efficacy of antiviral medications, using the apoptotic markers caspase-3 and caspase-9, in the treatment of cervical disorders accompanied by papillomavirus infection. Study Design: This was a prospective study. Materials and Methods: One hundred and seventy women with a positive human papillomavirus (HPV) test, aged 18 to 45, participated in the study. Group I consisted of 60 women with histologically confirmed cervical leukoplakia; Group II was made up of 55 patients with cervical intraepithelial neoplasia (CIN) I; and Group III comprised 55 women with CIN II. The control group consisted of 40 healthy women. All participants underwent a standard examination and had measurements taken for levels of caspase-1, caspase-3, and caspase-9, which were used to assess changes in their apoptotic programs. During the study, the patients with HPV were divided into subgroups. In the cervical leukoplakia group, 34 women (subgroup Ia) were only monitored and 26 women (subgroup Ib) received antiviral treatment. Similarly, in the CIN I group, 27 patients (subgroup IIa) were only monitored and 28 women (subgroup IIb) received antiviral drugs. In the CIN II group, all patients underwent excision of their lesions. According to the study design, 28 women who had undergone excision had monitoring alone (subgroup IIIa) and for 27 participants the surgical treatment was combined with an antiviral medication (subgroup IIIb). The efficacy of the treatment regimens was assessed three and six later by cytology and colposcopy data and levels of caspase-3 and caspase-9. Treatment efficacy was assessed chiefly by the presence or absence of HPV or reduction in viral load below significant levels. Study Results: After three and six months, viral load reduction in all subgroups of women who received antiviral treatment was greater and more of these women had a negative HPV test (after three and six months the overall efficacy was 11.7% vs. 30.7% and 20.5% vs. 42.3% in subgroups Ia and Ib, respectively; 7.4% vs. 21.4% and 22.2% vs. 35.7% in subgroups IIa and IIb, respectively; and 75% vs. 85.2% and 64.2% vs. 92.6% in subgroups IIIa and IIIb, respectively). Caspase-3 and caspase-9 levels decreased in both the subgroups that had monitoring alone and the subgroups that received combination treatment, but in the latter case they were closer to the control group’s levels. Correlation analysis revealed a strong direct relationship between clinically significant viral load and caspase-3 levels (rху = 0.79) and a moderate direct relationship between caspase-9 levels and high viral load (rху = 0.58). Conclusion: The proposed comprehensive approach, including antiviral therapy, to the treatment of HPV-associated cervical disorders such as leukoplakia, CIN I and CIN II, has higher clinical efficacy than monitoring over time alone or only the excision of lesions (in patients with CIN II). This is because of its effects on the system of natural killers and stimulation of the ability of cytotoxic lymphocytes to identify and destroy defective cells. Keywords: human papillomavirus, apoptosis, caspases, dysplasia, leukoplakia.

scholarly journals Association Between Promoter Polymorphisms of IL-1Β, IL-4 and IL-6 Genes and a Viral Load Infected Women with Human Papillomavirus

2021 ◽  
Author(s):  
Abbas Hadi Albosale ◽  
Elena Vladimirovna Mashkina
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
Gene Interactions ◽  
Control Group ◽  
Case Group ◽  
Single Individual ◽  
Promoter Polymorphisms ◽  
Individual Snps ◽  
Significant Difference ◽  
Hpv Viral Load

Background: The purpose of this study was to investigate the association between IL-4 -589C>T, IL-6 -174G>C, IL-1β -31T>C and IL-1β -511C>T genes polymorphism with high concentrations of human papillomavirus (HPV), and the influence of gene-gene interactions on persistent human papillomavirus infection. Methods: In this study, 101 infected women with high HPV viral load and 93 healthy women were involved in a case-control study. Genotyping of SNPs for IL-4 -589C>T, IL-6 -174G>C, IL-1β -31T>C and IL-1β -511C>T genes was carried out by allele-specific PCR. Quantitative analysis of HPV-DNA was performed by Amplisens HPV HCR genotype-titer software. Gene-gene interactions were analyzed using multifactor dimensionality reduction (MDR) algorithm. Haplotype interactions were analyzed by HaploView 4.2 tool. Results: The study of single individual SNPs in promoters of IL-4 -589C>T, IL-6 -174G>C and IL-1β -31T>C genes did not reveal statistically significant difference in genotypesand allele frequencies among women with high HPV viral load and control group. The frequency of -511T allele and TT genotype of the IL-1β gene in case group was significantly higher than the one in control group (OR=1.71, p=0.012 and OR=2.02, p=0.046, respectively). Haplotype analysis revealed that -511C/-31T haplotype for IL-1β gene is significantly less common among women with high HPV viral load  (p=0.018). Conclusion: The haplotype -511C/-31T for IL-1β gene is associated with a protective effect against increasing HPV viral load. The frequencies of -511T allele and -511TT genotype of the IL-1β -511C>T were significantly higher among women with HPV in comparison to control group.

The ways of reducing diagnostic and therapeutic aggression of the patients with HPV-infection in reproductive age

2017 ◽  
pp. 51-58
Author(s):  
N.M. Voloshena ◽  
◽  
E.D. Zvantseva ◽  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Viral Load ◽  
Bacterial Infections ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Cervical Neoplasia ◽  
Reproductive Age ◽  
Etiologic Factor ◽  
Sexually Transmitted

The problem of early diagnostics and prevention of cervical cancer is actual in Ukraine.The leading etiologic factor in the genesis of cervical neoplasia and a number of other organs is Human papillomavirus (HPV). The human papillomavirus is sexually transmitted and has high contagiosity. Cancer prevention consists in effective screening, early detection and treatment of pathological changes in the cervix. The aggressive treatment of diseases caused by (HPV) has been replaced by a tactic of a differentiated approach, taking into account to the age of the woman and her reproductive plans. The objective: was to study the efficacy and tolerability of the combined use of Proteflazid® systemically in drops form and locally in the form of suppositories for 3 months in patients with cervical intraepithelial neoplasia (CIN) of lung and moderate severity (CIN 1 and CIN 2) associated with the human papillomavirus (HPV); determination on the basis of the results of the need for further destructive treatment. Materials and methods. For the period from July 2016 to September 2017, we examined and treated 86 women with morphologically confirmed intraepithelial neoplasia of the cervix associated with HPV infection. Results. Based on the performed studies, it was found that 6 months after treatment with Proteplasid® systemically and locally for 3 months, regression of CIN was noted in 93% of patients. In all cases, a reduction in viral load of more than 2 Lg of HPV/105, which is a marker of the effectiveness of antiviral therapy, has been recorded. Six months after treatment in 84% of patients and 9 months in 88%, there was complete elimination of HPV or reduced viral load to clinically insignificant values – less than 3 Lg. Conclusion. The drug Proteflazid® suppositories and drops contributes to the elimination of human papillomavirus (HPV) and other viral-bacterial infections and reduces the risk of cervical neoplasia. Key words: cervical cancer, screening, cervical neoplasia, Human papillomavirus, viral-bacterial infections, Proteflazid®.

Sign in / Sign up

Export Citation Format

Share Document