scholarly journals RESPON AKTIVITAS TIKUS WISTAR JANTAN AKIBAT KONDISI DIET TINGGI SUKROSA DIUKUR MENGGUNAKAN PEREKAM AKTIVITAS

Jurnal BIOMA ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 104
Author(s):  
Yanuar Restu Wijaya ◽  
Koekoeh Santoso ◽  
Isdoni Isdoni ◽  
Atin Supiyani
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Feeding Activity ◽  
Control Group ◽  
High Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Track System ◽  
Male Wistar Rats ◽  
Auto Track

Excessive energi consumption play a role in increasing blood glucose levels (hyperglycemia), due to the inability of the hormone insulin to compensate the high blood glucose levels. Blood glucose receptor found in the hypothalamus of VMH (Ventro Medial Hypothalamus) and LH (the Lateral Hypothalamus) is able to detect and change the settings for the feeding activity. The aim of this study was to obtain the relationship between high sucrose feeding on male Wistar rats of behavior and activity. This method uses Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% sucrose, the second group was given additional feed 40% sucrose, a third group was given additional feed 60% sucrose, and the last as a control group. This feed given continuously for 70 days.Observation of activities conducted using Opto-varimex® auto-track system ver.4.31. The results showed an corellation between activity based doses of sucrose.

scholarly journals PENGAMATAN AKTIVITAS TIKUS WISTAR JANTAN DENGAN ALAT OPTO- VARIMEX® PADA KONDISI DIET TINGGI MINYAK TRANS

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 89
Author(s):  
NURUL MASYITA ◽  
KOEKOEH SANTOSO ◽  
NASTITI KUSUMORINI ◽  
ARYANI SISMIN SATYANINGTIJAS ◽  
ATIN SUPIYANI
Keyword(s):  
Adipose Tissue ◽  
Wistar Rats ◽  
Feeding Activity ◽  
Wistar Rat ◽  
Control Group ◽  
Trans Fat ◽  
Treatment Groups ◽  
Track System ◽  
Male Wistar Rats ◽  
Auto Track

ABSTRACT Excessive energi consumption of high-fat diet increase the body’s adipose tissue which also affect the increased secretion of leptin, this is because leptin secretion is proportional to the mass of adipose tissue. Leptin activates the lateral hypothalamus as the center of hunger and feeding activity. The aim of this study was to obtain the relationship between high trans fat feeding on male Wistar rats and feeding activity. This method uses male Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% trans fat, the second group was given additional feed 40% trans fat, a third group was given additional feed 60% trans fat, and the last as a control group. This feed was given once a day for 70 days. Observation of activities perform using Opto-varimex® auto-track system ver. 4.31. The results showed an corellation between activity based doses of trans fat. Keywords: Opto-varimex®, Trans fat, Activity, Wistar rat.

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

scholarly journals THE EFFECTS OF CORN FLOUR WITH TEMPEH FLOUR SUPPLEMENTATION FEEDING IN DIABETES MELLITUS WISTAR RATS TOWARD BLOOD GLUCOSE LEVEL

2017 ◽  
Vol 4 (2) ◽  
pp. 24
Author(s):  
Fatifa Asmarani ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Wistar Rats ◽  
Control Group ◽  
Standard Diet ◽  
Corn Flour ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Background: Diabetes mellitus (DM) is metabolic disorder syndrome characterized by hyperglycemia due to deficiency of insulin secretion. Patients of diabetic patients increases each year, so it needs proper handling. Corn flour is a source of carbohydrates with a low glycemic index to help reduce the rise in blood sugar levels. In addition, supplementation with tempeh flour containing isoflavones act to protect cells from free radical thereby inhibiting damage cells. Fiber in tempeh affect blood glucose levels because it slows the absorption of glucose.Objective: To determine the effect supplementation of corn flour with tempeh flour on blood sugar levels in diabetes mellitus Wistar rats.Methods: The study was true experimental design with Pre-posttest control group design, samples were 20 male Wistar rats aged 2-3 months were then divided into 4 groups (T0, T1, T2, T3.) T0 was injected with alloxan as 30 mg / 150 grams of BB rats are subsequently treated with standard diet; T1 was dministered by standard diet 50% + 50% corn flour; T2 was administered by standard diet 50% + 30% + corn flour tempeh flour 20%; and T3 was administered by standard diet of corn flour 50% + 15% + 35% tempeh flour for 2 weeks. Blood glucose levels were measured with a glucose kit (GOD FS). Research data were analyzed by One Way ANOVA followed by Tukey's Post Hoc test p-value <0.05.Results: There were significant differences on blood sugar levels in all groups (p = 0.000): control group (only dietary standards), T1 (administered by standard diet 50% + corn flour 50%), T2 (administered by a standard diet 50% + cornstarch 30% tempeh flour + 20%) and T3 (administered by standard diet of corn flour 50% + 15% + 35% tempeh flour).Conclusion: corn flour with tempeh flour supplementation effect on decresing of blood sugar levels.

scholarly journals Red dragon fruit (Hylocereus spp.) peel marmalade effectively improve blood glucose and lipid profile of hypercholesterolemic wistar rats

2020 ◽  
Vol 9 (1) ◽  
pp. 61-67
Author(s):  
Natalia Desy Putriningtyas ◽  
Intan Permatasari ◽  
Dita Oktaviani ◽  
Anastasia Servia Raha ◽  
Siti Wahyuningsih
Keyword(s):  
Blood Glucose ◽  
Functional Food ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Fruit Peel ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Dragon Fruit ◽  
Significant Difference

Background: Polyphenols, antioxidants, dietary fiber, and vitamin contained in the red dragon fruit peel. Red dragon fruit peel can be processed into marmalade. Red dragon fruit peel marmalade has the potential to be a functional food. Functional food is food that has a physiological function based on scientific studies.Objectives: The objective of this study is to analyze the effect of red dragon fruit peel marmalade on fasting blood glucose levels, HDL, LDL, and triglycerides levels of hypercholesterolemic Wistar rats.Methods: This study used a pre-&post-test control group design. Hypercholesterolemic male Wistar rats were randomly assigned into five groups. Hypercholesterolemia was induced by 1% cholesterol powder and 0.5% cholic acid for two weeks. All groups received standard chow. Samples were grouped into five groups: K-; K+; K1 (0.94 g/kg b.wt/day); K2 (1.41 g/kg b.wt/day); K3 (1.88 g/kg b.wt/day). The intervention was carried out for 28 days. GDP level was measured using the GOD-PAP. HDL, LDL, and triglyceride were analyzed with spectrophotometry. GDP, HDL, LDL, and triglyceride levels were measured twice before fasting. A paired t-test and one-way ANOVA were used to analyze the data.Results: The result showed that K-; K1; K2; K3 had a significant difference between groups before and after the intervention (p<0.05). Red dragon fruit peel Marmalade was able to reduce the levels of GDP, LDL, triglycerides, and increase HDL (p <0.05).Conclusion: Red dragon fruit peel marmalade reduced fasting blood glucose levels, LDL, triglyceride levels, and increased HDL levels of hypercholesterolemic Wistar rats.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE DENGAN MENGGUNAKAN ELISA READER

2020 ◽  
Vol 7 (2) ◽  
pp. 1-5
Author(s):  
Sukmawati Syarif ◽  
Nurnaningsih Nurnaningsih ◽  
Mamat Pratama
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sample Extract ◽  
Male Wistar Rats ◽  
Muntingia Calabura

Kersen leaves (Muntingia calabura L) contain metabolites such as alkaloids, anthrax, polyphenols, tannins, saponins and are rich in flavonoid components such as flavones, flavonones, flavan and biflavan which have antidiabetic activity. According to a study conducted by Apriyanti (2016), the ethanol extract of kersen (Muntingia calabura L) at a dose of 250 mg / kg BB significantly reduced blood glucose levels in male wistar rats. In this study aimed at determining IC50 ethanol extract of Muntingia calabura L as an inhibitor of α-glucosidase enzymes using ELISA reader. The method was divided into 3 category is 1 (sample extract), 2 (blank) and 3 (akarbose). Each group added 25 µL α-glucosidase solution (0.25 units / mL then measured using ELISA reader 405 nm. The results showed that the ethanol extract of kersen (Muntingia calabura L) had activity as an inhibitor of α-glucosidase enzyme with IC50 34,197 µg / mL and can be categorized as active.

scholarly journals Uji efek ekstrak buah okra (Abelmoschus esculentus) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Leonardus B.A. Prakoso ◽  
Christi Mambo ◽  
Mona P. Wowor
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Abelmoschus Esculentus ◽  
Control Group ◽  
Elevated Blood ◽  
Glucose Levels ◽  
Elevated Blood Glucose ◽  
Blood Glucose Levels

Abstract: Okra (Abelmoschus esculentus) is one of many herbal medicine that have been used worldwide to prevent and treat diabetes mellitus. This research aimed to evaluate the effect of garlic bulbs extract administration on elevated blood glucose levels on Wistar rats (R. norvegicus) induced by alloxan. The subject in this research were male Wistar rats with total of 15 samples divided into 5 groups, consisting of one negative control group, one positive control group who were induce by 130 mg/kgBW of alloxan that cause the Rats in conditions of hyperglycemic, and three groups of Rats were given okra extract with dose of 0.375 ml/100 gBW of rat, 0.75 ml/100 gBW of rat , and 1.5 ml/100 gBW of rat. Blood glucose levels was measured on day zero, day one and day two every thirty minutes on 0, 30, 60, 90, 120, and every six hours on 0, 6, 12, 18, and 24. Data from the measurement showed that 0.375 ml/100 gBW, 0.75 ml/100 gBW , and 1.5 ml/100 gBW of okra extract can’t reduce elevated blood glucose levels on Wistar rats.Keywords: Abelmoschus esculentus, okra, blood glucose levels, alloxan Abstrak: Buah Okra (Abelmoschus esculentus) diyakini masyarakat sebagai salah satu tanaman obat yang mampu mencegah dan mengobati penyakit diabetes. Penelitian ini bertujuan untuk menguji apakah pemberian ekstrak buah okra dapat menurunkan kadar glukosa darah tikus Wistar (R. norvegicus) yang diinduksi aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok, terdiri atas satu kelompok kontrol negatif, satu kelompok kontrol positif yang diberi aloksan dengan dosis 130 mg/kgBB yang menyebabkan tikus Wistar dalam keadaan hiperglikemik yang kemudian diinsuksi insulin, dan tiga kelompok Tikus hiperglikemik diberi ekstrak buah okra dengan dosis 0,375 ml/100 gBB tikus, 0,75 ml/100 gBB tikus, dan 1,5 ml/100 gBB tikus. Data diperoleh dari pemeriksaan kadar glukosa darah pada semua kelompok tikus Wistar pada hari ke nol, pertama, dan kedua pada menit ke-0, 30, 60, 90, 120 dan jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak buah okra dengan dosis 0,375 ml/100 gBB tikus, 0,75 ml/100 gBB tikus, dan 1,5 ml/100 gBB tikus tidak mempunyai efek menurunkan kadar glukosa darah tikus Wistar. Kata kunci: abelmoschus esculentus, buah okra, kadar glukosa darah, aloksan

scholarly journals Antihyperglycemic effect of turmeric aqueous root extract and morphological changes in the pancreas of alloxan-induced hyperglycemic Wistar rats

2021 ◽  
Vol 11 (5-S) ◽  
pp. 19-24
Author(s):  
Samuel I. Ogenyi ◽  
Ezinne Imoko ◽  
Anthony A. Ngokere ◽  
Amalachukwu O. Ike
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Control Group ◽  
Root Extract ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Body Weights ◽  
Group A ◽  
Group B

Introduction: The study aims to evaluate the anti-hyperglycaemic effect of turmeric and the histological changes in the pancreas of adult Wistar rats. Materials and Methods: Forty adult Wistar rats were divided into four groups of [A-D] ten rats per group. Group A was normal control, group B was diabetic control, group C was treated with 300mg/kg weight of extract and group D was 500mg/kg body weight. All animals had access to food and water ad libitum.  Aqueous extract of turmeric was administered orally, once daily for 14 days. The rats were then anaesthetized with dichloromethane before sacrifice and sample collection. Pancreatic tissues were processed, stained using the hematoxylin and eosin (H&E) method. Blood glucose estimation was determined using the glucose oxidase method. Results: The mean body weights of the rats across groups were not statistically significant. Similarly, blood glucose levels across groups revealed a progressive statistically significant increase from group A to D and from day one to day 17. Pairwise comparison of group A with B, C and D showed a statistically significant increase but a comparison of Group B with C and D were not significant. Discussion: There was progressive body weight increase of the rats from day one to the final day across all groups and reduction in the blood glucose levels of the animals treated with 300mg/kg and 500mg/kg body weights, after seven days of treatment, but these, progressively increased with time even with treatment. The overall pancreas features indicate moderate inflammatory changes. Keywords: turmeric, anti- hyperglycemia, alloxan, Wistar rats

scholarly journals ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSA

2013 ◽  
Vol 13 (2) ◽  
pp. 116 ◽  
Author(s):  
Nafila Salma ◽  
Jessy Paendong ◽  
Lidya I Momuat ◽  
Sariyana Togubu
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Blood Sugar ◽  
Plant Extract ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats

ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSAABSTRAK Telah dilakukan penelitian untuk menentukan dosis optimum dari ekstrak tumbuhan suruhan (Peperomia pellucida [L.] Kunth) sebagai antihiperglikemik tikus wistar yang diinduksi sukrosa. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan tikus putih jantan wistar berjumlah 20 ekor tikus yang dibagi dalam 5 kelompok perlakuan yaitu kelompok perlakuan kontrol negatif (K-) diberi CMC 0,5%, kelompok perlakuan kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok perlakuan ekstrak tumbuhan suruhan  dosis 20; 40; 80 mg/kgBB. Data diperoleh dari pemeriksaan kadar gula darah puasa (basal), 30 menit setelah diindukasi sukrosa dan pada menit ke 30, 60, dan 120 setelah diinduksi sediaan per oral. Data diolah dengan uji ANOVA dan Ducan untuk membandingkan antara kelompok kontrol negatif, ekstrak dan kontrol positif. Data hasil pengujian kadar glukosa darah dengan dosis 20; 40; 80 mg/kgBB memberikan penurunan kadar glukosa darah. Pemberian ekstrak tumbuhan suruhan dengan dosis 40 mg/kgBB pada menit ke-120 memberikan hasil  yang signifikan dan bebeda nyata dibandingkan kelompok kontrol negatif (CMC 0,5%) dan tidak berbeda nyata dengan glibenklamid dosis 0,45 mg/kgBB. Kesimpulannya pemberian ekstrak tumbuhan suruhan dosis 40 mg/kgBB pada menit ke 120  memberikan penurunan kadar glukosa darah yang efektif dan berbeda nyata (signifikan) dibandingkan pemberian ekstrak tumbuhan suruhan dosis 20 dan 80 mg/kgBB. Kata Kunci : Peperomia pellucida [L.] Kunth, Tumbuhan Suruhan, Kadar Glukosa Darah, Sukrosa. ANTIHYPERGLYCEMIC FROM SURUHAN PLANT (Peperomia pellucida [L.] Kunth) EXTRACT AGAINST WISTAR RATS (Rattus norvegicus L.) WITH INCLUDED BY SUCROSE ABSTRACT The purpose of this research is to determine optimum dose of Suruhan (Peperomia pellucida [L.] Kunth) extract as Antihyperglycemic agent against Wistar Rats with Induced by Sucrose. This research using glucose tolerance test methods. Used 20 male Wistar rats were divided into 5 groups, they are the negative control treatment group (K-) were given 0.5% CMC, the positive control groups (K+) given 0,45 mg/kgBB  Glibenklamid, and Suruhan extract treatment group of 20; 40; 80 mg/kgBB dose. Data obtained from the examination offasting blood sugar (basal) content, 30 minutes after induced bysucroseandat minute of 30, 60, and120 after-induced oraldosage.The data were processed by ANAVA and LSD testtocompare betweenthe negativecontrol group, the extractandpositive control. Dataresults of testing blood sugar levels with doses of 20, 40, 80 mg/kgBB gavea decreasein blood glucose levels were significant and significantly different than the negative control group (CMC 0.5%) and did not give a clear difference with 0.45 mg/kgBB dose of Glibenclamide. Agents of plant extract at a dose of 40 mg/kgBB body weight gives better results to the decline in blood glucose levels compared with doses of 20 and 80 mg/kgBB. In conclusion, Suruhan plant extract has an effect on blood glucose levels decreased against white male Wistar rats were induced sucrose. Keywords : Peperomia pellucida [L.] Kunth, Suruhan Plant, Blood Glucose Levels, Sucrose

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