scholarly journals Uji efek ekstrak buah okra (Abelmoschus esculentus) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Leonardus B.A. Prakoso ◽  
Christi Mambo ◽  
Mona P. Wowor
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Abelmoschus Esculentus ◽  
Control Group ◽  
Elevated Blood ◽  
Glucose Levels ◽  
Elevated Blood Glucose ◽  
Blood Glucose Levels

Abstract: Okra (Abelmoschus esculentus) is one of many herbal medicine that have been used worldwide to prevent and treat diabetes mellitus. This research aimed to evaluate the effect of garlic bulbs extract administration on elevated blood glucose levels on Wistar rats (R. norvegicus) induced by alloxan. The subject in this research were male Wistar rats with total of 15 samples divided into 5 groups, consisting of one negative control group, one positive control group who were induce by 130 mg/kgBW of alloxan that cause the Rats in conditions of hyperglycemic, and three groups of Rats were given okra extract with dose of 0.375 ml/100 gBW of rat, 0.75 ml/100 gBW of rat , and 1.5 ml/100 gBW of rat. Blood glucose levels was measured on day zero, day one and day two every thirty minutes on 0, 30, 60, 90, 120, and every six hours on 0, 6, 12, 18, and 24. Data from the measurement showed that 0.375 ml/100 gBW, 0.75 ml/100 gBW , and 1.5 ml/100 gBW of okra extract can’t reduce elevated blood glucose levels on Wistar rats.Keywords: Abelmoschus esculentus, okra, blood glucose levels, alloxan Abstrak: Buah Okra (Abelmoschus esculentus) diyakini masyarakat sebagai salah satu tanaman obat yang mampu mencegah dan mengobati penyakit diabetes. Penelitian ini bertujuan untuk menguji apakah pemberian ekstrak buah okra dapat menurunkan kadar glukosa darah tikus Wistar (R. norvegicus) yang diinduksi aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok, terdiri atas satu kelompok kontrol negatif, satu kelompok kontrol positif yang diberi aloksan dengan dosis 130 mg/kgBB yang menyebabkan tikus Wistar dalam keadaan hiperglikemik yang kemudian diinsuksi insulin, dan tiga kelompok Tikus hiperglikemik diberi ekstrak buah okra dengan dosis 0,375 ml/100 gBB tikus, 0,75 ml/100 gBB tikus, dan 1,5 ml/100 gBB tikus. Data diperoleh dari pemeriksaan kadar glukosa darah pada semua kelompok tikus Wistar pada hari ke nol, pertama, dan kedua pada menit ke-0, 30, 60, 90, 120 dan jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak buah okra dengan dosis 0,375 ml/100 gBB tikus, 0,75 ml/100 gBB tikus, dan 1,5 ml/100 gBB tikus tidak mempunyai efek menurunkan kadar glukosa darah tikus Wistar. Kata kunci: abelmoschus esculentus, buah okra, kadar glukosa darah, aloksan

scholarly journals UJI EFEK EKSTRAK UMBI BAWANG PUTIH (Allium sativum L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Bimbi Putri Cahya ◽  
Christi Mambo ◽  
Mona P. Wowor
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Allium Sativum ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Elevated Blood ◽  
Glucose Levels ◽  
Elevated Blood Glucose ◽  
Blood Glucose Levels

Abstract: Garlic (A. sativumL.) is one of many herbal medicine that have been used worldwide to prevent and treat a lot of disease, for example to treat diabetes mellitus. This research aimed to evaluate the effect of garlic bulbs extract administration on elevated blood glucose levels on Wistar rats (R. norvegicus) induced by alloxan. The subjects inthis research were male Wistar rats with total of 18 samples divided into six groups as follows negative control group and five hyperglycemic groups induced by 130 mg/kgBW of alloxan. Garlic bulbs extract was administered with dose of 3 mg/200 gBW of rat, 6 mg/200 gBW of rats, and 12 mg/200 gBW on each groups of hyperglycemic rats, insulin analogue was administered on positive control groups of hyperglycemic rats, and only alloxan was given to the last group. Blood glucose levels was measured on day zero, day one and day two every six hours on 0, 6, 12, 18, and 24. Data from the measurement showed that 6 mg/200 gBW and 12 mg/200 gBW of garlic bulbs extract can reduce elevated blood glucose levels on Wistar rats. Garlic bulbs extract has shown effect on reducing elevated blood glucose levels on Wistar rats induced by alloxan.Keywords: Allium sativum L., Garlic Bulbs, Blood Glucose Levels, AlloxanAbstrak: Bawang putih (A. Sativum L.) diyakini masyarakat sebagai salah satu tanaman obat yang mampu mencegah dan mengobati berbagai penyakit, salah satunya diabetes. Penelitian ini bertujuan untuk menguji apakah pemberian ekstrak umbi bawang putih dapat menurunkan kadar glukosa darah tikus Wistar (R. norvegicus) yang diinduksi aloksan. Subjek penelitian berupa tikus Wistar jantan berjumlah 18 ekor yang dibagi dalam 6 kelompok, terdiri atas satu kelompok kontrol negatif dan lima kelompok tikus Wistar yang diberi aloksan dengan dosis 130 mg/kgBB yang menyebabkan tikus Wistar dalam keadaan hiperglikemik. Tikus hiperglikemik diberi ekstrak umbi bawang putih dengan dosis 3 mg/200 gBB tikus, 6 mg/200 gBB tikus, dan 12 mg/200 gBB tikus, kelompok kontrol positif diberi analog insulin, dan satu kelompok hanya diberi aloksan. Data diperoleh dari pemeriksaan kadar glukosa darah pada semua kelompok tikus Wistar pada hari ke nol, pertama, dan kedua pada jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak umbi bawang putih dengan dosis 6 mg/200 gBB tikus dan 12 mg/200 gBB tikus mempunyai efek menurunkan kadar glukosa darah tikus Wistar.Kata kunci: Allium sativum L., Umbi Bawang Putih, Kadar Glukosa Darah, Aloksan

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) Dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) PADA TIKUS JANTAN PUTIH

2019 ◽  
Vol 2 (1) ◽  
pp. 24-28
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Purpose: To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide. Method: This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg/kgMB, group 3 single roselle extract dose 130 mg/kgMB, group 4 single meniran leaf extract dose 200 mg/kgMB, group 5 combination of meniran leaf extract dose of 100 mg/kgMB and rosella extract dose of 65 mg/kgMB, group 6 combination of meniran leaf extract dose of 200 mg/kgMB and rosella extract dose of 130 mg/kgMB, and group 7 meniran leaf extract combination dose 400 mg/kgMB and rosella extract dose 195 mg/kgMB. Result: The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella gave a significant effect compared to the negative control group did not have a significant effect in reducing blood glucose levels. Conclusion: Single meniran extract and high-dose combination extract are more effective than glibenclamide.

UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) pada TIKUS JANTAN PUTIH SEBAGAI HEWAN PERCOBAAN

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Background : Diabetes mellitus (DM) is a chronic disease caused by the body's inability to produce the hormone insulin or due to ineffective use of insulin production. Diabetes has become a global problem, DM prevalence of 4.6%, estimated in 2000 amounted to 5.6 million. One of the plants that can reduce blood glucose levels is meniran and rosella which are found in Indonesia. Objective : To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide.Method : This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg / kgBB, group 3 single roselle extract dose 130 mg / kgBB, group 4 single meniran leaf extract dose 200 mg / kgBB, group 5 combination of meniran leaf extract dose of 100 mg / kgBB and rosella extract dose of 65 mg / kgBB, group 6 combination of meniran leaf extract dose of 200 mg / kgBB and rosella extract dose of 130 mg / kgBB, and group 7 meniran leaf extract combination dose 400 mg / kgBB and rosella extract dose 195 mg / kgBB. Result : The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella where p <0.05 gave a significant effect compared to the negative control group p> 0.05 did not have a significant effect in reducing blood glucose levels. Conclusion : Single meniran extract and high-dose combination extract are more effective than glibenclamide.

scholarly journals THE EFFECT OF SAUCE OF L. LEUCOCEPHALA SEEDS (LEUCAENA LEUCOCEPHALA [LAM.] DE WIT) ON LOWERING BLOOD GLUCOSE IN HYPERGLYCEMIC MICE

2018 ◽  
Vol 11 (13) ◽  
pp. 70
Author(s):  
Sarah Zaidan ◽  
Ria Debby Bp ◽  
Syamsudin Abdillah
Keyword(s):  
Blood Glucose ◽  
Leucaena Leucocephala ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Group I ◽  
Group Ii

 Objective: The research to investigate that the source of Leucaena leucocephala can lower blood glucose levels in hyperglycemic mice.Methods: In this study, 42 mice were divided into 7 groups each consisted of 6 mice: Normal Group I, Group II (metformin positive control), Group III (negative control), Group IV (sauce of L. leucocephala at a dose of 0.1 ml), Group V (sauce of L. leucocephala at a dose of 0.2 ml), Group VI (sauce of L. leucocephala with doses of 0.4 ml), and Group VII (sauce of L. leucocephala at a dose of 0.8 ml). The dosage of Group II, IV, V, VI, and VII was orally administered. Blood glucose levels in mice were observed during normal conditions and after administration of 200 mg/kg bw alloxan intraperitoneally (alloxan-induced mice). On day 0, 3, 7, and 14, blood was taken from the tail and glucose levels were measured with a glucometer.Results: Data were analyzed by one-way analysis of variance followed by LSD test. Based on the results, the sauce at a dose of 0.8 ml was able to lower the blood glucose levels up to normal blood glucose levels.Conclusion: There was not significantly different between the groups given sauce of L. leucocephala at a dose of 0.8 ml with the group given metformin (positive control).

scholarly journals THE EFFECT OF AMINO ACID COMPOUNDS IN FERMENTED SOYBEANS AGAINST FIBROBLAST GROWTH FACTOR IN MICE PANCREATIC β-CELLS FIGURES

2018 ◽  
Vol 11 (9) ◽  
pp. 157
Author(s):  
Surya Dharma ◽  
Dedy Almasdy ◽  
Dwisari Dillasamola ◽  
Roslinda Rasyid ◽  
Dianty Dwi Wahyuni ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Fibroblast Growth ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Experimental Animals ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective: The aim of this study is to determine the effect of the amino acid compound in fermented soybeans against fibroblast growth factor (FGF) in mice’s pancreatic β-cell figures. This study was also done to know the effect of given combination of fertilized egg whites powder with tempe (Indonesian conventional food and the fermentation product of soybeans fermentation) in a dose of 7250 mg/kg.Methods: All of the experimental animals pancreases were designed to be damaged by given alloxan in a dose of 150 mg/kg, except for the negative control group without anything given. The experimental animals were divided into 7 groups which consist of the negative control group, positive control group, and the rests 5 group of FGF test preparation in dosages of 100; 140; 200; 300; and 425 mg/kg combined with fermented soybeans in a dose 7250 mg/kg. This experiment was conducted for 21 days, observed at 7th, 14th, and 21st day. The data analysis used is a statistical test of one-way analysis of variance (ANOVA) and two-way ANOVA.Results: The result showed a significant decrease in blood glucose levels (p<0.05) in mice for all treatments when compared with positive controls. From the result of the histopathologic examination, pancreatic β-cells improve utterly close to the control condition (-). In the qualitative immunohistochemical examination, there was a difference in the stained pancreatic β-cells marked by yellow density. Meanwhile, the quantitative observation did not show any improvement against normal condition control (-) (p<0.05).Conclusion: The combination of egg whites and fermented soybean significantly decreased the blood glucose levels, and the occurrence of the Langerhans island cells was nearly normal.

scholarly journals Uji efek ekstrak daun papaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Cynthia C.C. Senduk ◽  
Henoch Awaloei ◽  
Edward Nangoy
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Control Group ◽  
Elevated Blood ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Abstract: Indonesia is a tropical country with more than 9,600 kinds of medicinal plants; one of them is papaya. Extract of papaya leaf (Carica papaya) is presumed to have hypoglycemia effect because it contains flavonoid, alkaloid, saponin, and tannin. This study aimed to evaluate the effect of papaya leaf extract on elevated blood glucose levels on Wistar rats induced with alloxan. This was an experimental study with 18 male Wistar rats as subjects, divided into 6 groups (3 rats in each group). Group 1, the negative control group, was given aquadest only. Group 2, the positive control group, was treated with alloxan 120 mg/kg body weight (BW) followed by novomix 0.2 iu/200 g BW. Group 3 and 4 were treated with alloxan 120 mg/kg BW followed by papaya leaf extract dosing 250 mg and 500 mg/kg BW respectively. Group 5 and 6 were treated with papaya leaf extract dosing 250 mg and 500 mg/kg BW without alloxan induction. Blood glucose levels were measured on day 1, day 2, and day 3 every six hours at 0, 6, 12, 18, and 24 hours. The results showed that 250 mg/kg BW and 500 mg/kg BW of papaya leaf extract could reduce the elevated blood glucose on Wistar rats for 12 hours after treatment. Conclusion: The extract of papaya leaves could reduce blood sugar levels in hyperglicemic Wistar rats induced by alloxan. Keywords: papaya leaves (carica papaya L.), blood sugar levels, alloxan. Abstrak: Indonesia merupakan salah satu negara beriklim tropis yang memiliki banyak jenis tumbuhan. Sekitar 9.600 spesies tumbuhan merupakan tumbuhan yang berkhasiat obat, salah satunya ialah pepaya. Ekstrak daun pepaya (Carica papaya L.) diduga mempunyai efek hipoglikemia karena mengandung flavonoid, alkaloid, saponin, dan tannin. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak daun pepaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan. Jenis penelitian ini eksperimental. Subyek penelitian yang digunakan ialah 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok (setiap kelompok terdiri dari 3 tikus). Kelompok 1 (K1) merupakan kelompok kontrol negatif hanya diberikan aquades; kelompok 2 (K2) merupakan kelompok kontrol positif diberikan aloksan dan novomix 0,2 iu/200 g BB; kelompok 3 (K3) dan kelompok 4 (K4) merupakan kelompok perlakuan diberikan aloksan kemudian ekstrak daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB; kelompok 5 (K5) dan kelompok 6 (K6) merupakan kelompok perlakuan diberikan daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB tanpa induksi aloksan. Data diperoleh dari pemeriksaan kadar gula darah dari semua kelompok tikus Wistar pada hari ke-1, ke-2, dan ke-3 pada jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pepaya dengan dosis 250 mg dan 500 mg/kg BB tikus berefek menurunkan kadar gula darah tikus wistar selama 12 jam pasca pemberian ekstrak daun pepaya. Simpulan: Ekstrak daun pepaya berpotensi memiliki efek dalam menurunkan kadar gula darah pada tikus Wistar.Kata kunci: daun pepaya (carica papaya L.), kadar gula darah, aloksan

scholarly journals PENGARUH PEMBERIAN EKSTRAK DAUN SIRSAK (Annona Muricata L.) TERHADAP KADAR GULA DARAH TIKUS WISTAR (Rattus norvegicus) YANG DIINDUKSI ALLOXAN

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Febbyola S. Moniaga
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Wistar Rat ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Annona Muricata ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Abstract: People sure that Soursop leaves (A.muricata L.) has a lot of advantage for health, one example is to reduced blood glucose levels. The objectives of this research were to study the effectiveness of administration of soursop leaves extract (A.muricata L.) on blood glucose levels of wistar rat (R.norvegicus) induced by alloxan. The subjects in these research were male wistar rat with total of 18 samples divided into 6 groups as follows negative control group and 5 hyperglycemic groups induced by alloxan with dose 110 mg/kg bodyweight of rat. Hyperglycemic rats administered by soursop leaves extract dosing 1000 mg, 2000 mg, and 5000 mg/kg bodyweight of rat, positive control groups administered by novomix flexpen, and the last one only administered by alloxan. Data taken from measurement of blood sugar on first day, second day, and third day on minutes 0,30,60,90, and 120. The result showed reduction of alloxan-induced wistar rat’s blood sugar levels on administration of soursop leaves extract. Keyword: Annona Muricata L, Soursop leaves, Blood sugar levels, Alloxan.       Abstrak: Daun sirsak (A.muricata L.) diyakini masyarakat memiliki banyak manfaat bagi kesehatan salah satunya menurunkan kadar gula darah. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak daun sirsak (A.muricata L.) terhadap kadar gula darah tikus Wistar (R.norvegicus) yang diinduksi alloxan. Subjek penelitian berupa tikus Wistar jantan berjumlah 18 ekor yang dibagi dalam 6 kelompok, kelompok kontrol negatif dan 5 kelompok tikus Wistar hiperglikemik akibat pemberian alloxan dengan dosis 110 mg/kg berat badan tikus. Tikus hiperglikemik diberi ekstrak daun sirsak dosis 1000, 2000, dan 5000 mg/kg BB tikus,  kelompok kontrol positif yang diberi novomix flexpen, dan 1 kelompok hanya diberi alloxan. Data diperoleh dari pemeriksaan kadar gula darah pada semua kelompok tikus Wistar pada hari pertama, hari kedua , hari ketiga pada menit ke-0 ,30, 60, 90, dan menit ke-120. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirsak mempunyai efek terhadap penurunan kadar gula darah tikus Wistar yang telah diinduksi alloxan. Kata kunci: Annona Muricata L., Daun Sirsak, Kadar Gula Darah, Alloxan.

scholarly journals UJI EFEK PEMBERIAN EKSTRAK BIJI PETAI CINA (Leucaena leucochepala L) TERHADAP KADAR GULA DARAH TIKUS WISTAR (Rattus norvegicus) YANG DIINDUKSI DENGAN ALOKSAN

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Mahesti Utami ◽  
Mona P. Wowor ◽  
Christi Mambo
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose ◽  
Tree Seeds ◽  
Tree Seed

Abstract: Public believes that Lead Tree seeds (Leucaena leucochepala L) as a kind of medicinal plant that have capability of treating a lot of diseases, among them is diabetes. This study aimed to test whether the Lead tree seeds extract can reduce blood glucose levels of Wistar rats (R. norvegicus) which has induced alloxan. The subjects in these research were male wistar rat with total of 18 samples divided into six groups consisting of a negative control group and five groups induced by alloxan with dose 130 mg/kgBW of rat, which can cause wistar rats in hyperglycemic state. Hyperglycemic rats were given lead tree seed extract at a dose of 0,14 mg/200 gBW, 0,3 mg/200 gBW, and 0,6 mg/200 gBW, positive control group was given insulin analogue, and one group was given alloxan. Data obtained from the examination of blood glucose levels in all groups of Wistar rats on day zero, day one and day two every six hours on 0, 6, 12, 18, and 24. The results showed that giving lead tree seed extract does not have the effect to reduce blood glucose levels Wistar rats.Keywords: leucaena leucochepala L., lead tree seed, bood glucose level, alloxanAbstrak: Biji petai cina (Leucaena leucochepala L)diyakini masyarakat sebagai salah satu tanaman obat yang mampu mengobati berbagai penyakit, salah satunya diabetes. Penelitian ini bertujuan untuk menguji apakah pemberian ekstrak biji petai cina dapat menurunkan kadar glukosa darah tikus Wistar (R. norvegicus) yang diinduksi aloksan. Subjek penelitian berupa tikus Wistar jantan berjumlah 18 ekor yang dibagi dalam 6 kelompok, terdiri atas satu kelompok kontrol negatif dan lima kelompok tikus Wistar yang diberi aloksan dengan dosis 130 mg/kgBB yang menyebabkan tikus Wistar dalam keadaan hiperglikemik. Tikus hiperglikemik diberi ekstrak biji petai cina dengan dosis 0,14 mg/200 gBB tikus, 0,3 mg/200 gBB tikus, dan 0,6 mg/200 gBB tikus, kelompok kontrol positif diberi analog insulin, dan satu kelompok hanya diberi aloksan. Data diperoleh dari pemeriksaan kadar glukosa darah pada semua kelompok tikus Wistar pada hari ke nol, pertama, dan kedua pada jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak biji petai tidak mempunyai efek menurunkan kadar glukosa darah tikus Wistar.Kata kunci: leucaena leucochepala L., biji petai cina, kadar gula darah, aloksan

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