scholarly journals Errors in the Vickers diagonal length measurement caused by different designs of microscopes

ACTA IMEKO ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 261
Author(s):  
S. Takagi ◽  
Y. Tanaka ◽  
Y. Seino
Keyword(s):  
Numerical Aperture ◽  
Image Resolution ◽  
Point Of View ◽  
Length Measurement ◽  
Resolving Power ◽  
Measuring System ◽  
Objective Lens ◽  
Depth Of Focus ◽  
The Relationship ◽  
Diagonal Length

The errors in the Vickers diagonal length measurement are discussed from the point of view of the design of microscope. Effects of the numerical aperture (NA) of an objective lens are evaluated through the experiment. With smaller NA, the resolving power of the microscope gets worse and the depth of focus gets deeper as predicted Rayleigh’s criterion. The relationship between the repeatability of diagonal length and the objective NA follows the theory. In addition, the variation of NA relates the measured diagonal length. Those effects are much significant than the image resolution or the minimum count of a measuring system.

Development of High Resolution Electron Microscope, JEM-100B

1968 ◽  
Vol 26 ◽  
pp. 298-299
Author(s):  
M. Watanabe ◽  
T. Yanaka ◽  
M. Yamamoto ◽  
S. Suzuki ◽  
Y. Nagahama ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Spherical Aberration ◽  
Point Of View ◽  
Resolving Power ◽  
Objective Lens ◽  
Stage Of Development ◽  
Resolution Electron ◽  
Microscopic Studies ◽  
High Resolution Electron Microscope ◽  
The Stability

The JEM-100B electron microscope has been developed with a view to the possibility of obtaining an ultimate resolving power at a beam accelerating voltage of 100kev. Prom the very nature of the instrument, the stability is fully ensured from a mechanical and electrical point of view. In the electron optical system, since highly excited lenses are utilized, image formation under the lowest aberration condition are ensured. The image forming system is of the 4-stage type which greatly expands the range of application not only in microscopic studies but also in the study of electron diffraction. Furthermore, with a view to simplifying instrument operation, various problems have been solved this enabling the instrument to be fully automated. The JEM-100B exhibits the following features.Objective Lens: At the present stage of development, fo=1.6mm. However, so as to be able to incorporate a lens (T. Yanaka et al, 1967) possessing an extremely small spherical aberration coefficient in the future, lens excitation has been made sufficiently high (8kA) and the movable aperture control knob and the specimen device have been designed accordingly.

High-Resolution Tolerance Against Noise Imaging Technique Based on Active Shift of Optical Axis

2011 ◽  
Vol 5 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Shin Usuki ◽  
◽  
Kenjiro T. Miura ◽  
Keyword(s):  
Optical Imaging ◽  
Optical Axis ◽  
Glass Plate ◽  
Computational Cost ◽  
Numerical Aperture ◽  
Image Resolution ◽  
Objective Lens ◽  
Optical Parameters ◽  
Modulation Transfer ◽  
Pixel Resolution

Optical imaging resolution is determined by both optical parameters such as light wavelength and the objective lens Numerical Aperture (NA) and by spatial sampling such as Charge-Coupled Device (CCD) camera pixel size. Focused on improving optical imaging pixel resolution, we reviewed multiframe Super-Resolution (SR) implemented in previous measurement to improve image resolution, but found its computational cost to high and calculation too long. Pixeldisplacement estimation also adversely affects resulting image quality and is difficult to apply practically due to SR registration calculation sensitivity to noise. The resolution improvement we have proposed uses active subpixel shifting of the optical axis based on actively controlling spatial image displacement using a glass-plate-parallel substrate and a galvano scanner. Multiframe SR registration is used but without pixel-displacement estimation. Theoretically, our proposal may improve image resolution stably at higher speed but to clarify this, we developed optics and rebuilt the multiframe SR.We then computationally analyzed improved resolution and noise tolerance using a Modulation Transfer Function (MTF). Optical and aliasing noise have been suppressed and image resolution improved in high measurement, making higherresolution imaging faster and cheaper thanks to our proposal.

Practical High Resolution Microscopy

1968 ◽  
Vol 26 ◽  
pp. 302-303
Author(s):  
P. A. Marsh ◽  
T. Mullens ◽  
D. Price
Keyword(s):  
High Resolution ◽  
Magnetic Fields ◽  
Low Frequency ◽  
Resolving Power ◽  
Careful Attention ◽  
Electron Microscopes ◽  
The Relationship ◽  
High Resolution Microscopy ◽  
New Facilities

It is possible to exceed the guaranteed resolution on most electron microscopes by careful attention to microscope parameters essential for high resolution work. While our experience is related to a Philips EM-200, we hope that some of these comments will apply to all electron microscopes.The first considerations are vibration and magnetic fields. These are usually measured at the pre-installation survey and must be within specifications. It has been our experience, however, that these factors can be greatly influenced by the new facilities and therefore must be rechecked after the installation is completed. The relationship between the resolving power of an EM-200 and the maximum tolerable low frequency interference fields in milli-Oerstedt is 10 Å - 1.9, 8 Å - 1.4, 6 Å - 0.8.

Magnetic Domain Observation of an Amorphous Fe-Si-B Ribbon by Means of a High Voltage Scanning Electron Microscope

1981 ◽  
Vol 39 ◽  
pp. 320-321
Author(s):  
K. Tsuno ◽  
Y. Harada ◽  
T. Sato
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
High Voltage ◽  
Amorphous Ribbon ◽  
Image Resolution ◽  
Objective Lens ◽  
Magnetic Domains ◽  
Scattered Electron ◽  
Voltage Scanning ◽  
Scanning Electron

Magnetic domains of ferromagnetic amorphous ribbon have been observed using Bitter powder method. However, the domains of amorphous ribbon are very complicated and the surface of ribbon is not flat, so that clear domain image has not been obtained. It has been desired to observe more clear image in order to analyze the domain structure of this zero magnetocrystalline anisotropy material. So, we tried to observe magnetic domains by means of a back-scattered electron mode of high voltage scanning electron microscope (HVSEM).HVSEM method has several advantages compared with the ordinary methods for observing domains: (1) high contrast (0.9, 1.5 and 5% at 50, 100 and 200 kV) (2) high penetration depth of electrons (0.2, 1.5 and 8 μm at 50, 100 and 200 kV). However, image resolution of previous HVSEM was quite low (maximum magnification was less than 100x), because the objective lens cannot be excited for avoiding the application of magnetic field on the specimen.

Scanning tunneling microscopy (STM) of DNA and RNA

1989 ◽  
Vol 47 ◽  
pp. 34-35
Author(s):  
Patricia G. Arscott ◽  
Gil Lee ◽  
Victor A. Bloomfield ◽  
D. Fennell Evans
Keyword(s):  
Molecular Structures ◽  
Inorganic Materials ◽  
Resolving Power ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Form And Function ◽  
Dna And Rna ◽  
Calf Thymus ◽  
And Function ◽  
The Relationship

STM is one of the most promising techniques available for visualizing the fine details of biomolecular structure. It has been used to map the surface topography of inorganic materials in atomic dimensions, and thus has the resolving power not only to determine the conformation of small molecules but to distinguish site-specific features within a molecule. That level of detail is of critical importance in understanding the relationship between form and function in biological systems. The size, shape, and accessibility of molecular structures can be determined much more accurately by STM than by electron microscopy since no staining, shadowing or labeling with heavy metals is required, and there is no exposure to damaging radiation by electrons. Crystallography and most other physical techniques do not give information about individual molecules.We have obtained striking images of DNA and RNA, using calf thymus DNA and two synthetic polynucleotides, poly(dG-me5dC)·poly(dG-me5dC) and poly(rA)·poly(rU).

Towards 1-Ångstrom-resolution STEM

1993 ◽  
Vol 51 ◽  
pp. 996-997
Author(s):  
H.S. von Harrach ◽  
D.E. Jesson ◽  
S.J. Pennycook
Keyword(s):  
High Resolution ◽  
Field Emission ◽  
Phase Contrast ◽  
Spherical Aberration ◽  
A Priori ◽  
Dark Field ◽  
Image Resolution ◽  
Objective Lens ◽  
Annular Dark Field ◽  
First Results

Phase contrast TEM has been the leading technique for high resolution imaging of materials for many years, whilst STEM has been the principal method for high-resolution microanalysis. However, it was demonstrated many years ago that low angle dark-field STEM imaging is a priori capable of almost 50% higher point resolution than coherent bright-field imaging (i.e. phase contrast TEM or STEM). This advantage was not exploited until Pennycook developed the high-angle annular dark-field (ADF) technique which can provide an incoherent image showing both high image resolution and atomic number contrast.This paper describes the design and first results of a 300kV field-emission STEM (VG Microscopes HB603U) which has improved ADF STEM image resolution towards the 1 angstrom target. The instrument uses a cold field-emission gun, generating a 300 kV beam of up to 1 μA from an 11-stage accelerator. The beam is focussed on to the specimen by two condensers and a condenser-objective lens with a spherical aberration coefficient of 1.0 mm.

Microspectroscopy Using a Solid Immersion Lens

2001 ◽  
Vol 7 (S2) ◽  
pp. 148-149
Author(s):  
C.D. Poweleit ◽  
J Menéndez
Keyword(s):  
Spatial Resolution ◽  
Focal Plane ◽  
Numerical Aperture ◽  
Normal Incidence ◽  
Spot Size ◽  
Index Of Refraction ◽  
Objective Lens ◽  
Improvement Factor ◽  
Oil Immersion ◽  
Long Time

Oil immersion lenses have been used in optical microscopy for a long time. The light’s wavelength is decreased by the oil’s index of refraction n and this reduces the minimum spot size. Additionally, the oil medium allows a larger collection angle, thereby increasing the numerical aperture. The SIL is based on the same principle, but offers more flexibility because the higher index material is solid. in particular, SILs can be deployed in cryogenic environments. Using a hemispherical glass the spatial resolution is improved by a factor n with respect to the resolution obtained with the microscope’s objective lens alone. The improvement factor is equal to n2 for truncated spheres.As shown in Fig. 1, the hemisphere SIL is in contact with the sample and does not affect the position of the focal plane. The focused rays from the objective strike the lens at normal incidence, so that no refraction takes place.

Hubungan Kemendagri dan Kemendes dalam Tata Desa dan Administrasi Desa

2020 ◽  
Vol 3 (2) ◽  
pp. 81-97
Author(s):  
Sarip Sarip ◽  
Nur Rahman ◽  
Rohadi Rohadi
Keyword(s):  
Constitutional Law ◽  
Administrative Law ◽  
Point Of View ◽  
Legal Research ◽  
The Government ◽  
The Village ◽  
The Relationship

This article aims to explore the relationship between the Ministry of Home Affairs (Kemendagri) and the Ministry of Villages (Kemendes) from theconstitutional law and state administrative law point of view.The second concerns of this research is the disharmony and problem between the two ministries.From the constitutional law point of view, it turns out that what the Ministry of Home Affairs is doing, is closer to the object of its discussion. The method used in this research is normative legal research bycomparingthe constitutional law and state administrative law to obtain clarity regarding the Ministry of Home Affairs and Ministry of Village. The result shows that the Ministry of Village approached the science of state administrative law, namely to revive or give spirits to the village. Disharmonization began to exist since the inception of the Ministry of Village. The root of disharmony itself was the improper application of constitutional foundations in the formation of the Village Law. It would be better if the government reassess the constitutional foundation for the village.

FORGOTTEN UNITY OF BODY AND SOUL AND THE NEED FOR A NEW PHILOSOPHY OF SPORT

2018 ◽  
Vol 28 (7) ◽  
pp. 2523-2529
Author(s):  
Slobodan Marković ◽  
Zoran Momčilović ◽  
Vladimir Momčilović
Keyword(s):  
Ancient Greece ◽  
Point Of View ◽  
Greek Philosophy ◽  
Ancient Greek ◽  
Constructive Dialogue ◽  
Ancient Greek Philosophy ◽  
Philosophy Of Sport ◽  
Body And Soul ◽  
The Relationship ◽  
Present Text

This text is an attempt to see sport in different ways in the light of ancient philosophical themes. Philosophy of sports gets less attention than other areas of the discipline that examine the other major components of contemporary society: philosophy of religion, political philosophy, aesthetics, and philosophy of science. Talking about sports is often cheap, but it does not have to be that way. One of the reasons for this is insufficiently paid attention to the relation between sport and philosophy in Greek. That is it's important to talk about sports, just as important as we are talking about religion, politics, art and science. The argument of the present text is that we can try to get a handle philosophically on sports by examining it in light of several key idea from ancient Greek philosophy. The ancient Greeks, tended to be hylomorphists who gloried in both physical and mental achievement. Тhe key concepts from Greek philosophy that will provide the support to the present text are the following: arete, sophrosyne, dynamis and kalokagathia. These ideals never were parts of a realized utopia in the ancient world, but rather provided a horizon of meaning. We will claim that these ideals still provide worthy standards that can facilitate in us a better understanding of what sports is and what it could be. How can a constructive dialogue be developed which would discuss differences in understanding of sport in Ancient Greece and today? In this paper, the authors will try to answer this question from a historical and philosophical point of view. The paper is divided into three sections. The first section of the paper presents two principally different forms or models of focus in sport competitions – focus on physical excellence or focus on game. The dialectic discourse regarding these two approaches to physical activity is even more interesting due to the fact that these two models take precedence over one another depending on context. In the second section of the paper, the focus shifts to theendemic phenomenon of the Ancient Greek Olympic Games, where the topic is discussed from the perspective of philosophy with frequent historical reflections on the necessary specifics, which observeman as a physical-psychological-social-spiritual being. In the third section of this paper, the authors choose to use the thoughts and sayings of the great philosopher Plato to indicate how much this philosopher wasactually interested in the relationship between soul and body, mostly through physical exercise and sport, because it seems that philosophers who came after him have not seriously dealt with this topic in Plato’s way, although they could.

О вопросах фольклоризма в письменной литературе

2019 ◽  
pp. 31-33
Author(s):  
Суусар Искендерова
Keyword(s):  
Modern Science ◽  
Folk Songs ◽  
Point Of View ◽  
The Past ◽  
Scientific Term ◽  
Literature Culture ◽  
Definition Of ◽  
The Relationship

Аннотация: Исследование проблемы фольклоризма является наиболее актуальной в современной науке о фольклоре. На разных этапах развития художественной литературы для формирования индивидуального творчества писателя особенно значимым становятся фольклорные жанры, сюжетные мотивы и художественные средства. В статье рассматривается связь письменной литературы и фольклора, особенно точка зрения проблеме фольклоризма в прошлом и их анализ. Термин «фольклоризм» начал использоваться советскими исследователями учеными как научный термин еще в 1930-х гг. Термин «фольклоризм» используется в различных сферах культуры, а в этой статье мы будем рассматривать в литературе. Несмотря на то, что на протяжении многих лет этот вопрос изучается литературоведами, фольклористами, все -таки нет единого теоретического определения понятия. Ключевые слова: фольклор, фольклоризм, литература, культура, письменная литература, художественная литература, оседлый народ, пословицы и поговорки, фольклорные песни. Аннотация: Көркөм адабияттын өнүгүүсүнүн ар кайсы баскычтарында сүрөткердин жеке чыгармачылыгынын калыптанышы үчүн фольклордук жанрлар, сюжеттер, мотивдер жана көркөм каражаттар айрыкча мааниге ээ. Макалада жазма адабият менен фольклордук карым-катышы, айрыкча фольклоризм маселеси жөнүндө мурдагы көз караштарга кайрылып, аларга талдоо жүргүзүү менен бирге автор өз байкоолорунда келтирет. “Фольклоризм” деген илимий термин 1930-жылы баштап колдонула баштаган. “Фольклоризм” термини маданияттын түрдүү сфераларында кеңири колдо- нулат, бул жерде адабияттагы колдонулушун каралат. Макалада адабий материал менен фольклордук байланышын терең түшүнүү үчүн адабий фольклоризм маселесинин талаштуу жактары каралат. Түйүндүү сөздөр: фольклор, фольклоризм, адабият, маданият, жазма адабият, көркөм адабият, көчмөн калк, макал-лакап, фольклордук ырлар. Annotation: The study of the problem of folklore is the most relevant in the modern science of folklore. At various stages in the development of fiction, folklore genres, plot motifs, and artistic means become especially significant for the formation of the writer's individual creativity. The article examines the relationship between written literature and folklore, especially the point of view of the problem of folklorism in the past and their analysis. The term "folklorism" began to be used by Soviet scholars as a scientific term back in the 1930s. The term "folklorism" is used in various fields of culture, and in this article we will consider in the literature. Despite the fact that for many years this issue has been studied by literary scholars, folklorists, all the same there is no single theoretical definition of the concept. Keywords: folklore, folklorism, literature, culture, written literature, fiction, settled people, proverbs and sayings, folk songs.

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