scholarly journals The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

2018 ◽  
Vol 42 (2) ◽  
Author(s):  
Muhammad Rizal ◽  
Muhammad Riyadhi ◽  
Abrani Sulaiman
Keyword(s):  
Plasma Membrane ◽  
Seminal Plasma ◽  
Egg Yolk ◽  
Boer Goat ◽  
The Third ◽  
Palm Juice ◽  
Boer Goats ◽  
Fresh Semen ◽  
Artificial Vagina

The objective of this study was to examine the effect of seminal plasma on viability of Boer goat spermatozoa and effectiveness of sugar palm juice as an alternative extender during preservation at 5ºC. Semen of two Boer goats were collected using an artificial vagina. Fresh semen were evaluated and divided in equal volume into four tubes. Semen in the first and second tubes diluted with 80% sugar palm juice + 20% egg yolk (P1) and Andromed (P2), respectively. Semen in the third and fourth tubes were centrifuged with 3,000 RPM for 20 minutes, and the supernatant removed. diluted with 80% sugar palm juice + 20% egg yolk (P3) and Andromed (P4), respectively. Diluted-semen were preserved in refrigerator at 5oC, and quality of the spermatozoa including motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated every day for four days. Results of this study showed that at day-2 preservation, mean percentages of MS, LS, and IPM for P2 (72, 83.4, and 83.4%), P3 (72, 82.6, and 82.2%), and P4 (72, 83, 83.8%) were significantly (P<0.05) higher than P1 (3, 24.8, and 25.2%). At day-3 preservation, mean percentages of MS, LS, and IPM for P2 (57, 65.6, and 69.6%) was significantly (P<0.05) higher than P3 (21, 34.8, and 31.8%), P4 (22, 33.6, and 31.2%), and P1 (0, 0, and 0%). In conclusion, semen of Boer goat to be preserved with extender containing egg yolk should be removed seminal plasma. Sugar palm juice containing egg yolk could be used as an extender for Boer goat semen, but should be applied in the AI program immediately after the semen is diluted.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE)

2019 ◽  
Vol 20 (1) ◽  
pp. 20 ◽  
Author(s):  
ALoysius Marawali ◽  
Muhammad S. Abdullah ◽  
Jalaludin Jalaludin
Keyword(s):  
Statistical Analysis ◽  
Cold Shock ◽  
Egg Yolk ◽  
Coconut Water ◽  
The Third ◽  
Bali Cattle ◽  
Artificial Vagina

The aim of this research was to know the effectiveness of guava filtrate supplementation in coconut water- egg yolk dilution on quality of liquid semen stored at 5oC of Bali cattle. Semen collected from a five year old Bali cattle using artificial vagina. Semen of good quality were kept in six tubes based on treatment then stored at 5oC. Treatments of the research were P0 : coconut water 80% + egg yolk 20% without guava filtrate; P1 : coconut water 80% + egg yolk 20% + 0.8% guava filtrate; P2 : coconut water 80% + egg yolk 20% + 0.9% guava filtrate; P3 : coconut water 80% + egg yolk 20% + 1.0 % guava filtrate; P4 : coconut water 80% + egg yolk 20% + 1.1 % guava filtrate and P5 : coconut water 80% + egg yolk 20% + 1.2 % guava filtrate. Each treatment was replicated 8 times making 48 experimental units. Results of the study showed that percentage mean of motility, viability, MPU, and TAU of spermatozoa after three days storage for P0 were : 42.20%, 41.85%, 39.08% and 40.90%; P1 : 50.40%, 53.89%, 52.99% and 54.67%; P2 : 54.67%, 56.97%, 54.51% and 54.36%; P3 : 17.00%, 29.96%, 29.64% and 29.64%; P4 : 23.38%, 24.64%, 21.06% and 24.45%Jurnal Veteriner Maret 2019 Vol. 20 No. 1 : 20 -29 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.20 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/201621PENDAHULUAN Salah satu solusi yang dapat digunakan untuk pengembangan program Inseminasi buatan (IB) secara cepat dan mudah pada sapi bali adalah penggunaan semen cair. Penggunaan semen cair dapat meningkatkan kinerja IB pada sapi bali di Nusa Tenggara Timur (NTT). Keunggulan lain semen cair dapat diproduksi menggunakan bahan pengencer herbal berbasis bahan lokal dan peralatan yang sederhana serta mudah diperoleh dan tidak tergantung dengan persediaan nitrogen cair. Hasil akhir dari metabolisme spermatozoa adalah terbentuknya radikal bebas berupa derivat oksigen di antaranya adalah single1 oksigen (1O2), tripel1 oksigen (3O2), superokside anion (O2-), hidroksil radikal (OH) dan nitrit oxide (NO-) yang semuanya disebut radical oksigen species (ROS). Single1 oksigen dapat merusak ikatan rangkap pada asam lemak sehingga dapat menyebabkan kerusakan Deoxyribo Nuclead Acid (DNA) dan protein. Single1 oksigen bila bereaksi dengan asam amino histidin akan membentuk enzim yang dapat menyebabkan denaturasi protein. Kerusakan spermatozoa pada penyimpanan suhu 5%C akibat radikal bebas dan cold shock inilah merupakan penyebab utama disfungsi semen (Sharma et al., 2000). Oksidasi fosforilasi yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang tinggi dalam sel dapat mengoksidasi lipid, protein dan DNA. Lipid membran plasma semen memiliki fosfolipid dengan kadar yang tinggi menyebabkan semen rentan terhadap radikal bebas (Sanoeka dan Kurpisz, 2004). Antioksidan bertindak mengikat asam lemak tak jenuh dan mencegah terjadinyareaksi berantai. Pada proses penyimpanan semen akan terjadi kerusakan membran plasma spermatozoa akibat terbentuknya perioksidasi lipid. Antioksidan-pemutus rantai seperti yang terkandung dalam jambu biji dapat menghambat perioksidasi lipid dalam membran melalui radical peroxyl (RO) dan alkoxyl (ROO) pengurai. Pengunaan jambu biji yang difilter dalam pengencer air kelapa kuning telur dapat menjaga kualitas spermatozoa (motilitas, keutuhan akrosom, viabilitas dan morfologi spermatozoa) semen cair sapi bali selama penyimpanan pada suhu 5%C. Dosis jambu biji yang difilter yang terbaik dalam pengencer air kelapa kuning telur, akan terbaik pula dalam mempertahankan kualitas spermatozoa sampai tujuan IB. Adapun tujuan penelitian ini adalah menguji berbagai level pemberian filtrat jambu biji (FJB) dalam pengencer air kelapa kuning telur terhadap motilitas, viabilitas, membran plasma utuh (MPU) dan tudung akrosom utuh (TAU) spermatozoa sapi bali yang disimpan pada suhu 5%C.METODE PENELITIAN Penelitian ini telah dilakukan di Laboratorium Reproduksi milik Yayasan Wiliams dan Laura yang berlokasi di Tilong, Desa Oelnasi, Kec. Kupang Tengah, Kab. Kupang, Nusa Tenggara Timur, dan berlangsung selama delapan bulan. Materi yang digunakan dalam penelitian ini adalah semen sapi bali yang ditampung dari satu ekor sapi bali jantan berumur lima tahun milik Yayasan Williams dan Laura yang telah dilatih, memiliki performans yang baik, dan organ reproduksi normal. Pakan yang diberikan adalah hijauan berupa rumput dan legum dan pemberian konsentrat secukupnya (dedak padi dan jagung giling).and P5 : 9%, 21.25%, 17.56% and 19.30%. Result of statistical analysis showed that there were a significant effect (P<0.05) between treatment on motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage. It can be concluded that the supplementation of guava filtrate 0.9% in dilution of coconut water 80% - egg yolk 20% had been able to maintain motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage at 5oC.

scholarly journals Relationship of extender and packaging system an the length of preservation and the quality of chilled semen of Boer goat

2016 ◽  
Vol 21 (1) ◽  
pp. 49
Author(s):  
Arie Febretrisiana ◽  
. Anwar ◽  
Simon Sinulingga
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Packaging System ◽  
Boer Goat ◽  
Sperm Membrane ◽  
Relationship Of ◽  
Artificial Vagina

<p class="abstrak2">The aim of this research was to compare the effectiveness of different extender (either Triladyl or Tris Egg Yolk extender) and different packaging method (pool and straw) of chilled semen an the length of preservation and the quality of chilled semen of Boer goat. Semen was collected using an artificial vagina from 3 two years old Boer bucks with body weight of 50-55 kg. It was evaluated under a microscope, then each was diluted either in Tris egg yolk extender (TEY) or Triladyl. Those diluted sperms were then packed either in pool or straw and preserved at 5⁰C refrigerator. Sperm motility, viability and membrane integrity of each group were evaluated every 24 h for up to 5 days. Results showed that sperm motility in Triladyl of  pool packaging system up to 3 days was higher than straw packaging system or TEY in pool or straw packaging system which were 45.8%, 26.1%, 32.1% and 9.1%, respectively (P&lt;0.05). Percentage of sperm membrane integrity showed the same pattern to Triladyl both in pool and straw packaging system which was higher than TEY group (75.2% and 77,2%; P&lt;0.05). Sperm viability in Triladyl both in pool or straw packaging system decreased (P&lt;0.05) after 3 days of preservation (77.1% and 76.2%) but TEY significanly decreased after 4 days of preservation either in pool or straw packaging system (73.2% and 58.0%; P&lt;0.05). It was concluded that sperm quality decreased with increasing of the length of preservation while Triladyl extender in pool packaging system showed the best quality.</p><strong>Key Words: </strong>Chilled Semen, Boer, Triladyl, Tris Egg Yolk, Straw

scholarly journals KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING

2017 ◽  
Vol 16 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Herdis Suharman
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Ovis Aries ◽  
Initial Evaluation ◽  
Freezing Process ◽  
Frozen Semen ◽  
Semen Extender ◽  
Fresh Semen ◽  
Artificial Vagina

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams.   Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively.  Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%)  was significantly (P<0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%).   Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%).  In conclusion, he addition of sucrose in semen extender  improved the quality of frozen semen of Garut ram.  Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.

scholarly journals Spermatozoa Abnormality Test of Boer Goats Frozen Semen with the Addition of Sweet Orange Essential Oil and Streptomycin in Tris Yolk Extender

2020 ◽  
pp. 20-23
Author(s):  
Sukma Aditya Sitepu
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Egg Yolk ◽  
Boer Goat ◽  
Average Value ◽  
Frozen Semen ◽  
Before And After ◽  
Boer Goats ◽  
Fresh Semen ◽  
Orange Essential Oil

This research aims to to determine the percentage value of spermatozoa abnormalities in Boer Goat frozen semen by adding a combination of streptomycin with sweet orange essential oil to tris yolk extender. The ingredients used in this study were fresh semen from Boer Goat, tris yolk extender, streptomycin, and sweet orange essential oil. Tris yolk extender is made using Tris (hydroxymethylaminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquabides (100 ml). The study design was a non factorial completely treatments given were the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1%. The results showed that the addition of a combination of streptomycin and sweet orange essential oil into the tris yolk extender had a highly significant effect (P <0.01) on the percentage value of spermatozoa abnormalities of Boer Goats before and after freezing. The best average value of spermatozoa abnormalities is the addition of sweet orange essential oil at 1% (P4) with the percentage of spermatozoa abnormalities before freezing at 3% and after freezing at 6%.

Daya Motil dan Keutuhan Membran Plasma Spermatozoa Domba Garut (Ovis aries) Pada Penambahan Kolesterol Dalam Pengencer Semen Tris Kuning Telur

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Herdis
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Egg Yolk ◽  
Ovis Aries ◽  
Motile Sperm ◽  
Quality Of Control ◽  
Cholesterol Treatment ◽  
Frozen Semen ◽  
Artificial Vagina

The research was carried out to study the effect of egg-yolk consentration and cholesterol suplementationto tris - 1.2% maltosa extender on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature Garut rams. Semen was equilibrated at 5oC for three hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. Percentages of motility and intact plasma membrane (IPM) parameters were evaluated every stage of freezing semen. The results show that there were no interaction between both treatmenst on the quality of garut rams frozen semen. In the cholesterol treatment, percentages of progressive motile sperm and intact plasma membrane in control (50,50% and 57,92%) were significantly different (P<0,05) than cholesterol 1,0 mg /100 ml treatment (37,08% and 49,42%) respectively. In conclusion, addition of 10% egg yolk concentrationto Tris –1.2% maltosa extender was not significantly different than 20% egg yolk concentration. The frozen semen quality of control produced the best quality semen compared toboth addition of 0.5mg/100 ml and 1.0 mg/100 ml cholesterol treatments. Cholesterol addition to egg yolk Tris – 1.2% maltosa causes degradation of frozen semen quality.Penelitian dilakukan untuk melihat pengaruh konsentrasi kuning telur dan penambahan kolesterol pada pengencer semen Tris dengan maltosa 1,2% terhadap kualitas semen beku domba garut. Semen dikoleksi seminggu sekali dengan vagina buatan dari enam domba garut jantan. Semen diekuilibrasi pada suhu 5 oC selama tiga jam kemudian dibekukan dan disimpan di dalam nitrogen cair. Metode thawing dilakukan pada suhu 30oC selama 30 detik. Parameter persentase motilitas dan membran plasma utuh dievaluasi pada setiap tahap pembekuan. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara kedua perlakuan terhadap kualitas semen beku domba garut. Perlakuan kolesterol menunjukan bahwa persentase motilitas dan membran plasma utuk pada perlakuan kontrol (50,50% dan 57,92%) lebih tinggi dan berbeda nyata dibandingkan perlakuan1,0 mg/100ml kolesterol (37,08% dan 49,42%). Penambahan kuning telur 10% pada pengencer Tris dengan maltosa 1,2% menghasilkan kualitas semen tidak berbeda dengan penambahan kuninf telur 20%, Penelitian menyimpulkan bahwa kualitas semen beku terbaik diperoleh pada perlakuan kontrol lebih tinggi dibandingkan perlakuan kolesterol 0,5 mg/100 ml dan 1,0 mg/100 ml. Penambahan kolesterol akan menurunkan kualitas semen beku domba garut.Keywords: cholesterol, motility, membrane plasm, garut ram, spermatozoa.

scholarly journals Penambahan alfa-tokoferol dalam pengencer susu skim - kuning telur terhadap kualitas spermatozoa domba Sapudi yang disimpan pada suhu 5°C

2020 ◽  
Vol 9 (3) ◽  
pp. 69
Author(s):  
Satya Alysa Cahya Puspita ◽  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Abdul Samik ◽  
Indah Norma Triana ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Alpha Tocopherol ◽  
Control Treatment ◽  
Control Group ◽  
Cold Temperatures ◽  
Fresh Semen

Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to determine the effect of alphatocopherol supplementation in skim milk-egg yolk extender on viability, motility, and plasma membrane integrity of Sapudi ram spermatozoa. Fresh semen derived from Sapudi ram was divided into four treatment groups. Control treatment (P0): semen was added in the extender of skim milkegg yolk without alpha-tocopherol. Three other treatments: P1, P2, and P3 semen were added in skim milk-egg yolk extender with the supplementation of 0.25, 0.5, and 1 gram alpha-tocopherol/ 100 mL extender, respectively. The results showed that the viability, motility, and integrity of the spermatozoa plasma membrane decreased gradually according to the storage length. Supplementation of skim milk-egg yolk extender with 0.5 gram of alpha-tocopherol/100 mL (P2) was able to maintain spermatozoa quality longer (p <0.05) than the control group. It can be concluded that alpha-tocopherol with a concentration of 0.5 g/100 mL of skim milk-egg yolk extender effectively maintains the quality of Sapudi ram spermatozoa in storage at 5 ° C.

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

Kriopreservasi Semen Kambing Boer dengan Konsentrasi Pengencer Nira Aren dan Gliserol Berbeda

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Muhammad Riyadhi ◽  
Anis Wahdi ◽  
Muhammad Rizal
Keyword(s):  
Sperm Motility ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Boer Goat ◽  
Palm Juice ◽  
Sperm Membrane ◽  
Live Sperm

ABSTRAK                                                                        Penelitian bertujuan untuk mengetahui efektivitas nira aren sebagai pengencer alternatif dalam proses pembekuan (kriopreservasi) semen kambing boer.Kriopreservasi semen kambing boer menggunakan pengencer tris-gliserol-kuning telur (P1 73-7-20%), nira aren-gliseol-kuning telur(masing-masing P2 74-6-20%, P3 73-7-20%, dan P4 72-8-20%) dan andromed (P5 tanpa mengandung kuning telur dan gliserol). Parameter evaluasi meliputi motilitas, viabilitas, dan membrane plasma utuh setelah pengenceran, ekuilibrasi dan thawing.  Evaluasi motilitas pasca thawing menunjukkan P5 52% berbeda nyata (P<0.05) dengan P1 42%, selanjutnya P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 8%, P3 6% dan P4 12%.  Viabilitas pasca thawing menunjukkan P5 65,4% tidak berbeda nyata (P>0,05) dengan P1 61,8%, akan tetapi P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 26,2%, P3 29,8%, dan P4 34%.  Membran plasma utuh (MPU) pasca thawing menunjukkan P5 66,2% tidak berbeda nyata (P>0,05) dengan P1 65,4%, akan tetapi keduanya berbeda sangat nyata (P<0.05) dengan P2 39%, P3 38%, dan P4 36,2%.  Disimpulkan kriopreservasi semen kambing boer dengan pengencer nira aren dan gliserol pada konsentrasi berbeda belum dapat dipergunakan sebagai sumber bibit berdasarkan standar nasional Indonesia.Kata Kunci : Kambing boer, semen, nira arenABSTRACTThe experiment was conducted to determine the effect of sugar palm juice as alternative extender for cryopreservation process of boer semen.Tris-glycerol-egg yolk (P1 73-7-20%), Sugar palm juice-glyserol-egg yolk (P2 74-6-20%, P373-7-20%, dan P4 72-8-20%), and andromed (P5) used as a extender  in the cryopreservation process of boer semen.  Sperm motility (%), live sperm (%) and sperm membrane integrity (%) were recorded after diluted, equilibration and freeze-thawing.  Result of post thawing motility showed that P5 52% was significantly different (P <0.05) with P1 42%, then P5 and P1 were significantly different (P <0.05) with P2 8%, P3 6% and P4 12%. Viability after thawing showed P5 65.4% was not significantly different (P> 0.05) with P1 61.8%, but P5 and P1 significantly different (P <0.05) with P2 26.2%, P3 29.8 %, and P4 34%. Spermmembrane integrity post-thawing showed P5 66.2% was not significantly different (P> 0.05) with P1 65.4%, but both were very significantly different (P <0.05) with P2 39%, P3 38% and P4 36.2%. Conclusions, sugar palm juice-glycerol-egg yolk with differentconcentrationsineffectively as an alternative extenderin cryopreservation of boer semen.Keywords: boer goat, semen, sugar palm juice

scholarly journals The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation

2020 ◽  
Vol 12 (1) ◽  
pp. 125-131
Author(s):  
Raudhah Mahfudhah ◽  
Kartini Eriani ◽  
Zainal Abidin Muchlisin ◽  
Cut Ruhul Muthmainnah
Keyword(s):  
Plasma Membrane ◽  
Clarias Gariepinus ◽  
Membrane Integrity ◽  
Dna Integrity ◽  
Freezing And Thawing ◽  
Randomized Design ◽  
Fetal Bovine ◽  
Fresh Semen ◽  
Different Doses

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

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