scholarly journals Pengaruh Hormon Asam Indol Asetat yang Dihasilkan Azospirillum sp. terhadap Perkembangan Akar Padi

2016 ◽  
Vol 3 (2) ◽  
pp. 66 ◽  
Author(s):  
Puji Lestari ◽  
Dwi N. Susilowati ◽  
Eny I. Riyanti
Keyword(s):  
Root Growth ◽  
Plant Height ◽  
Root Development ◽  
Close Association ◽  
Rice Variety ◽  
Growth Period ◽  
Nitrogen Application ◽  
Rice Culture ◽  
Iaa Production

<p>Free-living bacteria of the<br />genus Azospirillum live in close association with rice roots.<br />This bacteria produced indole acetic acid (IAA), a plant<br />growth hormon, to the environment. IAA was isolated from<br />cultures of Azospirillum strains and investigated for its effect<br />on root development and plant height of rice variety IR64 in<br />vitro. Rice cultures of variety IR64 were grown in vitro and<br />inoculated with cultures of Azospirilllum. Production of IAA<br />by the bacterium during its growth period in rice culture medium<br />containing different levels of nitrogen was observed.<br />Results of the experiment showed that strains Azospirillum<br />Az15 and Az44 had a high ability to produce IAA, i.e., 57.93<br />μg/ml at 12 days after incubation (DAI) and 40.42 μg/ml at 7<br />DAI, respectively. The IAA production pattern of Azospirillum<br />Az15 and Az44 in the liquid medium were fluctuative<br />until the end of the incubation period, while that of the strain<br />Az7 was linier. Strain Az7 gave a better effect on the root<br />development and plant height than strains Az15 and Az44.<br />Treatment combination of strain Az7 and 100% nitrogen gave<br />highest root development. High level of nitrogen increased<br />IAA content in the uninoculated culture, while low IAA<br />content on the inoculated one. Inoculation the culture with<br />strain Az7 together with 50% nitrogen application resulted in<br />the IAA content, root dry weight, root length, fiber root number,<br />and plant height as high as those on cultures containing<br />100% nitrogen (1 mM NH4NO3) without inoculation. Inoculation<br />of rice culture with Azospirillum is expected to reduce<br />nitrogen application on rice IR64 by the IAA production as<br />indicated by significant changes in the root growth and<br />development. A higher concentrations of IAA tend to give<br />better effects on the root growth and development of rice<br />IR64.</p>

scholarly journals In vitro rooting of Crocosmia × crocosmiiflora ‘Lucifer’

2017 ◽  
Vol 29 (1) ◽  
pp. 3-10
Author(s):  
Marcelina Krupa-Małkiewicz ◽  
Piotr Żurawik
Keyword(s):  
Plant Height ◽  
Root Development ◽  
Root Morphology ◽  
Vegetation Period ◽  
In Vitro Cultures ◽  
Affected Plant ◽  
Positive Effect ◽  
Initial Research

Abstract The aim of this study was to determine the effect of various auxins and their concentrations on Crocosmia × crocosmiiflora ‘Lucifer’ rooting in in vitro cultures and in vivo conditions. The initial research materials were shoots obtained from a stabilized in vitro culture. MS media were supplemented with the following auxins: IAA, IBA and NAA at concentrations of 0.5, 1.0, 2.0 and 5.0 mg dm-3. The results of the study showed that hormone applications had a positive effect on the root development of Crocosmia microshoots. 100% rooted shoots were observed in each of the applied combinations of the experiment. The highest number of roots and the longest roots were formed in the presence of IBA at a concentration of 1.0 mg dm-3. IAA, IBA and NAA affected plant height and root morphology of Crocosmia differently. The addition of higher concentrations of auxins to the medium (2.0 and 5.0 mg dm-3) resulted in the formation of short roots. The applied IBA concentrations determined the percentage of the plants that were adapted to in vivo conditions, as well as their vegetative features and the yield of descendant tubers. At the end of the vegetation period, higher IBA concentrations, i.e., 1.0, 2.0 and 5.0 mg dm-3, caused a decrease of up to 50-75% in the obtained plants and also inhibited plant height, as well as the weight and diameter of the tubers formed, compared to the control.

Effects of Growth-period Type and Nitrogen Application Level on the RVA Profile Characteristics for Japonica Rice Genotypes

2013 ◽  
Vol 38 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Min LI ◽  
Hong-Cheng ZHANG ◽  
Guo-Ye LI ◽  
Qun MA ◽  
Xiong YANG ◽  
...  
Keyword(s):  
Growth Period ◽  
Nitrogen Application ◽  
Rice Genotypes ◽  
Profile Characteristics

Diabetes and Heart Failure: Is it Hyperglycemia or Hyperinsulinemia?

2020 ◽  
Vol 18 (2) ◽  
pp. 148-157 ◽  
Author(s):  
Triantafyllos Didangelos ◽  
Konstantinos Kantartzis
Keyword(s):  
Heart Failure ◽  
Insulin Treatment ◽  
Close Association ◽  
Adverse Outcomes ◽  
Negative Effects ◽  
Beneficial Effects ◽  
Appropriate Treatment ◽  
Increased Risk ◽  
Treatment Of Diabetes

The cardiac effects of exogenously administered insulin for the treatment of diabetes (DM) have recently attracted much attention. In particular, it has been questioned whether insulin is the appropriate treatment for patients with type 2 diabetes mellitus and heart failure. While several old and some new studies suggested that insulin treatment has beneficial effects on the heart, recent observational studies indicate associations of insulin treatment with an increased risk of developing or worsening of pre-existing heart failure and higher mortality rates. However, there is actually little evidence that the associations of insulin administration with any adverse outcomes are causal. On the other hand, insulin clearly causes weight gain and may also cause serious episodes of hypoglycemia. Moreover, excess of insulin (hyperinsulinemia), as often seen with the use of injected insulin, seems to predispose to inflammation, hypertension, dyslipidemia, atherosclerosis, heart failure, and arrhythmias. Nevertheless, it should be stressed that most of the data concerning the effects of insulin on cardiac function derive from in vitro studies with isolated animal hearts. Therefore, the relevance of the findings of such studies for humans should be considered with caution. In the present review, we summarize the existing data about the potential positive and negative effects of insulin on the heart and attempt to answer the question whether any adverse effects of insulin or the consequences of hyperglycemia are more important and may provide a better explanation of the close association of DM with heart failure.

scholarly journals A low-cost aeroponic phenotyping system for storage root development: unravelling the below-ground secrets of cassava (Manihot esculenta)

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Michael Gomez Selvaraj ◽  
Maria Elker Montoya-P ◽  
John Atanbori ◽  
Andrew P. French ◽  
Tony Pridmore
Keyword(s):  
Root Growth ◽  
Root Development ◽  
Low Cost ◽  
Root Traits ◽  
Suitable Model ◽  
Root Initiation ◽  
Storage Root ◽  
Economic Traits ◽  
Storage Roots ◽  
Storage Root Development

Abstract Background Root and tuber crops are becoming more important for their high source of carbohydrates, next to cereals. Despite their commercial impact, there are significant knowledge gaps about the environmental and inherent regulation of storage root (SR) differentiation, due in part to the innate problems of studying storage roots and the lack of a suitable model system for monitoring storage root growth. The research presented here aimed to develop a reliable, low-cost effective system that enables the study of the factors influencing cassava storage root initiation and development. Results We explored simple, low-cost systems for the study of storage root biology. An aeroponics system described here is ideal for real-time monitoring of storage root development (SRD), and this was further validated using hormone studies. Our aeroponics-based auxin studies revealed that storage root initiation and development are adaptive responses, which are significantly enhanced by the exogenous auxin supply. Field and histological experiments were also conducted to confirm the auxin effect found in the aeroponics system. We also developed a simple digital imaging platform to quantify storage root growth and development traits. Correlation analysis confirmed that image-based estimation can be a surrogate for manual root phenotyping for several key traits. Conclusions The aeroponic system developed from this study is an effective tool for examining the root architecture of cassava during early SRD. The aeroponic system also provided novel insights into storage root formation by activating the auxin-dependent proliferation of secondary xylem parenchyma cells to induce the initial root thickening and bulking. The developed system can be of direct benefit to molecular biologists, breeders, and physiologists, allowing them to screen germplasm for root traits that correlate with improved economic traits.

scholarly journals Ferroptosis contributes to isoflurane-induced neurotoxicity and learning and memory impairment

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Pengfei Liu ◽  
Jing Yuan ◽  
Yetong Feng ◽  
Xin Chen ◽  
Guangsuo Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Learning And Memory ◽  
Memory Impairment ◽  
Close Association ◽  
Dimethyl Fumarate ◽  
Dependent Manner ◽  
Transport Chain ◽  
The Relationship

AbstractFerroptosis is a novel type of programmed cell death, which is different from apoptosis and autophagic cell death. Recently, ferroptosis has been indicated to contribute to the in vitro neurotoxicity induced by isoflurane, which is one of the most common anesthetics in clinic. However, the in vivo position of ferroptosis in isoflurane-induced neurotoxicity as well as learning and memory impairment remains unclear. In this study, we mainly explored the relationship between ferroptosis and isoflurane-induced learning and memory, as well as the therapeutic methods in mouse model. Our results indicated that isoflurane induced the ferroptosis in a dose-dependent and time-dependent manner in hippocampus, the organ related with learning and memory ability. In addition, the activity of cytochrome c oxidase/Complex IV in mitochondrial electron transport chain (ETC) was increased by isoflurane, which might further contributed to cysteine deprivation-induced ferroptosis caused by isoflurane exposure. More importantly, isoflurane-induced ferroptosis could be rescued by both ferroptosis inhibitor (ferrostatin-1) and mitochondria activator (dimethyl fumarate), which also showed effective therapeutic action against isoflurane-induced learning and memory impairment. Taken together, our data indicate the close association among ferroptosis, mitochondria and isoflurane, and provide a novel insight into the therapy mode against isoflurane-induced learning and memory impairment.

scholarly journals Evaluation of Starch Hydrolysis for Glycemic Index Prediction of Rice Varieties

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 101
Author(s):  
Cristiana Pereira ◽  
Regina Menezes ◽  
Vanda Lourenço ◽  
Teresa Serra ◽  
Carla Brites
Keyword(s):  
Glycemic Index ◽  
Food Processing ◽  
Amylose Content ◽  
Rice Variety ◽  
Great Influence ◽  
Starch Hydrolysis ◽  
Rice Starch ◽  
Rice Varieties ◽  
Processing Technologies

Rice consumed as white cooked polished grain has been considered a high glycemic index (GI) food, particularly compared with other starchy foods. However, the GI levels of rice based food can vary among different rice types and food processing technologies. Rice GI variation can be affected by several factors, such as rice variety, the genetic background of rice as well as due to crop edaphoclimatic conditions. The main difference in rice starch composition that influences GI is the amylose content. Besides the chemical composition of rice, the gelatinization characteristics and food processing can also contribute to starch retrogradation, thus increasing the level of resistant starch with a great influence on GI. To understand the glycemic response of rice types differing in amylose and viscosity profiles, four rice samples were analyzed and compared with standard and resistant HI-MAIZE corn starches. An in vitro enzymatic starch hydrolysis procedure was applied to estimate GI. The results indicate substantial differences in the starch hydrolysis of the two corn starches. Starch hydrolysis tended to be more rapid and efficient for ‘Waxy’ and ‘Ceres’ (intermediate-amylose) rice types than for ‘Maçarico’ (high-amylose rice). In addition, the data show that the Maçarico variety has the lowest estimated GI and the highest retrogradation rate compared with ‘Waxy’, ‘Ceres’ and ‘Basmati’ type. The results obtained reinforce the importance of knowing amylose content and viscosity profiles for the prediction of rice glycemic responses.

scholarly journals Performance of hybrid rice grown from separated tillers

2019 ◽  
Vol 17 (4) ◽  
pp. 507-513
Author(s):  
FN Kamarum Munira ◽  
Md. Parvez Anwar ◽  
Sabina Yeasmin ◽  
Md. Harun Or Rashid ◽  
Mst Farzana Rahman ◽  
...  
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Hybrid Rice ◽  
Rice Variety ◽  
Field Laboratory ◽  
Split Plot ◽  
Rice Seeds ◽  
Four Levels ◽  
Plot Design

The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during July to November 2016 to investigate the performance of separated tillers of hybrid rice variety Dhani Gold. The experiment comprised three times of tiller transplantation viz. 3, 4 and 5 week aged tiller seedlings and four levels of number of tiller seedlings transplanted hill-1 viz. 2, 3, 4 and 5 tiller seedlings hill-1 laid out in a split plot design with 3 replications. The highest plant height was obtained when 3-week aged tiller seedlings were transplanted at the rate of 3 tiller seedlings hill-1. The highest number of total tillers hill-1 and number of effective tillers hill-1 were found when 3-week aged tiller seedlings were transplanted with 5 tiller seedlings hill-1. The highest grains panicle-1 was obtained when 4-week aged tiller seedlings were transplanted with 5 tiller seedlings hill-1. The highest grain yield was found when 3-week aged tiller seedlings were transplanted with 5 tiller seedlings hill-1. The lowest grain yield was found when 5-week aged tiller seedlings were transplanted with 2 tiller seedlings hill-1 in hybrid rice variety Dhani Gold. Based on the findings of the present study it may be concluded that 3-week old tiller seedlings of Dhani Gold should be transplanted at the rate of 4 or 5 tiller seedlings hill-1 for higher yield. Thus present study confirms the potentiality of growing hybrid rice from separated tillers in case of unavailability of seedlings or to minimize the seed cost of high value hybrid rice seeds. J Bangladesh Agril Univ 17(4): 507–513, 2019

scholarly journals An optimised GAS-pharyngeal cell biofilm model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Heema K. N. Vyas ◽  
Jason D. McArthur ◽  
Martina L. Sanderson-Smith
Keyword(s):  
Crystal Violet ◽  
Biofilm Formation ◽  
Cell Model ◽  
Matrix Material ◽  
Three Dimensional ◽  
Growth Period ◽  
Optimal Growth ◽  
Abiotic Surfaces

AbstractGroup A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Biofilm formation has been implicated in both pharyngeal and dermal GAS infections. In vitro, plate-based assays have shown that several GAS M-types form biofilms, and multiple GAS virulence factors have been linked to biofilm formation. Although the contributions of these plate-based studies have been valuable, most have failed to mimic the host environment, with many studies utilising abiotic surfaces. GAS is a human specific pathogen, and colonisation and subsequent biofilm formation is likely facilitated by distinct interactions with host tissue surfaces. As such, a host cell-GAS model has been optimised to support and grow GAS biofilms of a variety of GAS M-types. Improvements and adjustments to the crystal violet biofilm biomass assay have also been tailored to reproducibly detect delicate GAS biofilms. We propose 72 h as an optimal growth period for yielding detectable biofilm biomass. GAS biofilms formed are robust and durable, and can be reproducibly assessed via staining/washing intensive assays such as crystal violet with the aid of methanol fixation prior to staining. Lastly, SEM imaging of GAS biofilms formed by this model revealed GAS cocci chains arranged into three-dimensional aggregated structures with EPS matrix material. Taken together, we outline an efficacious GAS biofilm pharyngeal cell model that can support long-term GAS biofilm formation, with biofilms formed closely resembling those seen in vivo.

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