scholarly journals A Comparative Histological Study of Skin in Clarias gariepinus and Oreochromis niloticus

2021 ◽  
Vol 32 (2) ◽  
pp. 90
Author(s):  
Shafia Khairani ◽  
Faisal Fikri ◽  
Agus Purnomo ◽  
Muhammad Thohawi Elziyad Purnama
Keyword(s):  
Oreochromis Niloticus ◽  
Histological Study ◽  
Clarias Gariepinus ◽  
Histological Structure ◽  
Club Cells ◽  
Basement Layer ◽  
Hematoxylin Eosin ◽  
Dermis Layer

This study aimed to evaluate the comparative of histological figures in Clarias gariepinus and Oreochromis niloticus skin. A total of 18 samples were observed and collected the skin preservation. The hematoxylin-eosin staining performed to determine the histological structure. Results showed that there were different structures in the epidermis and dermis layer between C. gariepinus and O. niloticus skin. Club cells showed dominated in the epidermis layer of C. gariepinus. The pigmented cell showed clearly in the basement layer of C. gariepinus compared to O. niloticus. Stratum compactum in O. niloticus also showed adequate compared to C. gariepinus.

scholarly journals STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator))

2016 ◽  
Vol 10 (2) ◽  
pp. 153-156 ◽  
Author(s):  
Hamny Hamny ◽  
Muhammad Iqbal ◽  
Sri Wahyuni ◽  
Mustafa Sabri ◽  
M. Jalaluddin ◽  
...  
Keyword(s):  
Histological Study ◽  
Histological Structure ◽  
Ventral Lobe ◽  
Nacl Solution ◽  
Dorsal Lobe ◽  
Yellow Color ◽  
Monitor Lizard ◽  
Oval Form ◽  
Varanus Salvator ◽  
Hematoxylin Eosin

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45±0.64 cm (dorsal lobe) and 7.00±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15±0.01 g and ventral lobe was 6.35±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.

scholarly journals Comparative Anatomy and Histology of Black Pomfret (Formio niger) and Nile tilapia (Oreochromis niloticus) Kidney

2017 ◽  
Vol 6 (1) ◽  
pp. 9
Author(s):  
Nurul Safitri Apriliani ◽  
Muhammad Jafar Luthfi
Keyword(s):  
Oreochromis Niloticus ◽  
Lymphoid Tissue ◽  
Nile Tilapia ◽  
Comparative Anatomy ◽  
Distal Tubule ◽  
Histological Structure ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Habitat Differences ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Black pomfret and nile tilapia are belonging to order Perciformes. Both fish are live in different habitat. Black pomfret is marine water whereas nile tilapia is in freshwater. The purpose of the study was to determine anatomy and histology of the kidneys structures in both fishes. Histological preparations were done using paraffin method, with <em>Hematoxylin-Eosin</em> (HE) staining. The results, showed that Black pomfret and nile tilapia have y-shape kidney. Nile tilapia has darker red colour and softer texture than black pomfret kidney. Histologically, black pomfret and nile tilapia kidneys have a distal tubule, proximal tubule, glomerulus and lymphoid tissue. Glomerular diameter of nile tilapia (69,22 µm) was larger than pomfret (61,25 µm). It can be concluded that differences between anatomical and histological structure of kidney are affected by habitat differences.

scholarly journals Histological Study of Mudskipper (Periophthalmus gracilis) Gills

2019 ◽  
Vol 2 ◽  
pp. 177-179
Author(s):  
Hikmah Supriyati ◽  
Nurul Safitri Apriliani ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Histological Study ◽  
Gill Filament ◽  
Histological Structure ◽  
Respiratory Organ ◽  
Secondary Lamellae ◽  
Long Time ◽  
Qualitative Descriptive ◽  
Gill Structure ◽  
And Behavior ◽  
Hematoxylin Eosin

Mudskipper belongs to the Gobiidae family which has respiratory adaptation to fits their habitat. Mudskipper has a different gill structure or modification of gills, this different structure allows the mudskipper to survive for a long time outside water. This study aimed to determine the histology of gills and find out whether there is a modification of gills in the mudskipper respiratory organs (Periophthalmus gracilis). Histological preparations were done using paraffin method, stained with Hematoxylin-Eosin. Data analysis was carried out in a qualitative descriptive. The results showed that there is no additional respiratory organ in the mudskipper respiration, whereas the gills have some modifications. The histological structure of mudskipper gills consists of gill arches, arteries, gill filament, primary lamellae, and secondary lamellae. The gills of mudskipper have a different structure from the general fish, which has a thick secondary lamellae with a low amount of density, the shape of the filament are short and bent. This gill structure is a form of adaptation to habitat and behavior to live outside the water in a relatively long time.

Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia

2020 ◽  
Vol 21 (3) ◽  
pp. 61-66
Author(s):  
Ola Hashem ◽  
Viola Zaki ◽  
Rawia Adawy
Keyword(s):  
Molecular Characterization ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Clarias Gariepinus ◽  
Fungal Species ◽  
High Rate ◽  
Fish Farms ◽  
Isolation And Identification ◽  
Penicillium Spp

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

Description of Freshwater Fish Traditional Smoking in the Western Region, Cameroon

2021 ◽  
Vol 14 (1) ◽  
pp. 61-64
Author(s):  
Felix Meutchieye ◽  
Henri Grisseur Djoukeng ◽  
Youssouf Jamilou Ngouyamsa ◽  
Yacouba Manjeli
Keyword(s):  
Ethnic Group ◽  
Freshwater Fish ◽  
Oreochromis Niloticus ◽  
Clarias Gariepinus ◽  
Small Scale ◽  
African Catfish ◽  
Economic Activities ◽  
Fresh Fish ◽  
Hygiene Measures ◽  
Average Quantity

Western Cameroon is one of the regions most in needs of innovative and healthy solutions for freshwater fish conservation. This study aimed at evaluating the socio-economical characteristics of fish smokers and technical factors on the species smoked in the Noun Division. A total of 91 fish smokers were chosen. The socio-economic data were on age, gender, religion, number of dependents, marital status, ethnic group, education level, economic activities, smoking goals and workforce. The results of this study showed that fish smoking is practiced mainly by women (70%) which is common in Sub Sahara Africa small scale fish sector. The smokers are aged from 20 to 50 years (80%), married (92.2%) and taking care of a family of 1 to 10 persons (80%). These smokers are Muslim (74%), belonging to the Bamoun ethnic group (81%). They were also involved in other activities: agriculture (29%) and trade (1%). About 67% of the sampled smokers’ population had an experience of more than 10 years in the domain. The Purpose of smoking was largely sales (97%) and subsistence (3%). Nile tilapia (Oreochromis niloticus) was the most smoked species (36%), followed by African catfish (Clarias gariepinus) with a proportion of 32%. The average quantity of fresh fish smoked was 110 kg/day/smoker, with Oreochromis niloticus being more produced (60±4.74 kg/day/smoker). With regard to the smoking techniques used, hygiene measures were poorly respected. The major constraints revealed by the survey were that freshwater’s traditional fish smoking activities in Noun Division are influenced by gender, economic and cultural background. There is a lack of technical support material and financial means. Any implications for future investigations on health and food safety will be suitable for fresh fish smokers and the entire consumers.

Risk Assessment of Some Heavy Metals in Two Fish Species Oreochromis Niloticus and Clarias Gariepinus from Sharqia Province, Egypt

2021 ◽  
Vol 8 (1) ◽  
pp. 1-5
Author(s):  
Nahla S El-Shenawy ◽  
Heba N Gad EL-Hak ◽  
Mahi A Ghobashy ◽  
Maha FM Soliman ◽  
Farida A Mansour ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Risk Assessment ◽  
Human Health ◽  
Health Risks ◽  
Oreochromis Niloticus ◽  
Clarias Gariepinus ◽  
Human Health Risk ◽  
Dry Weight ◽  
Critical Problem ◽  
Potential Health

Accumulation of heavy metals in fish is considered a critical problem for human health. Therefore, the study aimed to quantify the concentrations of iron (Fe), zinc (Zn), manganese (Mn), and lead (Pb) in Oreochromis niloticus and Clarias gariepinus from two areas in Al Sharqia governorate, Egypt, from September 2017 to August 2018. A human health risk assessment was conducted to evaluate the potential hazards associated with fish consumption. Metals concentrations (mg/kg dry weight) in muscles of catfish ranged 1.88-221.26 for Fe; 1.78-19.77 for Zn; BDL-238.51 for Mn; BDL-22.75 for Pb. In muscles of tilapia fish metals concentrations ranged 7.96-149.10 for Fe; 1.20-19.77 for Zn; BDL-230.82 for Mn; BDL-25.93 for Pb. Pb had Hazard quotients (HQs) which indicated potential health risks to tilapia consumers at both study areas and catfish consumers at the Faqous area. Fishermen were at higher risk compared to the other consumers.

scholarly journals Tracheotomy in growing rats: histological aspects

2003 ◽  
Vol 18 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Mônica Cecília Bochetti Manna ◽  
Edna Frasson de Souza Montero ◽  
Maria Antonieta Longo Galvão da Silva ◽  
Yara Juliano
Keyword(s):  
Inflammatory Infiltrate ◽  
Sham Group ◽  
Histological Study ◽  
Reference Pattern ◽  
Microsurgical Technique ◽  
Growing Rats ◽  
Global Comparison ◽  
Different Types ◽  
Hematoxylin Eosin ◽  
Epidermoid Metaplasia

PURPOSE: To compare morphologically three different types of tracheotomy in growing rats, applying microsurgical technique. METHODS: EPM-1 Wistar growing rats (n=57) weighing 88gm and aged 35 days were randomized in four groups, according tracheotomy incision type (longitudinal, transverse and tracheal segment excision), and sham group. Following intramuscular anesthesia with ketamine and xylazine, the trachea was exposed and incised, according to the group, and a hand-made endotracheal cannula was inserted into the organ, under sterile conditions. This cannula was removed after 7 days, and animals have been sacrificed 30 days later. Tracheas samples were submitted to histological study, stained by hematoxylin-eosin and Masson trichrome, evaluating fibrosis, inflammatory infiltrate and epidermoid metaplasia. RESULTS: There was more frequency of inflammatory infiltrate at the tracheal epithelium in the tracheal segment excision group (87%) compared to the longitudinal (40%) and transverse (36%) incision groups (p=0.009). Evaluating epidermoid metaplasia, tracheal segment excision and the longitudinal groups presented 33% and 40%, respectively, compared to 0% of the transverse group (p=0.03). Concerning to fibrosis, in a global comparison (p=0.1) among the three groups there was no difference, however, compared to the longitudinal group the transverse group showed lower level of fibrosis (p=0.04). Sham group did not present any relevant morphologic alterations and it was used as reference pattern. CONCLUSION: Taken together, our data show that tracheal segment excision promotes more epithelium aggression and transverse tracheal incision shows less morphologic alterations.

scholarly journals Histological analysis of the tongue, esophagus and crop of Southern Carcará (Polyborus plancus)

2021 ◽  
Vol 10 (2) ◽  
pp. e21410212439
Author(s):  
Vanessa Sobue Franzo ◽  
Leiny Paula de Oliveira ◽  
Ana Carolina Pôssas Viana ◽  
Adriano Tony Ramos ◽  
Jéssica Fernanda Bertolino ◽  
...  
Keyword(s):  
Digestive System ◽  
Histological Analysis ◽  
Squamous Epithelium ◽  
Eating Habits ◽  
Histological Study ◽  
Pekin Ducks ◽  
The Family ◽  
Body Weights ◽  
Stratified Squamous Epithelium ◽  
Hematoxylin Eosin

The Southern Caracara is a bird of prey, of the family Falconidae, inhabits all the Brazilian territory, they are omnivorous birds adapted for hunting. The digestive system of the predators presents differences in their morphology due to their eating habits. Knowing the importance of the gastrointestinal system, this study aims to carry out a histological study of the tongue, esophagus and the crop of Polyborus plancus. For this purpose, eight avian of both sexes, with different body weights and ages were used, they were euthanized and the organs were collected, fixed, dehydrated and stained with hematoxylin-eosin. It was noted that the tongue has stratified squamous epithelium with mucous glands and with different degrees of keratinization. The esophagus and crop have four well developed muscle layers and mucous glands. Additionally, in the crop there is presence of an adventitious layer that consists of loose connective tissue. We conclude that the tongue, the esophagus and the crop of the Southern Caracara have similar histological organization of several birds like the Pekin Ducks, ostriches, domestic chicken and partridges.

scholarly journals the Study of some histopathological changes occurring in white laboratory mice infected with Cutaneous Leishmaniasis in Al – diwaniyah province, Iraq.

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Lubna.A.Al-ibrahimi Edu ◽  
Hind Al-shabani ◽  
Marwa sami alwan
Keyword(s):  
Bile Duct ◽  
Mediterranean Region ◽  
Histological Study ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Intracellular Parasite ◽  
Laboratory Mice ◽  
Histopathological Changes ◽  
Radial Arrangement ◽  
Dermis Layer

          Leishmaniasis is caused by an intracellular parasite . It is endemic in Asia, Africa, the Americas, and the Mediterranean region. Worldwide, 1.5 to 2 million new cases occur each year .The histological study of the liver tissue of white laboratory mice (Mus musculus) infected with L. major a parasite showed The presence of severe steatosis of hepatocytes Hepatocyte degeneration, And loss of the radial arrangement of hepatocytes, With heavy infiltration in inflammatory cells, especially phagocytes( Macrophage) with  Hyperplasia and congestion of the bile duct  .  As for histological sections of skin lesions taken from ear , Foot , tail ، were showed epidermal ulcerative , Accompanied by severe leaching of the dermis layer neutrophil ,polymorph lymphocytes ، with hemorrhage of ​​the dermis, with necrosis of the epidermal cells of all skin lesions in the ear, foot and tail.

scholarly journals Morphological and Histological Study of an ‘Iliac Venous Ladder’ Associated with Very Short Common Iliac Arteries

2019 ◽  
Vol 36 (04) ◽  
pp. 255-260
Author(s):  
Satheesha B. Nayak ◽  
Sudarshan Surendran ◽  
Venu Madhav Nelluri ◽  
Prakashchandra Shetty
Keyword(s):  
Developmental Process ◽  
Present Report ◽  
Histological Study ◽  
Vena Cava ◽  
Iliac Vein ◽  
Histological Structure ◽  
Common Iliac Vein ◽  
Structural Variations ◽  
Surgical Interventions ◽  
The Common

Abstract Introduction Communications between iliac veins in the pelvis are reported to be rare occurrences, which are mostly due to developmental abnormalities. The common iliac vein is formed by the joining of the internal and external iliac veins. Here, we present a detailed morphological and histological study of a rare communication found between the internal and external iliac veins, which would prove to be of substantial value to the knowledge of vessels in the pelvis, both for clinicians and surgeons. Materials and Methods In the present study, we came across a rare communication in the form of a ladder, between the left internal and external iliac veins in a 70-year-old male cadaver. There were two communications (named upper and lower communications) between the external and internal iliac veins, before they joined to form the common iliac vein. On naked eye observation, the lumen of the right common iliac vein appeared to be wider than on the left side. The lengths of these communications and the distance between each of their attachments have been measured and tabulated. The walls of these veins, their microscopic sections and their communication involved in the case were taken. The possible causes for these variations were congenital, owing to the complicated nature of the developmental process involved in the formation of the inferior vena cava and the venous system of the lower limb. Results The structure of all the veins involved in this case and the communications were seen to be normal. The thickness of these walls were measured and tabulated for uniformity around the circumference of the wall of these veins. All the measurements from the structural variations and from the histological observations are tabulated in our results. Conclusion The complicated development sequence of these veins could have possibly led to the persistence of these communications. Such a developmental variation does not seem to pose any threat until unless encountered under clinical or surgical interventions, as the histological structure of the walls of the communications as well as the major channels connected appeared to be normal and well-developed. The detailed morphological and histological features of these structures involved in the variation along with the possible complications have been presented in the present report. Knowledge of these variations and complications due to injury plays a key role in a clinical setup.

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