scholarly journals 12. Effectiveness of Jatropha Sap Oinment 10% (Jatropha curcas Linn.) and Gentamicin Ointment 0.1% on Combustion Injury II Healing on Mice Skin (Mus musculus) (EFEKTIVITAS SALEP GETAH JARAK PAGAR 10% (Jatrophacurcas Linn.) DAN GENTAMISIN 0,1% TERHADAP PERCEPATAN PENYEMBUHAN LUKA BAKAR DERAJAT II PADA KULIT MENCIT (Mus musculus))

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Rodi Jasmadi
Keyword(s):  
Wound Healing ◽  
Key Words ◽  
Analysis Of Variance ◽  
Mus Musculus ◽  
Jatropha Curcas ◽  
Healing Process ◽  
Healing Time ◽  
Treatment Groups ◽  
Significant Difference ◽  
Difference Test

This research was aimed to determine the effectiveness of second degree combustion wound healing after the administration of Jatropha (Jatropha curcas Linn.) sap oinment 10% and Gentamicin ointment 0.1% on mice skin (Mus musculus). This research used 12 mice divided into 3 treatment groups: yellow vaseline lubrication (K0), jatropha sap oinment 10% (KI), and ointment Gentamicin 0.1% (KII). The parameter measured in this study was healing time (day) based on wound not visible/healed (diameter length=0 cm). Data of wound healing were analyzed using analysis of variance (Anova) and continued with least significant difference test. The time of wound healing on P0, P1, and P2 was 22.25, 17.75, and 20.25 days (P0.01). In conclusion, sap of jatropha oinment 10% is potensial to accelerate the combustion injury II healing process in mice. ____________________________________________________________________________________________________________________ Key words: wound, yellow vaseline, jarak pagar secretion 10%, gentamicin 0.1 %

scholarly journals Efek Serbuk Kunyit dan Kurkumin pada Spermatogenesis Mencit (Mus musculus) yang Diberi Minuman Beralkohol

2021 ◽  
Vol 6 (2) ◽  
pp. 154-160
Author(s):  
Helena Chika Valencia Hanisa ◽  
Tyas Rini Saraswati ◽  
Silvana Tana
Keyword(s):  
Analysis Of Variance ◽  
Mus Musculus ◽  
Alcoholic Beverages ◽  
Seminiferous Tubules ◽  
Treatment Groups ◽  
Alcohol Control ◽  
Randomized Design ◽  
Testicular Weight ◽  
Significant Difference ◽  
Spermatogonia Cells

Kunyit mengandung senyawa kurkumin yang dapat digunakan sebagai zat antiinflamasi dan membantu memperbaiki sel-sel yang rusak. Tujuan dari penelitian ini menganalisis pengaruh serbuk kunyit dan kurkumin pada jumlah dan ukuran sel spermatogonium; spermatosit primer; dan spermatosit sekunder; bobot testis serta diameter tubulus seminiferus Mus musculus yang diberi minuman beralkohol. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 12 ekor Mus musculus jantan yang dibagi kedalam 4 kelompok perlakuan dan 3 kali ulangan. R0 merupakan kontrol, R1 kontrol alkohol, R2 pemberian serbuk kunyit sebanyak 0,1 mg/hari, R3 pemberian kurkumin sebanyak 0,01 mg/hari. Perlakuan diberikan selama 30 hari. Data penelitian dianalisis menggunakan analysis of variance (ANOVA) pada taraf kepercayaan 95%. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa tidak terdapat perbedaan bermakna (p>0.05) pada jumlah spermatogonium dan ukuran sel (spermatogonium, spermatosit primer, dan spermatosit sekunder), namun terdapat perbedaan bermakna pada (P<0,05) pada bobot testis, diameter tubulus seminiferus dan jumlah sel (spermatosit primer, dan spermatosit sekunder). Turmeric contains curcumin compounds that can be used as anti-inflammatory substances and help repair damaged cells. The purpose of this study was to analyze the effect of turmeric powder and curcumin on the number and size of spermatogonia cells; primary spermatocytes; and secondary spermatocytes; testicular weight and diameter of the seminiferous tubules of Mus musculus given alcoholic beverages. This study is an experimental study with a completely randomized design (CRD), using 12 male Mus musculus which were divided into 4 treatment groups and 3 replications. R0 is control, R1 is alcohol control, R2 is 0.1 mg/day of turmeric powder, R3 is 0.01 mg/day of curcumin. The treatment was given for 30 days. The research data were analyzed using analysis of variance (ANOVA) at the 95% confidence level. Based on the results obtained, it can be concluded that there is no significant difference (p>0.05) in the number of spermatogonia and cell size (spermatogonia, primary spermatocytes, and secondary spermatocytes), but there is a significant difference (P<0.05) in testicular weight, diameter of the seminiferous tubules and the number of cells (primary spermatocytes, and secondary spermatocytes).

scholarly journals Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50

2014 ◽  
Vol 13 (2) ◽  
pp. 86
Author(s):  
Hendrik Setia Budi ◽  
Ira Arundina ◽  
Retno Indrawati ◽  
Leonita Widyana Mahardikasari
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Toxicity Test ◽  
Control Group ◽  
Treatment Groups ◽  
Musa Paradisiaca ◽  
Significant Difference ◽  
Post Test ◽  
Stem Extract ◽  
Banana Stem

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.

scholarly journals Extract of haruan (Channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Devintha Ayu Mellyana Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.</p>

scholarly journals Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 224
Author(s):  
Hardany Primarizky ◽  
Wiwik Misaco Yuniarti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Ellagic Acid ◽  
Healing Process ◽  
Control Group ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Significant Difference ◽  
Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05).  From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

scholarly journals Extract of haruan (channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Devintha AM. Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.

scholarly journals Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 226
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p&lt;0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals PEMANFAATAN KOMPOS UNTUK MENINGKATKAN HASIL STROBERI ORGANIK DI KOTA TOMOHON

EUGENIA ◽  
2019 ◽  
Vol 22 (3) ◽  
Author(s):  
J. M.J. Supit ◽  
Y. E.B. Kamagi ◽  
W. Kumolontang
Keyword(s):  
Data Analysis ◽  
Key Words ◽  
Analysis Of Variance ◽  
Agricultural Land ◽  
Chicken Manure ◽  
Randomized Design ◽  
Significant Difference ◽  
Significant Yield ◽  
Completely Randomized Design ◽  
Difference Test

ABSTRACT  Study on compost utilization to support organic strawberry production in Tomohon has been performed to promote the use of compost (mixture of chicken manure and rice husk) in organic strawberry and to determine the compost dosage producing highest strawberry yield. Completely randomized design (CRD) with 4 (four) replicates  has been performed to apply various compost levels, namely 60 t/ha (SPo), 60 t/ha (SP1), 120 t/ha (SP2), 180 t/ha (SP3), and 240 t/ha (SP4). Data analysis has been conducted by means of analysis of variance (ANOVA) followed by the Least Significant Difference Test.  The results revealed the significant yield differences among treatments.  The highest result (28 fruits/ pot having 174.64 gram weight) was found in SP3 compost level (180 t/ha).Key words: compost, agricultural land Rurukan Tomohon, strawberries

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Channa Striata ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.</p><p>Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.</p>

scholarly journals Tekelan Leaves (Chromolaena odorata) Infusion and 10% Povidone-Iodine on Incision Wound Healing Process of Mice (Mus musculus) Infected with Staphylococcus aureus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arif Caesar Budi ◽  
Iwan Sahrial Hamid ◽  
Djoko Legowo
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Mus Musculus ◽  
Povidone Iodine ◽  
Healing Process ◽  
Wound Healing Process ◽  
Skin Area ◽  
Chromolaena Odorata ◽  
Synthetic Drugs ◽  
Significant Difference

Skin is considered to be the most prone organ to injury and infection compared to other body organs. Skin infections are most commonly caused by Staphylococcus aureus. The most commonly used synthetic drugs to overcome skin problems is Povidone-iodine. Considering the Indonesian traditional method, injuries could be cured by dripping Tekelan leaves (Chromolaena odorata) infusion on the wounded skin area. This study aimed to compare the treatment effects of Tekelan leaves (Chromolaena odorata) infusion and povidone-iodine in the wound healing process of mice skin. The subjects of this study were 25 mice (Mus musculus) which were incised and infected with Staphylococcus aureus in 5 groups, namely, P0 (without treatment), P1 (povidone-iodine 10%), P2 (Tekelan infusion 5%), P3 (Tekelan infusion 10%), and P4 (Tekelan infusion 20%). Observations were made microscopically on wound conditions based on a total assessment of the four observed parameters (epithelization, inflammation rate, connective tissue proliferation, and angiogenesis). The results showed that the total observation value in the P0 and P2 groups was lower than the other groups. The P1 group obtained the highest score. The Kruskal-Wallis test showed a significant difference among the treatment groups (p < 0.05). While the Mann Whitney test indicated P1 had a significant difference with P0, P2, and P3, it had no significant difference with P4. It can be concluded that the Tekelan Leaves (Chromolaena odorata) infusion can be used as an alternative of povidone-iodine with an optimal concentration of 20%.

scholarly journals The Additional of Soil Conditioner Substances to Accelerate Ectomycorrhiza Colonization and Growth of Shorea javanica

2018 ◽  
Vol 6 (1) ◽  
pp. 16
Author(s):  
Andreas Kusuma ◽  
Melya Riniarti ◽  
Surnayanti Surnayanti
Keyword(s):  
Key Words ◽  
Root System ◽  
Analysis Of Variance ◽  
Soil Condition ◽  
Soil Conditioner ◽  
Significant Difference ◽  
Colonization Process ◽  
The Media ◽  
Difference Test

Shorea javanica is family of Dipterocarpacae that could associate with ectomycorrhiza.  Ectomycorrhiza colonization were influenced by many factors, such as the soil condition. The purpose of this research were to know the proper concentration of soil conditioner substances to increase growth and accelerate ectomycorrhiza colonization process. This research was done in May to August 2016 by using Randomized Complete Design. Ectomycorrhiza used was suspension spore of Scleroderma columnare 20 ml/polybag. With different treatment of the concentration of Bio-Nature 50 (BN50) addition and given as much as 20 ml / polybag which were a) no added ectomycorrhiza and BN50, b) added ectomycorrhiza inoculum, c) added ectomycorrhiza inoculum and 0,1 % BN50, d) added ectomycorrhiza inoculum and 0,2 % BN50, and e) added ectomycorrhiza inoculum with 0,3.% BN50. Data were analyzed using analysis of variance followed by Least Significant Difference test. The experimantal results showed that additional 0,1 % concentration of BN50 could increase growth of S. javanica. The additional of BN50 0,1 %, 0,2 % and 0,3 % in the media that have been inoculated give equally good results in accelerated ectomycorrhiza colonization on root system of S. javanica. Key words : Bio-Nature 50, ectomycorrhiza, Scleroderma columnare, Shorea javanica, soil conditioner.

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