scholarly journals Study of hemolytic activity and subfactors of complement in smokers intoxication

2019 ◽  
Author(s):  
Rojan Ghanim Al Allaf
Keyword(s):  
Hemolytic Activity ◽  
Normal Values ◽  
Control Group ◽  
Healthy Individuals ◽  
Complement Components ◽  
Complement Activity ◽  
Normal People ◽  
Hemolytic Complement Activity ◽  
Heavy Smokers

Abstract Heavy Smokers appeared to be less resistant to infection, such as bacteria, viruses, and parasites. Many studies have examined the complement components concentrations than compared with normal people and ignored the functional sequencing of complement components, our study included the determination of complement activity by using Sheep red blood cells (SRBCs) as antigen and extracting the hemolytic activity (50%) of complement compounds, and because of difficulty of this method we using statistical analysis program (SPSS 23) and derived the inverse equation which gives the decomposition percentage (1-100%) of complement components by using five serum dilutions only. The total hemolytic complement activity (CH50) and its C3 and C4 fractions were determined in 30 heavy smokers. The results were compared with a control group that contained 30 persons matched in age and sex. Generally, both C3 and C4 concentrations were increased in smoker's individual in compared with the control group. However, when the independent t-test has applied the differences in the C3 and C4 levels in the control group (healthy individuals) and in the smoker group were found to be statistically insignificant but the inverse equation showed a 7% reduction in CH50 in smokers compared with the control group, where 18% reduction was observed. Our current study suggests that the complement components of the heavy smokers suffer from a significant dysfunction in the function, although the concentration of the basic components in the serum is parallel with normal values.

scholarly journals Study of hemolytic activity and subfactors of complement in smokers intoxication

2019 ◽  
Author(s):  
Rojan Ghanim Al Allaf
Keyword(s):  
Hemolytic Activity ◽  
Normal Values ◽  
Control Group ◽  
Healthy Individuals ◽  
Complement Components ◽  
Complement Activity ◽  
Normal People ◽  
Hemolytic Complement Activity ◽  
Heavy Smokers

Abstract Background: Heavy Smokers appeared to be less resistant to infection, such as bacteria, viruses, and parasites. Many studies have examined the complement components concentrations than compared with normal people and ignored the functional sequencing of complement components. Methods: our study included the determination of complement activity by using Sheep red blood cells (SRBCs) as antigen and extracting the hemolytic activity (50%) of complement compounds, and because of difficulty of this method we using statistical analysis program (SPSS 23) and derived the inverse equation which gives the decomposition percentage (1-100%) of complement components by using five serum dilutions only, The total hemolytic complement activity (CH50) and its C3 and C4 fractions were determined in 30 heavy smokers. Results: The results were compared with a control group that contained 30 persons matched in age and sex. Generally, both C3 and C4 concentrations were increased in smoker's individual in compared with the control group. However, when the independent t-test has applied the differences in the C3 and C4 levels in the control group (healthy individuals) and in the smoker group were found to be statistically insignificant but the inverse equation showed a 7% reduction in CH50 in smokers compared with the control group, where 18% reduction was observed. Conclusions: Our current study suggests that the complement components of the heavy smokers suffer from a significant dysfunction in the function, although the concentration of the basic components in the serum is parallel with normal values.

Determination of normal values for an isocapnic hyperpnea endurance test in healthy individuals

2016 ◽  
Vol 230 ◽  
pp. 5-10 ◽  
Author(s):  
Marjolaine Vincent ◽  
Isabelle Court-Fortune ◽  
Clément Brun ◽  
Jean-Philippe Camdessanché ◽  
Samuel Vergès ◽  
...  
Keyword(s):  
Normal Values ◽  
Healthy Individuals ◽  
Endurance Test ◽  
Isocapnic Hyperpnea

Determination of Soluble MICA in Serum as a Novel Marker for Tumor Stage and Metastasis.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1344-1344
Author(s):  
Helmut R. Salih ◽  
Petra Stieber ◽  
Andrea Peterfi ◽  
Dorothea Nagel ◽  
Lothar Kanz ◽  
...  
Keyword(s):  
T Cells ◽  
Differential Diagnosis ◽  
Nk Cells ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Cd8 T Cells ◽  
Control Group ◽  
Healthy Individuals ◽  
Benign Disorders

Abstract The human NKG2D ligands (NKG2DL) MICA and MICB have been shown to be expressed on tumors of epithelial and hematopoietic origin in vivo. Recently we reported that MICA is shed from the cell surface of tumor cells and is present in sera of tumor patients (J Immunol169:4098 (2002), Blood102:1389 (2003)). Since the strength of an anti-tumor response by NK cells and CD8 T cells is critically depending on NKG2DL expression levels, down-regulation of MICA-expression on tumor cells represents an immune escape mechanism that diminishes anti-tumor reactivity of NKG2D-bearing lymphocytes. However, no data are yet available regarding the correlation of soluble MICA (sMICA) levels with specific tumor entities, aggressiveness of the disease, and hence the potential implementation of sMICA as novel marker in differential diagnosis and prognosis of cancer. In this study, we determined sMICA levels in sera of 512 individuals including 296 patients with various cancers, 154 patients with benign disorders and 62 healthy individuals. Healthy individuals revealed significantly lower sMICA values (median<30pg/mL) than patients with benign diseases (84pg/mL; p=0.005) and cancer patients (161pg/mL; p<0.0001). In addition, sMICA levels differed significantly between cancer patients and patients with benign disorders (p<0.0001) that represent the most relevant control group for differential diagnosis. In cancer patients, while there was no association between sMICA levels and tumor size (p=0.456), cell differentiation (p=0.271), or lymph node involvement (p=0.674), sMICA correlated significantly with cancer stage and metastasis (p=0.015 and p=0.007, respectively). Our data indicate that release of MICA might play a role in late stages of tumor progression by overcoming the confining effect of NK cells and CD8 T cells. Thus, determination of sMICA levels provides valuable information for cancer staging, and sMICA in serum seems to be an indicator for systemic manifestation of malignancy rather than for local tumor extent.

scholarly journals EPITHELIAL CELLS EXPRESSING TLR-2 LEVEL AND NOSE MUCOSA BIOCENOSIS IN ALLERGIC RHINITIS PATIENTS

2013 ◽  
Vol 10 (6) ◽  
pp. 20-24
Author(s):  
Yu A Tyurin ◽  
A A Sharifullina ◽  
I G Mustafin ◽  
R S Fassakhov
Keyword(s):  
Allergic Rhinitis ◽  
Epithelial Cells ◽  
Healthy Volunteers ◽  
Nasal Mucosa ◽  
Allergic Diseases ◽  
Nasal Lavage ◽  
Control Group ◽  
Healthy Individuals ◽  
Local Immunity

Background. Determination of local epithelial cells expressing TLR2 as an indicator of local immunity in allergic rhinitis (AR) patients with opportunistic species of staphylococci nasal mucosa colonization. Methods. Washed epithelium samples obtained from patients with seasonal AR (n=8) aged 19—42 years, and perennial AR (n=15) aged 19 —45 years, as well as a control group (20 patients) aged 19—45 years without allergic diseases were investigated. Epithelial cells expressing TLR2 receptors were determined by flow cytometry. Results. The level of epithelial cells expressing TLR2 receptor in patients with seasonal AR was almost in 1,9 times, in perennial AR group — in 1,7 times lower then in healthy individuals. In patients with perennial AR S. aureus was obtained in 96,0% (CI: 79,5—99,2), in association with Str. pyogenes in 29% (CI: 14,9—49,2), Neisseria spp. — in 63,0% (CI: 42,7—78,8). Seasonal allergic rhinitis was characterized by association of S. aureus and S. hemolyticus (70,0%, 44,4—85,8). Conclusion. Ratio of epithelial cells positive for TLR2 in nasal lavage from patients with AR was lower than in healthy volunteers. Indicators proportion of epithelial cells expressing TLR2 in nasal lavage in patients with seasonal AR during an exacerbation period was significantly reduced (1,7—1,9 times), in comparison with healthy volunteers. In AR patients with increased density of staphylococci strains in nasal mucosa increased local epithelial cells expressing TLR2 in nasal lavage was established.

Automated homogeneous liposome-based assay system for total complement activity

1995 ◽  
Vol 41 (4) ◽  
pp. 586-590 ◽  
Author(s):  
S Yamamoto ◽  
K Kubotsu ◽  
M Kida ◽  
K Kondo ◽  
S Matsuura ◽  
...  
Keyword(s):  
Serum Proteins ◽  
M Protein ◽  
Homogeneous Population ◽  
Complement Activity ◽  
Activity Test ◽  
Human Sera ◽  
Total Complement ◽  
Hemolytic Complement Activity ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract We developed an automated homogeneous immunoassay, based on immune lysis of dinitrophenyl (DNP)-labeled liposomes, for measuring total complement activity. Liposome lysis caused by complement activity was detected spectrophotometrically from entrapped glucose-6-phosphate dehydrogenase activity. Complement activity in human sera was quantified by comparison with a calibration curve. For ease of application to fully automated routine clinical analyzers, we adopted a two-reagent system, one reagent containing a homogeneous population of small DNP-labeled liposomes and one containing antibody/substrate. This system required calibration only once a week. Within-run and between-run CVs were 0.4-1.3% (n = 10) and 1.8-4.7% (n = 10), respectively. Serum results were linear upon dilution (with saline) over a twofold range. Bilirubin, hemoglobin, Intrafat, and serum proteins such as rheumatoid factor, M protein, IgG, and IgA did not affect the assay results. The results (y) correlated well with those from a hemolytic complement activity test (x): y = 1.05x - 1.14, r = 0.92, on 66 samples in the range &lt; 10- &gt; 50 kU/L. This method should therefore be of great use for the determination of complement activity.

scholarly journals AB0327 SERUM IRISIN LEVELS IN HEALTHY WOMEN AND PATIENTS WITH RHEUMATOID ARTHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1462.1-1462
Author(s):  
A. Yury ◽  
В. Zavodovsky ◽  
P. J ◽  
S. L ◽  
E. Papichev ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Normal Level ◽  
Normal Values ◽  
Reference Interval ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Individuals ◽  
Average Value ◽  
Healthy Female ◽  
Group 2

Objectives:to study serum irisin levels in healthy females and patients with rheumatoid arthritis.Methods:We examined 110 patients with a reliable diagnosis of rheumatoid arthritis (RA). The age of the examined was from 18 to 69 years, all patients were female. The diagnosis of RA was established on the basis of the 2010 EULAR diagnostic criteria. The group of patients included patients with a diagnosis at least one month before the planned screening. As a control group, as well as to create a representation of the normal values irisina level in the blood serum of healthy persons were examined 60 healthy volunteers (all women). In both groups, the level of serum irisin was determined using the enzyme-linked immunosorbent assay by the commercial Irisin ELISA kit.Results:As a result of measurements in the group of healthy individuals, the average value with the standard deviation used to assess the reliability of the average values was 20.49±4.82 μg/ml (μ±σ). By calculation, a reference interval of 10.85-30.13 μg/ml was determined, defined as μ±2σ. In patients with RA, the level of serum irisin was 14.52±6.99 μg / ml (μ±σ), which is significantly lower than in healthy individuals (p<0.01). Then we divided all patients into 2 groups: group 1 with normal values (66 people), group 2 (44 patients) - with a reduced (less than 10.85 μg/ml) level of irisin. In both groups, the dynamics of the level of serum irisin was studied depending on the duration of the disease. Among patients with a disease RA duration of less than 4 years, 16 (24.24%) patients had a normal level of irisin, and 14 (31.82%) had a reduced level (less than 10.85 μg/ml). Among patients with a disease duration of more than 10 years, 36 (54.55%) patients had a normal level of serum irisin, and a low level was determined in 16 (36.36%) patients (χ2=3.568, p=0.168).Conclusion:According to the data obtained, the normal level of serum irisin in healthy female individuals was 10.85-30.13 μg/ml. In patients with RA, the average level of irisin is significantly lower, however, with an increase in the duration of the disease, it tends to normalize.Disclosure of Interests:None declared

scholarly journals ASSESSMENT OF THE LEVEL OF ALPHA-2 MICROGLOBULIN OF FERTILITY AT THE SYSTEMIC AND TISSUE LEVELS AS AN INDICATOR OF THE FUNCTIONAL STATE OF THE ENDOMETRIUM

2018 ◽  
pp. 26-30
Author(s):  
Yu. A. Lyzikova ◽  
N. M. Golubykh ◽  
A. E. Kozlov
Keyword(s):  
Normal Values ◽  
Endometrial Tissue ◽  
Reproductive Age ◽  
Control Group ◽  
Study Group ◽  
Reproductive Dysfunction ◽  
Female Patients ◽  
Estradiol Concentration ◽  
Concentration Levels

Objective: to determine the level of alpha-2 microglobulin of fertility in the serum and endometrium of female patients of reproductive age. Material and methods . 30 female patients of reproductive age were included in the study. According to the results of clinical and case history data, 20 (66.67 %) patients with reproductive dysfunction were included in the study group, 10 (33.33 %) healthy patients were included in the control group. The concentration levels of hormones (FSH, LH, testosterone, prolactin, estradiol, progesterone, fertility alpha-2 microglobulin) in the blood serum and the level of alpha-2 microglobulin of fertility in the endometrial tissue were determined by the method of enzyme immunoassay. Results . The female patients with reproductive dysfunction revealed changes in the concentration of sex hormones. The estradiol concentration was 0.22 (0.21-0.24) nmol/l in the study group of the patients, and 0.25 (0.24-0.28) nmol/l in the healthy patients (z = -3.37, p = 0.0007). The progesterone concentration was 9.57 (7.60-53.54) nmol/l in the study group of the patients and 5.37 (3.59-26.44) nmol/l in the healthy women (z = 1.78, p = 0.07). The level of alpha-2 microglobulin of fertility did not differ significantly in the patients of both the groups. The level of alpha-2 microglobulin of fertility in the endometrium was 0.87 (0.69-1.38) ng/ml in the female patients of the study group and 1.82 (1.38-5.81) ng/ml in the patients of the control group (z = -2.31, p = 0.02). Conclusion . The obtained results are indicative of the involvement of the endometrium in the development of reproductive dysfunction. The data on the levels of alpha-2 microglobulin of fertility in the endometrial tissue are promising for further study and determination of the boundaries of its normal values. Further research in this area will facilitate the development of a test to predict successful implantation.

scholarly journals Clinical significance of the determination of surfactant proteins A and D in assessing the activity of lung sarcoidosis

2018 ◽  
Vol 90 (3) ◽  
pp. 42-46
Author(s):  
V D Beketov ◽  
M V Lebedeva ◽  
N A Mukhin ◽  
A G Serova ◽  
A B Ponomarev ◽  
...  
Keyword(s):  
Clinical Study ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Oxygen Saturation ◽  
Clinical Significance ◽  
Surfactant Proteins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Individuals

The results of a clinical study showing the importance of surfactant proteins A and D in assessing the activity and progression of idiopathic pulmonary fibrosis and chronic lung sarcoidosis are presented. Aim. To study the clinical significance of SP-A, SP-D in assessing the activity of idiopathic pulmonary fibrosis and sarcoidosis. We examined 81 patients with morphologically confirmed diagnoses of idiopathic pulmonary fibrosis (ILF) and sarcoidosis, a control group of 20 healthy individuals. The MSCT of the thoracic organs of the chest was performed, the diffusivity of the lungs was examined, oxygen saturation was determined. In the serum, the surfactant proteins SP-A and SP-D were determined by the enzyme-linked immunosorbent assay. Results. A significant increase in SP-A and SP-D (p

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