Reduced serum BDNF levels are associated with the increased risk for developing MDD: A case-control study with or without antidepressants therapy

Abstract Objective We do not have any consistent markers for major depressive disorder (MDD) though various biological factors are involved in the pathophysiology. We aimed to evaluate the serum brain-derived neurotrophic factor (BDNF) levels in MDD patients with or without antidepressant therapy compared to healthy controls (HCs). Results We assessed serum BDNF levels among three groups: drug-naïve MDD patients (n = 41), drug-treated MDD patients (n = 44), and age-and sex-matched HCs (n = 82). Serum BDNF levels were measured by enzyme-linked immunosorbent assay (ELISA) kit. Serum levels of BDNF were detected significantly lower in drug-naïve MDD patients compared to HCs. No significant alterations of serum BDNF levels between drug-treated patients and HCs were identified. Significant negative correlations between serum BDNF levels and Hamilton depression rating (Ham-D) scores were observed in both drug-naïve and drug-treated MDD patients. Receiver operating characteristic (ROC) analysis showed good diagnostic value for serum BDNF levels in drug-naïve MDD patients with the area under the curve at 0.821. The present study suggests that low serum BDNF levels may be involved in the pathophysiology of MDD. The reduced serum BDNF levels might be used as an early risk assessment marker for major depression.

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Reduced serum BDNF levels are associated with the increased risk for developing MDD: A case-control study with or without antidepressants therapy

10.21203/rs.2.23297/v1 ◽

2020 ◽

Author(s):

Md. Prova Zaman Emon ◽

Rajesh Das ◽

Nuruna Lovely Nishuty ◽

M.M.A. Shalahuddin Qusar ◽

Mohiuddin Ahmed Bhuiyan ◽

...

Keyword(s):

Roc Analysis ◽

Operating Characteristic ◽

Area Under The Curve ◽

Serum Levels ◽

Diagnostic Value ◽

Enzyme Linked Immunosorbent Assay ◽

Increased Risk ◽

Drug Naïve ◽

Control Study ◽

Serum Bdnf

Abstract ObjectiveWe do not have any consistent markers for major depressive disorder (MDD) though various biological factors are involved in the pathophysiology. We aimed to evaluate the serum brain-derived neurotrophic factor (BDNF) levels in MDD patients with or without antidepressant therapy compared to healthy controls (HCs).ResultsWe assessed serum BDNF levels among three groups: drug-naïve MDD patients (n = 41), drug-treated MDD patients (n = 44), and age-and sex-matched HCs (n = 82). Serum BDNF levels were measured by enzyme-linked immunosorbent assay (ELISA) kit. Serum levels of BDNF were detected significantly lower in drug-naïve MDD patients compared to HCs. No significant alterations of serum BDNF levels between drug-treated patients and HCs were identified. Significant negative correlations between serum BDNF levels and Hamilton depression rating (Ham-D) scores were observed in both drug-naïve and drug-treated MDD patients. Receiver operating characteristic (ROC) analysis showed good diagnostic value for serum BDNF levels in drug-naïve MDD patients with the area under the curve at 0.821. The present study suggests that low serum BDNF levels may be involved in the pathophysiology of MDD. The reduced serum BDNF levels might be used as an early risk assessment marker for major depression.

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Expression Analysis of Circulating miR-22, miR-122, miR-217 and miR-367 as Promising Biomarkers of Acute Lymphoblastic Leukemia

10.21203/rs.3.rs-1097151/v1 ◽

2021 ◽

Author(s):

Fatemeh hosseinpour-soleimani ◽

Gholamreza Khamisipour ◽

Zahra Derakhshan ◽

Bahram Ahmadi

Keyword(s):

Acute Lymphoblastic Leukemia ◽

Roc Analysis ◽

Operating Characteristic ◽

Lymphoblastic Leukemia ◽

Area Under The Curve ◽

Diagnostic Value ◽

Healthy Individuals ◽

Quantitative Real Time Pcr ◽

Expression Levels

Abstract Background Currently, the role of serum-based biomarkers such as microRNAs in cancer diagnosis has been extensively established. This study aimed to determine expression levels of bioinformatically selected miRNAs and whether they can be used as biomarkers or a new therapeutic target in patients with Acute Lymphoblastic Leukemia (ALL). Materials and Methods The expression levels of serum miR-22, miR-122, miR-217, and miR-367 in 21 ALL patients and 21 healthy controls were measured using quantitative real-time PCR. The receiver operating characteristic (ROC) curve and the associated area under the curve (AUC) was used to assess candidate miRNAs' diagnostic value as a biomarker. Results The results showed that miR-217 was markedly decreased in patients with ALL compared to controls. Moreover, miR-22, miR-122, and miR-367 were found to be upregulated. Furthermore, ROC analysis showed that serum miR-217 and miR-367 could differentiate ALL patients from the healthy individuals, while miR-22 has approximate discriminatory power that requires further investigation. Conclusion Collectively, the results suggested that miR-217 may play a tumor suppressor role in ALL, whereas miR-22, miR-122, and miR-367 could function as an oncogene. Overall, miR-22, miR-217, and miR-367 could be considered possible biomarkers for the early diagnosis of ALL.

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Serum levels of Trimethylamine-N-oxide in patients with ischemic stroke

Bioscience Reports ◽

10.1042/bsr20190515 ◽

2019 ◽

Vol 39 (6) ◽

Cited By ~ 10

Author(s):

Maimaiti Rexidamu ◽

Hongmei Li ◽

Haiyan Jin ◽

Jiankang Huang

Keyword(s):

Ischemic Stroke ◽

Operating Characteristic ◽

Area Under The Curve ◽

Serum Levels ◽

Clinical Severity ◽

Chinese Patients ◽

Stroke Severity ◽

Increased Risk ◽

Median Level ◽

And Gender

Abstract Objective: Accumulating evidence suggests that Trimethylamine-N-oxide (TMAO), a gut microbial metabolite, is implicated in the pathogenesis of many cardiovascular diseases. The aim of the present study was to investigate the serum levels of TMAO in Chinese patients with ischemic stroke. Method: In the present study, 255 consecutive patients with first-ever acute ischemic stroke and 255 age and gender-matched healthy volunteers were included for testing serum TMAO. Stroke severity was determined by the NIH Stroke Scale (NIHSS). The stroke severity was dichotomized as minor (NIHSS ≤ 5) and moderate-to-high clinical severity (NIHSS > 6). Results: The serum levels of TMAO in stroke ranged from 0.5 to 18.3 μM, with a median value of 5.8 (interquartile range (IQR), 3.3–10.0) μM, which was higher than in those controls (3.9; IQR, 2.6–6.1 μM). The median level of TMAO in those patients was significantly lower than in those moderate-to-high stroke patients (4.1 μM [IQR, 2.8–6.2] vs. 9.1 μM [5.1–11.0]; P<0.001). In univariate and multivariable models, the unadjusted risk of moderate-to-high stroke was increased by 31% (odds ratio (OR) = 1.31 [95% confidence interval (CI): 1.21–1.42], P<0.001) and 22% (OR = 1.22; 95% CI = 1.08–1.32; P<0.001), when TMAO was increased each by 1 μM. Based on the receiver operating characteristic (ROC) curve, the optimal cut-off value of serum level of TMAO as an indicator for screening of moderate-to-high stroke was estimated to be 6.6 μM, which yielded a sensitivity of 69.3 % and a specificity of 79.0%, with the area under the curve at 0.750 (95% CI, 0.687–0.812). Conclusions: Higher TMAO levels were associated with increased risk of first ischemic stroke and worse neurological deficit in Chinese patients.

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Serum miR-101-3p combined with pepsinogen contributes to the early diagnosis of gastric cancer

10.21203/rs.2.15184/v3 ◽

2020 ◽

Author(s):

Weiwei Zeng ◽

Shuxiang Zhang ◽

Lei Yang ◽

Wenchao Wei ◽

Jie Gao ◽

...

Keyword(s):

Gastric Cancer ◽

Early Diagnosis ◽

Roc Analysis ◽

Operating Characteristic ◽

Diagnostic Marker ◽

Diagnostic Value ◽

Serum Markers ◽

Enzyme Linked Immunosorbent Assay ◽

Submucosal Infiltration ◽

Normal Controls

Abstract Background: This study aimed to explore the diagnostic value of serum miR-101-3p combined with pepsinogen (PG) on early diagnosis of gastric cancer (GC). Methods: A total of 61 atrophic gastritis (AG) and 86 GC patients, and 50 healthy volunteers were enrolled. The serum expression of miR-101-3p was measured by qRT-PCR. The serum content of carcinoembryonic antigen (CEA) was measured by Electrochemiluminescence immunoassay. The serum contents of PGI and PGII were measured by Enzyme linked immunosorbent assay. The diagnostic value of serum markers on AG and GC was analyzed by receiver operating characteristic (ROC) analysis. Results: The expression of miR-101-3p, the content of PGI and the ratio of PGI/II were significantly decreased, and the content of PGII was significantly increased in AG patients compared with those in normal controls. The changes of the above serum indicators were more obvious in GC patients than those in AG patients. The content of CEA was significantly higher in GC patients than that in AG patients. In addition, the expression of miR-101-3p was negatively associated with the submucosal infiltration in GC patients. MiR-101-3p exhibited high diagnostic value on AG (AUC 0.8493, sensitivity 80.33%, specificity 80%) and GC (AUC 0.8749, sensitivity 72.09%, specificity 86.49%). MiR-101-3p + PGI + PGI/II (AUC 0.856, sensitivity 80.23%, specificity 77.05%) exhibited a high diagnostic value in distinguishing between AG and GC. Conclusions: MiR-101-3p was a potential diagnostic marker for AG and GC. MiR-101-3p + PGI + PGI/II was effective in distinguishing between AG and GC.

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Dynamics of soluble syndecan-1 in maternal serum during and after pregnancies complicated by preeclampsia: a nested case control study

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2019-0686 ◽

2019 ◽

Vol 58 (1) ◽

pp. 50-58 ◽

Cited By ~ 2

Author(s):

Lorenz Kuessel ◽

Heinrich Husslein ◽

Eliana Montanari ◽

Michael Kundi ◽

Gottfried Himmler ◽

...

Keyword(s):

Endothelial Dysfunction ◽

Predictive Power ◽

Operating Characteristic ◽

Area Under The Curve ◽

Roc Curves ◽

Serum Levels ◽

Maternal Serum ◽

Nested Case Control ◽

Single Time Point ◽

Control Study

Abstract Background We investigated the dynamics and the predictive value of soluble syndecan-1 (Sdc-1), a biomarker of endothelial dysfunction, in uneventful pregnancies and pregnancies complicated by preeclampsia (PE). Methods Serum levels of Sdc-1 were measured at sequential time points during and after uneventful pregnancies (control, n = 95) and pregnancies developing PE (PE_long, n = 12). Levels were further measured in women with symptomatic PE (PE_state, n = 46) at a single time point. Results Sdc-1 levels increased consistently throughout pregnancy. In the PE_long group Sdc-1 levels were lower at all visits throughout pregnancy, and reached significance in weeks 18–22 (p = 0.019), 23–27 (p = 0.009), 28–32 (p = 0.006) and 33–36 (p = 0.008). After delivery, Sdc-1 levels dropped sharply in all pregnancies but were significantly elevated in the PE_long group. The predictive power of Sdc-1 was evaluated analyzing receiver operating characteristic (ROC) curves. A significant power was reached at weeks 14–17 (area under the curve [AUC] 0.65, p = 0.025), 23–27 (AUC 0.73, p = 0.004) and 33–36 (AUC 0.75, p = 0.013). Conclusions In summary, Sdc-1 levels were lower in women developing PE compared to uneventful pregnancies and Sdc-1 might be useful to predict PE. After delivery, Sdc-1 levels remained higher in women with PE. Additional studies investigating the link between glycocalyx degradation, Sdc-1 levels and placental and endothelial dysfunction in pregnancies affected by PE are warranted.

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Evaluation of Serum GDF15, AFP, and PIVKA-II as Diagnostic Markers for HBV-Associated Hepatocellular Carcinoma

Laboratory Medicine ◽

10.1093/labmed/lmaa089 ◽

2020 ◽

Author(s):

Juanjuan Chen ◽

Dongling Tang ◽

Chu Xu ◽

Zhili Niu ◽

Huan Li ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Hepatitis B ◽

Diagnostic Accuracy ◽

Roc Curve ◽

Operating Characteristic ◽

Area Under The Curve ◽

Serum Levels ◽

Diagnostic Value ◽

Serum Marker ◽

B Virus

Abstract Objective To evaluate the potential diagnostic value of growth differentiation factor 15 (GDF15) alone and its combination with protein induced by vitamin K absence-II (PIVKA-II) and alpha-fetoprotein (AFP) for hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC). Methods Serum levels of GDF15, PIVKA-II, and AFP were measured in 110 patients with HBV-associated HCC, 70 patients with HBV-related liver cirrhosis (LC), 70 patients with chronic hepatitis B (CHB), and 110 healthy patients. Results Serum GDF15 was positively related to the levels of PIVKA-II and AFP in patients with HCC (r = 0.352 and r = 0.378; all P <.0001). When the receiver operating characteristic (ROC) curve was plotted for patients with HCC vs all control patients, serum GDF15 had diagnostic parameters of an area under the curve (AUC) of 0.693, a sensitivity of 67.30%, and a specificity of 66.70%, which were lower than parameters for PIVKA-II and AFP (all P <.0001). When the ROC curve was plotted for patients with HCC vs patients with LC, the combination of GDF15 and PIVKA-II had the highest diagnostic accuracy of AUC and specificity as compared with other combinations (all P <.0001). Conclusion We found that GDF15 is a potent serum marker for the detection of HBV-associated HCC and that PIVKA-II combined with GDF15 can improve diagnostic accuracy for HBV-associated HCC.

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Serum MALAT1 Assumes Signifying Capacity in Gastric Cancer Diagnosis.

10.21203/rs.3.rs-76087/v1 ◽

2020 ◽

Author(s):

Keqian Zhang ◽

Tianqi Mao ◽

Zhicheng He ◽

Xiaojiao Wu ◽

Yu Peng ◽

...

Keyword(s):

Gastric Cancer ◽

Roc Analysis ◽

Operating Characteristic ◽

Area Under The Curve ◽

Diagnostic Value ◽

Chain Reaction ◽

Serum Samples ◽

Chi Square ◽

Chi Square Test ◽

Polymerase Chain

Abstract Background: Gastric cancer (GC) represents one of the most serious cancers worldwide with the increasing mortality. Metastasis associated lung adenocarcinoma transcript 1 (MALAT1), a kind of lncRNAs, has been reported to be involved in the progression of cancers. This study aimed to assess serum expression pattern of MALAT1 and its clinical significance in diagnosis of GC.Methods: Serum specimens were collected from 120 GC patients and 58 healthy individuals. The expression profile of MALAT1 was examined using quantitative real-time polymerase chain reaction (qRT-PCR), and its association with clinical parameters was estimated by chi-square test. The diagnostic value of MALAT1 in GC was evaluated by the receiver operating characteristic (ROC) analysis.Results: Upregulated expression of MALTA1 was found in GC patients compared with the healthy controls (P<0.05). The overexpression of MALAT1 was positively correlated with lymph node metastasis (P=0.041) and TNM stage (P=0.005). An area under the curve (AUC) was 0.897 in ROC analysis, suggesting the high diagnostic value of MALAT1. Conclusion: The expression of MALAT1 was upregulated in GC serum samples, and its expression might serve as a potential diagnostic biomarker in patients with GC.

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Diagnostic values of MMP-7, MMP-9, MMP-11, TIMP-1, TIMP-2, CEA, and CA19-9 in patients with colorectal cancer

Journal of International Medical Research ◽

10.1177/03000605211012570 ◽

2021 ◽

Vol 49 (5) ◽

pp. 030006052110125

Author(s):

Xiwen Huang ◽

Yongquan Lan ◽

En Li ◽

Jiaquan Li ◽

Qiaoting Deng ◽

...

Keyword(s):

Colorectal Cancer ◽

Area Under The Curve ◽

Serum Levels ◽

Diagnostic Value ◽

Carbohydrate Antigen ◽

Tissue Inhibitor Of Metalloproteinase ◽

Enzyme Linked Immunosorbent Assay ◽

Detection Model ◽

Specificity And Sensitivity ◽

Combined Detection

Objective Colorectal cancer (CRC) is one of the most common and lethal malignancies. The identification of precise and noninvasive biomarkers is urgently needed to aid the early diagnosis and clinical management of CRC. Methods A total of 112 patients with CRC and 115 healthy control subjects were included in this study. Serum levels of matrix metalloproteinase (MMP)-7, MMP-9, MMP-11, tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-2 were analyzed by enzyme-linked immunosorbent assay, and carcinoembryonic antigen (CEA) and carbohydrate antigen (CA)19-9 levels were measured using an automatic immunoassay analyzer. Results MMP-7, MMP-9, MMP-11, TIMP-1, TIMP-2, CEA, and CA19-9 levels were all significantly higher in CRC patients compared with healthy controls. MMP-7, TIMP-1, and CEA levels were also closely related to clinicopathologic features in patients with CRC. The combination of serum CEA, MMP-7, and TIMP-1 significantly improved the diagnostic value compared with any single marker (area under the curve 0.858–0.890). Furthermore, a combined detection model including MMP-7, TIMP-1, and CEA improved both the specificity and sensitivity for detecting CRC. Conclusions The results showed that combined detection of CEA, MMP-7, and TIMP-1 in serum could provide a specific and sensitive biomarker for the diagnosis of CRC.

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Comparison of the Morphological and Biomechanical Characteristics of Keratoconus, Forme Fruste Keratoconus, and Normal Corneas

10.21203/rs.3.rs-124253/v1 ◽

2020 ◽

Author(s):

Li-Li Guo ◽

Lei Tian ◽

Kai Cao ◽

Yu-Xin Li ◽

Na Li ◽

...

Keyword(s):

Roc Analysis ◽

Operating Characteristic ◽

Case Control Study ◽

Area Under The Curve ◽

Roc Curve Analysis ◽

Corneal Surface ◽

Biomechanical Parameters ◽

Low Efficiency ◽

Control Study ◽

Forme Fruste Keratoconus

Abstract Background: To explore the feasibility of corneal morphological and biomechanical parameters for keratoconus and forme fruste keratoconus diagnosis.Methods: This case-control study included a total of 517 eyes from 408 keratoconus patients (KC group), 83 eyes from 83 forme fruste keratoconus patients (FFKC group), and 158 eyes from 158 patients with normal corneas (NL group). All subjects underwent routine ophthalmology examinations. Pentacam and Corneal Visualization Scheimpflug Technology were used to obtain corneal morphological and biomechanical parameters. Differences between the groups were compared using receiver operating characteristic (ROC) curve analysis.Results: Comparison of the NL group with the KC and FFKC groups (P<0.05), and the NL and KC groups alone (P<0.017), revealed statistically significant differences in corneal morphological and biomechanical parameters, except time from the start until the highest concavity (HCT), deflection length of the first applanation (A1 DfL), and deflection length of the second applanation (A2 DfL). Comparison of the NL and FFKC groups revealed 12 significantly different parameters (P<0.017). ROC analysis showed that all corneal morphological parameters and most biomechanical parameters distinguished KC from NL, with an area under the curve (AUC) greater than 0.80, of which Belin-Ambrósio enhanced ectasia total deviation index (BAD-D) and tomographic and biomechanical index (TBI) were most efficient. Except for central astigmatism from the anterior corneal surface (AstigF), the AUC that distinguished FFKC from NL was 0.862. Other parameters distinguished FFKC from NL with low efficiency. Conclusion: All corneal morphological and most biomechanical parameters are different in KC versus NL, but only a few parameters are different in FFKC versus NL. BAD-D and TBI have the highest efficiency, sensitivity, and specificity for distinguishing KC from NL. The parameters had a low ability to distinguish FFKC from NL. The application of biomechanical instruments to diagnose early keratoconus needs further study.

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Elevated serum levels of malondialdehyde and cortisol are associated with major depressive disorder: A case-control study

SAGE Open Medicine ◽

10.1177/2050312118773953 ◽

2018 ◽

Vol 6 ◽

pp. 205031211877395 ◽

Cited By ~ 16

Author(s):

Md Rabiul Islam ◽

Md Reazul Islam ◽

Imtiaz Ahmed ◽

Abdullah Al Moktadir ◽

Zabun Nahar ◽

...

Keyword(s):

Major Depressive Disorder ◽

Depressive Disorder ◽

Elevated Serum ◽

Area Under The Curve ◽

Serum Levels ◽

Significant Negative Correlation ◽

Diagnostic Value ◽

Enzyme Linked Immunosorbent Assay ◽

Characteristic Analysis ◽

Major Depressive

Objectives: Major depressive disorder is diagnosed on the basis of patient’s self-reported experiences, behavior reported by relatives, and a mental status examination, and yet we do not have any reliable biomarker for this. Mood-regulating pathways are affected by oxidative injury to lipids and cortisol is released into the blood due to stimulation of corticotrophin receptors in the adrenal cortex. Here, we aimed to determine serum levels of malondialdehyde and cortisol in major depressive disorder patients and controls. Methods: We collected blood samples from 247 major depressive disorder patients and 248 controls. Serum levels of malondialdehyde and cortisol were measured by ultraviolet spectrophotometry and enzyme-linked immunosorbent assay kit, respectively. Results: We found malondialdehyde levels were significantly higher in patients than controls, with mean ± standard deviation at 4.49 ± 1.37 and 2.87 ± 0.82 µmol/L, respectively, p < 0.001. Cortisol levels were also found significantly higher in patients than controls, with mean ± SD at 19.22 ± 1.64 and 17.37 ± 1.34 µg/dL, respectively, p < 0.001. Significant negative correlation was observed between serum levels of malondialdehyde and cortisol in patients ( r =−0.170, p = 0.021). Receiver operating characteristic analysis showed good diagnostic value for malondialdehyde and cortisol, with the area under the curve at 0.853 and 0.819, respectively. Conclusion: The present study suggests that increased serum levels of malondialdehyde and cortisol are strongly associated with major depressive disorder. We believe elevations of malondialdehyde and cortisol in serum level arise independently and they could serve as biomarkers for major depressive disorder.

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