Differences of Total Activity and Heat Tolerance of Complement in Plasma Between Black and Yellow Populations

2019 ◽  
Author(s):  
Abdelhakam G. Tamomh ◽  
Xiaojun Jin ◽  
Hui Liu
Keyword(s):  
Heat Tolerance ◽  
Total Activity ◽  
Ratio Method ◽  
Healthy Individuals ◽  
Blood Samples ◽  
Complement Activity ◽  
Temperature Calculation ◽  
Total Complement ◽  
Standard Reaction ◽  
Tolerance Distribution

Abstract Background The goal of this paper is to compare and evaluate the differences in total activity and Heat Tolerance of complement distribution Between Black and Yellow populations.Methods Blood samples of Black and Yellow healthy individuals were randomly collected, and plasma was obtained. The test plasma was diluted in a five series concentration, following with standard reaction and experimental reaction measurements. The basic principle of heat tolerance temperature calculation is to consider the ratio value (ratio method) in which heat tolerance of TCA calculated and plotted according to the area of trapezoidal and triangle as follows: C x°C=OD1-OD5+(OD2-OD5+OD3-OD5+OD4-OD5)*2. Where Cx°C is the total complement activity of the specimen measured in the two reaction conditions.Results The total activity of complement in Black individuals was statistically significantly lower than Yellow individuals (P< 0.05). The heat tolerance of total complement activity was higher among blacks than yellow individuals with no differences between the two groups (P> 0.05). The frequency values of total activity of complement distributed in the yellow individuals was statistically higher than Blacks (P<0.05) and The heat tolerance of total complement activity was statistically higher significant among Blacks than Yellow individuals (P<0.05).Conclusions There is a high differences in total complement activity and heat Tolerance distribution between Black and Yellow populations, may predict health status and innate immunity level differences between African race and Asian race.

scholarly journals A Flow Cytometry-Based Assay for the Measurement of Total Complement Activity in the Serum and Clinical Practice

2022 ◽  
Vol 2022 ◽  
pp. 1-12
Author(s):  
Xuewei Ding ◽  
Shijun Li ◽  
Hui Liu
Keyword(s):  
Flow Cytometry ◽  
Nephrotic Syndrome ◽  
Linear Relationship ◽  
High Volume ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Complement Activity ◽  
Significant Difference ◽  
Cell Groups ◽  
Total Complement

Objective. To develop a novel sensitive and accurate assay suitable for high-volume testing of the total complement activity in the serum for clinical laboratories. Methods. The total complement activity (TCA) to be measured was quantified by detecting the number of fragments produced by erythrocyte lysis and the erythrocyte fragmentation index (EFI), indicating TCA. EFI = M × M 2 / M 1 + M 2 , where M is the number of erythrocyte fragments (removed from the background), M 1 is the number of unagglutinated red cells, M 2 is the number of agglutinated red cell groups, and M 2 / M 1 + M 2 is the agglutination coefficient indicating the degree of erythrocyte agglutination. Mild changes in hemolysin and erythrocyte concentrations were made to optimize the testing conditions. The same serum samples were tested for 10 consecutive days to determine the stability of the experimental results. Serum EFI was detected in both nephrotic syndrome patients and healthy subjects. Results. There was a linear relationship between hemolysin and erythrocyte agglutination ( r = 0.999 , P < 0.001 ). A good linear relationship existed between EFI and TCA ( r = 0.991 , P < 0.001 ). The results were not affected by slight fluctuations in the concentrations of hemolysin or erythrocytes. The interbatch CV = 8.6 % of the test results showed good stability. There was a significant difference in the EFI between nephrotic syndrome patients and healthy individuals, P < 0.001 , and EFI was reduced in nephrotic syndrome patients compared to healthy individuals. Conclusion. The flow cytometry-based assay for TCA was sensitive and accurate and had potential value for clinical application.

scholarly journals Serum Protein Profile Of Iraqi Hydatidosis Patients with Different Sites of Infection

2007 ◽  
Vol 4 (4) ◽  
pp. 612-616
Author(s):  
Baghdad Science Journal
Keyword(s):  
Serum Protein ◽  
Protein Profile ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Healthy Individuals ◽  
Banding Patterns ◽  
Blood Samples ◽  
Molecular Weights ◽  
Serum Protein Profile

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

Computer-Assisted Kinetic Assay for Quantification of Total Complement Activity

1991 ◽  
Vol 8 (2) ◽  
pp. 92-103 ◽  
Author(s):  
M. Abbal ◽  
J. Tkaczuk ◽  
C. Praud ◽  
F. Msayeh ◽  
E. Ohayon
Keyword(s):  
Computer Assisted ◽  
Kinetic Assay ◽  
Complement Activity ◽  
Total Complement

Determination of rate of cross circulation in parabiotic rats with P32-labeled erythrocytes

1959 ◽  
Vol 196 (4) ◽  
pp. 753-756 ◽  
Author(s):  
David G. Fleming ◽  
Laura Caldwell ◽  
Roberta Jacobs
Keyword(s):  
Body Weight ◽  
Blood Volume ◽  
Sodium Phosphate ◽  
Femoral Vein ◽  
Total Activity ◽  
Female Rats ◽  
Blood Samples ◽  
Litter Mate ◽  
Blood Volumes

Litter-mate female rats parabiosed at 21 days by the Bunster-Meyer method were allowed to mature for several months. Volumes of blood obtained from donor animals were incubated at 37°C with P32, in the form of buffered isotonic sodium phosphate. A plasma-free suspension of labeled erythrocytes was prepared and a sample of known activity was injected into the femoral vein of one member of each parabiotic pair. Four pairs of 100 lambda blood samples, obtained by venipuncture, were taken at varying intervals, for the succeeding 150 minutes. Using the dye dilution principle, it was possible to determine the blood volume of the injected rat after the first sample, and that of the pair at the time of equilibrium. The average blood volume was 6.53% of body weight. The concentration of tagged cells reached equal values in both members of the pairs at an average time of 90 minutes. There was less than a 20% loss of total activity in all the pairs used for determinations. An equation was derived for the calculation of the rate of exchange. The average for 15 pairs was 2.09 blood volumes per hour. The range was from 3.95 bl. vol/hr. in the fastest pair to 0.74 bl. vol/hr. in the slowest.

scholarly journals N-Acetylcysteine in Handgrip Exercise: Plasma Thiols and Adverse Reactions

2011 ◽  
Vol 21 (2) ◽  
pp. 146-154 ◽  
Author(s):  
Leonardo F. Ferreira ◽  
Kenneth S. Campbell ◽  
Michael B. Reid
Keyword(s):  
Adverse Reactions ◽  
Venous Blood ◽  
Antioxidant Properties ◽  
Healthy Individuals ◽  
Handgrip Exercise ◽  
Blood Samples ◽  
Glutathione Status ◽  
Plasma Glutathione ◽  
Potential Use ◽  
Plasma Thiols

N-acetylcysteine (NAC) is a thiol donor with antioxidant properties that has potential use as an ergogenic aid. However, NAC is associated with adverse reactions that limit its use in humans.Purpose:The authors evaluated NAC efficacy as a thiol donor before handgrip exercise, measuring changes in serum cysteine and glutathione status and recording adverse reactions in adult subjects across a range of doses.Methods:Healthy individuals ingested NAC capsules (9 ± 2 or 18 ± 4 mg/kg) or solution (0, 35, 70, or 140 mg/kg). Venous blood samples were collected and subjects answered a questionnaire about adverse reactions.Results:Low doses of NAC (capsules) did not affect plasma cysteine or glutathione or cause adverse reactions. Adverse reactions to NAC solution were predominantly mild and gastrointestinal (GI). Intensity of GI reactions to 140 mg/kg NAC was significantly higher than placebo (in a.u., 0.67 ± 0.16 vs. 0.07 ± 0.04; p < .05). Plasma cysteine concentration increased with NAC dose from 9.3 ± 0.7 μM (placebo) to 65.3 ± 6.7 μM (140 mg/kg); however, there was no difference (p > .05) in plasma cysteine for 70 mg/kg vs. 140 mg/kg. Similar increases were observed for the ratio of cysteine to total cysteine, which was directly related to handgrip exercise performance. Plasma glutathione was elevated and oxidized glutathione diminished (p < .05) with NAC 140 mg/kg vs. placebo.Conclusion:NAC effects on plasma thiols are maximized by oral administration of 70 mg/kg, a dose that does not cause significant adverse reactions.

scholarly journals Transmission ofNeisseria meningitidisamong asymptomatic military recruits and antibody analysis

1992 ◽  
Vol 109 (2) ◽  
pp. 241-253 ◽  
Author(s):  
D. A. Caugant ◽  
E. A. Høiby ◽  
E. Rosenqvist ◽  
L. O. Frøholm ◽  
R. K. Selander
Keyword(s):  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Healthy Individuals ◽  
Blood Samples ◽  
Military Recruits ◽  
Electrophoretic Mobilities ◽  
Military Camp ◽  
Close Proximity ◽  
Disease Episode ◽  
Serological Studies

SUMMARYFollowing the occurrence of a case of systemic meningococcal disease in a military camp in Norway, throat cultures and blood samples were collected from 33 healthy individuals belonging to the same troop as the patient (troop A) and from 29 individuals from a different troop (troop B) in the same camp. Serological studies showed that 91% of the recruits had bactericidal antibodies against the disease-causing strain. The isolates ofNeisseria meningitidisrecovered from the throat cultures were serogrouped, serotyped, and assigned to a clone on the basis of an analysis of the electrophoretic mobilities of 14 metabolic enzymes. None of the 23 carriers in troop A harboured the clone responsible for the case of disease, but 6 carried isolates of the same electrophoretic type, ET-7, which was not identified in any of the 19 carriers of troop B. Individuals in troop A were resampled 2 and 17 weeks after the meningococcal disease episode. Five of the carriers had acquired different clones and one of them changed clone twice in that period. Four of the six newly acquired clones had previously been identified in other carriers of troop A, demonstrating transmission of clones among individuals living and working in close proximity.

scholarly journals Anticoagulant Activity of Some Ficus carica Varieties Extracts Grown in Algeria

2019 ◽  
Vol 6 (1) ◽  
pp. 73-78
Author(s):  
Hakima Belattar ◽  
S. Himour
Keyword(s):  
Blood Loss ◽  
Anticoagulant Activity ◽  
Healthy Individuals ◽  
Blood Samples ◽  
Coagulation Assays ◽  
Hemostatic System ◽  
Polyphenolic Extract ◽  
Blood Clots ◽  
Abnormal Bleeding

Abstract The hemostatic system is a complex balanced system that triggers clot formation to prevent blood loss after trauma. To block abnormal bleeding and maintain intravascular blood in a liquid state, in this study we investigated the possible anticoagulant effect of leaves and fruits extracts from some fig varieties grown in Algeria by determining the quick time (QT). The blood samples of the healthy individuals were used. For in vitro coagulation assays, the clotting times obtained in the presence of polyphenols in different extracts of F.carica samples indicate that they exert a high anticoagulant activity on the exogenous pathway of coagulation. Moreover, the polyphenolic extract of ‘Roudane’ variety presented a substantial increase in coagulation. Based on these preliminary results, it can be suggested that the fig polyphenolic extracts (FPE) of this varieties have anticoagulant activity that could be useful in preventing blood clots.

scholarly journals THE DEVELOPMENT OF TOTAL COMPLEMENT ACTIVITY ASSAY BASED ON COMPLEMENT-DEPENDENT IMMUNE LIPOSOME LYSIS

2015 ◽  
Vol 69 (3-4) ◽  
pp. 24-30
Author(s):  
S. N. Skopinskaya ◽  
S. P. Yarkov ◽  
E. N. Khramov ◽  
A. V. Antashev
Keyword(s):  
Blood Serum ◽  
Activity Assay ◽  
Animal Blood ◽  
Complement Activity ◽  
Sheep Erythrocytes ◽  
Wide Range ◽  
Total Complement ◽  
Sulforhodamine 101 ◽  
Internal Volume ◽  
Definition Of

Background: The purpose of work was development of a fast and reproduced procedure for measurement of the total complement activity (ТСА) in human or animal blood serum. Materials and methods: Steady at storage liposomes preparations, which surface sensitized 2,4-DNP haptens, and the internal volume contains calceine or sulforhodamine 101 are obtained. Complement-dependent immune lysis of liposomes at presence of the anti-2,4-DNP immunoglobulines and complement preparations from animals are investigated. Results: It is shown that the degree of liposomes immune lysis depends on complement concentration in a wide range that can be used for definition of TCA level. Research of blood sera from patients has revealed correlation (r =0,793) between data received with the help of liposome immunolytic systems, and the data of nephelometric analysis with application of suspension sheep erythrocytes. Conclusion: The method allows to define total complement activity in blood serum in 15 minutes without separation of reaction components. This might be useful for measurement ТСА level at patients with various diseases and realization of scientific researches.

scholarly journals Study of hemolytic activity and subfactors of complement in smokers intoxication

2019 ◽  
Author(s):  
Rojan Ghanim Al Allaf
Keyword(s):  
Hemolytic Activity ◽  
Normal Values ◽  
Control Group ◽  
Healthy Individuals ◽  
Complement Components ◽  
Complement Activity ◽  
Normal People ◽  
Hemolytic Complement Activity ◽  
Heavy Smokers

Abstract Heavy Smokers appeared to be less resistant to infection, such as bacteria, viruses, and parasites. Many studies have examined the complement components concentrations than compared with normal people and ignored the functional sequencing of complement components, our study included the determination of complement activity by using Sheep red blood cells (SRBCs) as antigen and extracting the hemolytic activity (50%) of complement compounds, and because of difficulty of this method we using statistical analysis program (SPSS 23) and derived the inverse equation which gives the decomposition percentage (1-100%) of complement components by using five serum dilutions only. The total hemolytic complement activity (CH50) and its C3 and C4 fractions were determined in 30 heavy smokers. The results were compared with a control group that contained 30 persons matched in age and sex. Generally, both C3 and C4 concentrations were increased in smoker's individual in compared with the control group. However, when the independent t-test has applied the differences in the C3 and C4 levels in the control group (healthy individuals) and in the smoker group were found to be statistically insignificant but the inverse equation showed a 7% reduction in CH50 in smokers compared with the control group, where 18% reduction was observed. Our current study suggests that the complement components of the heavy smokers suffer from a significant dysfunction in the function, although the concentration of the basic components in the serum is parallel with normal values.

scholarly journals Electrolyte Concentrations in Apparently Healthy Individuals after Consumption of Aqueous Extract of Jatropha tanjorensis

2018 ◽  
pp. 1-7
Author(s):  
Ibiene Sarah Kalio ◽  
Okorie Akuegbo Ezinne
Keyword(s):  
Aqueous Extract ◽  
The Body ◽  
Electrolyte Imbalance ◽  
Herbaceous Plant ◽  
Healthy Individuals ◽  
Selective Electrode ◽  
Blood Samples ◽  
Treatment Of Diabetes ◽  
Control Samples ◽  
Apparently Healthy

Jatropha tanjorensis is a herbaceous plant used locally for the treatment of diabetes, inflammation, and stomach ache in Nigeria. Longtime effect of Jatropha tanjorensis on electrolyte concentrations in the body is yet to be determined. This study was conducted to determine electrolyte concentrations in healthy individuals after consumption of aqueous extract of Jatropha tanjorensis. Seven (7) healthy individuals aged between 23-26 years, weighing 44-75 kg were recruited for the study. Blood samples were collected before the commencement of the study as control samples while test samples were collected on the 7th and 14th day of consumption of 7.8 g/175 ml of Jatropha tanjorensis daily for 14 days. The electrolyte was estimated using an ion selective electrode. The result showed that sodium (p<0.05), potassium (p<0.05), chloride (p<0.05), bicarbonate (p<0.05) and Anion gap (p<0.05) were significantly lower after 7 days of consumption of aqueous extract of Jatropha tanjorensis when compared to results before consumption of the extract. However, after 14 days of consumption of Jatropha tanjorensis aqueous extract, sodium and bicarbonate were lower (p<0.05) when compared to the values before consumption. The results suggest that Jatropha tanjorensis aqueous extract predispose consumers to electrolyte imbalance and metabolic acidosis.

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