scholarly journals Automated Detection and Classification of Ki-67 Stained Nuclear Section Using Machine Learning Based on Texture of Nucleus to Measure Proliferation Score for Prognostic Evaluation of Breast Carcinoma

2020 ◽  
Author(s):  
Anil Kumar ◽  
Manish Prateek
Keyword(s):  
Machine Learning ◽  
Breast Carcinoma ◽  
Automatic Segmentation ◽  
Blue Color ◽  
Ki 67 ◽  
Data Set ◽  
Unsupervised Machine Learning ◽  
Histopathological Images ◽  
Hematoxylin And Eosin

Abstract Background: This study aimed significance of Ki-67 labels and calculated the proliferation score based on the counting of immunopositive and immunonegative nuclear sections with the help of machine learning to predict the intensity of breast carcinoma.Methods: BreCaHAD (Breast Cancer Histopathological Annotation and Diagnosis) dataset includes various malignant cases of different patients in their routine diagnosis. It contains H&E stained microscopic histopathological images at 40x magnification and stored in .tiff format using RGB band. In this study, the method start with preprocessing that focuses on resizing, smoothing and enhancement. After preprocessing, it is decomposed RGB sample into HSI values. BreCaHAD data set is hematoxylin and eosin (H&E) stained, where brown and blue color level have a major role to differentiate the immunopositive and immunonegative nuclear sections. Blue color in RGB and Hue in HSI are the intrinsic characteristic of H&E Ki-67. The shape parameters are calculated after segmentation preceded by Otsu thresholding and unsupervised machine learning. Morphological operators help to solve the problem of overlapping of nucleus section in sample images so that the counting will be correct and increase the accuracy of automatic segmentation.Result: With the help of nine morphological features and supported by unsupervised machine learning technique on BreCaHAD dataset, it is predicted the label of breast carcinoma. The performance measures like precision: 95.7%, recall: 93.8%, f-score: 94.74%, accuracy: 0.9088, specificity: 0.6803, BCR: 0.7975 and MCC: 0.5855 are obtained in proposed methodology which is better than existing techniques. Conclusion: This study developed an efficient automated nuclear section segmentation model implemented on BreCaHAD dataset contains H&E stained microscopic biopsy images. Potentially, this model will assist the pathologist for fast, effective, efficient and accurate computation of Ki-67 proliferation score on breast IHC carcinoma images.

scholarly journals Classification of Daily Irradiance Profiles and the Behaviour of Photovoltaic Plant Elements: The Effects of Cloud Enhancement

2021 ◽  
Vol 11 (11) ◽  
pp. 5230
Author(s):  
Isabel Santiago ◽  
Jorge Luis Esquivel-Martin ◽  
David Trillo-Montero ◽  
Rafael Jesús Real-Calvo ◽  
Víctor Pallarés-López
Keyword(s):  
Machine Learning ◽  
Learning Algorithms ◽  
Automatic Classification ◽  
Sampling Frequency ◽  
Machine Learning Algorithms ◽  
Unsupervised Machine Learning ◽  
Average Efficiency ◽  
Clear Sky ◽  
Photovoltaic Plant

In this work, the automatic classification of daily irradiance profiles registered in a photovoltaic installation located in the south of Spain was carried out for a period of nine years, with a sampling frequency of 5 min, and the subsequent analysis of the operation of the elements of the installation on each type of day was also performed. The classification was based on the total daily irradiance values and the fluctuations of this parameter throughout the day. The irradiance profiles were grouped into nine different categories using unsupervised machine learning algorithms for clustering, implemented in Python. It was found that the behaviour of the modules and the inverter of the installation was influenced by the type of day obtained, such that the latter worked with a better average efficiency on days with higher irradiance and lower fluctuations. However, the modules worked with better average efficiency on days with irradiance fluctuations than on clear sky days. This behaviour of the modules may be due to the presence, on days with passing clouds, of the phenomenon known as cloud enhancement, in which, due to reflections of radiation on the edges of the clouds, irradiance values can be higher at certain moments than those that occur on clear sky days, without passing clouds. This is due to the higher energy generated during these irradiance peaks and to the lower temperatures that the module reaches due to the shaded areas created by the clouds, resulting in a reduction in its temperature losses.

scholarly journals IMAGE BASED RECOGNITION OF DYNAMIC TRAFFIC SITUATIONS BY EVALUATING THE EXTERIOR SURROUNDING AND INTERIOR SPACE OF VEHICLES

2015 ◽  
Vol XL-3/W3 ◽  
pp. 161-168
Author(s):  
A. Hanel ◽  
H. Klöden ◽  
L. Hoegner ◽  
U. Stilla
Keyword(s):  
Machine Learning ◽  
Real Data ◽  
Traffic Situation ◽  
Dynamic Traffic ◽  
Interior Space ◽  
Data Set ◽  
Road Users ◽  
Vehicle Fleet ◽  
New Strategies

Today, cameras mounted in vehicles are used to observe the driver as well as the objects around a vehicle. In this article, an outline of a concept for image based recognition of dynamic traffic situations is shown. A dynamic traffic situation will be described by road users and their intentions. Images will be taken by a vehicle fleet and aggregated on a server. On these images, new strategies for machine learning will be applied iteratively when new data has arrived on the server. The results of the learning process will be models describing the traffic situation and will be transmitted back to the recording vehicles. The recognition will be performed as a standalone function in the vehicles and will use the received models. It can be expected, that this method can make the detection and classification of objects around the vehicles more reliable. In addition, the prediction of their actions for the next seconds should be possible. As one example how this concept is used, a method to recognize the illumination situation of a traffic scene is described. This allows to handle different appearances of objects depending on the illumination of the scene. Different illumination classes will be defined to distinguish different illumination situations. Intensity based features are extracted from the images and used by a classifier to assign an image to an illumination class. This method is being tested for a real data set of daytime and nighttime images. It can be shown, that the illumination class can be classified correctly for more than 80% of the images.

Automatic segmentation and classification of breast masses from mammography using machine learning

2021 ◽  
Author(s):  
Prerna Chander ◽  
Lina Eilouti ◽  
Saubia Khan ◽  
Salam Dhou ◽  
Michel Pasquier ◽  
...  
Keyword(s):  
Machine Learning ◽  
Automatic Segmentation ◽  
Breast Masses

scholarly journals Optical Mapping-Validated Machine Learning Improves Atrial Fibrillation Driver Detection by Multi-Electrode Mapping

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Alexander M. Zolotarev ◽  
Brian J. Hansen ◽  
Ekaterina A. Ivanova ◽  
Katelynn M. Helfrich ◽  
Ning Li ◽  
...  
Keyword(s):  
Machine Learning ◽  
Atrial Fibrillation ◽  
Near Infrared ◽  
Fourier Spectrum ◽  
Optical Mapping ◽  
Binary Classification ◽  
Future Application ◽  
Data Set ◽  
Frequency Spectra

Background: Atrial fibrillation (AF) can be maintained by localized intramural reentrant drivers. However, AF driver detection by clinical surface-only multielectrode mapping (MEM) has relied on subjective interpretation of activation maps. We hypothesized that application of machine learning to electrogram frequency spectra may accurately automate driver detection by MEM and add some objectivity to the interpretation of MEM findings. Methods: Temporally and spatially stable single AF drivers were mapped simultaneously in explanted human atria (n=11) by subsurface near-infrared optical mapping (NIOM; 0.3 mm 2 resolution) and 64-electrode MEM (higher density or lower density with 3 and 9 mm 2 resolution, respectively). Unipolar MEM and NIOM recordings were processed by Fourier transform analysis into 28 407 total Fourier spectra. Thirty-five features for machine learning were extracted from each Fourier spectrum. Results: Targeted driver ablation and NIOM activation maps efficiently defined the center and periphery of AF driver preferential tracks and provided validated annotations for driver versus nondriver electrodes in MEM arrays. Compared with analysis of single electrogram frequency features, averaging the features from each of the 8 neighboring electrodes, significantly improved classification of AF driver electrograms. The classification metrics increased when less strict annotation, including driver periphery electrodes, were added to driver center annotation. Notably, f1-score for the binary classification of higher-density catheter data set was significantly higher than that of lower-density catheter (0.81±0.02 versus 0.66±0.04, P <0.05). The trained algorithm correctly highlighted 86% of driver regions with higher density but only 80% with lower-density MEM arrays (81% for lower-density+higher-density arrays together). Conclusions: The machine learning model pretrained on Fourier spectrum features allows efficient classification of electrograms recordings as AF driver or nondriver compared with the NIOM gold-standard. Future application of NIOM-validated machine learning approach may improve the accuracy of AF driver detection for targeted ablation treatment in patients.

scholarly journals Classification of Brain Tumors from MRI Images Using a Convolutional Neural Network

2020 ◽  
Vol 10 (6) ◽  
pp. 1999 ◽  
Author(s):  
Milica M. Badža ◽  
Marko Č. Barjaktarović
Keyword(s):  
Neural Network ◽  
Machine Learning ◽  
Brain Tumors ◽  
Convolutional Neural Network ◽  
Cross Validation ◽  
Magnetic Resonance Images ◽  
Generalization Capability ◽  
Data Set ◽  
Fold Cross Validation

The classification of brain tumors is performed by biopsy, which is not usually conducted before definitive brain surgery. The improvement of technology and machine learning can help radiologists in tumor diagnostics without invasive measures. A machine-learning algorithm that has achieved substantial results in image segmentation and classification is the convolutional neural network (CNN). We present a new CNN architecture for brain tumor classification of three tumor types. The developed network is simpler than already-existing pre-trained networks, and it was tested on T1-weighted contrast-enhanced magnetic resonance images. The performance of the network was evaluated using four approaches: combinations of two 10-fold cross-validation methods and two databases. The generalization capability of the network was tested with one of the 10-fold methods, subject-wise cross-validation, and the improvement was tested by using an augmented image database. The best result for the 10-fold cross-validation method was obtained for the record-wise cross-validation for the augmented data set, and, in that case, the accuracy was 96.56%. With good generalization capability and good execution speed, the new developed CNN architecture could be used as an effective decision-support tool for radiologists in medical diagnostics.

scholarly journals Bees Assorter

2020 ◽  
Vol 6 (12) ◽  
pp. 61-65
Author(s):  
Himanshu Verma
Keyword(s):  
Machine Learning ◽  
Learning Algorithm ◽  
Machine Learning Algorithms ◽  
Bumble Bee ◽  
Wing Size ◽  
Qualitative And Quantitative Analysis ◽  
Data Set ◽  
Qualitative And Quantitative ◽  
The Difference

Many attempts were made to classify the bees that is bumble bee or honey bee , there have been such a large amount of researches which were made to seek out the difference between them on the premise of various features like wing size , size of bee , color, life cycle and many more. But altogether the analysis there have been either that specialize in qualitative or quantitative , but to beat this issue , thus researchers came up with an answer which might be both qualitative and quantitative analysis made to classify them. And making use of machine learning algorithm to classify them gives a lift . Now the classification would take less time as these algorithms are pretty fast and accurate . By using machine learning work is made easy . Lots of photographs had to be collected and stored for data set. And by using these machine learning algorithms we would be getting information about the bees which might be employed by researchers in further classification of bees. Manipulation of images had to be done so as on prepare them in such a way that they will be applied to the algorithms and have feature extraction done. As there have been a lot of photographs(data set) which take a lot of space and also the area in which bees were present in these photographs were too small so to accommodate it dimension reduction was done , it might not consider other images like trees , leaves , flowers which were there present in the photograph which we elect as a data set.

scholarly journals Image Classification using CNN and Machine Learning

2019 ◽  
pp. 575-580
Author(s):  
G. Keerthi Devipriya ◽  
E. Chandana ◽  
B. Prathyusha ◽  
T. Seshu Chakravarthy
Keyword(s):  
Machine Learning ◽  
Image Classification ◽  
Learning Algorithm ◽  
Machine Learning Algorithm ◽  
Data Set ◽  
Training Models ◽  
Classification Of Images ◽  
Respective Category

Here by in this paper we are interested for classification of Images and Recognition. We expose the performance of training models by using a classifier algorithm and an API that contains set of images where we need to compare the uploaded image with the set of images available in the data set that we have taken. After identifying its respective category the image need to be placed in it. In order to classify images we are using a machine learning algorithm that comparing and placing the images.

scholarly journals A Novel Transcriptomic Classifier for AML Is Highly Associated with Drug Sensitivity

Blood ◽  
2021 ◽  
Vol 138 (Supplement 1) ◽  
pp. 2372-2372
Author(s):  
Habib Hamidi ◽  
Christopher R Bolen ◽  
Elisabeth A Lasater ◽  
Diana Dunshee ◽  
Elizabeth A Punnoose ◽  
...  
Keyword(s):  
Gene Expression ◽  
Machine Learning ◽  
Drug Sensitivity ◽  
Molecular Subtypes ◽  
Ex Vivo ◽  
Response To Therapy ◽  
Publicly Traded ◽  
Data Set ◽  
Unsupervised Machine Learning ◽  
Rnaseq Data

Abstract Introduction: AML is a heterogeneous disease with a wide array of common genetic aberrations. Traditional classification of AML leverages both classical cytogenetics and mutational profiling to stratify patients into four distinct risk groups (ELN). However, tumor gene expression profiles can play an important role in response to therapy, and are potentially useful for unravelling the heterogeneity of AML. In this study, we hypothesized that clinical outcomes and variable responses to therapeutic modalities in AML may be driven by patterns of gene expression, and sought to identify clinically actionable molecular subtypes using the available RNAseq data from the BEAT AML functional genomics study. Methods: Unsupervised machine learning approach based on consensus non-negative matrix factorization (cNMF) was applied to VOOM normalized BEAT-AML RNAseq data from patient samples with ≥50% blasts (N=389) to identify transcriptomic-based molecular subtypes. The subtypes were then compared to the genomic based subtypes for their association with clinical outcome (log-rank test) and ex-vivo drug sensitivity (Kruskal Wallis test). Subtypes were also biologically characterized by gene signature scoring using well curated pathway signatures (GSVA analysis using Hallmark pathways), cell type enrichment (xCell enrichment) and AML differentiation state (scRNAseq signature based on Van Galen et. al). Finally, a random forest classifier was defined based on samples from BEAT AML to predict the NMF subtypes in an independent data set (TCGA AML cohort). Results: Our cNMF based analysis identified six clusters of patients based on the 5,060 (top 10%) most variable genes. These novel subtypes were strongly prognostic (Figure 1A, log rank p=2.79e-08), and were independent of ELN genomic based subtypes (anova p=4.45e-07). Comparison to other genomic based classification is ongoing. The prognostic value of the transcriptomic subtypes was further validated by predicting the subtypes in an independent cohort (TCGA LAML, N=200). We observed a significant association with outcome (Figure 1B, p=0.00013), with clusters 5 and 1 showing markedly better prognosis, similar to BEATAML. These subtypes also displayed unique biological profiles, including significant association with scRNAseq-derived AML differentiation state cell types, Hallmark pathways and cellularity signatures. Notably, clusters 1 and 3 showed a mature phenotype, while clusters 2, 4, and 5 were more progenitor-like (table 1). Importantly, the transcriptomic subtypes were highly predictive of ex-vivo drug sensitivity, with sensitivity to 70 compounds significantly associated with cNMF subtype (Kruskal Wallis p&gt;0.01), compared with 4 in the ELN subtypes.Of the tested molecules, single agent Venetoclax was the most strongly associated with subtype (p=1.7e-13); two subtypes were strongly resistant (median IC50 of 10uM) and four were sensitive, with IC50s in the sub-micromolar range (Table 1). No association was seen between the ELN subtypes and venetoclax sensitivity (p=.35). Conclusions: Unsupervised machine learning-based clustering analysis of transcriptomic data identified six novel subtypes which are similarly prognostic as the ELN genomic based subtype and provide a novel avenue for identifying clinically actionable subsets of AML. Figure 1 Figure 1. Disclosures Hamidi: Genentech: Current Employment, Current equity holder in publicly-traded company. Bolen: Genentech: Current Employment; F. Hoffmann-La Roche: Current equity holder in publicly-traded company. Lasater: Genentech: Current Employment, Current equity holder in publicly-traded company. Dunshee: Genentech/Roche: Current Employment, Current equity holder in publicly-traded company. Punnoose: Genentech: Current Employment, Current equity holder in publicly-traded company. Dail: Genentech/Roche: Current Employment, Current equity holder in publicly-traded company.

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