scholarly journals Bacterial Cellulose Production from Acerola Industrial Waste Using Isolated Kombucha Strain

2021 ◽  
Author(s):  
Eduardo Leonarski ◽  
Karina Cesca ◽  
Sergio Y. G. González ◽  
Débora de Oliveira ◽  
Patrícia Poletto
Keyword(s):  
Bacterial Cellulose ◽  
Industrial Waste ◽  
Biochemical Characterization ◽  
Temperature Stability ◽  
Acetic Acid Bacteria ◽  
Rrna Gene ◽  
High Temperature Stability ◽  
Cellulose Production ◽  
Cultivation Mode ◽  
Static Cultivation

Abstract Bacterial Cellulose (BC) production is still considered expensive and challenging for industries. Herein, BC was produced through an acetic acid bacteria isolated from the kombucha consortium and an extract from acerola juice-industrial waste. The isolated bacterium was characterized through different assays (biochemical characterization and 16S rRNA gene) being identified as Komagataeibacter rhaeticus. BC production with static cultivation mode by the isolated strain was compared using traditional Hestrin-Schramm (HS) medium and acerola waste (AC) (5% w/v). The kinetic behavior of BC production was slightly higher in the HS medium reaching 2.9 g/L after 12 days of fermentation, while 2.3 g/L in the AC medium. Minor differences were observed between crystallite size and d-spacing, highlighting BC produced by the AC medium with higher crystallinity of 93.9% and two-fold breaking stress resistance in comparison with the conventional medium, with high-temperature stability and economically feasible, promissory results for further application of this synthetized cellulose obtained from industrial residues.

scholarly journals Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste

2017 ◽  
Vol 9 (3) ◽  
pp. 387 ◽  
Author(s):  
Nur Arfa Yanti ◽  
Sitti Wirdhana Ahmad ◽  
Sri Ambardini ◽  
Nurhayani Haji Muhiddin ◽  
La Ode Iman Sulaiman
Keyword(s):  
Bacterial Cellulose ◽  
Culture Media ◽  
Liquid Waste ◽  
Acetic Acid Bacteria ◽  
Industrial Sector ◽  
Production Costs ◽  
High Yield ◽  
Cellulose Production ◽  
Bacterial Cellulose Production ◽  
Pineapple Waste

<p class="IsiAbstrakInggris"><span>Bacterial cellulose is a biopolymer </span><span lang="EN-GB">produced by fermentation process with the help of bacteria. It </span><span>has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. </span><span lang="EN-GB">The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was </span><em><span>Acetobacter xylinum</span></em><span lang="EN-GB">. The result of this study showed that </span><em><span>A. xylinum </span></em><span lang="EN-GB">LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production. </span></p>

scholarly journals Bacterial Cellulose: Production, Characterization and Application as Antimicrobial Agent

2021 ◽  
Vol 22 (23) ◽  
pp. 12984
Author(s):  
Dibyajit Lahiri ◽  
Moupriya Nag ◽  
Bandita Dutta ◽  
Ankita Dey ◽  
Tanmay Sarkar ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Therapeutic Potential ◽  
Maximum Yield ◽  
Research Field ◽  
Medical Implants ◽  
Green Composite ◽  
High Moisture ◽  
Cellulose Production ◽  
Pure Cellulose ◽  
Static Cultivation

Bacterial cellulose (BC) is recognized as a multifaceted, versatile biomaterial with abundant applications. Groups of microorganisms such as bacteria are accountable for BC synthesis through static or agitated fermentation processes in the presence of competent media. In comparison to static cultivation, agitated cultivation provides the maximum yield of the BC. A pure cellulose BC can positively interact with hydrophilic or hydrophobic biopolymers while being used in the biomedical domain. From the last two decades, the reinforcement of biopolymer-based biocomposites and its applicability with BC have increased in the research field. The harmony of hydrophobic biopolymers can be reduced due to the high moisture content of BC in comparison to hydrophilic biopolymers. Mechanical properties are the important parameters not only in producing green composite but also in dealing with tissue engineering, medical implants, and biofilm. The wide requisition of BC in medical as well as industrial fields has warranted the scaling up of the production of BC with added economy. This review provides a detailed overview of the production and properties of BC and several parameters affecting the production of BC and its biocomposites, elucidating their antimicrobial and antibiofilm efficacy with an insight to highlight their therapeutic potential.

scholarly journals Bacterial Cellulose Production from Industrial Waste and by-Product Streams

2015 ◽  
Vol 16 (12) ◽  
pp. 14832-14849 ◽  
Author(s):  
Erminda Tsouko ◽  
Constantina Kourmentza ◽  
Dimitrios Ladakis ◽  
Nikolaos Kopsahelis ◽  
Ioanna Mandala ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Industrial Waste ◽  
Cellulose Production ◽  
Bacterial Cellulose Production

UNS N06455

Alloy Digest ◽  
1989 ◽  
Vol 38 (1) ◽  
Keyword(s):  
Corrosion Resistance ◽  
High Temperature ◽  
Stress Corrosion Cracking ◽  
Temperature Stability ◽  
Heat Treating ◽  
High Ductility ◽  
High Temperature Stability ◽  
Nickel Chromium ◽  
Long Time ◽  
Resistance To Stress

Abstract UNS NO6455 is a nickel-chromium-molybdenum alloy with outstanding high-temperature stability as shown by high ductility and corrosion resistance even after long-time aging in the range 1200-1900 F. The alloy also has excellent resistance to stress-corrosion cracking and to oxidizing atmospheres up to 1900 F. This datasheet provides information on composition, physical properties, hardness, elasticity, and tensile properties. It also includes information on corrosion resistance as well as forming, heat treating, machining, and joining. Filing Code: Ni-367. Producer or source: Nickel and nickel alloy producers.

UNS R54620

Alloy Digest ◽  
1987 ◽  
Vol 36 (7) ◽  
Keyword(s):  
Titanium Alloy ◽  
High Temperature ◽  
Time Use ◽  
Temperature Stability ◽  
High Strength ◽  
Heat Treating ◽  
High Temperature Stability ◽  
Beta Titanium Alloy ◽  
Beta Titanium ◽  
Long Time

Abstract UNS No. R54620 is an alpha-beta titanium alloy. It has an excellent combination of tensile strength, creep strength, toughness and high-temperature stability that makes it suitable for service to 1050 F. It is recommended for use where high strength is required. It has outstanding advantages for long-time use at temperatures to 800 F. This datasheet provides information on composition, physical properties, elasticity, tensile properties, and bend strength as well as creep. It also includes information on high temperature performance and corrosion resistance as well as forming, heat treating, machining, joining, and surface treatment. Filing Code: Ti-86. Producer or source: Titanium alloy mills.

Comparative Assessment of Sporophyte and Gametophyte Thermal Resistance of Winter Rape

2019 ◽  
pp. 15-22
Keyword(s):  
Elevated Temperatures ◽  
Rapid Assessment ◽  
Temperature Stability ◽  
Temperature Factor ◽  
Plant Genome ◽  
Limiting Factors ◽  
Initial Assessment ◽  
High Temperature Stability ◽  
Winter Rape ◽  
High Yields

The temperature factor is one of the limiting factors for obtaining high yields of crops, so one of the main tasks of selection is to search for temperature-resistant genotypes and to create on their basis the banks of crops with high temperature stability. The first step to solving this problem is to conduct a rapid assessment of the temperature plasticity of large populations and to isolate breeding-valuable genotypes from them. There are numerous methods that allow, in the short term with minimal technical and material costs, to carry out an initial assessment of a large number of genotypes at sporophytic level and differentiate them by resistance to the temperature factor. These methods include the method of estimating pollen populations. These studies have repeatedly been conducted on many cultures, their correctness is due to the expression of a large part of the plant genome, both at the diploid and haploid levels of development and demonstrated by many studies in this direction. The aim of our study was to study the stability of gametophyte and sporophyte of collecting varieties and varieties of winter rape to elevated temperatures, to study the correlation between the heat resistance of sporophyte and gametophyte.

Screening and Molecular Characterization of Cellulase Producing Actinobacteria from Litchi Orchard

2019 ◽  
Vol 13 (1) ◽  
pp. 90-101
Author(s):  
Sanju Kumari ◽  
Utkarshini Sharma ◽  
Rohit Krishna ◽  
Kanak Sinha ◽  
Santosh Kumar
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Evolutionary Relationship ◽  
Gene Sequencing ◽  
Cellulase Activity ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Rrna Gene Sequencing

Background: Cellulolysis is of considerable economic importance in laundry detergents, textile and pulp and paper industries and in fermentation of biomass into biofuels. Objective: The aim was to screen cellulase producing actinobacteria from the fruit orchard because of its requirement in several chemical reactions. Methods: Strains of actinobacteria were isolated on Sabouraud’s agar medium. Similarities in cultural and biochemical characterization by growing the strains on ISP medium and dissimilarities among them perpetuated to recognise nine groups of actinobacteria. Cellulase activity was measured by the diameter of clear zone around colonies on CMC agar and the amount of reducing sugar liberated from carboxymethyl cellulose in the supernatant of the CMC broth. Further, 16S rRNA gene sequencing and molecular characterization were placed before NCBI for obtaining recognition with accession numbers. Results: Prominent clear zones on spraying Congo Red were found around the cultures of strains of three groups SK703, SK706, SK708 on CMC agar plates. The enzyme assay for carboxymethylcellulase displayed extra cellulase activity in broth: 0.14, 0.82 and 0.66 &#181;mol mL-1 min-1, respectively at optimum conditions of 35°C, pH 7.3 and 96 h of incubation. However, the specific cellulase activities per 1 mg of protein did not differ that way. It was 1.55, 1.71 and 1.83 μmol mL-1 min-1. The growing mycelia possessed short compact chains of 10-20 conidia on aerial branches. These morphological and biochemical characteristics, followed by their verification by Bergey’s Manual, categorically allowed the strains to be placed under actinobacteria. Further, 16S rRNA gene sequencing, molecular characterization and their evolutionary relationship through phylogenetics also confirmed the putative cellulase producing isolates of SK706 and SK708 subgroups to be the strains of Streptomyces. These strains on getting NCBI recognition were christened as Streptomyces glaucescens strain SK91L (KF527284) and Streptomyces rochei strain SK78L (KF515951), respectively. Conclusion: Conclusive evidence on the basis of different parameters established the presence of cellulase producing actinobacteria in the litchi orchard which can convert cellulose into fermentable sugar.

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