scholarly journals Development of Optical Biosensor For The Detection of Glutamine in Human Biofluids Using Merocyanine Dye

2021 ◽  
Author(s):  
Vijayakumar Sathya ◽  
Appadurai Deepa ◽  
Lakshmi Kandhan Sangeetha ◽  
Venkatesan Srinivasadesi ◽  
Shyi-Long Lee ◽  
...  
Keyword(s):  
Organic Compound ◽  
Optical Biosensor ◽  
Linear Range ◽  
Practical Application ◽  
Experimental Conditions ◽  
Fluorescent Intensity ◽  
Neutral Ph

Abstract Merocyanine dye based fluorescent organic compound has been synthesized for the detection of glutamine. The probe showed remarkable fluorescent intensity with glutamine through ICT. Hence, it is tested for the detection of glutamine using colorimetric and fluorimetric techniques in physiological and neutral pH (7.2). Under optimized experimental conditions, the probe detects glutamine selectively among other interfering biomolecules. The probe has showed a LOD of 9.6 nm at the linear range 20-180 µM towards glutamine. The practical application of the probe is successfully tested in human biofluids.

scholarly journals Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2745 ◽  
Author(s):  
Lu Xu ◽  
Jun Liang ◽  
Yonghui Wang ◽  
Shuyue Ren ◽  
Jin Wu ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
Quantitative Detection ◽  
Practical Application ◽  
Full Agreement ◽  
Experimental Conditions ◽  
Dose Time ◽  
Dimethyl Ammonium ◽  
Rice Samples ◽  
Optimized Conditions

A highly selective and sensitive method for Cd(II) detection was developed based on aptamer and gold nanoparticles (AuNPs) combined with a colorimetric smartphone readout. The experimental conditions such as reaction time of polydiene dimethyl ammonium chloride (PDDA) and AuNPs, PDDA dose, time of aptamer and PDDA incubation, and aptamer concentration were optimized. Under the optimized conditions, the color and red(R) value of the solution was concentration-dependent on Cd(II). The proposed method exhibited a linear range of 1–400 ng/mL (r2 = 0.9794) with a limit of detection (LOD) of 1 ng/mL. This method had been successfully applied to test and quantify Cd(II) in water and rice samples, and the results were in full agreement with those from the atomic absorption spectrometer. Therefore, low-cost colorimetry demonstrated its potential for practical application in visual or quantitative detection with a smartphone. This approach can be readily applied to other analytes.

Solar photo-Fenton-like process at neutral pH: Fe(III)-EDDS complex formation and optimization of experimental conditions for degradation of pharmaceuticals

2019 ◽  
Vol 328 ◽  
pp. 259-266 ◽  
Author(s):  
Elisabeth Cuervo Lumbaque ◽  
Débora Salmoria Araújo ◽  
Thágor Moreira Klein ◽  
Elaine R. Lopes Tiburtius ◽  
Jacqueline Argüello ◽  
...  
Keyword(s):  
Complex Formation ◽  
Experimental Conditions ◽  
Neutral Ph ◽  
Optimization Of Experimental Conditions

scholarly journals A Facile Strategy for Immobilizing GOD and HRP onto Pollen Grain and Its Application to Visual Detection of Glucose

2020 ◽  
Vol 21 (24) ◽  
pp. 9529
Author(s):  
Shanxia Jin ◽  
Liping Liu ◽  
Mengying Fan ◽  
Yaru Jia ◽  
Ping Zhou
Keyword(s):  
Visual Detection ◽  
Color Change ◽  
Cost Effective ◽  
Pollen Grain ◽  
Practical Application ◽  
Horse Radish Peroxidase ◽  
Experimental Conditions ◽  
Naked Eye ◽  
Cost Effective Approach ◽  
Affinity Interaction

Pollen grain was explored as a new carrier for enzyme immobilization. After being modified with boric acid-functionalized titania, the pollen grain was able to covalently immobilize glycosylated enzymes by boronate affinity interaction under very mild experimental conditions (e.g., pH 7.0, ambient temperature and free of organic solvent). The glucose oxidase and horse radish peroxidase-immobilized pollen grain became a bienzyme system. The pollen grain also worked as an indicator of the cascade reaction by changing its color. A rapid, simple and cost-effective approach for the visual detection of glucose was then developed. When the glucose concentration exceeded 0.5 mM, the color change was observable by the naked eye. The assay of glucose in body fluid samples exhibited its great potential for practical application.

Removal of cadmium and zinc from contaminated wastewater using Rhodobacter sphaeroides

2016 ◽  
Vol 75 (11) ◽  
pp. 2489-2498 ◽  
Author(s):  
Xiaomin Li ◽  
Weihua Peng ◽  
Yingying Jia ◽  
Lin Lu ◽  
Wenhong Fan
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Removal Rate ◽  
Inoculum Size ◽  
Pilot Test ◽  
Optimal Conditions ◽  
Practical Application ◽  
Experimental Conditions ◽  
Initial Concentration ◽  
Effects Of Ph ◽  
Remediation Time

Rhodobacter sphaeroides was used for bioremediation of wastewater polluted with cadmium (Cd) and zinc (Zn). The tolerance of the microorganism to selected heavy metals (HMs), as well as the effects of pH, temperature and inoculum size on the removal rate, was investigated. The remediation effects of R. sphaeroides were analysed at different initial concentrations of HMs. Bioremediation mechanisms were thoroughly discussed based on the results from the cell characterisation analysis. Cd and Zn could inhibit the growth of R. sphaeroides. However, Cd was more toxic than Zn, with corresponding EC50 values of 5.34 and 69.79 mg L−1. Temperature and pH had greater influence on the removal rate of HMs than inoculum size. The optimal conditions for temperature and pH were 35 °C–40 °C and pH 7, respectively. Initial concentration of HMs and remediation time also affected the removal rate. Rhodobacter sphaeroides had a relatively higher remediation effect under the present experimental conditions. The removal rates for Cd and Zn reached 97.92% and 97.76%, respectively. Results showed that biosorption and HM precipitation were the main bioremediation mechanisms. This information is necessary to better understand the removal mechanism of R. sphaeroides, and is significant for its pilot test and future practical application.

scholarly journals Does the natural carcinogen ptaquiloside degrade readily in groundwater?

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Jane S. Wu ◽  
Frederik Clauson-Kaas ◽  
Dan Nybro Lindqvist ◽  
Lars Holm Rasmussen ◽  
Bjarne W. Strobel ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Drinking Water ◽  
Degradation Kinetics ◽  
Low Ph ◽  
Experimental Conditions ◽  
Neutral Ph ◽  
Rate Of Hydrolysis ◽  
Acidic Conditions ◽  
Ph Conditions ◽  
Ph Range

Abstract Background Ptaquiloside (PTA) is a natural carcinogen found in bracken ferns. PTA is released from the plants via soil to surface and groundwaters from where humans can be exposed via drinking water. Primary degradation of PTA is due to hydrolysis with formation of pterosin B (PTB). Temperature and pH determine the rate of hydrolysis under pure experimental conditions. To assess the applicability of the experimental model to natural groundwaters, PTA degradation kinetics were examined in a range of natural groundwaters at environmentally relevant conditions. Results PTA was quantified by UPLC-MS/MS. Over an 80-day study period, PTA half-lives ranged from 6.5 to 47 days (natural pH; 8.0 °C). The fastest degradation was observed for the most alkaline groundwaters with pH of around 8. Rates of degradation were well predicted using an existing mathematical model for hydrolysis. However, deviations from this model were found, especially at the extremes of the examined pH-range (4.7–8.2). The degree of conversion of PTA to PTB was close to unity around neutral pH. However, at slightly acidic conditions, formation of PTB could only count for 9% of the degraded PTA, indicating formation of other products. Conclusions Degradation of PTA in groundwater is determined by pH and temperature, and PTA can prevail for months under slightly acid to neutral pH conditions. The existing laboratory-based model for PTA hydrolysis is generally applicable for groundwaters but needs further validation at high and low pH.

Facile Synthesis of Ag/AgCl Grafted AgBi(MoO4)2 with Enhanced Photocatalytic Performance Under Visible Light

NANO ◽  
2018 ◽  
Vol 13 (08) ◽  
pp. 1850089 ◽  
Author(s):  
Xu Yan ◽  
Ziyang Wu ◽  
Yong Zhao ◽  
Biao Liu ◽  
Yubin Tang
Keyword(s):  
Visible Light ◽  
Practical Application ◽  
Ag Nps ◽  
Experimental Conditions ◽  
Degradation Ratio ◽  
Activity Promotion ◽  
High Efficient ◽  
Spr Effect ◽  
Insight Into

Silver nanoparticles (NPs) and related semiconductors are a family of very important and widely applied photocatalysts. However, the preparation of high stability and activity of this photocatalyst is still a challenge. In this work, we report a stable Ag/AgCl/AgBi(MoO[Formula: see text] heterojunction photocatalyst fabricated via a simple in situ anion-exchange reaction followed by the photoreduction treatment. In the treatment of tetracycline (TC) wastewater under visible light, the AC-0.4 sample (prepared with 0.4[Formula: see text]mmol KCl) exhibits the significantly improved activity (degradation ratio, DR of 71.3%) compared with the pristine AgBi(MoO[Formula: see text] (30% DR) and Ag/AgCl sample (synthesized by the photoreduction of AgCl) (37% DR) under identical experimental conditions. This activity promotion is from the fast interfacial electron transfer between the heterojunction phases of AgCl/AgBi(MoO[Formula: see text] and the SPR effect of Ag NPs. After five successive recycles, the AC-0.4 sample still maintains good stability and activity for TC degradation, which shows a great potential to be used in practical application. Through the ESR and controlled scavenged experiments, we found the [Formula: see text]OH and [Formula: see text]O[Formula: see text] are the major reactive intermediate species in the TC photodegradation reaction. Our work provides a new insight into the synthesis of stable and high efficient Ag-based heterojunction photocatalysts for the application of wastewater treatment.

Application of Mercaptostyrene Divinyl Benzene Immobilized on Ion-Exchange Polymeric Membrane as Hg(II) Sensor

2020 ◽  
Vol 18 (1) ◽  
pp. 1-4
Author(s):  
Abdellah Hamel ◽  
Karima Morakchi ◽  
Amel Bendjama ◽  
Hiba Mahfoud ◽  
Rochedi Kherrat
Keyword(s):  
Detection Limit ◽  
Impedance Spectroscopy ◽  
Ion Exchange ◽  
Electrochemical Sensor ◽  
Linear Range ◽  
Polymeric Membrane ◽  
Experimental Conditions ◽  
Low Detection Limit ◽  
Divinyl Benzene

Herein, we report the fabrication of electrochemical sensor for the detection of mercury Hg(II) ions using macroporous divinyl benzene immobilized on ion-exchange polymeric ionophore. Impedance spectroscopy was adopted to examine the performance of the fabricated sensor. Various experiments were done to get the optimized experimental conditions of the fabricated sensor. The fabricated sensor demonstrates a linear range of 10–8 to 10–5 M and low detection limit was about 10–4.8 M.

scholarly journals Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1265 ◽  
Author(s):  
Qingkui Fang ◽  
Quan Zu ◽  
Xiude Hua ◽  
Pei Lv ◽  
Wanwen Lin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Linear Range ◽  
Enzyme Linked Immunosorbent Assay ◽  
Experimental Conditions ◽  
Coating Antigen ◽  
Relative Standard

A sensitive biotinylated indirect competitive enzyme-linked immunosorbent assay (Bic-ELISA) was developed to detect acetamiprid pesticides in pollen, based on the heterogeneous coating antigen and biotinylated anti-acetamiprid monoclonal antibody. Under optimized experimental conditions, the detection limit for the Bic-ELISA was 0.17 ng/mL and the linear range was 0.25–25 ng/mL. The cross-reactivities could be regarded as negligible for the biotinylated antibodies with their analogues except for thiacloprid (1.66%). Analyte recoveries for extracts of spiked pollen (camellia pollen, lotus pollen, rape pollen) ranged from 81.1% to 108.0%, with intra-day relative standard deviations (RSDs) of 4.8% to 10.9%, and the average reproducibility was 85.4% to 110.9% with inter-assay and inter-assay RSDs of 6.1% to 11.7%. The results of Bic-ELISA methods for the Taobao’s website samples were largely consistent with HPLC-MS/MS. Therefore, the established Bic-ELISA methods would be conducive to the monitoring of acetamiprid in pollen.

An investigation into the relationship between the metabolism of pyruvate in bull spermatozoa and fertility

1956 ◽  
Vol 48 (2) ◽  
pp. 153-159 ◽  
Author(s):  
G. Glew
Keyword(s):  
Metabolic Response ◽  
Conception Rate ◽  
Low Fertility ◽  
High Fertility ◽  
Practical Application ◽  
Experimental Conditions ◽  
Conception Rates ◽  
The Mean ◽  
Dairy Bulls ◽  
The Relationship

1. Two hundred and ninety-one samples of semen from seventy-four dairy bulls in use at seven artificial insemination centres and one bull-rearing centre were tested and graded according to the type of metabolic response under the experimental conditions described by Melrose & Terner (1953).2. Conception rates of bulls were obtained from the breeding records based on not less than 150 first inseminations. Since the mean conception rates of centres varied, the fertility of each bull was assessed in relation to the mean conception rate of the centre at which the bull was standing. Bulls were considered of ‘high’ fertility when their conception rates were above, and of ‘low’ fertility when their conception rates were below a dividing line 5% below the mean conception rate of the centre.3. A highly significant relationship (P < 0·001) was found between the groups of the metabolic responses and the fertility levels of the bulls. The practical application of the test in the assessment of the potential fertility of individual bulls is discussed.

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