scholarly journals Selective Estrogen Receptor Modulator Lasofoxifene Suppresses Spondyloarthritis Manifestation and Affects Characteristics of Gut Microbiota in Zymosan-induced SKG Mice

2020 ◽  
Author(s):  
Hyemin Jeong ◽  
In Young Kim ◽  
Eun-Kyung Bae ◽  
Chan Hong Jeon ◽  
Kwang-Sung Ahn ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Gut Microbiota ◽  
Small Animal ◽  
Joint Inflammation ◽  
Fecal Calprotectin ◽  
Mineral Density ◽  
Receptor Modulator ◽  
Positron Emission ◽  
Significant Difference ◽  
Microbiota Diversity

Abstract Background: Ankylosing spondylitis is a male-predominant disease and previous study revealed that estrogen has an anti-inflammatory effect on the spondyloarthritis (SpA) manifestations in zymosan-induced SKG mice. This study aimed to evaluate the effect of selective estrogen receptor (ER) modulator lasofoxifene (Laso) on disease activity of SpA. Methods: Mice were randomized into zymosan-treated, zymosan + 17β-estradiol (E2)-treated, and zymosan + Laso-treated groups. Clinical arthritis scores were measured, and 18F-fluorodeoxyglucose (18F-FDG) small-animal positron emission tomography/computed tomography was performed to quantify joint inflammation. Bone mineral density (BMD) was measured. Fecal samples were collected and 16S ribosomal RNA gene sequencing was used to determine gut microbiota differences. Results: Both zymosan + E2-treated mice and zymosan + Laso-treated mice showed lower arthritis clinical scores and lower 18F-FDG uptake than zymosan-treated mice. BMD was significantly higher in zymosan + E2-treated mice and zymosan + Laso-treated mice than zymosan-treated mice, respectively. Fecal calprotectin levels were significantly elevated at 8 weeks after zymosan injection in zymosan-treated mice, but it was not significantly changed in zymosan + E2-treated mice and zymosan + Laso-treated mice. Gut microbiota diversity of zymosan-treated mice was significantly different from zymosan + E2-treated mice and zymosan + Laso-treated mice, respectively. There was no significant difference in gut microbiota diversity between zymosan + E2-treated mice and zymosan + Laso -treated mice. Conclusions: Selective ER modulator Laso inhibited joint inflammation and enhanced BMD in SKG mice, a model of SpA. Laso also affected the composition and biodiversity of gut microbiota. This study provides new knowledge regarding that selected SpA patients could benefit from selective ER modulator treatment.

scholarly journals Selective estrogen receptor modulator lasofoxifene suppresses spondyloarthritis manifestation and affects characteristics of gut microbiota in zymosan-induced SKG mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyemin Jeong ◽  
In Young Kim ◽  
Eun-Kyung Bae ◽  
Chan Hong Jeon ◽  
Kwang-Sung Ahn ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Gut Microbiota ◽  
Small Animal ◽  
Joint Inflammation ◽  
Fecal Calprotectin ◽  
Selective Estrogen Receptor Modulator ◽  
Mineral Density ◽  
Receptor Modulator ◽  
Significant Difference ◽  
Microbiota Diversity

AbstractAnkylosing spondylitis is a male-predominant disease and previous study revealed that estrogens have an anti-inflammatory effect on the spondyloarthritis (SpA) manifestations in zymosan-induced SKG mice. This study aimed to evaluate the effect of selective estrogen receptor modulator (SERM) lasofoxifene (Laso) on disease activity of SpA. Mice were randomized into zymosan-treated, zymosan + 17β-estradiol (E2)-treated, and zymosan + Laso-treated groups. Arthritis was assessed by 18F-fluorodeoxyglucose (18F-FDG) small-animal positron emission tomography/computed tomography and bone mineral density (BMD) was measured. Fecal samples were collected and 16S ribosomal RNA gene sequencing was used to determine gut microbiota differences. Both zymosan + E2-treated mice and zymosan + Laso-treated mice showed lower arthritis clinical scores and lower 18F-FDG uptake than zymosan-treated mice. BMD was significantly higher in zymosan + E2-treated mice and zymosan + Laso-treated mice than zymosan-treated mice, respectively. Fecal calprotectin levels were significantly elevated at 8 weeks after zymosan injection in zymosan-treated mice, but it was not significantly changed in zymosan + E2-treated mice and zymosan + Laso-treated mice. Gut microbiota diversity of zymosan-treated mice was significantly different from zymosan + E2-treated mice and zymosan + Laso-treated mice, respectively. There was no significant difference in gut microbiota diversity between zymosan + E2-treated mice and zymosan + Laso -treated mice. Laso inhibited joint inflammation and enhanced BMD in SKG mice, a model of SpA. Laso also affected the composition and biodiversity of gut microbiota. This study provides new knowledge regarding that selected SpA patients could benefit from SERM treatment.

scholarly journals Diversity in the intestinal microbiota and the influence of delivery, dietary profile and nutritional status

2021 ◽  
Vol 2 (5) ◽  
Author(s):  
Franciele Valduga ◽  
◽  
Malena Rostirola Miri ◽  
Daniela Signori ◽  
Larissa Pereira da Costa ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Gut Microbiota ◽  
School Children ◽  
Alpha Diversity ◽  
Grape Juice ◽  
Anthropometric Parameters ◽  
Culture Methods ◽  
Normal Delivery ◽  
Significant Difference ◽  
Microbiota Diversity

Objective: This research aimed to evaluate the nutritional status and influence on the dietary profile in the gut microbiota, in an attempt to identify possible effects of grape juice consumption among school children aged 6-10. Design: Anthropometric parameters (weight, height, Body Mass Index, waist circumference, triceps, and subscapular skinfold) weremeasured at baseline. A Food Frequency Questionnaire (FFQ) was carried out to evaluate the dietary profile. Feces were analyzed by culture methods and the alpha diversity was determined by the Shannon Index. Participants: 36 volunteer school children aged 6 to 10 years from two different cities in the South of Brazil. Main outcome measure: Gut microbiota diversity according to anthropometric parameters, nutrition profile and delivery patterns. Analysis: For statistical analysis, t or u test and correlation were used through the statistical software SPSS® version 22.0. Results: The volunteers presented a eutrophic nutritional status, but 41.7% in the City 2 were overweight. There was a significant difference in the consumption of polyphenols (p<0.003), microbiotadiversity, breastfeeding, and delivery patterns by city. However, the dietary profile does not include the portions of daily nutrients recommended in both cities. The volunteers from City 1 presented a greater alpha diversity, which may be related to higher micronutrient intake, breastfeeding, and predominant natural/vaginal delivery Conclusion: We observed that the normal delivery and the dietary profile seems to be important factors to the gut microbiota diversity in these children. Keywords: grape juice; dietary profile; gut microbiota.

scholarly journals The Studies to correlation between gut microbiota diversity and functional erectile dysfunction

2021 ◽  
Author(s):  
Qiang Geng ◽  
Shaofeng Chen ◽  
Yuan Sun ◽  
Yu Zhao ◽  
Zhong Li ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
Gut Microbiota ◽  
Alpha Diversity ◽  
T Test ◽  
Data Quality Control ◽  
Sequencing Data ◽  
Significant Difference ◽  
Miseq Platform ◽  
Group 3 ◽  
Microbiota Diversity

Abstract Objective: To analyze the distribution of gut microbiota in the ED patients, and explore the relationship between the diversity of gut microbiota and psychogenic erectile dysfunction. Methods: 30 cases of patients with erectile dysfunction (ED) and 30 healthy persons (healthy donor, HD) stool specimen were collected, using Illumina's Miseq platform samples V3-V4 region sequences bacterial 16SrRNA gene Paired end (PE) 300 sequencing, sequencing results were analyzed differences in species composition and diversity. Analysis contains five modules: sequencing data quality control, OTU species clustering and annotation, alpha diversity, beta diversity and the use of T-test and the analysis of the LEfSe differences. Results: 1. The flora diversity in the group of ED than HD significantly different (P<0.01), ED group has a low bacterial diversity. 2. Between ED group and HD group, abundant bacteria (TOPlO) and core flora (90%) had no significant difference in the genus level; all bacteria flora (>1%) display, Alloprevotella groups genus presents differences, Alloprevotella only be identified in the HD group. 3. ED and HD groups present in well separated PCoA analysis, having a significant difference in the two kinds of microflora. 4.T-test shows six species were significantly different, in the ED group, Streptococcus and Subdoligranulum were increasing, and Prevotella, Prevotella sp.9, Blautia, Lachnospiraceae NK4A136 groups and Roseburia were decreasing. 5.LEfSe analysis revealed 24 species were significantly different between ED and HD groups. Conclusion: Gene sequencing was performed on the two groups of specimens and finding that microbial community structure and diversity had significant difference, suggesting that ED have low gut microbiota diversity.

scholarly journals Characterization of the Gut Microbiota of Individuals at Different T2D Stages Reveals a Complex Relationship with the Host

2020 ◽  
Vol 8 (1) ◽  
pp. 94 ◽  
Author(s):  
Alejandra Chávez-Carbajal ◽  
María Luisa Pizano-Zárate ◽  
Fernando Hernández-Quiroz ◽  
Guillermo Federico Ortiz-Luna ◽  
Rosa María Morales-Hernández ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Metabolic Pathways ◽  
Dietary Intervention ◽  
Metabolic Characteristics ◽  
Significant Difference ◽  
Bacterial Richness ◽  
Microbiota Diversity ◽  
Improve Glucose Tolerance ◽  
M 14

In this work, we studied 217 Mexican subjects divided into six groups with different stages of glucose intolerance: 76 Controls (CO), 54 prediabetes (PRE), 14 T2D no medication (T2D−No−M), 14 T2D with Metformin (T2D−M), 22 T2D with polypharmacy (T2D−P), and 37 T2D with polypharmacy and insulin (T2D−P+I). We aimed to determine differences in the gut microbiota diversity for each condition. At the phylum level, we found that Firmicutes and Bacteroidetes outline major changes in the gut microbiota. The gut bacterial richness and diversity of individuals in the T2D−No−M group were lesser than other groups. Interestingly, we found a significant difference in the beta diversity of the gut microbiota among all groups. Higher abundance was found for Comamonadaceae in PRE, and Sutterella spp. in T2D−No−M. In addition, we found associations of specific microbial taxa with clinical parameters. Finally, we report predicted metabolic pathways of gut microbiota linked to T2D−M and PRE conditions. Collectively, these results indicate that each group has specific predicted metabolic characteristics and gut bacteria populations for each phenotype. The results of this study could be used to define strategies to modulate gut microbiota through noninvasive treatments, such as dietary intervention, probiotics or prebiotics, and to improve glucose tolerance of individuals with prediabetes or T2D.

scholarly journals Colitis‐induced colorectal cancer and intestinal epithelial estrogen receptor beta impact gut microbiota diversity

2019 ◽  
Vol 144 (12) ◽  
pp. 3086-3098 ◽  
Author(s):  
Ahmed Ibrahim ◽  
Luisa W. Hugerth ◽  
Linnea Hases ◽  
Ashish Saxena ◽  
Maike Seifert ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Estrogen Receptor ◽  
Gut Microbiota ◽  
Estrogen Receptor Beta ◽  
Intestinal Epithelial ◽  
Microbiota Diversity ◽  
Receptor Beta

scholarly journals Evidence for selective estrogen receptor modulator activity in a black cohosh (Cimicifuga racemosa) extract: comparison with estradiol-17beta

2003 ◽  
pp. 351-362 ◽  
Author(s):  
D Seidlova-Wuttke ◽  
O Hesse ◽  
H Jarry ◽  
V Christoffel ◽  
B Spengler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Selective Estrogen Receptor Modulator ◽  
Ovariectomized Rats ◽  
Tartrate Resistant Acid Phosphatase ◽  
Complement Protein ◽  
Cimicifuga Racemosa ◽  
Mineral Density ◽  
Receptor Modulator ◽  
Igf I

OBJECTIVE: Some phytoestrogens are believed to have selective estrogen receptor modulator (SERM) activity with no action in the uterus but beneficial effects in the hypothalamo/pituitary unit and in the bone and are presently the focus of clinical interest. In the present experiments, the effects of the clinically used Cimicifuga racemosa (CR) extract BNO 1055 in the uterus, in the bone and on serum luteinizing hormone (LH) were compared with the effects of estradiol-17beta (E(2)) under acute and chronic conditions in ovariectomized rats. METHODS: Ovariectomized rats were treated either acutely (6 h) or chronically (3 Months) with E(2) or the CR extract. Gene expression of some estrogen-regulated genes in the metaphysis of the tibia and the uterus was determined. Furthermore, bone mineral density was measured by quantitative computer tomography. RESULTS: When given acutely, both E(2) and the CR extract inhibited LH secretion and slightly stimulated gene expression of IGF-I, collagen-1alpha1, osteoprotegerin and osteocalcin (all osteoblast products), and of tartrate-resistant acid phosphatase (TRAP, an osteoclast product) in the metaphysis of the femur. While E(2) stimulated uterine weight and expression of progesterone receptor (PR), the complement protein (C3) and IGF-I genes, and inhibited gene expression of the estrogen receptor beta (ERbeta) in the uterus, no such effect was observed under acute CR treatment. After chronic application with pelleted food over 3 Months E(2) had profound effects in the uterus on weight and gene expression (ERbeta, PR, C3 and IGF-I) which were not seen in the CR-treated animals. Within 3 Months after ovariectomy, control rats had lost more than 50% of the metaphyseal bone mass of the tibia, an effect prevented by E(2) and partially by CR supplementation. CONCLUSIONS: These data confirm the concept that the CR extract BNO 1055 contains as yet unidentified substances with SERM properties which act in the hypothalamo/pituitary unit and in the bone but not in the uterus.

scholarly journals Analysis of Gut Microbiota in Patients with Coronary Artery Disease and Hypertension

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Chuanqi Wan ◽  
Chen Zhu ◽  
Gulei Jin ◽  
Min Zhu ◽  
Junyi Hua ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Sensitivity ◽  
Cerebrovascular Diseases ◽  
Control Group ◽  
Clinical Indicator ◽  
Significant Difference ◽  
Healthy Control ◽  
Artery Disease ◽  
Dominant Bacteria ◽  
Microbiota Diversity

Cardiovascular and cerebrovascular diseases are characterized by high rates of morbidity and mortality. Microbiota is closely associated with cardiovascular disease. We aimed to comprehensively analyze the microbiotas of 300 healthy controls, 300 patients with high blood pressure (HBP), and 300 patients with coronary heart disease (CHD). The results indicated no significant difference in microbiota diversity among the three groups ( P > 0.05 ). However, differences in microbiota richness among the three groups were significant ( P < 0.05 ). Bacteroidetes and Bacteroidia were the dominant bacteria in the CHD group, Enterobacteriales and Escherichia-shigella in the HBP group, and Acidaminococcaceae and Phascolarctobacterium in the healthy control group. The prediction results of the random forest model indicated that the population with CHD displayed prominent features with high sensitivity, indicating that microbiota detection might become a novel clinical indicator to predict and monitor the risk of cardiovascular events. The prediction of microbiota function suggested differences in oxygen supply and chronic inflammation between populations with HBP/CHD and healthy populations. Although there is no difference in gut microbiota diversity among the three groups, each group has its dominant microbiota in terms of richness.

scholarly journals Association between Gut Microbiota and Infant’s Temperament in the First Year of Life in a Chinese Birth Cohort

2020 ◽  
Vol 8 (5) ◽  
pp. 753
Author(s):  
Ying Wang ◽  
Xiaoli Chen ◽  
Yun Yu ◽  
Yanqun Liu ◽  
Qing Zhang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Maternal Education ◽  
High Throughput Sequencing ◽  
Critical Role ◽  
First Year ◽  
Significant Difference ◽  
First Year Of Life ◽  
The Relationship ◽  
Microbiota Diversity ◽  
Gut Microbiota Composition

Infant temperament characteristics play a critical role in children’s developmental pathways and can predict adulthood psychopathology. The diversity and composition of the gut microbiota are associated with human temperament in both adults and young children. However, the relationship between the gut microbiota and temperament in 12-month-old infants is rarely studied; this developmental period is when temperament reaches a relatively stable stage. We used high-throughput sequencing methods to explore whether temperament characteristics were associated with gut microbiota diversity and composition. Infants’ fecal samples were collected at 12 months of age for the gut microbiota analysis. Based on the primary caregivers’ reports, infants’ temperaments were measured using the Infant Behavior Questionnaire-revised (IBQ-R). This study included 51 infants, including 20 boys and 31 girls, with a mean age of 12.25 months. Results showed that soothability was positively correlated with maternal education level (β = 0.29, p = 0.043, adjust p = 0.025) and the abundance of Bifidobacterium genera (β = 0.62, p = 0.004, adjust p = 0.002). Conversely, cuddliness was negatively correlated with the abundance of Hungatella genera. There was no significant difference in temperament based on gender. This study demonstrated that gut microbiota composition was associated with temperament in 12-month-old infants. These results point to the importance of gut microbiota balance. Future studies on the mechanisms behind the gut microbiota affecting temperament are warranted.

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