scholarly journals Studies on the Efficient Dual Performance of Lyngbya Majuscula Extract With Manganese Dioxide Nanoparticles in Photodegradation and Antimicrobial Activity

2021 ◽  
Author(s):  
RAMESH P ◽  
RAJENDRAN A ◽  
Manogar P
Keyword(s):  
Antimicrobial Activity ◽  
Manganese Dioxide ◽  
Micrococcus Luteus ◽  
Trichoderma Viride ◽  
Synthesis Method ◽  
Precipitation Method ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Lyngbya Majuscula ◽  
Manganese Dioxide Nanoparticles

Abstract Marine cyanobacteria Lyngbya Majuscula supported manganese dioxide-based novel green nanoparticle synthesised by simple precipitation method. The combination of microscopic and spectroscopic techniques we are using to characterise the synthesised Lyngbya Majuscula with manganese nanoparticles (LmMnO2NPs). The preparation of manganese dioxide nanoparticles is an entirely eco-friendly green synthesis method. The existence of biomolecule-based metal oxides was confirmed using Fourier-transform infrared (FTIR) spectroscopy. The XRD pattern confirms a crystalline nature and polydispersity. The optical transmission 269 nm using commonly used UV spectra and compute the optical band gap values of the material to be approximately 3.71 eV. The photodegradation study reveals manganese dioxide nanoparticles under LED light to 86% degradation within the 150 min of reaction. The standard volume of the synthesised manganese dioxide nanoparticles range was 115.8, and the DLS study confirms the 0.375 polydispersity index value. The green synthesised manganese dioxide nanoparticles obtained from the blue-green algae extract of Lyngbya Majuscula revealed potent antimicrobial activity Pseudomonas aeruginosa, Micrococcus luteus, Aspergillus niger, Staphylococcus aureus, Escherichia coli, and Trichoderma viride. In addition, the biosynthesised manganese dioxide nanoparticles may lead to better activity against the pathogenic microorganisms by the agar well diffusion method.

In vitro antimicrobial activity of Lemon grass oil by MIC and agar well diffusion method

2020 ◽  
Vol 1 (28) ◽  
pp. 80-86
Keyword(s):  
Antimicrobial Activity ◽  
Micrococcus Luteus ◽  
Diffusion Method ◽  
Food Spoilage ◽  
Zone Of Inhibition ◽  
Lemon Grass ◽  
Chemical Preservative ◽  
Cymbopogan Citratus ◽  
Agar Well Diffusion Method

Abstract-Foodpreservativesareusedtoincrease the shelf life of food and to maintain the quality for longer time. In the last scenario no herbal ayurvedic preservative had been considered with respect to the use of chemical preservative. Due to increasing demands for naturalandpreservativefreecompoundspromoted an idea of the replacement of synthetic preservatives with essential oils of antimicrobial properties.Essentialoilsfrommedicinalplantsarepotentialsourceofnovelantimicrobialcompounds especially against food spoilage pathogens. The aim of this project was to study antimicrobial activity of essential oil from Lemongrass (Cymbopogan citratus)oil against food spoilage organisms Escherichia coli, Micrococcus luteus, Staphylococcus aureus andBacillus cereus and fungus Aspergillus brasilliensis, Candida albicans, Chaetomium globosum and Penicillium funiculosumusing agar well diffusion method. The antimicrobial activity was evaluated by measuring the zone of inhibition. The oil at30% concentration completely/partially inhibited the growth of foodspoilagepathogens.Thesuccessfuleffectiveness of Lemon grass oil could also play amajorroleinreplacingthechemicalpreservative.

scholarly journals In-vitro Antimicrobial Activity of Roots of Rauwolfia serpentina L. Benth Kurz

2016 ◽  
Vol 8 (3) ◽  
pp. 312-316 ◽  
Author(s):  
Aniel K. OWK ◽  
Mutyala N. LAGUDU
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Micrococcus Luteus ◽  
Research Work ◽  
Microbial Pathogens ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Aqueous Extracts ◽  
Rauwolfia Serpentina

Microbial pathogens develop resistance to antibiotics after repeated administration during the treatment of infectious diseases. Therefore, it is necessary to find alternative antimicrobial drugs and the present trend is focused on medicinal plants. The hereby research work was carried out to investigate the antimicrobial activity of solvents as well as aqueous extracts of Rauwolfia serpentina roots. The extracts were tested against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Streptococcus pneumoniae, Aspergillus niger, Candida albicans and Saccharomyces cerevisiae by agar well diffusion method. It was observed that methanol extract showed the highest antimicrobial activity against multi drug resistance S. aureus at 100 mg/ml concentration, while S. aureus was the most susceptible bacterium to all extracts. However, E. faecalis, M. luteus and S. pneumoniae were also susceptible to the experimented solvents and extracts. On the other hand, K. pneumoniae was resistant against the solvent and aqueous extracts. The present study suggested that methanol extracts of R.  serpentina roots would be helpful in treating diseases caused by human pathogenic bacteria and fungi. In particular, based on the results obtained in the current experiment, it can be recommended for the control of infectious Gram-positive bacteria.

scholarly journals Antimicrobial Activity of Bacteriocin Produced By a New Lactobacillus Curvatus sp.LAB-3H Isolated From Traditional Yogurt

2021 ◽  
Author(s):  
Zahra Heidari ◽  
Mohammad Faezi Ghasemi ◽  
Leila Modiri
Keyword(s):  
Antimicrobial Activity ◽  
Micrococcus Luteus ◽  
Fold Increase ◽  
Exchange Chromatography ◽  
Food Preservation ◽  
Diffusion Method ◽  
Exponential Growth Phase ◽  
Potential Candidate ◽  
Lactobacillus Curvatus ◽  
16S Rrna Analysis

Abstract In recent years, the use of bacteriocin-producing Lactobacillus species has received much attention in different areas including using as probiotics, food preservation, and as broad antimicrobial spectrum activity. In this study, a bacteriocin-producing Lactobacillus strain was isolated from traditional yogurt. The isolate was identified by morphological, biochemical,16S rRNA analysis, and designated as Lactobacillus curvatus LAB-3H. The primary antimicrobial activity of the isolate was evaluated on Micrococcus luteus PTCC 1408 by the agar gel diffusion method. The growth of L. curvatus LAB-3H in MRS broth at 37°C and its antimicrobial activity against Lactobacillus casei ATCC 39392 was evaluated. In batch culture analysis, bacteriocin production starts at the early exponential growth phase and continues to the middle of the stationary phase about (660 AU/ml) at 28h and pH 3.8. Bacteriocin produced by L. curvatus LAB-3H showed antibacterial activity against some selected foodborne pathogens including; Listeria monocytogenes PTCC1294, Bacillus cereus PTCC1857, Staphylococcus aureus PTCC1917 and, Escherichia coli PTCC1276. For purification of bacteriocin, ammonium sulfate precipitation and cation exchange chromatography methods were used. The maximum antimicrobial activity observed was about 3985.15 AU/mg of protein, which was a 249.22-fold increase, and 5.21% yield compared with that in the cell-free supernatant. The molecular weight of bacteriocin was approximately 55 kDa by SDS-PAGE. The obtained results in this study demonstrate that bacteriocin from L. curvatus LAB-3H is a potential candidate for controlling microbial contaminations and can be used in different sectors such as food industries.

scholarly journals Biogenic Synthesis of MnO2 Nanoparticles With Leaf Extract of Viola betonicifolia for Enhanced Antioxidant, Antimicrobial, Cytotoxic, and Biocompatible Applications

2021 ◽  
Vol 12 ◽  
Author(s):  
Haibin Lu ◽  
Xueyang Zhang ◽  
Shakeel Ahmad Khan ◽  
Wenqiang Li ◽  
Lei Wan
Keyword(s):  
Manganese Dioxide ◽  
Biologically Active ◽  
Spectroscopic Techniques ◽  
Plant Leaves ◽  
Biofilm Inhibition ◽  
Mno2 Nanoparticles ◽  
External Standard ◽  
Manganese Dioxide Nanoparticles ◽  
Inhibition Performance ◽  
Mcf 7

In this study, we propose to synthesize NPs using plant extract containing active biomedical components, with the goal of obtaining NPs that inherit the biomedical activities of the plant. Herein, we report the synthesis of manganese dioxide nanoparticles (VBLE-MnO2 NPs) using the leaves extract of Viola betonicifolia, in which the biological active plant’s secondary metabolites function as both reducing and capping agents. The synthesized NPs were successfully characterized with different spectroscopic techniques. The antibacterial, antifungal, and biofilm inhibition properties of the synthesized VBLE-MnO2 NPs were further explored against a variety of bacteria (Gram-positive and Gram-negative) and mycological species. Additionally, their antioxidant ability against linoleic acid peroxidation inhibition, cytobiocompatibility with hMSC cells, and cytotoxicity against MCF-7 cells were investigated compared to leaves extract and chemically synthesized manganese dioxide NPs (CH-MnO2 NPs). The results were demonstrated that the synthesized VBLE-MnO2 NPs presented excellent antibacterial, antifungal, and biofilm inhibition performance against all the tested microbial species compared to plant leaves extract and CH-MnO2 NPs. Moreover, they also exhibited significant antioxidant potential, which was comparable to the external standard (ascorbic acid); however, it was higher than plant leaves extract and CH-MnO2 NPs. Furthermore, the synthesized CH-MnO2 NPs displayed good cytobiocompatibility with hMSC cells compared to CH-MnO2 NPs. The enhanced antioxidant, antibacterial, antifungal, and biofilm inhibition efficacy as compared to CH-MnO2 NPs might be attributed to the synergistic effect of the VBLE-MnO2 NPs’ physical properties and the adsorbed biologically active phytomolecules from the leaves extract of V. betonicifolia on their surface. Thus, our study establishes a novel ecologically acceptable route for nanomaterials’ fabrication with increased and/or extra medicinal functions derived from their herbal origins.

Synthesis, Characterization and Antimicrobial Activity of Piperidine Derivatives

2019 ◽  
Vol 41 (2) ◽  
pp. 363-363
Author(s):  
Shaista Zafar Shaista Zafar ◽  
Shamim Akhtar Shamim Akhtar ◽  
Syed Imran Ali Syed Imran Ali ◽  
Nausheen Mushtaq Nausheen Mushtaq ◽  
Sabahat Naeem and Mohsin Ali Sabahat Naeem and Mohsin Ali
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Disc Diffusion ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Disc Diffusion Method ◽  
Titration Method ◽  
The Past ◽  
Potentiometric Titration Method

Synthesis of various piperidine derivatives having important biological and pharmacological potentials has been discussed in the past. In present study we reported the synthesis of benzoyl and sulphonyl derivatives by taking Piperidine-4-carboxamide as principal molecule. These compounds were characterized by various spectroscopic techniques such as NMR, FTIR and Mass spectrometry. Elemental composition was explored using CHN analyzer. Antimicrobial activity study of the synthesized compounds was performed using disc diffusion method. Dissociation constant (pKa) of the synthesized compounds were determined by potentiometric titration method. In addition. The findings of the study predicted good absorption of these newly synthesized compounds. Besides, compound III showed good antifungal activity which can be helpful in pharmacokinetics and pharmacodynamics approaches of antibiotics.

scholarly journals Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia

2019 ◽  
Vol 23 (1) ◽  
pp. 1
Author(s):  
Agustina Eko Susanti ◽  
Shanti Ratnakomala ◽  
Wibowo Mangunwardoyo ◽  
Puspita Lisdiyanti
Keyword(s):  
Antimicrobial Activity ◽  
Micrococcus Luteus ◽  
Human Life ◽  
Botanical Garden ◽  
Diffusion Method ◽  
Rrna Gene ◽  
Screening Process ◽  
Science Centre ◽  
Agar Media ◽  
Biological Taxa

    Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.

scholarly journals Antimicrobial activity of leaf and flower extracts of Lippia Nodiflora L. (Verbenacea)

2012 ◽  
Vol 52 (4) ◽  
pp. 401-403 ◽  
Author(s):  
Zahra Zare ◽  
Ahmed Majd ◽  
Taher Nejad Sattari ◽  
Alireza Iranbakhsh ◽  
Sedigue Mehrabian
Keyword(s):  
Antimicrobial Activity ◽  
Micrococcus Luteus ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Esherichia Coli

Abstract Antimicrobial activities of the methanolic extracts from the leaves and flowers of Lippia nodiflora L. (Verbenaceae), were studied by the disk diffusion method. The extracts showed antimicrobial impact on bacteria such as Bacillus subtilis, B. cereus, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, K. oxytoca and Esherichia coli as well as fungi such Aspergillus niger and Candida albicanse. The results showed that increasing concentrations of extracts increased the antimicrobial activities in all of the microorganisms. Bacteria were more sensitive than fungi, and gram positive bacteria were more sensitive than gram negative ones.

scholarly journals New Insight into the Chemical Composition, Antimicrobial and Synergistic Effects of the Moroccan Endemic Thymus atlanticus (Ball) Roussine Essential Oil in Combination with Conventional Antibiotics

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5850
Author(s):  
Ahmed Nafis ◽  
Marcello Iriti ◽  
Lahcen Ouchari ◽  
Fatima El Otmani ◽  
Najat Marraiki ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Synergistic Effect ◽  
Synergistic Effects ◽  
Micrococcus Luteus ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Conventional Antibiotics ◽  
And Control

This study reported the volatile profile, the antimicrobial activity and the synergistic potential of essential oil (EO) from the Moroccan endemic Thymus atlanticus (Ball) Roussine, in combination with the antibiotics ciprofloxacin and fluconazole for the first time, to the best of our knowledge. The EO chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis and the antimicrobial activity assessed by the disc diffusion method against three Gram positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and one clinical isolate, Klebsiella pneumonia). The antifungal activity was evaluated in four pathogenic yeasts (Candida albicans, C. glabrata, C. krusei and C. parapsilosis). The minimum inhibition concentration (MIC) and the synergistic effect with ciprofloxacin and fluconazole were determined by the two-fold dilution technique and checkerboard test, respectively. Twenty-one constituents were identified by GC-MS in the EO, including carvacrol (21.62%) and borneol (21.13%) as the major components. The EO exhibited a significant antimicrobial activity with inhibition zones ranging from 0.7 mm to 22 mm for P. aeruginosa and B. subtilis, respectively, and MIC values varying from 0.56 mg/mL to 4.47 mg/mL. The fractional inhibitory concentration index (FICI) values ranged from 0.25 to 0.50 for bacteria and from 0.25 to 0.28 for yeasts. The maximum synergistic effect was observed for K. pneumonia with a 256-fold gain of antibiotic MIC. Our results have suggested that EO from T. atlanticus may be used alone or in association with antibiotics as a new potential alternative to prevent and control the emergence of resistant microbial strains both in the medical field and in the food industry.

scholarly journals Structural, Morphological and Antimicrobial Activity of pure and Aluminum doped Zinc Sulfide Nanoparticles

2019 ◽  
Vol 2 (1) ◽  
pp. 17-21 ◽  
Author(s):  
M Sathishkumar ◽  
M Saroja ◽  
V Venkatachalam
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Sulphide ◽  
Precipitation Method ◽  
Diffusion Method ◽  
Sem Images ◽  
Disk Diffusion Method ◽  
Fungus Culture ◽  
Aluminum Sulphate ◽  
Zinc Sulfide Nanoparticles ◽  
Co Precipitation

Undoped and Aluminum (Al) doped Zinc Sulphide (ZnS) Nanoparticles (NPs) has been prepared by chemical co- precipitation method using plant zinc sulphate, Aluminum sulphate and Thiourea . The X-Ray Powder Diffraction (XRD) studies reveals Cubic structure for undoped and Aluminum doped ZnS NPs. Scanning Electron Microscopy (SEM) images shows formation of smooth surfaced ZnS NPs  and average particles size was found in the range of 17 nm from the result the Aluminum doped ZnS were influenced to increase ZnS NPs particle size.  The disk diffusion method was used to screen the antimicrobial activity of Aluminum doped ZnS NPs against different gram positive, gram negative bacterial and fungus culture from this investigation Aluminum doped ZnS NPs  have potential antimicrobial agent which was show excellent zone of inhibition (ZoI)  at different concentration against all tested microorganisms.

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