Antimicrobial Activity of Bacteriocin Produced By a New Lactobacillus Curvatus sp.LAB-3H Isolated From Traditional Yogurt

Abstract In recent years, the use of bacteriocin-producing Lactobacillus species has received much attention in different areas including using as probiotics, food preservation, and as broad antimicrobial spectrum activity. In this study, a bacteriocin-producing Lactobacillus strain was isolated from traditional yogurt. The isolate was identified by morphological, biochemical,16S rRNA analysis, and designated as Lactobacillus curvatus LAB-3H. The primary antimicrobial activity of the isolate was evaluated on Micrococcus luteus PTCC 1408 by the agar gel diffusion method. The growth of L. curvatus LAB-3H in MRS broth at 37°C and its antimicrobial activity against Lactobacillus casei ATCC 39392 was evaluated. In batch culture analysis, bacteriocin production starts at the early exponential growth phase and continues to the middle of the stationary phase about (660 AU/ml) at 28h and pH 3.8. Bacteriocin produced by L. curvatus LAB-3H showed antibacterial activity against some selected foodborne pathogens including; Listeria monocytogenes PTCC1294, Bacillus cereus PTCC1857, Staphylococcus aureus PTCC1917 and, Escherichia coli PTCC1276. For purification of bacteriocin, ammonium sulfate precipitation and cation exchange chromatography methods were used. The maximum antimicrobial activity observed was about 3985.15 AU/mg of protein, which was a 249.22-fold increase, and 5.21% yield compared with that in the cell-free supernatant. The molecular weight of bacteriocin was approximately 55 kDa by SDS-PAGE. The obtained results in this study demonstrate that bacteriocin from L. curvatus LAB-3H is a potential candidate for controlling microbial contaminations and can be used in different sectors such as food industries.

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Studies on the Efficient Dual Performance of Lyngbya Majuscula Extract With Manganese Dioxide Nanoparticles in Photodegradation and Antimicrobial Activity

10.21203/rs.3.rs-1102629/v1 ◽

2021 ◽

Author(s):

RAMESH P ◽

RAJENDRAN A ◽

Manogar P

Keyword(s):

Antimicrobial Activity ◽

Manganese Dioxide ◽

Micrococcus Luteus ◽

Trichoderma Viride ◽

Synthesis Method ◽

Precipitation Method ◽

Diffusion Method ◽

Spectroscopic Techniques ◽

Lyngbya Majuscula ◽

Manganese Dioxide Nanoparticles

Abstract Marine cyanobacteria Lyngbya Majuscula supported manganese dioxide-based novel green nanoparticle synthesised by simple precipitation method. The combination of microscopic and spectroscopic techniques we are using to characterise the synthesised Lyngbya Majuscula with manganese nanoparticles (LmMnO2NPs). The preparation of manganese dioxide nanoparticles is an entirely eco-friendly green synthesis method. The existence of biomolecule-based metal oxides was confirmed using Fourier-transform infrared (FTIR) spectroscopy. The XRD pattern confirms a crystalline nature and polydispersity. The optical transmission 269 nm using commonly used UV spectra and compute the optical band gap values of the material to be approximately 3.71 eV. The photodegradation study reveals manganese dioxide nanoparticles under LED light to 86% degradation within the 150 min of reaction. The standard volume of the synthesised manganese dioxide nanoparticles range was 115.8, and the DLS study confirms the 0.375 polydispersity index value. The green synthesised manganese dioxide nanoparticles obtained from the blue-green algae extract of Lyngbya Majuscula revealed potent antimicrobial activity Pseudomonas aeruginosa, Micrococcus luteus, Aspergillus niger, Staphylococcus aureus, Escherichia coli, and Trichoderma viride. In addition, the biosynthesised manganese dioxide nanoparticles may lead to better activity against the pathogenic microorganisms by the agar well diffusion method.

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IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BACTERIA HAVING ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i10.12338 ◽

2016 ◽

Vol 8 (10) ◽

pp. 111

Author(s):

Garima Sharma ◽

Shweta Dang ◽

Sanjay Gupta ◽

Reema Gabrani

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Micrococcus Luteus ◽

Antibiofilm Activity ◽

Potential Candidate ◽

Indicator Organisms ◽

Sequencing Data ◽

Bacterial Strains ◽

Rdna Sequencing ◽

Animal Farm

Objective: The aim of the current study was to isolate and identify the bacteriocinogenic strain exhibiting broad range antimicrobial activity and antibiofilm activity from soil of animal farms.Methods: In the current study, bacterial strains were isolated from soil of twelve different regions of animal farm all over India and screened for antimicrobial activity against Staphylococcus epidermidis, Micrococcus luteus, Pseudomonas fluorescence and Escherichia coli. Antibiofilm ability of these selected strains was checked on preformed biofilm of S. epidermidis and in addition biofilm disruption potential was also determined. The potent bacterial strain was identified at molecular level by 16S ribosomal DNA (rDNA) sequencing.Results: 30 out of 231 strains isolated from soil were selected on the basis of antibacterial activity against S. epidermidis. One potential candidate (GAS 101) exhibited ≥99% inhibition against S. epidermidis, M. luteus, P. fluorescence and E. coli and also showed antibiofilm activity. GAS 101 16S rDNA sequencing data identified it as Bacillus subtilis. The sequence of B. subtilis was submitted to genbank under accession no. KJ564301.Conclusion: B. subtilis GAS 101 isolated from soil of animal farm showed the antibacterial activity against all indicator organisms and also displayed antibiofilm activity against preformed biofilm and inhibited biofilm formation of S. epidermidis.

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Antimicrobial activity of bacteriocin like inhibitory substance (BLIS) and lactic acid bacteria (LAB) isolated from traditional fermented buffalo milk from West Sumatra, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/924/1/012082 ◽

2021 ◽

Vol 924 (1) ◽

pp. 012082

Author(s):

F Hayati ◽

T Yuliana ◽

T Rialita

Keyword(s):

Antimicrobial Activity ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Buffalo Milk ◽

Food Preservation ◽

Diffusion Method ◽

Milk Product ◽

Safe Alternative ◽

Lactobacillus Pentosus ◽

West Sumatra

Abstract Food preservation by chemical preservatives addition is not recommended in large quantities. However, lactic acid bacteria (LAB) metabolites can be used as a safe alternative. LAB can produce a type of antimicrobial peptide known as bacteriocin. One source of LAB that can be used as a bacteriocin-producing candidate is dadih, a traditional fermented buffalo milk product from West Sumatra, Indonesia. In this study, eight bacterial isolates isolated from dadih were identified using the catalase test and Gram straining and obtained 6 isolates that were taken as LAB. An antimicrobial activity test was carried out using the diffusion method on LAB liquid isolates suspension and crude bacteriocins from the six isolates. The isolates with the highest antimicrobial activity were DK8, the diameter of inhibiting zone of liquid isolates was 15.75 mm (Salmonella sp.), 14.25 mm (Escherichia coli), and 12.25 mm (Staphylococcus aureus). Meanwhile, the diameter of crude bacteriocin inhibition of DK 8 isolates was 8 mm (Salmonella sp.). 8 mm (E. coli), and 8.5 mm (S. aureus). Furthermore, 16S rRNA sequencing confirmed DK8 as Lactobacillus pentosus.

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In vitro antimicrobial activity of Lemon grass oil by MIC and agar well diffusion method

Universities' Journal of Phytochemistry and Ayurvedic Heights ◽

10.51129/ujpah-2020-28-1-12 ◽

2020 ◽

Vol 1 (28) ◽

pp. 80-86

Keyword(s):

Antimicrobial Activity ◽

Micrococcus Luteus ◽

Diffusion Method ◽

Food Spoilage ◽

Zone Of Inhibition ◽

Lemon Grass ◽

Chemical Preservative ◽

Cymbopogan Citratus ◽

Agar Well Diffusion Method

Abstract-Foodpreservativesareusedtoincrease the shelf life of food and to maintain the quality for longer time. In the last scenario no herbal ayurvedic preservative had been considered with respect to the use of chemical preservative. Due to increasing demands for naturalandpreservativefreecompoundspromoted an idea of the replacement of synthetic preservatives with essential oils of antimicrobial properties.Essentialoilsfrommedicinalplantsarepotentialsourceofnovelantimicrobialcompounds especially against food spoilage pathogens. The aim of this project was to study antimicrobial activity of essential oil from Lemongrass (Cymbopogan citratus)oil against food spoilage organisms Escherichia coli, Micrococcus luteus, Staphylococcus aureus andBacillus cereus and fungus Aspergillus brasilliensis, Candida albicans, Chaetomium globosum and Penicillium funiculosumusing agar well diffusion method. The antimicrobial activity was evaluated by measuring the zone of inhibition. The oil at30% concentration completely/partially inhibited the growth of foodspoilagepathogens.Thesuccessfuleffectiveness of Lemon grass oil could also play amajorroleinreplacingthechemicalpreservative.

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In-vitro Antimicrobial Activity of Roots of Rauwolfia serpentina L. Benth Kurz

Notulae Scientia Biologicae ◽

10.15835/nsb839831 ◽

2016 ◽

Vol 8 (3) ◽

pp. 312-316 ◽

Cited By ~ 2

Author(s):

Aniel K. OWK ◽

Mutyala N. LAGUDU

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Micrococcus Luteus ◽

Research Work ◽

Microbial Pathogens ◽

Diffusion Method ◽

Multi Drug Resistance ◽

Aqueous Extracts ◽

Rauwolfia Serpentina

Microbial pathogens develop resistance to antibiotics after repeated administration during the treatment of infectious diseases. Therefore, it is necessary to find alternative antimicrobial drugs and the present trend is focused on medicinal plants. The hereby research work was carried out to investigate the antimicrobial activity of solvents as well as aqueous extracts of Rauwolfia serpentina roots. The extracts were tested against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Streptococcus pneumoniae, Aspergillus niger, Candida albicans and Saccharomyces cerevisiae by agar well diffusion method. It was observed that methanol extract showed the highest antimicrobial activity against multi drug resistance S. aureus at 100 mg/ml concentration, while S. aureus was the most susceptible bacterium to all extracts. However, E. faecalis, M. luteus and S. pneumoniae were also susceptible to the experimented solvents and extracts. On the other hand, K. pneumoniae was resistant against the solvent and aqueous extracts. The present study suggested that methanol extracts of R. serpentina roots would be helpful in treating diseases caused by human pathogenic bacteria and fungi. In particular, based on the results obtained in the current experiment, it can be recommended for the control of infectious Gram-positive bacteria.

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Antimicrobial Activity of Methanolic Root Extract of Decalepis hamiltonii Wight & Arn

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.56.52 ◽

2016 ◽

Vol 56 ◽

pp. 52-56

Author(s):

P. Prakash ◽

R. Manivasagaperumal

Keyword(s):

Antimicrobial Activity ◽

Fungal Species ◽

Food Preservation ◽

Diffusion Method ◽

Root Extract ◽

Flavonoid Content ◽

Zone Formation ◽

Root Extracts ◽

Decalepis Hamiltonii ◽

Phenolic And Flavonoid

The present study was quantitative analysis and antimicrobial activity of petroleum ether, chloroform, ethyl acetate, methanol root extracts. The gram positive and gram negative bacterial and fungal species by the disc diffusion method at various concentration (250μl-1000μl). The results was present in the phenolic and flavonoid content compared to extracts. All the root extract against to Bacillus subtilis and Streptococcus pyogenes and fungal species Candida albicans at highest inhibition at concentration of methanolic root extract of zone formation. The preliminary studies on Decalepis hamiltonii root extract exhibited their antimicrobial potential which could be exploited further and future pharmaceutical, therapeutic efficiency and food preservation and applications.

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Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia

ANNALES BOGORIENSES ◽

10.14203/ann.bogor.2019.v23.n1.1-12 ◽

2019 ◽

Vol 23 (1) ◽

pp. 1

Author(s):

Agustina Eko Susanti ◽

Shanti Ratnakomala ◽

Wibowo Mangunwardoyo ◽

Puspita Lisdiyanti

Keyword(s):

Antimicrobial Activity ◽

Micrococcus Luteus ◽

Human Life ◽

Botanical Garden ◽

Diffusion Method ◽

Rrna Gene ◽

Screening Process ◽

Science Centre ◽

Agar Media ◽

Biological Taxa

Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.

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Antimicrobial activity of leaf and flower extracts of Lippia Nodiflora L. (Verbenacea)

Journal of Plant Protection Research ◽

10.2478/v10045-012-0065-9 ◽

2012 ◽

Vol 52 (4) ◽

pp. 401-403 ◽

Cited By ~ 4

Author(s):

Zahra Zare ◽

Ahmed Majd ◽

Taher Nejad Sattari ◽

Alireza Iranbakhsh ◽

Sedigue Mehrabian

Keyword(s):

Antimicrobial Activity ◽

Micrococcus Luteus ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Methanolic Extracts ◽

Esherichia Coli

Abstract Antimicrobial activities of the methanolic extracts from the leaves and flowers of Lippia nodiflora L. (Verbenaceae), were studied by the disk diffusion method. The extracts showed antimicrobial impact on bacteria such as Bacillus subtilis, B. cereus, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, K. oxytoca and Esherichia coli as well as fungi such Aspergillus niger and Candida albicanse. The results showed that increasing concentrations of extracts increased the antimicrobial activities in all of the microorganisms. Bacteria were more sensitive than fungi, and gram positive bacteria were more sensitive than gram negative ones.

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New Insight into the Chemical Composition, Antimicrobial and Synergistic Effects of the Moroccan Endemic Thymus atlanticus (Ball) Roussine Essential Oil in Combination with Conventional Antibiotics

Molecules ◽

10.3390/molecules26195850 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5850

Author(s):

Ahmed Nafis ◽

Marcello Iriti ◽

Lahcen Ouchari ◽

Fatima El Otmani ◽

Najat Marraiki ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Synergistic Effect ◽

Synergistic Effects ◽

Micrococcus Luteus ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Conventional Antibiotics ◽

And Control

This study reported the volatile profile, the antimicrobial activity and the synergistic potential of essential oil (EO) from the Moroccan endemic Thymus atlanticus (Ball) Roussine, in combination with the antibiotics ciprofloxacin and fluconazole for the first time, to the best of our knowledge. The EO chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis and the antimicrobial activity assessed by the disc diffusion method against three Gram positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and one clinical isolate, Klebsiella pneumonia). The antifungal activity was evaluated in four pathogenic yeasts (Candida albicans, C. glabrata, C. krusei and C. parapsilosis). The minimum inhibition concentration (MIC) and the synergistic effect with ciprofloxacin and fluconazole were determined by the two-fold dilution technique and checkerboard test, respectively. Twenty-one constituents were identified by GC-MS in the EO, including carvacrol (21.62%) and borneol (21.13%) as the major components. The EO exhibited a significant antimicrobial activity with inhibition zones ranging from 0.7 mm to 22 mm for P. aeruginosa and B. subtilis, respectively, and MIC values varying from 0.56 mg/mL to 4.47 mg/mL. The fractional inhibitory concentration index (FICI) values ranged from 0.25 to 0.50 for bacteria and from 0.25 to 0.28 for yeasts. The maximum synergistic effect was observed for K. pneumonia with a 256-fold gain of antibiotic MIC. Our results have suggested that EO from T. atlanticus may be used alone or in association with antibiotics as a new potential alternative to prevent and control the emergence of resistant microbial strains both in the medical field and in the food industry.

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Characteristics of the etiological structure and susceptibility to antibiotics, antiseptics of infectious pathogens of respiratory organs in children in critical states

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2018-22(2)-16 ◽

2018 ◽

Vol 22 (2) ◽

pp. 311-317

Author(s):

O.A. Nazarchuk ◽

A.I. Starodub ◽

O.V. Rymsha ◽

V.A. Starodub ◽

S.A. Kolodii

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Agents ◽

Inflammatory Diseases ◽

Diffusion Method ◽

Chlorhexidine Digluconate ◽

Gram Positive ◽

Critical States ◽

Results Of Treatment ◽

Moderate Resistance ◽

Staphylococcus Spp

The study of the etiological structure, the properties of pathogens of the respiratory infectious diseases in children and their resistance to antibacterial agents is particularly relevant in modern conditions, expands the search for new approaches to combating pathogens, improves the results of treatment and reduces the mortality of this pathology. The aim — study of etiological structure, sensitivity to antibiotics and antiseptics of pathogens of infectious and inflammatory diseases of respiratory organs in children. In the study there were enrolled 247 patients who were treated in Vinnytsia Regional Children’s Clinical Hospital (VRCCH) in 2016. The sensitivity of microorganisms to 23 antibacterial agents was determined by the disc-diffusion method according to the generally accepted method. The analysis of the antimicrobial activity of antiseptic drugs (decamethoxine, miramistin, chlorhexidine digluconate) was performed by a double serial dilution technique with the determination of the minimum inhibitory bacteriostatic (MIC) and bactericidal (MBcC) concentrations, by the method of successive serial dilutions of the drug in a liquid nutrient medium. In patients who were in inpatient treatment at the VRCCH in 2016 because of pneumonia there were found opportunistic microorganisms which were of etiological significance in the development of the infection. Among them there were Streptococci (47,3 %), Staphylococci (15,3 %), Candida (13,3 %), Enterococci (10,9 %), including a high proportion of owned non-fermenting gram negative bacilli (9,8%) and species of Enterobacteria (2,0 %). Isolated strains of microorganisms had moderate resistance to most modern antibiotic drugs. The sensitivity of isolated strains of microorganisms to reserved antibiotics as carbapenems, often being used in the treatment of critical states of patients in the intensive care units, was found to above 18,2%. The sensitivity to this antibiotic in Enterococcus spp. (7,1 %), Staphylococcus spp. (5,9 %) was also low. Carbapenems, fluoroquinolones (the 1st and 2nd generations), antibiotics and aminoglycosides were found to be effective against gram positive microorganisms in more then 45% of cases. According to this they were considered to be as drugs of choice in the treatment of infectious and purulent-inflammatory pathology of respiratory organs, caused metitcilin- and vancomycin-resistant strains of microorganisms. Resistance to these drugs among investigated strains did not exceed 9,0 %. The high bactericidal properties of antiseptics as decamethoxine was determined against S.pyogenes, Staphylococcus spp. Its MBcC against these bacteria (1,65±0,20 mkg/ml and 4,32±0,50 mkg/ml, respectively) proved the advantage of decamethoxine’s effectiveness in comparison with chlorhexidine digluconate 3,14 times, 2,44 times miramistin. Clinical strains of C.albicans showed the highest susceptibility to decamethoxine, which fungicidal activity was determined in the presence (16,17±2,33 mkg/ml), in comparison with chlorxedine (MFtsK 27,59±3,59 mg/ml) and miramistin activity (27,59±3,595 mkg/ml). In children with inflammatory diseases of the respiratory organs gram-positive cocci are among the predominant pathogens (73,5 %) of cases, in the association allocated – 8,0 % of pathogens. Allocated strains of microorganisms were moderately resistant to all antibiotics studied. For antimicrobial activity antiseptic drugs, especially decamethoxine, have advantages over antibiotics confirming the possibility of their use in combination with systemic antibacterials.

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