BSA‑Seq and Genetic Mapping Reveals AhRt2 as a Candidate Gene Responsible for Red Testa of Peanut

2021 ◽  
Author(s):  
Kun Zhang ◽  
Mei Yuan ◽  
Han Xia ◽  
Liangqiong He ◽  
Jing Ma ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Linkage Mapping ◽  
Sequence Variation ◽  
Diagnostic Marker ◽  
Recessive Gene ◽  
Single Recessive Gene ◽  
The Third ◽  
Important Trait ◽  
Testa Color ◽  
Insight Into

Abstract Testa color is an important trait of peanut (Arachis hypogaea L.). Peanuts with red testa are rich in anthocyanin, are very popular with consumers. However, genes responsible for the red testa trait in peanut are rarely reported. In order to fine map red testa gene, two F4 populations were constructed through the cross of YZ9102 (pink testa) with ZH12 (red testa) and Zhanhong2 (red testa). Genetic analysis indicated that red testa was controlled by a single recessive gene, and named as AhRt2 (Red testa gene 2). Using BSA-seq approach, AhRt2 was preliminary identified in chromosome 12, and further mapped to a 530-kb interval using 220 recombinant lines through linkage mapping. Functional annotation, expression profiling, and sequence variation analyses confirmed that the anthocyanin reductase (ANR), Arahy.IK60LM, was the most likely candidate gene for AhRt2. A SNP in the third exon of AhRt2 changed the encoding amino acids, was associated with red testa of peanut. In addition, a closely linkaged molecular marker to red testa trait was developed. Our result provide insight into the molecular mechanism underlying peanut testa color and provide valuable diagnostic marker for marker-assisted selected (MAS) breeding in peanut.

No fear of George Kingsley Zipf

10.1558/37291 ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 242-263
Author(s):  
Stefano Rastelli ◽  
Kook-Hee Gil
Keyword(s):  
Minimalist Program ◽  
Classroom Research ◽  
Language Design ◽  
Efficient Computation ◽  
Generative Linguistics ◽  
The Third ◽  
Factor Effect ◽  
Language Classroom ◽  
Recent Developments ◽  
Insight Into

This paper offers a new insight into GenSLA classroom research in light of recent developments in the Minimalist Program (MP). Recent research in GenSLA has shown how generative linguistics and acquisition studies can inform the language classroom, mostly focusing on what linguistic aspects of target properties should be integrated as a part of the classroom input. Based on insights from Chomsky’s ‘three factors for language design’ – which bring together the Faculty of Language, input and general principles of economy and efficient computation (the third factor effect) for language development – we put forward a theoretical rationale for how classroom research can offer a unique environment to test the learnability in L2 through the statistical enhancement of the input to which learners are exposed.

Marketing Responsible Drinking Effectively to Young Adults

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Colby Doyle ◽  
Matthew Gaudet ◽  
Dominic Lay ◽  
Amber McLeod ◽  
Robert Schaeffer
Keyword(s):  
Quantitative Research ◽  
Drinking Patterns ◽  
Major Question ◽  
The Public ◽  
The Third ◽  
Current Consumption ◽  
Sample Data ◽  
Vague Quantifiers ◽  
Insight Into ◽  
Alberta Health

The primary goal of this research is to identify and examine the components of responsible drinking advertisements. We will examine industry and government related advertisements as we try to understand one of our major questions: does the source influence the validity of the message? The next group of major questions that we will be looking to answer is how are the vague quantifiers used in responsible drinking campaigns interpreted by the public?  How many drinks do people consider “too much?” What does “drink responsibly” really mean? The third major question is whether or not an individual’s current consumption patterns of alcohol have any effect on how individuals assess responsible drinking campaigns. Our qualitative research has indicated that social influences can be strongly related with drinking patterns; this will be further examined in our quantitative research. Also, we will be looking into some of the psychology behind industry and government sponsored advertisements as well as gathering and interpreting information from a sample of our target demographic. Our target demographic consists of both male and females between the ages 18-24. Our literature review and qualitative analysis gave us good insight into some of the potential answers to our questions. We will use these potential answers from our previous research to guide us as we attempt to conduct conclusive research based on a sample data of 169 individuals. Our findings will aid us in developing conclusions and recommendations for Alberta Health Services.

Attitudes of Elderly People in the South Moravian Region to Further Education

2014 ◽  
Vol 4 (2) ◽  
pp. 54-78
Author(s):  
Petr Adamec ◽  
Marián Svoboda
Keyword(s):  
Quality Of Life ◽  
Elderly People ◽  
Further Education ◽  
The South ◽  
The Third ◽  
Interesting Insight ◽  
Survey Results ◽  
Insight Into ◽  
Demographic Features

This paper deals with the results of sociological survey focused on identification of the attitudes of elderly people to further education. The research was carried out in September 2010. Experience of elderly people with further education, their readiness (determination) for further education as well as their motivation and barriers in further education were also subjects of this research. Detecting elderly population’s awareness of universities of the third age and finding out their further education preferences were an integral part of the research. Research sample consisted of citizens over 55 years living in the South Moravian region. The survey results are structured by socio-demographic features e.g.: age, sex, educational attainment etc. and provide an interesting insight into the attitudes of the target group to one of the activities that contributes to improvement of their quality of life.

scholarly journals Single Nucleotide Polymorphism in SMAD7 and CHI3L1 and Colorectal Cancer Risk

2018 ◽  
Vol 2018 ◽  
pp. 1-23 ◽  
Author(s):  
Amal Ahmed Abd El-Fattah ◽  
Nermin Abdel Hamid Sadik ◽  
Olfat Gamil Shaker ◽  
Amal Mohamed Kamal
Keyword(s):  
Colorectal Cancer ◽  
Genetic Variation ◽  
Sequence Variation ◽  
Regulatory Protein ◽  
Nucleotide Polymorphisms ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
The Third ◽  
Common Cancer ◽  
Cancer Initiation

Colorectal cancer (CRC) is one of the leading cancers throughout the world. It represents the third most common cancer and the fourth in mortality. Most of CRC are sporadic, arise with no known high-penetrant genetic variation and with no previous family history. The etiology of sporadic CRC is considered to be multifactorial and arises from the interaction of genetic variants of low-penetrant genes and environmental risk factors. The most common well-studied genetic variation is single nucleotide polymorphisms (SNPs). SNP arises as a point mutation. If the frequency of the sequence variation reaches 1% or more in the population, it is referred to as polymorphism, but if it is lower than 1%, the allele is typically considered as a mutation. Lots of SNPs have been associated with CRC development and progression, for example, genes of TGF-β1 and CHI3L1 pathways. TGF-β1 is a pleiotropic cytokine with a dual role in cancer development and progression. TGF-β1 mediates its actions through canonical and noncanonical pathways. The most important negative regulatory protein for TGF-β1 activity is termed SMAD7. The production of TGF-βcan be controlled by another protein called YKL-40. YKL-40 is a glycoprotein with an important role in cancer initiation and metastasis. YKL-40 is encoded by the CHI3L1 gene. The aim of the present review is to give a brief introduction of CRC, SNP, and examples of some SNPs that have been documented to be associated with CRC. We also discuss two important signaling pathways TGF-β1 and CHI3L1 that influence the incidence and progression of CRC.

scholarly journals Fine Mapping and Candidate Gene Identification of a White Flower Gene BrWF3 in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

2021 ◽  
Vol 12 ◽  
Author(s):  
Shuangjuan Yang ◽  
Xinxin Tian ◽  
Zhiyong Wang ◽  
Xiaochun Wei ◽  
Yanyan Zhao ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Chinese Cabbage ◽  
Fine Mapping ◽  
Protein Function ◽  
Recessive Gene ◽  
Flower Color ◽  
White Flower ◽  
Transmission Electron Microscopy Analysis ◽  
Electron Microscopy Analysis ◽  
Xanthophyll Esters

Flower color is an important trait in plants. However, genes responsible for the white flower trait in Chinese cabbage are rarely reported. In this study, we constructed an F2 population derived from the Y640-288 (white flower) and Y641-87 (yellow flower) lines for the fine mapping of the white flower gene BrWF3 in Chinese cabbage. Genetic analysis indicated that BrWF3 was controlled by a single recessive gene. Using BSA-seq and KASP assays, BrWF3 was fine-mapped to an interval of 105.6 kb. Functional annotation, expression profiling, and sequence variation analyses confirmed that the AtPES2 homolog, Bra032957, was the most likely candidate gene for BrWF3. Carotenoid profiles and transmission electron microscopy analysis suggested that BrWF3 might participate in the production of xanthophyll esters (particularly violaxanthin esters), which in turn disrupt chromoplast development and the formation of plastoglobules (PGs). A SNP deletion in the third exon of BrWF3 caused the loss of protein function, and interfered with the normal assembly of PGs, which was associated with reduced expression levels of genes involved in carotenoid metabolism. Furthermore, we developed and validated the functional marker TXBH83 for BrWF3. Our results provide insight into the molecular mechanism underlying flower color pigmentation and reveal valuable information for marker-assisted selection (MAS) breeding in Chinese cabbage.

scholarly journals Impact of COVID-19 Variants on the Second Wave in India and Subsequent Discretion of Possibilities of the Third Wave

2021 ◽  
pp. 115-126
Author(s):  
Sudarshan Ramaswamy ◽  
Meera Dhuria ◽  
Sumedha M. Joshi ◽  
Deepa H Velankar
Keyword(s):  
Herd Immunity ◽  
Sir Model ◽  
The Other ◽  
Third Wave ◽  
Focal Points ◽  
Current Analysis ◽  
The Third ◽  
The Impact ◽  
Second Wave ◽  
Insight Into

Introduction: Epidemiological comprehension of the COVID-19 situation in India can be of great help in early prediction of any such indications in other countries and possibilities of the third wave in India as well. It is essential to understand the impact of variant strains in the perspective of the rise in daily cases during the second wave – Whether the rise in cases witnessed is due to the reinfections or the surge is dominated by emergence of mutants/variants and reasons for the same. Overall objective of this study is to predict early epidemiological indicators which can potentially lead to COVID-19 third wave in India. Methodology: We analyzed both the first and second waves of COVID-19 in India and using the data of India’s SARS-CoV-2 genomic sequencing, we segregated the impact of the Older Variant (OV) and the other major variants (VOI / VOC).  Applying Kermack–McKendrick SIR model to the segregated data progression of the epidemic in India was plotted in the form of proportion of people infected. An equation to explain herd immunity thresholds was generated and further analyzed to predict the possibilities of the third wave. Results: Considerable difference in ate of progression of the first and second wave was seen. The study also ascertains that the rate of infection spread is higher in Delta variant and is expected to have a higher threshold (>2 times) for herd immunity as compared to the OV. Conclusion: Likelihood of the occurrence of the third wave seems unlikely based on the current analysis of the situation, however the possibilities cannot be ruled out. Understanding the epidemiological details of the first and second wave helped in understanding the focal points responsible for the surge in cases during the second wave and has given further insight into the future.

scholarly journals An Analytical study of Bediuzzaman Said Nursi’s Intellectual Development and Composition of Risale-i Nur (a Thematic Qur’ānic Exegesis)

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Dr. Shumaila Majeed
Keyword(s):  
Intellectual Development ◽  
Analytical Study ◽  
Muslim Community ◽  
The Third ◽  
Life Education ◽  
Representative Work ◽  
Qur'anic Exegesis ◽  
Islamic Belief ◽  
Distinguishing Features ◽  
Insight Into

The current study analyses Bediuzzaman Said Nursi’s life and contributions. It presents a short description of his early life, education and dissatisfaction with the then prevailing education systems. This is followed by an analysis of his intellectual development due to rapidly changing circumstances. The paper also presents an insight into Nursi’s representative work ‘Risale-i Nur’ through an analysis of its structure and methodology. This is significant because his intellectual development, due to changes in his circumstances, played an instrumental role in determining the style, method and objectives of Risale. Hence, the study of his intellectual development is imperative to understand the objectives of Risale. The findings of the paper revealed that Nursi’s approach to reform the Muslim society was not stagnant. In the ‘Old Said’ phase, he attempted to neutralize the decline in Muslim community through sociopolitical reforms. The ‘New Said’ phase was marked by his efforts to strengthen belief, encounter anti-religious ideologies and detachment from politics. In the ‘Third Said’ phase, he again took interest in sociopolitical matters but it was limited to the theoretical realm with no practical involvement. The study also found that the structure of Risale, its treatment of subjects and its methodology are its distinguishing features. It consists of the elements well-suited to the needs of the time to save Islamic belief against the onslaught of atheistic ideologies. The current research was conducted using the qualitative approach. It is beneficial for academia in understanding the intellectual development of this great 20th century Turkish Muslim scholar.

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