Preliminary Phytochemical Screening, Isolation, Characterization, Structural Elucidation and Antibacterial Activities of Leaves Extracts Rhus Vulgaris (Kimmo)

2021 ◽  
Author(s):  
Aderaw Anteneh ◽  
Getachew G/Mariam W/Hana ◽  
Desta Shumuye Meshesha
Keyword(s):  
Antibacterial Activity ◽  
1H Nmr ◽  
13C Nmr ◽  
Antibacterial Activities ◽  
Solvent Mixture ◽  
Solvent Ratio ◽  
Herbal Plants ◽  
Rf Values ◽  
Phytochemical Constituents ◽  
Isolated Compound

Abstract Background: Rhus vulgaris commonly known as sumac, a plant that is known to possess different therapeutic values including antioxidant and antibacterial activities. Medicines from plants contributed largely to human health. The aim of this study was to screen the phytochemical constituents, isolate, elucidate the structure and antibacterial activity of methanol extract from the leaves of Rhus vulgaris.Methods: The methanolic extract of Rhus vulgaris was subjected to column chromatography and eluted with solvent mixture of methanol: chloroform (1:8) ratio. The eluted fractions were run in the TLC mobile phase with the different solvent ratio. Based on the TLC profile the fractions with similar Rf values were pooled together. The structure of the isolated compound was characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Rhus vulgaris has been shown to have antibacterial activity were tested against four strains bacteria Streptococcus aureus(gram-positive) and Escherichia coli, Salmonella typhimurium, and K. pneumoniae (gram-negative) using Agar well diffusion method.Result: The results showed that the methanol extracts were active against all the tested bacteria. The structure of this compound 1-p-tolyl pentadeca-7,9-dien-1-ol was characterized by means of 1H NMR, 13C NMR, and IR spectral data.Conclusion: Therefore, it is concluded that the use of herbal plants and their recipes are the major source of drugs in a traditional medicinal system to cure different diseases.

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

scholarly journals Phytochemical constituents of the flowers of Sarcococca coriacea of Nepalese origin

1970 ◽  
Vol 21 ◽  
pp. 1-7 ◽  
Author(s):  
N. P. Rai ◽  
B. B. Adhikari ◽  
Arjun Paudel ◽  
K. Masuda ◽  
R. D. Mckelvey ◽  
...  
Keyword(s):  
Melting Point ◽  
Spectral Data ◽  
Oleanolic Acid ◽  
1H Nmr ◽  
13C Nmr ◽  
Chemical Society ◽  
Chromatographic Technique ◽  
Sugar Alcohol ◽  
Steroidal Glycosides ◽  
Phytochemical Constituents

From the flowers of Sarcococca coriacea, a triterpenoic acid, oleanolic acid, a pentahydric sugar alcohol, xylitol along with the mixture of steroidal glycosides, stigmasterol-3-O-β-D-glucopyranoside and β-sitosterol-3-O-β-D-glucopyranoside have been isolated by chromatographic technique. Their structures were established on the basis of IR, 1H-NMR, 13C-NMR, spectral data as well as melting point and Co-TLC comparison with the authentic samples. DOI: 10.3126/jncs.v21i0.214Journal of Nepal Chemical Society Vol.21 2006 p.1-7

scholarly journals Antibacterial Activity of Germacrane Type Sesquiterpenes from Curcuma heyneana Rhizomes

2014 ◽  
Vol 14 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Hartiwi Diastuti ◽  
Yana Maolana Syah ◽  
Lia Dewi Juliawaty ◽  
Marlia Singgih
Keyword(s):  
Antibacterial Activity ◽  
Liquid Chromatography ◽  
1H Nmr ◽  
13C Nmr ◽  
Spectroscopic Data ◽  
Microdilution Method ◽  
Vacuum Liquid Chromatography ◽  
Weak Antibacterial Activity

The isolation of terpenoids from C. heyneana rhizomes and their antibacterial activity have been conducted. The terpenoids were isolated by using vacuum liquid chromatography and radial chromatography. The structures of the compounds were determined based on spectroscopic data (1H-NMR, 13C-NMR (1D and 2D)). The antibacterial activity was carried out by using microdilution method and evaluated against eight bacteria. Three germacrane type sesquiterpenes have been isolated from C. heyneana rhizhomes and were identified as germacrone, dehydrocurdione, and 1(10),4(5)-diepoxygermacrone. Germacrone showed highest antibacterial activity against P. aeruginosa with MIC values of 15.6 µg/mL and MBC values 31.2 µg/mL. Dehydrocurdione showed highest antibacterial activity against B. subtilis with MIC values of 31.2 µg/mL and MBC values of 31.2 µg/mL. However, 1(10),4(5)-diepoxygermacrone showed a weak antibacterial activity.

scholarly journals New S-alkylated 1,2,4-triazoles incorporating diphenyl sulfone moieties with potential antibacterial activity

2009 ◽  
Vol 74 (10) ◽  
pp. 1041-1049 ◽  
Author(s):  
Stefania Barbuceanu ◽  
Gabriela Almajan ◽  
Ioana Saramet ◽  
Constantin Draghici ◽  
Radu Socoteanu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
1H Nmr ◽  
Elemental Analysis ◽  
13C Nmr ◽  
Ethyl Bromide ◽  
Phenacyl Bromide ◽  
Antibacterial Effects ◽  
Ethyl Chloroacetate ◽  
Diphenyl Sulfone ◽  
New Compounds

Alkylation of the 5-{4-[(4-bromophenyl)sulfonyl]phenyl}-4-(3/4-methylphenyl)- 2,4-dihydro-3H-1,2,4-triazole-3-thiones 3a,b with various alkylation agents, i.e., ethyl bromide, phenacyl bromide and ethyl chloroacetate, afforded the S-substituted 1,2,4-triazoles 4-6a,b. The structures of these new compounds were elucidated by elemental analysis and IR, UV, 1H-NMR, 13C-NMR and MS spectroscopy. The newly synthesized products were tested for their antibacterial effects.

scholarly journals ISOLASI DAN ELUSIDASI STRUKTUR SENYAWA TRITERPENOID DARI KULIT BATANG AMBACANG (Mangifera foetida L.) SERTA UJI BRINE SHRIMP LETALITY TEST (BSLT)

2015 ◽  
Vol 9 (1) ◽  
pp. 1 ◽  
Author(s):  
Adlis Santoni ◽  
Sabariah ◽  
Mai Efdi
Keyword(s):  
Ethyl Acetate ◽  
1H Nmr ◽  
13C Nmr ◽  
Brine Shrimp ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Toxic Effects ◽  
Toxic Compound ◽  
Isolated Compound

A brine shrimp toxic compound has been isolated from Mangifera foetida L. steam bark. Based on spectroscopic data (IR,1H- NMR, 13C-NMR 1D and 2D), the structure of this compound was identified as mangiferenes B, C30H47O2. In this study, the n-hexane, ethyl acetate, methanol extracts and isolated compound showed toxic effects (LC50 471.9543; 534.5209, 678.4421, and 22.1615 μg/mL, respectively). The toxicity degree among the three extracts and isolated compound is isolated compound > n-hexane extract > ethyl acetate extract > methanol extract.

scholarly journals STRUCTURE ELUCIDATION OF ANTIBACTERIAL COMPOUND FROM Ficus deltoidea Jack LEAVES

2011 ◽  
Vol 11 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Suryati Suryati ◽  
Hazli Nurdin ◽  
Dachriyanus Dachriyanus ◽  
Md Nordin Hj Lajis
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Spectroscopic Data ◽  
Structure Elucidation ◽  
Antibacterial Activities ◽  
Ficus Deltoidea ◽  
E Coli ◽  
Minimum Inhibition Concentration ◽  
Antibacterial Compound ◽  
Inhibition Concentration

An antibacterial compound has been isolated from Ficus deltoidea Jack leaves. Based on spectroscopic data (IR, 1H-NMR, 13C NMR 1D and 2D and MS), the structure of this compound was identified as 3β-hydroksilup-20(29)-en, (lupeol), C30H50O. This compound showed antibacterial activities against E. coli, B. subtilis and S. aureus. The minimum inhibition concentration (MIC) against E. coli, B. subtilis and S. aureus are 150, 220 and 130 μg/mL respectively.

scholarly journals Sulfonyl thiosemicarbazones: synthesis, characterization and antibacterial activity

2021 ◽  
pp. 22-26
Author(s):  
Priyanka Yadav ◽  
Praveen Kumar ◽  
Ayaz Mahmood Dar
Keyword(s):  
Antibacterial Activity ◽  
1H Nmr ◽  
13C Nmr ◽  
Analytical Techniques ◽  
E Coli ◽  
Antibacterial Screening ◽  
Zones Of Inhibition ◽  
New Compounds

The reaction of sulfonyl ketones (1-3) with thiosemicarbazide under reflux conditions yielded the sulfonyl thiosemicarbazones (4-6) in good to potential amounts (72-80%). The structure of new compounds (4-6) was confirmed by different spectral (IR, 1H NMR, 13C NMR, MS) and analytical techniques. The investigation of antibacterial screening data revealed that all the tested compounds (4-6) exhibited moderate to decent antibacterial activity against S. pyogenes, S. aureus and E. coli strains. The compounds 4-6 depicted zones of inhibition, i.e., 20.3 mm, 21.2 mm and 19.6 mm against S. pyogenes strain, respectively.

scholarly journals Secondary Metabolite from Endophytic Fungi Aspergillus Sp. The Leave Of Kunyit Putih (Curcuma zedoaria (Berg) Roscoe)

Molekul ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 125 ◽  
Author(s):  
Muharni Muharni ◽  
Fitrya Fitrya ◽  
Widia Purwaningrum ◽  
Ahmad Yogi Nugraha
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
13C Nmr ◽  
Spectroscopic Method ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Curcuma Zedoaria ◽  
Potato Dextrose Broth ◽  
Aspergillus Sp ◽  
Isolated Compound

The compound from endophytic fungi of Aspergillus sp. from leaves of kunyit putih (Curcuma zedoaria (Berg.) Roscoe) has been isolated. Isolation begins with cultivation ofAspergillus sp. in 18 L PDB’s media (Potato Dextrose Broth) for 28 days. The liquid cultivation medium was extracted by partitioning method using ethylacetate and then evaporated. The extract was separated and purified by chromatography techniques. Elucidation stucture of the isolated compound was analysis by spectroscopic method NMR 1D and 2D. Antibacterial activity of isolated compound was tested using the disc diffusion method at concentrations 2500, 1000, 500, and 125 ppm. The isolated compounds obtained in the form of a yellow oil (24.30 mg). The 13C NMR spectrum indicated 24 signals of carbon and base on analysis spectrum DEPT 135 showed 5 signal methynes carbon, 1 signals methylene, 9 signals of methyl and 9 signals quarternary carbon. These signals from 1H and 13C-NMR suggested that this compound contained aromatic group and four carbonyl. The isolated compound show antibacterial activity at concentration 2500 ppm which inhibition zone for E. coli, S. dysenteriae, S. aureus, B. subtilis were 10.3 ; 8.3; 8.4; and 7.8 mm, respectively. Based on the analysis result of NMR 1D and 2D, the compound was methyl 6-(5'-(2"-acetoxy-2”-methylpropanoyl)-3"- methyl-2'-(3"'-methylbutanoyl)phenyl)-3-methylbutanoate and has weak antibacterial activity.

scholarly journals Isolation, Characterization and Antibacterial Activity of ECDY Steroid from Roots of Polycarpaea corymbosa LAM

2021 ◽  
Vol 68 (2) ◽  
Author(s):  
I. Carolin Nimila ◽  
K.G. Lalitha ◽  
R.Sambath kumar
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
13C Nmr ◽  
Bacterial Activity ◽  
Pure Compound ◽  
Simultaneous Extraction ◽  
Soxhlet Apparatus ◽  
Isolated Compound ◽  
Benzene Extract

A new phytoecdysteroid (2- deoxy 20 hydroxy 22 cinnamoyl ecdysone 3, 25 diacetate) was isolated from the roots of Polycarpaea corymbosa Lam (family: caryophyllaceae).The powdered roots were extracted with petroleum ether ( 60-80? C), benzene, ethyl acetate and ethyl alcohol in soxhlet apparatus by simultaneous extraction. The highest yield of benzene extract was chosen for the isolation of ecdysteroid. The white solid pure compound was isolated and tested by TLC and respond positively for Liebermann - burchard reaction. Their structure has been established by UV, IR, 1H, 13C-NMR DEPT -90, DEPT -135 and EI- MS experiments. All the extracts and isolated compound were screened for anti-bacterial activity against B.subtilis, P.vulgaris, E.coli, P.aeruginosa by Kirbybauer method.

scholarly journals New isoquinoline alkaloid from Carduus crispus L.

2014 ◽  
Vol 12 ◽  
pp. 85-87
Author(s):  
J Tunsag ◽  
G Davaakhuu ◽  
D Batsuren
Keyword(s):  
1H Nmr ◽  
Bioactive Compounds ◽  
13C Nmr ◽  
Ethanol Extract ◽  
Isoquinoline Alkaloid ◽  
Spectroscopic Methods ◽  
Aerial Parts ◽  
Isolated Compound ◽  
First Time

A new isoquinoline alkaloid, along with other known 4 bioactive compounds (1 isoquinoline alkaloid, 2 flavonoids, 1 coumarin), were isolated from the ethanol extract of aerial parts of Carduus crispus L. Crispine А N-oxide was newly isolated compound, two of them (quercetin and rutin) were compounds isolated for the first time from indicated plant. The structures of these compounds were elucidated and confirmed with spectroscopic methods e.g. 1H-NMR, 13C-NMR, EI-MS, COSY, HSQC, HMBC, and by comparison with literature data.DOI: http://dx.doi.org/10.5564/mjc.v12i0.178 Mongolian Journal of Chemistry Vol.12 2011: 85-87

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