scholarly journals Secondary Metabolite from Endophytic Fungi Aspergillus Sp. The Leave Of Kunyit Putih (Curcuma zedoaria (Berg) Roscoe)

Molekul ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 125 ◽  
Author(s):  
Muharni Muharni ◽  
Fitrya Fitrya ◽  
Widia Purwaningrum ◽  
Ahmad Yogi Nugraha
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
13C Nmr ◽  
Spectroscopic Method ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Curcuma Zedoaria ◽  
Potato Dextrose Broth ◽  
Aspergillus Sp ◽  
Isolated Compound

The compound from endophytic fungi of Aspergillus sp. from leaves of kunyit putih (Curcuma zedoaria (Berg.) Roscoe) has been isolated. Isolation begins with cultivation ofAspergillus sp. in 18 L PDB’s media (Potato Dextrose Broth) for 28 days. The liquid cultivation medium was extracted by partitioning method using ethylacetate and then evaporated. The extract was separated and purified by chromatography techniques. Elucidation stucture of the isolated compound was analysis by spectroscopic method NMR 1D and 2D. Antibacterial activity of isolated compound was tested using the disc diffusion method at concentrations 2500, 1000, 500, and 125 ppm. The isolated compounds obtained in the form of a yellow oil (24.30 mg). The 13C NMR spectrum indicated 24 signals of carbon and base on analysis spectrum DEPT 135 showed 5 signal methynes carbon, 1 signals methylene, 9 signals of methyl and 9 signals quarternary carbon. These signals from 1H and 13C-NMR suggested that this compound contained aromatic group and four carbonyl. The isolated compound show antibacterial activity at concentration 2500 ppm which inhibition zone for E. coli, S. dysenteriae, S. aureus, B. subtilis were 10.3 ; 8.3; 8.4; and 7.8 mm, respectively. Based on the analysis result of NMR 1D and 2D, the compound was methyl 6-(5'-(2"-acetoxy-2”-methylpropanoyl)-3"- methyl-2'-(3"'-methylbutanoyl)phenyl)-3-methylbutanoate and has weak antibacterial activity.

scholarly journals Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

2021 ◽  
Vol 8 (3) ◽  
pp. 24-28
Author(s):  
Puteri Amelia ◽  
Rachma Ayunda ◽  
Saiful Bahri
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacillus Subtilis Atcc

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF Curcuma longa AND Curcuma zedoaria RHIZOMES EXTRACTS AT A CONCENTRATION OF 12.5% AGAINST Streptococcus mutans

2021 ◽  
Vol 1 (Volume 1 No 2) ◽  
pp. 175-187
Author(s):  
Martha Mozartha ◽  
Muhammad Yusuf Diansyah ◽  
LIstia Eka Merdekawati
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Curcuma Longa ◽  
Inhibition Zone ◽  
Oral Bacteria ◽  
Diffusion Method ◽  
Control Group ◽  
Distilled Water ◽  
Curcuma Zedoaria ◽  
Mean Diameter

Streptococcus mutans is a microorganism that important in dental caries. Herbs are known to have antibacterial activity against oral bacteria. The rhizomes of Curcuma longa and Curcuma zedoaria are two of the eight species of genus Curcuma that are most widely used as traditional herbal medicine in Indonesia. Both rhizomes have antibacterial activity against oral bacteria at a concentration of 12.5%. This study aimed to examine the inhibitory of Curcuma longa and Curcuma zedoaria rhizomes extracts against the growth of Streptococcus mutants. The study was in vitro study. Both rhizomes got from the Experimental Garden of Balittro Manoko Lembang, West Java, Indonesia. The maceration was employed to obtain the extracts with the final extracts of both rhizomes at a concentration of 12.5%. Antibacterial activity test was carried out by using the agar well diffusion method. The positive control group was 0.2% Chlorhexidine and distilled water as a negative control group. The data obtained were then analyzed using the One Way ANOVA Test and Post Hoc Test. Results showed that chlorhexidine 0.2% had the largest mean diameter of the inhibition zone. Curcuma longa rhizome extract had a larger mean diameter of inhibition zone compared to Curcuma zedoaria rhizome extract. However, the difference in the mean values of the two rhizomes extracts was not statistically significant. Distilled water didn't have antibacterial activity. It can be concluded that both Curcuma longa and Curcuma zedoaria rhizomes extracts at a concentration of 12.5% have antibacterial activity against Streptococcus mutans.

scholarly journals Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root.

2017 ◽  
Vol 10 (4) ◽  
pp. 225 ◽  
Author(s):  
Shiv Kumar Verma ◽  
Moti Lal ◽  
Mira Debnath
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Sclerotium Rolfsii ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Calotropis Procera ◽  
Aspergillus Sp ◽  
Antimicrobial Metabolites

Objective:To study the antimicrobial activity of crud ethylacetate  extract from endophytic fungus in Calotropis procera root.Methods:Endophytic fungus was screened for production of antimicrobial metablites.fermentation was carried out in 500 ml Erlenmeyer flask. Disc diffusion method was used to test antimicrobial activity of crude extract using chloramphenicol sulphate and Flucnazole as positive controleRessults: A total of fourteen Endophytic fungi were isolated (CPR1- CPR14). Among these fourteen isolates, CPR5 was found to show maximum antimicrobial activity, in compare to other isolates, against gram positive, gram negative bacteria and fungi. The antimicrobial activity was tested against Escherichia coli. Pseudomonas aeroginosa, Bacillus subtilis, Staphylococcus aureus, Ralstonia solanacearum, Xanthomonas oryzae, Penicillium chrysogenum, Candida albicans, Phoma exigua, Sclerotium rolfsii and Sclerotinia scleratiourum. Minimum inhibitory concentration (MIC) of crude extract against test microorganisms was determined. Fungus was identified as aspergillus sp. Prduction parameters (Temprature, pH, Carbon source, Nitrgen surce, Sdium Chlride concentration) were optimized.Conclusion:  Crud  extract produced by the isolated endophytic fungus could be an important source of broad spectrum antimicrobial metabolites. Keywords: Endophytic fungi, Antimicrobial metabolites, Process optimization, Calotropis procera, Inhibition zone.

scholarly journals Antibacterial activity of endophytic fungi from some medicinal plants of Biligirirangana hill, India

2018 ◽  
Vol 10 (4) ◽  
pp. 1286-1290
Author(s):  
Sushma K.S ◽  
Jayashankar M. ◽  
Mohammed Ali Saeed ◽  
Vinu A.K.
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Inhibition Zone ◽  
Phytochemical Analysis ◽  
Crude Extracts ◽  
Aspergillus Sp ◽  
And Staphylococcus Aureus ◽  
Positive Results

Twenty fungal endophytes were isolated from different medicinal plants of Biligirirangana Hill, Chamarajanagar dist. Phytochemical analysis of ethyl acetate extract showed positive results for tanins, flavonoids, steroids, terpenoids, glycosides, saponins and alkaloids except saponin and flavonoid tests. The crude extracts of the fungal endophytes were tested against two gram positive and two gramnegetive bacteria for its antibacterial activity. The highest zone of inhibition was produced by Fusarium sps (AB9)35mm. All the crude extracts were found to be effective against Escherichia coli, Bacillus subtilis and Staphylococcus aureus rather in Pseudomonas aerogenosa. Among them the highest inhibition zone was produced in Penicillium sp (AB11) 24mm, Cladosporium sp(AB3) 21mm, and Aspergillus sp(AB12) 30mm. Therefore, endophytic fungi can be a good source to inhibit the growth of harmful pathogens.  

scholarly journals Isolasi dan Uji Aktivitas Antimikroba Kapang Endofit dari Kayu Ulin (Eusideroxylon zwageri Teijsm & Binn.) (Isolation and Antimicrobial Activity of Endophytic Fungi from Ulin (Eusideroxylon zwageri Teijsm & Binn.))

2017 ◽  
Vol 9 (2) ◽  
pp. 65-74
Author(s):  
Nadra Khairiah ◽  
Rinne Nintasari
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Antimicrobial Compound ◽  
Potato Dextrose Broth ◽  
E Coli ◽  
Antimicrobial And Antioxidant Activity ◽  
Eusideroxylon Zwageri

Endophytic fungi are reported to be potential as antibacterial, antifungal, antioxidant, anticancer, and antivirus. One of the source of endophytic fungus is ulin (Eusideroxylon zwageri Teijsm & Binn.) which produced the same antimicrobial compound with ulin wood itself. The purpose of this research was to isolate endophytic caps from ulin wood branch, and tested the antimicrobial and antioxidant activity. The isolates were fermented on PDB media (Potato Dextrose Broth), and then tested the antimicrobial activity on Eschericiacoli, Pseudomonas aeruginosa, and Enterococcus aerogenes). In this research, there were two endofit isolates (white/PT and greenish black/HT). HT endophytic isolate exhibited strong antibacterial activity against the two pathogens (P. aeruginosa and E. coli) shown in 12 mm inhibition zone, while PT endophytic isolate exhibited the E. coli in 11.5 mm inhibition zone. The highest antioxidant activity found in HT endophytic shell was 47.47%. Keywords : antimicrobial, endophytic, Eusideroxylon zwageri, fungi, ulin

Antibacterial Activity of Selenium Nanoparticles extracted from Capparis decidua against Escherichia coli and Lactobacillus Species

2021 ◽  
pp. 4452-4454
Author(s):  
Sneka S ◽  
Preetha Santhakumar
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Selenium Nanoparticles ◽  
Nano Particles ◽  
Diffusion Method ◽  
Lactobacillus Species ◽  
Traditional System ◽  
Unani Medicine ◽  
Oxidative Stresses ◽  
Capparis Decidua

Nano particles have an enormous impact on society. Selenium nanoparticles are used in various oxidative stresses. Capparis decidua is a plant which belongs to a family Capparidaceae. Capparis decidua is found in desert and semi desert areas and is used in Unani medicine and traditional system of medicine. The aim of the present study was to evaluate the antibacterial activity of selenium nanoparticles synthesized using Capparis decidua. Antibacterial activity was studied by inhibition zone against E.coli and Lactobacillus using Agar well diffusion method which was characterized by a clear zone. Selenium nanoparticles extracted from Capparis decidua fruit showed good antibacterial activity against lactobacillus species and E.coli.

scholarly journals EFFECT OF GARLIC POWDER ADDITION TO GELATIN BIOCOMPOSITE ON ITS ANTIBACTERIAL ACTIVITY

2016 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
Pramudita Putri Kusuma ◽  
Ganjar Fadillah ◽  
Husna Syaima ◽  
Teguh Endah Saraswati
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Weight Ratio ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Powder Preparation ◽  
Natural Preservative ◽  
Garlic Powder ◽  
Optimum Diameter ◽  
Powder Addition

<p>The addition of garlic powder to gelatin from chicken claw waste was potentially developed as a natural preservative in food, especially for meat. Preparation of gelatin/garlic biocomposite was performed in three stages: synthesis of gelatin from chicken claw, garlic powder preparation as allicin source and preparation of biocomposite gelatin/garlic. The preparation of dry biocomposites was done by weighing the gelatin and garlic powder in weight ratio of 1 : 1 and 1 : 2 (w/w) in the total mass of 0.75 grams. For wet biocomposite preparation, the mixture of the powder was solved in 5 mL of lactic acid 2 %. Functional groups of gelatin, garlic and biocomposite were analyzed by <em>f</em><em>ourier transform infrared spectroscopy</em> (FTIR). The antibacterial activity of biocomposite against <em>Staphylococcus aureus</em> were tested using disc diffusion method. This test was performed on garlic powder, solvent and gelatin/garlic biocomposites powder in the ratio of 1 : 1 and 1 : 2 in 2 % lactic acid solvent. The biocomposite with a weight ratio of gelatin : garlic of  1 : 1 had the optimum diameter of inhibition zone. The effectiveness of biocomposite gelatin/garlic as natural preservative applied in meat was also physically studied by organoleptic analysis. Organoleptic analysis through the hedonic test was conducted on the parameters of color, smell, and texture of gelatin/garlic biocomposites-coated meat. The results showed that the addition of garlic can increase the effectiveness of gelatin as a natural preservative of meat for four days stored in closed packaging at room temperature.</p>

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

2020 ◽  
Vol 8 (3) ◽  
pp. 177
Author(s):  
Achmad Syarifudin Noor ◽  
Bawon Triatmoko ◽  
Nuri Nuri
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction ◽  
Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

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