scholarly journals Effect of Different Diets on the Hepatopancreatic Proteomes of Chinese Mitten Crab (Eriocheir Sinensis)

2020 ◽  
Author(s):  
Xiaowen Chen ◽  
Xin Hou ◽  
Zhihua Li ◽  
Deyin Lu ◽  
Jun Wang ◽  
...  
Keyword(s):  
Aquatic Plants ◽  
Growth And Development ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Food Sources ◽  
Natural Food ◽  
Freshwater Snails ◽  
Mitten Crab ◽  
Group A ◽  
Ecological Culture

Abstract Aquatic plants and freshwater snails are important natural food sources of Eriocheir sinensis. The effects of these two kinds of natural food sources on the growth and development of Eriocheir sinensis were studied by determining the hepatopancreatic proteomes of three crab groups, namely, crabs fed with aquatic plants combined with freshwater snails (group A), crabs fed with aquatic plants only (group B), and crabs fed with freshwater snails only (group C), with tandem mass tag technology. Results showed 110 differentially expressed proteins between groups A and B, among which 78 were up-regulated and 32 were down-regulated in group A. Meanwhile, 9 proteins were up-regulated and 14 proteins were down-regulated in group A relative to those in group C. The proteins related to molting and growth that were differentially expressed between groups A and B were up-regulated in group A. These proteins included cryptocyanin and cuticle protein CBM. The immunity-related proteins, such as mannosyl-oligosaccharide glucosidase and glutathione peroxidase, that were differentially expressed between groups A and C and were up-regulated in group A. These results indicated that freshwater snails might promote the growth and development of E. sinensis to a certain extent, and aquatic plants might play an important role in the immunity of E. sinensis. Our study provides a theoretical basis for the practice of “planting grass and throwing snails” in the green ecological culture of E. sinensis.

scholarly journals Effect of different diets on the hepatopancreatic proteomes of Chinese mitten crab, Eriocheir sinensis

2021 ◽  
Author(s):  
Xiaowen Chen ◽  
Xin Hou ◽  
Zhihua Li ◽  
Deyin Lu ◽  
Jun Wang ◽  
...  
Keyword(s):  
Aquatic Plants ◽  
Growth And Development ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Food Sources ◽  
Natural Food ◽  
Freshwater Snails ◽  
Mitten Crab ◽  
Group A ◽  
Group B

Abstract Background: Aquatic plants and freshwater snails are important natural food sources of Eriocheir sinensis, which play important roles in the growth and development of E. sinensis. However, research on how aquatic plants and freshwater snails affect the growth and development of E. sinensis remains scant.Methods: The effects of the two kinds of natural food sources on the growth and development of E. sinensis were studied by determining the hepatopancreatic proteomes of three groups, namely, E. sinensis fed with aquatic plants combined with freshwater snails (group A), E. sinensis fed with aquatic plants only (group B), and E. sinensis fed with freshwater snails only (group C), using tandem mass tag technology.Results: A total of 110 differentially expressed proteins between groups A and B were identified, among which 78 were up-regulated and 32 were down-regulated in group A. Meanwhile, nine proteins were up-regulated, and 14 proteins were down-regulated in group A relative to those in group C. The proteins related to molting and growth such as cryptocyanin and cuticle protein CBM were up-regulated in group A compared with group B. The immunity-related proteins, such as mannosyl-oligosaccharide glucosidase and glutathione peroxidase, that were differentially expressed between groups A and C, were up-regulated in group A.Conclusion: The results indicated that freshwater snails might promote the growth and development of E. sinensis to a certain extent, and aquatic plants might play an important role in the immunity of E. sinensis. Our study provides a theoretical basis for the practice of “providing aquatic plants and freshwater snails” in the ecological culture of E. sinensis.

scholarly journals Plant and Animal-Type Feedstuff Shape the Gut Microbiota and Metabolic Processes of the Chinese Mitten Crab Eriocheir sinensis

2021 ◽  
Vol 8 ◽  
Author(s):  
Xiaowen Chen ◽  
Deyin Lu ◽  
Zhihua Li ◽  
Wucheng Yue ◽  
Jun Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Aquatic Plants ◽  
Gut Microbiome ◽  
Eriocheir Sinensis ◽  
Freshwater Snails ◽  
Rrna Gene ◽  
Metabolic Profiles ◽  
Metabolic Processes ◽  
Mitten Crab

In animals, growth and development are strongly correlated with the gut microbiota and metabolic profiles. In this study, gut microbiome communities, metabolic profiles, and growth performance of Eriocheir sinensis under three dietary feed types based on waterweed plants only, freshwater snails only, and waterweed plants combined with freshwater snails were studied by using 16S rRNA gene sequencing and liquid chromatography-mass spectrometry. Results indicated that different feed types dramatically affected the growth performances of E. sinensis by altering the gut microbiota and metabolic profiles. Aquatic plants, such as waterweeds, played essential roles in shaping gut microbiome communities, and the optimal Bacteroides-to-Firmicutes ratio might strongly promote growth performance. Waterweed plants also helped decrease maleficent Proteobacteria caused by excess animal-type feedstuff, such as freshwater snails, and might have positive roles in antibacterial functions in gut. A diet based on waterweeds only resulted in lipid metabolism disorders, which significantly retarded the growth of E. sinensis. In summary, E. sinensis cultured with a diet of waterweeds and freshwater snails showed superior growth performance due to their healthy gut microbiota and metabolic homeostasis. Our findings unveiled the roles of aquatic plants and animal-type food such as freshwater snail in shaping the gut microbiota and metabolic processes and provided guidance for the aquaculture of E. sinensis in future.

scholarly journals Proteomic Analysis of the Hemolymph After Metschnikowia bicuspidata Infection in the Chinese Mitten Crab Eriocheir sinensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongbo Jiang ◽  
Jie Bao ◽  
Yuenan Xing ◽  
Chengcheng Feng ◽  
Xiaodong Li ◽  
...  
Keyword(s):  
Serine Protease ◽  
Light Chain ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Related Factor ◽  
Differentially Expressed Proteins ◽  
Chinese Mitten Crab ◽  
Monitoring Method ◽  
Elastase Inhibitor ◽  
Mitten Crab

The “milky disease” of the Chinese mitten crab, Eriocheir sinensis, is a highly lethal fungal disease caused by Metschnikowia bicuspidata infection. To elucidate the immune responses of the hemolymph of E. sinensis to M. bicuspidata infection, a comparative analysis of the hemolymph of E. sinensis infected with M. bicuspidata and that treated with phosphate buffered saline was performed using label-free quantitative proteomics. A total of 429 proteins were identified. Using a 1.5-fold change in expression as a physiologically significant benchmark, 62 differentially expressed proteins were identified, of which 38 were significantly upregulated and 24 were significantly downregulated. The upregulated proteins mainly included cytoskeleton-related proteins (myosin regulatory light chain 2, myosin light chain alkali, tubulin α-2 chain, and tubulin β-1 chain), serine protease and serine protease inhibitor (clip domain-containing serine protease, leukocyte elastase inhibitor, serine protein inhibitor 42Dd), catalase, transferrin, and heat shock protein 70. Upregulation of these proteins indicated that phenoloxidase system, phagocytosis and the ROS systems were induced by M. bicuspidata. The downregulated proteins were mainly organ and tissue regeneration proteins (PDGF/VEGF-related factor protein, integrin-linked protein kinase homing pat-4 gene) and hemagglutination-associated proteins (hemolymph clottable protein, hemocyte protein-glutamine gamma-glutamyltransferase). Downregulation of these proteins indicated that M. bicuspidata inhibited hemocyte regeneration and hemolymph agglutination. Fifteen differentially expressed proteins related to immunity were verified using a parallel reaction monitoring method. The expression trend of these proteins was similar to that of the proteome. To the best of our knowledge, this is the first report on the proteome of E. sinensis in response to M. bicuspidata infection. These results not only provide new and important information on the immune response of crustaceans to yeast infection but also provide a basis for further understanding the molecular mechanism of complex host pathogen interactions between crustaceans and fungi.

Transcriptome sequencing revealed the genes and pathways involved in salinity stress of Chinese mitten crab, Eriocheir sinensis

2014 ◽  
Vol 46 (5) ◽  
pp. 177-190 ◽  
Author(s):  
Erchao Li ◽  
Shaolin Wang ◽  
Chao Li ◽  
Xiaodan Wang ◽  
Ke Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Salinity Stress ◽  
Transcriptome Sequencing ◽  
Muscle Protein ◽  
Expression Patterns ◽  
Eriocheir Sinensis ◽  
Differentially Expressed ◽  
Control Group ◽  
Protein Ubiquitination ◽  
Mitten Crab

A total of 276.9 million reads were obtained and assembled into 206, 371 contigs with an average length of 614 bp and N50 of 1,470 bp. Comparison of digital gene expression between treatment and control group reveals 1,151 and 941 genes were significantly differentially expressed in crab gill and muscle, respectively. In gill and muscle, protein ubiquitination, ubiquinone biosynthesis, oxidative phosphorylation, and mitochondria dysfunction pathways were the top pathways differentially expressed following the challenge. EIF 2 signaling pathway and IGF-1 signaling pathway were the top ones among the signal-related pathways. Most of the amino acid metabolism pathways were found to be involved in this process. The expression patterns of 15 differentially expressed genes were validated by quantitative real-time RT-PCR (average correlation coefficient 0.80). This is the first report of expression analysis of genes and pathways involved in osmoregulation of Eriocheir sinensis through transcriptome sequencing. The findings of this study will further promote the understanding of the underlying molecular mechanism of salinity stress adaptation for crustacean species.

scholarly journals Transcriptome Analysis of Differentially Expressed Genes Related to the Growth and Development of the Jinghai Yellow Chicken

Genes ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 539 ◽  
Author(s):  
Fuxiang Chen ◽  
Pengfei Wu ◽  
Manman Shen ◽  
Mingliang He ◽  
Lan Chen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Differentially Expressed Genes ◽  
Growth And Development ◽  
Slow Growth ◽  
Growth Traits ◽  
Fast Growth ◽  
Differentially Expressed ◽  
Group A ◽  
Upregulated Genes ◽  
Jinghai Yellow Chicken

The growth traits are important traits in chickens. Compared to white feather broiler breeds, Chinese local broiler breeds have a slow growth rate. The main genes affecting the growth traits of local chickens in China are still unclear and need to be further explored. This experiment used fast-growth and slow-growth groups of the Jinghai Yellow chicken as the research objects. Three males and three females with similar body weights were selected from the two groups at four weeks old and eight weeks old, respectively, with a total of 24 individuals selected. After slaughter, their chest muscles were taken for transcriptome sequencing. In the differentially expressed genes screening, all of the genes obtained were screened by fold change ≥ 2 and false discovery rate (FDR) < 0.05. For four-week-old chickens, a total of 172 differentially expressed genes were screened in males, where there were 68 upregulated genes and 104 downregulated genes in the fast-growth group when compared with the slow-growth group. A total of 31 differentially expressed genes were screened in females, where there were 11 upregulated genes and 20 downregulated genes in the fast-growth group when compared with the slow-growth group. For eight-week-old chickens, a total of 37 differentially expressed genes were screened in males. The fast-growth group had 28 upregulated genes and 9 downregulated genes when compared with the slow-growth group. A total of 44 differentially expressed genes were screened in females. The fast-growth group had 13 upregulated genes and 31 downregulated genes when compared with the slow-growth group. Through gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, many genes were found to be related to cell proliferation and differentiation, muscle growth, and cell division such as SNCG, MCL1, ARNTL, PLPPR4, VAMP1, etc. Real-time PCR results were consistent with the RNA-Seq data and validated the findings. The results of this study will help to understand the regulation mechanism of the growth and development of Jinghai Yellow chicken and provide a theoretical basis for improving the growth rate of Chinese local chicken breeds.

scholarly journals The Innate Immune Response to Infection by Polyascus gregaria in the Male Chinese Mitten Crab (Eriocheir sinensis), Revealed by Proteomic Analysis

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 57
Author(s):  
Yanping Yang ◽  
Fengjiao Ma ◽  
Juanjuan Dong ◽  
Lianxing Li ◽  
Ping Ren ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Protein Level ◽  
Eriocheir Sinensis ◽  
Innate Immune ◽  
Differentially Expressed ◽  
Economic Losses ◽  
Chinese Mitten Crab ◽  
Kegg Pathways ◽  
Mitten Crab

The Chinese mitten crab (Eriocheir sinensis) is a representative catadromous invertebrate of the Yangtze River and a commercial species widely cultivated in China. Both cultivated and wild crabs suffer from a variety of parasites and pathogens, which can result in catastrophic economic losses in aquaculture revenue. Polyascus gregaria, a parasitic barnacle with a highly derived morphology, is specialized in invading these crabs. This study examines the immunological mechanism in E. sinensis infected with P. gregaria. Tandem mass tags (TMT), a specialized method of mass-spectrometry, was used to analyze the infection by P. gregaria resistance at the protein level. In the hepatopancreas of infected crabs, 598 proteins differentially expressed relating to physiological change, of which, 352 were upregulated and 246 were downregulated. Based on this differential protein expression, 104 GO terms and 13 KEGG pathways were significantly enriched. Differentially expressed proteins, such as ATG, cathepsin, serpin, iron-related protein, Rab family, integrin, and lectin, are associated with the lysosome GO term and the autophagy-animal KEGG pathways, both of which likely relate to the immune response to the parasitic P. gregaria infection. These results show the benefit of taking a detailed, protein-level approach to understanding the innate immune response of aquatic invertebrates to macroparasite infection.

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