scholarly journals Plasmid-Mediated Resistance to Extended-Spectrum Cephalosporins and Resistance to Fluoroquinolones in Escherichia Coli Isolates from Black-headed Gulls (Larus Ridibundus)

2021 ◽  
Author(s):  
Maja Velhner ◽  
Dalibor Todorović ◽  
Katarina Novović ◽  
Branko Jovčić ◽  
Gospava Lazić ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
Extended Spectrum ◽  
Cephalosporin Antibiotics ◽  
High Level ◽  
Group D ◽  
Sequence Types ◽  
Replicon Type ◽  
Level Resistance ◽  
Game Animals

Abstract Although resistance to fluoroquinolones is common in E. coli isolates from farm and game animals in Serbia, currently no data are accessible on the occurrence of antibacterial resistances in E. coli isolates from gulls. Therefore, 45 cloacal swabs and 50 fecal samples from black-headed gulls were investigated for the presence of Escherichia coli isolates resistant to antibiotics. Multidrug resistance was detected in 22 E. coli isolates. High level resistance to fluoroquinolones was found in ten isolates with MIC values of ciprofloxacin ranging from 4 to 32 mg/L. Genotyping revealed single or double mutations in the quinolone resistance determining region (QRDR) of the gyrA or gyrA, parC and parE genes, respectively. Ten isolates showed resistance to extended-spectrum cephalosporin antibiotics. These ten isolates belonged to phylogenetic group B2 (five isolates), group D (four isolates) and group B1 (one isolate). An extended-spectrum β-lactamase resistance phenotype was detected in one isolate which carried the blaCTX-M-1 gene on a plasmid of the I2/FIB replicon type. Nine isolates carried blaCMY-2 genes, which were detected on conjugative plasmids in seven isolates. One transconjugant also carried hly, iroN, iss, ompT and cvaC virulence genes on the plasmid. Five different sequence types (ST38, ST2307, ST224, ST162 and ST34) were detected in E. coli isolates with ESBL or AmpC phenotype and genotype.

scholarly journals Complete Nucleotide Sequence of Plasmid pTN48, Encoding the CTX-M-14 Extended-Spectrum β-Lactamase from anEscherichia coliO102-ST405 Strain

2010 ◽  
Vol 55 (3) ◽  
pp. 1270-1273 ◽  
Author(s):  
Typhaine Billard-Pomares ◽  
Olivier Tenaillon ◽  
Hervé Le Nagard ◽  
Zoé Rouy ◽  
Stéphane Cruveiller ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Phylogenetic Group ◽  
E Coli ◽  
Extended Spectrum ◽  
Group D ◽  
Replicon Type ◽  
M 14

ABSTRACTThe sequence of pTN48, a plasmid of the FII-FIB replicon type that encodes a CTX-M-14 enzyme in anEscherichia colistrain of the phylogenetic group D2O102-ST405 clone, was determined. pTN48 is, for the most part, a mosaic of virulence, antibiotic resistance, and addiction system modules found in various other plasmids. The presence of multiple addiction systems indicates that the plasmid should be stably maintained in theE. coliclone, favoring dissemination of the CTX-M-14 enzyme.

Prevalence of extended-spectrum β-lactamase-producing Escherichia coli and residual antimicrobials in the environment in Vietnam

2017 ◽  
Vol 18 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Shinji Yamasaki ◽  
Tuyen Danh Le ◽  
Mai Quang Vien ◽  
Chinh Van Dang ◽  
Yoshimasa Yamamoto
Keyword(s):  
Escherichia Coli ◽  
Critical Role ◽  
Environmental Water ◽  
Resistant Bacteria ◽  
Human Beings ◽  
E Coli ◽  
Extended Spectrum ◽  
High Prevalence ◽  
Food Animals ◽  
High Level

AbstractEmergence and spread of antimicrobial-resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, have become serious problems worldwide. Recent studies conducted in Vietnam revealed that ESBL-producing E. coli are widely distributed in food animals and people. CTX-M-9 and CTX-M-1 are the most prevalent β-lactamases among the identified ESBLs. Furthermore, most of the ESBL-producing E. coli isolates were multi-drug resistant. Residual antimicrobials such as sulfamethoxazole, trimethoprim, sulfadimidine, cephalexin, and sulfadiazine were also detected at a high level in both animal meats and environmental water collected from several cities, including Ho Chi Minh city and Can Tho city. These recent studies indicated that improper use of antimicrobials in animal-originated food production might contribute to the emergence and high prevalence of ESBL-producing E. coli in Vietnam. Although clonal ESBL-producing E. coli was not identified, CTX-M-55 gene-carrying plasmids with similar sizes (105–139 kb) have been commonly detected in the ESBL-producing E. coli strains isolated from various food animals and human beings. This finding strongly suggests that horizontal transfer of the CTX-M plasmid among various E. coli strains played a critical role in the emergence and high prevalence of ESBL-producing E. coli in Vietnam.

scholarly journals Assessment of Shiga Toxin-Producing Escherichia coli Isolates from Wildlife Meat as Potential Pathogens for Humans

2009 ◽  
Vol 75 (20) ◽  
pp. 6462-6470 ◽  
Author(s):  
Angelika Miko ◽  
Karin Pries ◽  
Sabine Haby ◽  
Katja Steege ◽  
Nadine Albrecht ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Farm Animals ◽  
Clonal Lineage ◽  
E Coli ◽  
Virulence Markers ◽  
Potential Source ◽  
Human Illness ◽  
High Level ◽  
Game Animals

ABSTRACT A total of 140 Shiga toxin-producing Escherichia coli (STEC) strains from wildlife meat (deer, wild boar, and hare) isolated in Germany between 1998 and 2006 were characterized with respect to their serotypes and virulence markers associated with human pathogenicity. The strains grouped into 38 serotypes, but eight O groups (21, 146, 128, 113, 22, 88, 6, and 91) and four H types (21, 28, 2, and 8) accounted for 71.4% and 75.7% of all STEC strains from game, respectively. Eighteen of the serotypes, including enterohemorrhagic E. coli (EHEC) O26:[H11] and O103:H2, were previously found to be associated with human illness. Genes linked to high-level virulence for humans (stx 2, stx 2d, and eae) were present in 46 (32.8%) STEC strains from game. Fifty-four STEC isolates from game belonged to serotypes which are frequently found in human patients (O103:H2, O26:H11, O113:H21, O91:H21, O128:H2, O146:H21, and O146:H28). These 54 STEC isolates were compared with 101 STEC isolates belonging to the same serotypes isolated from farm animals, from their food products, and from human patients. Within a given serotype, most STEC strains were similar with respect to their stx genotypes and other virulence attributes, regardless of origin. The 155 STEC strains were analyzed for genetic similarity by XbaI pulsed-field gel electrophoresis. O103:H2, O26:H11, O113:H21, O128:H2, and O146:H28 STEC isolates from game were 85 to 100% similar to STEC isolates of the same strains from human patients. By multilocus sequence typing, game EHEC O103:H2 strains were attributed to a clonal lineage associated with hemorrhagic diseases in humans. The results from our study indicate that game animals represent a reservoir for and a potential source of human pathogenic STEC and EHEC strains.

scholarly journals Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria

2014 ◽  
Vol 58 (4) ◽  
pp. 2472-2474 ◽  
Author(s):  
Laurent Poirel ◽  
Encho Savov ◽  
Arzu Nazli ◽  
Angelina Trifonova ◽  
Iva Todorova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
The World ◽  
High Level ◽  
16S Rrna Methylase ◽  
Level Resistance

ABSTRACTTwelve consecutive carbapenem-resistantEscherichia coliisolates were recovered from patients (infection or colonization) hospitalized between March and September 2012 in different units at a hospital in Bulgaria. They all produced the carbapenemase NDM-1 and the extended-spectrum-β-lactamase CTX-M-15, together with the 16S rRNA methylase RmtB, conferring high-level resistance to all aminoglycosides. All those isolates were clonally related and belonged to the same sequence type, ST101. In addition to being the first to identify NDM-producing isolates in Bulgaria, this is the very first study reporting an outbreak of NDM-1-producingE. coliin the world.

scholarly journals Longitudinal Sampling Reveals Persistence of and Genetic Diversity in Extended-Spectrum Cephalosporin-Resistant Escherichia coli From Norwegian Broiler Production

2021 ◽  
Vol 12 ◽  
Author(s):  
Solveig Sølverød Mo ◽  
Madelaine Norström ◽  
Jannice Schau Slettemeås ◽  
Anne Margrete Urdahl ◽  
Amar Anandrao Telke ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Sampling Period ◽  
E Coli ◽  
Broiler Production ◽  
Extended Spectrum ◽  
Oxford Nanopore ◽  
Longitudinal Sampling ◽  
Sequence Types

There are knowledge gaps concerning dynamics of extended-spectrum cephalosporin (ESC)-resistant Escherichia coli and their plasmids in broiler production and the persistence of strains on broiler farms. Thus, we aimed at characterising ESC-resistant Escherichia coli collected from all flocks reared on 10 different farms during a six-months sampling period. All isolates (n = 43) were subjected to whole-genome sequencing, and a subset of isolates (n = 7) were also sequenced using oxford nanopore technology and subsequent hybrid assembly in order to do in-depth characterisation of the ESC resistance plasmids. The 43 isolates belonged to 11 different sequence types, and three different ESC resistance gene/plasmid combinations were present, namely, IncK2/blaCMY-2 (n = 29), IncI1/blaCMY-2 (n = 6) and IncI1/blaCTX-M-1 (n = 8). ESC-resistant E. coli of different STs and with different ESC resistance gene/plasmid combinations could be present on the same farm, while a single ST and ESC resistance gene/plasmid displaying zero or few SNP differences were present on other farms. In-depth characterisation of IncK2/blaCMY-2 plasmids revealed that at least two distinct variants circulate in the broiler production. These plasmids showed close homology to previously published plasmids from other countries. Our longitudinal study show that ESC-resistant E. coli belong to a multitude of different STs and that different ESC resistance genes and plasmids occur. However, there is also indication of persistence of both ESC-resistant E. coli strains and IncK2/blaCMY-2 plasmids on farms. Further studies are warranted to determine the dynamics of strains, plasmids and ESC resistance genes within single broiler flocks.

scholarly journals Emergence of Escherichia coli Sequence Type 131 Isolates Producing KPC-2 Carbapenemase in China

2013 ◽  
Vol 58 (2) ◽  
pp. 1146-1152 ◽  
Author(s):  
Jia Chang Cai ◽  
Rong Zhang ◽  
Yan Yan Hu ◽  
Hong Wei Zhou ◽  
Gong-Xiang Chen
Keyword(s):  
Escherichia Coli ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Predominant Type ◽  
Group A ◽  
Dna Fragment ◽  
High Level ◽  
Group D

ABSTRACTTwenty-two KPC-2-producingEscherichia coliisolates were obtained from three hospitals in Hangzhou, China, from 2007 to 2011. One isolate, with OmpC porin deficiency, exhibited high-level carbapenem resistance. Pulsed-field gel electrophoresis showed that few isolates were indistinguishable or closely related. Multilocus sequence typing indicated that sequence type 131 (ST131) was the predominant type (9 isolates, 40.9%), followed by ST648 (5 isolates), ST405 (2 isolates), ST38 (2 isolates), and 4 single STs, ST69, ST2003, ST2179, and ST744. Phylogenetic analysis indicated that 9 group B2 isolates belonged to ST131, and 5 of 11 group D isolates belonged to ST648. Only one group B1 isolate and one group A isolate were identified. A representative plasmid (pE1) was partially sequenced, and a 7,788-bp DNA fragment encoding Tn3transposase, Tn3resolvase, ISKpn8transposase, KPC-2, and ISKpn6-like transposase was obtained. TheblaKPC-2-surrounding sequence was amplified by a series of primers. The PCR results showed that 13 isolates were consistent with the genetic environment in pE1. It is the first report of rapid emergence of KPC-2-producingE. coliST131 in China. TheblaKPC-2gene of most isolates was located on a similar genetic structure.

scholarly journals Human isolates of apramycin-resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme

1993 ◽  
Vol 110 (2) ◽  
pp. 253-259 ◽  
Author(s):  
J. E. B. Hunter ◽  
C. A. Hart ◽  
J. C. Shelley ◽  
J. R. Walton ◽  
M. Bennett
Keyword(s):  
Escherichia Coli ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Aminoglycoside Antibiotic ◽  
Dna Probe ◽  
Conjugative Plasmid ◽  
E Coli ◽  
High Level ◽  
Level Resistance

SUMMARYGentamicin-resistant Escherichia coli isolated at different periods from patients in two hospitals were tested for resistance to the aminoglycoside antibiotic apramycin. Twenty-four of 93 (26%) gentamicin-resistant isolates collected from the Royal Liverpool Hospital between 1981 and 1990 were resistant to apramycin. Thirteen isolates were highly resistant to apramycin (minimal inhibitory concentration (MIC) ≥ 1024 μg/ml). were also resistant to gentamicin, netilmicin and tobramycin, and hybridized with a DNA probe derived from the aminoglycoside acetyltransferase (3)IV (AAC(3)IV) gene. The proportion of gentamicinresistant isolates which had high level resistance to apramycin increased from 7% in 1981–5 to 24% in 1986–90.Twelve gentamicin-resistant E. coli from Guy's and St Thomas's Hospital isolated between 1977 and 1980 were also tested for resistance to apramycin. For five of these isolates the MICs of apramycin was 32–256 μg/ml. None was shown to have a conjugative plasmid carrying resistance to apramycin and only one hybridized with the DNA probe for the AAC(3)IV enzyme.

scholarly journals Molecular Epidemiology of Fosfomycin Resistant E. coli from a Pigeon Farm in China

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 777
Author(s):  
Lu Han ◽  
Xiao-Qing Lu ◽  
Xu-Wei Liu ◽  
Mei-Na Liao ◽  
Ruan-Yang Sun ◽  
...  
Keyword(s):  
Molecular Characteristics ◽  
Genomic Context ◽  
E Coli ◽  
High Prevalence ◽  
Potential Reservoir ◽  
Genomic Environment ◽  
High Level ◽  
Human Infections ◽  
Sequence Types ◽  
Level Resistance

We determined the prevalence and molecular characteristics of fosfomycin-resistant Escherichia coli from a domestic pigeon farm. A total of 79 samples collected from pigeons and their surrounding environments were screened for the presence of fosfomycin resistant isolates and these included 49 E. coli isolates that displayed high-level resistance (MIC ≥ 256 mg L−1) and carried the fosA3 gene on plasmids with sizes ranging from 80 to 370 kb. MLST analysis of these fosA3-positive E. coli isolates indicated the presence of nine sequence types (ST6856, ST8804, ST457, ST746, ST533, ST165, ST2614, ST362 and ST8805) of which ST6856 was the most prevalent (24.5%, 12/49). PFGE combined with genomic context comparative analyses indicated that the fosA3 gene was spread by horizontal transfer as well as via clonal transmission between E. coli in the pigeon farm, and IS26 played an important role in fosA3 transmission. The high prevalence of fosA3 in the pigeon farm and the high similarity of the fosA3 genomic environment between E. coli isolates from humans and pigeons indicated that the pigeon farm served as a potential reservoir for human infections. The pigeon farm was found to be an important reservoir for the fosA3 gene and this should be further monitored.

scholarly journals Identification of FosA8, a Plasmid-Encoded Fosfomycin Resistance Determinant from Escherichia coli, and Its Origin in Leclercia adecarboxylata

2019 ◽  
Vol 63 (11) ◽  
Author(s):  
Laurent Poirel ◽  
Xavier Vuillemin ◽  
Nicolas Kieffer ◽  
Linda Mueller ◽  
Marie-Christine Descombes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid Identity ◽  
Natural Resistance ◽  
Whole Genome ◽  
Content Type ◽  
Acid Identity ◽  
E Coli ◽  
Fosfomycin Resistance ◽  
High Level ◽  
Level Resistance

ABSTRACT A plasmid-located fosfomycin resistance gene, fosA8, was identified from a CTX-M-15-producing Escherichia coli isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The fosA8 gene was located on a transferable 50-kb plasmid of IncN type encoding high-level resistance to fosfomycin. In silico analysis and cloning experiments identified fosA8 analogues (99% identity) in the genome of Leclercia decarboxylata, which is an enterobacterial species with natural resistance to fosfomycin. This finding adds L. decarboxylata to the list of enterobacterial species that are a reservoir of fosA-like genes which have been captured from the chromosome of a progenitor and are then acquired by E. coli.

scholarly journals Emergence of Metallo-β-Lactamase NDM-1-Producing Multidrug-Resistant Escherichia coli in Australia

2010 ◽  
Vol 54 (11) ◽  
pp. 4914-4916 ◽  
Author(s):  
Laurent Poirel ◽  
Emilie Lagrutta ◽  
Peter Taylor ◽  
Jeanette Pham ◽  
Patrice Nordmann
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Multidrug Resistant ◽  
E Coli ◽  
Extended Spectrum ◽  
High Level ◽  
Lactamase Gene

ABSTRACT A multidrug-resistant Escherichia coli isolate recovered in Australia produced a carbapenem-hydrolyzing β-lactamase. Molecular investigations revealed the first identification of the bla NDM-1 metallo-β-lactamase gene in that country. In addition, this E. coli isolate expressed the extended-spectrum β-lactamase CTX-M-15, together with two 16S rRNA methylases, namely, ArmA and RmtB, conferring a high level of resistance to aminoglycosides.

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