scholarly journals Acute Noise Causes Down-Regulation of ECM Protein Expression in Guinea Pig Cochlea

2021 ◽  
Author(s):  
Min Shi ◽  
Lei Shi ◽  
Daxiong Ding ◽  
Yiyong Hu ◽  
Guowei Qi ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Protein Expression ◽  
Focal Adhesion ◽  
Noise Exposure ◽  
Collagen Type ◽  
Hearing Threshold ◽  
Down Regulation ◽  
Marker Proteins ◽  
Proteomics Technology ◽  
Before And After

Abstract Background: Proteomics technology reveals the marker proteins, potential pathogenesis and intervention targets after noise-induced hearing loss.To study the differences in cochlea protein expression before and after noise exposure using proteomics to reveal the pathological mechanism of noise-induced hearing loss (NIHL).Methods: A guinea pig NIHL model was established to test the ABR thresholds before and after noise exposure. The proteomics technology was used to study the mechanism of differential protein expression in the cochlea by noise stimulation.Results: The average hearing threshold of guinea pigs on the first day after noise exposure was 57.00±6.78dB SPL; the average hearing threshold on the seventh day after noise exposure was 45.83±6.07dB SPL,. The proteomics technology identified 3122 different inner ear proteins, of which six proteins related to the hearing were down-regulation: TenascinC, Collagen type XI alpha two chains, Collagen type II alpha one chain, Thrombospondin 2, Collagen type XI alpha one chain and Ribosomal protein L38, and are enriched in protein absorption, focal adhesion, and extracellular matrix receptor pathways.Conclusion: Impulse noise can affect the expression of differential proteins through focal adhesion pathways. This data can provide an experimental basis for the research on the prevention and treatment of NIHL.Trial registration: Not applicable.

scholarly journals Acute noise causes down-regulation of ECM protein expression in guinea pig cochlea

2020 ◽  
Author(s):  
Min Shi ◽  
Lei Shi ◽  
Daxiong Ding ◽  
Yiyong Hu ◽  
Guowei Qi ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Guinea Pig ◽  
Protein Expression ◽  
Focal Adhesion ◽  
Noise Exposure ◽  
Collagen Type ◽  
Hearing Threshold ◽  
Down Regulation ◽  
Proteomics Technology ◽  
Before And After

AbstractObjectiveTo study the differences in cochlea protein expression before and after noise exposure using proteomics to reveal the pathological mechanism of noise-induced hearing loss (NIHL).MethodsA guinea pig NIHL model was established to test the ABR thresholds before and after noise exposure. The proteomics technology was used to study the mechanism of differential protein expression in the cochlea by noise stimulation.ResultsThe average hearing threshold of guinea pigs on the first day after noise exposure was 57.00±6.78dB SPL, high-frequency hearing loss was more severe than low frequency; the average hearing threshold on the seventh day after noise exposure was 45.83±6.07dB SPL, and the 4k Hz hearing threshold has the best recovery. The proteomics technology identified 3122 different inner ear proteins, of which six proteins related to the hearing were down-regulation: TenascinC, Collagen type XI alpha two chains, Collagen type II alpha one chain, Thrombospondin 2, Collagen type XI alpha one chain and Ribosomal protein L38, and are enriched in protein absorption, focal adhesion, and extracellular matrix receptor pathways.ConclusionImpulse noise can affect the expression of differential proteins through focal adhesion pathways. This data can provide an experimental basis for the research on the prevention and treatment of NIHL.

Pengaruh bising lalu lintas terhadap penurunan fungsi pendengaran pada juru parkir di kota Denpasar

2013 ◽  
Vol 40 (2) ◽  
Author(s):  
Ni Ketut Susilawati ◽  
Wayan Sudana ◽  
Eka Putra Setiawan
Keyword(s):  
Hearing Impairment ◽  
Noise Exposure ◽  
Traffic Noise ◽  
Hearing Threshold ◽  
Noise Pollution ◽  
Simple Random Sampling ◽  
Cross Sectional Study ◽  
Control Group ◽  
Cross Sectional ◽  
Hearing Function

Background: Noise pollution or noise is an unwanted sound which is disturbing to human beings.However small or soft the sound, if it is undesirable it is considered as noise. Noise induced hearingloss is a sensorineural hearing loss that is commonly encountered second to presbycusis. Purpose: Toknow the effect of traffic noise exposure on hearing impairment to the employees of the Parking DistrictCompany of the Denpasar city and to improve diagnostic detection on hearing impairment caused bynoise. Method: A cross sectional study was conducted at the Parking District Company office. Thepopulations of this study were the employees of the Parking District Company. Samples of this study were the employees who were exposed to traffic noise and control samples were an employee who was unexposed. Samples were selected by simple random sampling. Results: From 40 parking attendants,27 persons (67.5%) aged above 35 years old. The parking attendants who had been working for ten to fifteen years were 36 persons (90%) and no history using ear protection when working. Seven persons(17.5%) had referred DPOAE upon examination with increase hearing threshold on audiogram result.In this study the parking attendants who had hearing deficit induced by noise were 7 persons (17.5%)and only one person (2.5%) in control group. There was a statistically significant effect of traffic noiseto hearing function deficit (p<0.05). Conclusion: Traffic noise has effect in hearing function deficit onthe parking attendants.ORLI Vol. 40 No. 2 Tahun 2010Key words: NIHL, parking attendant, audiometry, DPOAE.

scholarly journals The silencing of long non-coding RNA ANRIL suppresses invasion, and promotes apoptosis of retinoblastoma cells through the ATM-E2F1 signaling pathway

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Yang Yang ◽  
Xiao-Wei Peng
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Inhibitory Effect ◽  
Down Regulation ◽  
Reading Frame ◽  
Non Coding Rna ◽  
Retinoblastoma Cells ◽  
Human Retinoblastoma ◽  
Y79 Cells ◽  
Long Non Coding Rna

As one of the most common primary intraocular carcinomas, retinoblastoma generally stems from the inactivation of the retinoblastoma RB1 gene in retinal cells. Antisense non-coding RNA in the INK4 locus (ANRIL), a long non-coding RNA (lncRNA), has been reported to affect tumorigenesis and progression of various cancers, including gastric cancer and non-small cell lung cancer. However, limited investigations emphasized the role of ANRIL in human retinoblastoma. Hence, the current study was intended to investigate the effects of ANRIL on the proliferation, apoptosis, and invasion of retinoblastoma HXO-RB44 and Y79 cells. The lentivirus-based packaging system was designed to aid the up-regulation of ANRIL and ATM expressions or employed for the down-regulation of ANRIL in human retinoblastoma cells. Afterward, ANRIL expression, mRNA and protein expression of ATM and E2F1, and protein expression of INK4b, INK4a, alternate reading frame (ARF), p53 and retinoblastoma protein (pRB) were determined in order to elucidate the regulation effect associated with ANRIL on the ATM-E2F1 signaling pathway. In addition, cell viability, apoptosis, and invasion were detected accordingly. The results indicated that the down-regulation of ANRIL or up-regulation of ATM led to an increase in the expressions of ATM, E2F1, INK4b, INK4a, ARF, p53, and pRB. The silencing of ANRIL or up-regulation of ATM exerted an inhibitory effect on the proliferation and invasion while improving the apoptosis of HXO-RB44 and Y79 cells. In conclusion, the key observations of our study demonstrated that ANRIL depletion could act to suppress retinoblastoma progression by activating the ATM-E2F1 signaling pathway. These results provide a potentially promising basis for the targetted intervention treatment of human retinoblastoma.

Acute and specific collagen type I degradation increases diastolic and developed tension in perfused rat papillary muscle

2004 ◽  
Vol 286 (3) ◽  
pp. H889-H894 ◽  
Author(s):  
Regis R. Lamberts ◽  
Maurice J. J. M. F. Willemsen ◽  
Néstor G. Pérez ◽  
Pieter Sipkema ◽  
Nico Westerhof
Keyword(s):  
Papillary Muscle ◽  
Collagen Type ◽  
Perfusion Pressure ◽  
Collagen Type I ◽  
Type I ◽  
Collagenase Treatment ◽  
Before And After ◽  
Diastolic Coronary Flow ◽  
Rat Papillary Muscle

Collagen degradation is suggested to be responsible for long-term contractile dysfunction in different cardiomyopathies, but the effects of acute and specific collagen type I removal (main type in the heart muscle) on tension have not been studied. We determined the diastolic and developed tension length relations in isometric contracting perfused rat papillary muscles (perfusion pressure 60 cmH2O) before and after acute and specific removal of small collagen struts with the use of purified collagenase type I. At 95% of the maximal length (95% Lmax), diastolic tension increased 20.4 ± 8.1% ( P < 0.05, n = 6) and developed tension increased 15.0 ± 6.7% after collagenase treatment compared with time controls. Treatment increased the diastolic muscle diameter by 7.1 ± 3.4% at 95% Lmax, whereas the change in diameter due to contraction was not changed. Diastolic coronary flow and normalized coronary arterial flow impediment did not change after collagenase treatment. Electron microscopy revealed that the number of small collagen struts, interconnecting myocytes, and capillaries was reduced to ∼32% after treatment. We conclude that removal of the small collagen struts by acute and specific collagen type I degradation increases diastolic and developed tension in perfused papillary muscle. We suggest that diastolic tension is increased due to edema, whereas developed tension is increased because the removal of the struts poses a lower lateral load on the cardiac myocytes, allowing more myocyte thickening.

scholarly journals Audiometric notch and extended high-frequency hearing threshold shift in relation to total leisure noise exposure: An exploratory analysis

2017 ◽  
Vol 19 (91) ◽  
pp. 263 ◽  
Author(s):  
Veronika Weilnhammer ◽  
Wenjia Wei ◽  
Stefanie Heinze ◽  
DorisG Gerstner ◽  
SandraM Walser ◽  
...  
Keyword(s):  
High Frequency ◽  
Noise Exposure ◽  
Hearing Threshold ◽  
Exploratory Analysis ◽  
Threshold Shift
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