Age-related changes of neutrophil characteristics, potential of activation, DNA release and NET formation

Abstract Background: Neutrophils are a heterogeneous population of leukocytes, which can be subdivided into high and low density neutrophils (HDNs/LDNs). They are known to fight intruders with different mechanisms, including the release of neutrophil extracellular traps (NETs), which have also been associated with thrombosis. Risks of infection and thrombosis increase with age. Differences in neutrophil subpopulations and functionality have been shown in various disease states, but investigations in healthy subjects and their dependence on age are lacking. The aim of this study was to investigate age-related changes in neutrophils regarding neutrophil subpopulations, their potential of activation, DNA release and NET formation.Methods: Neutrophil subpopulations (HDNs and LDNs) were isolated from 25 healthy individuals subdivided into 3 groups (<45 years, n=8; 45-54, n=9; >54, n=8). Neutrophil characteristics, potential of activation and the ability of NET formation was investigated using flow cytometry. Externalisation of DNA was detected by a DNA release assay.Results: HDN and LDN counts did not differ between age-groups. However, with increasing age we observed a shift in neutrophil subpopulations towards a lower amount of mature LDNs, characterized by their expression of membrane receptors CD62L and CD16. Upon stimulation, neutrophils of older individuals showed significantly higher release of DNA. HDNs of younger participants increased activation markers (CD66b and CD11b) to a higher extent compared to those of older individualsConclusion: Neutrophils and their ability of activation, DNA release and NET formation change with age and this might contribute to the higher risk of infection and thrombosis at advanced age. Furthermore, these results highlight the importance and necessity of age-matching in studies that focus on neutrophil characteristics.

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Age-related changes of neutrophil characteristics, potential of activation, DNA release and NET formation

10.21203/rs.3.rs-18855/v1 ◽

2020 ◽

Author(s):

Moritz Krall ◽

Lisa-Marie Mauracher ◽

Johanna Roiss ◽

Lena Hell ◽

Anna S Ondracek ◽

...

Keyword(s):

Age Groups ◽

Membrane Receptors ◽

Lower Amount ◽

Older Individuals ◽

Activation Markers ◽

Disease States ◽

Age Related ◽

Thrombosis Risk ◽

Dna Release ◽

Age Related Changes

Abstract Background: Neutrophils are a heterogeneous population of leukocytes, which can be subdivided into high and low density neutrophils (HDNs/LDNs). They are known to fight intruders with different mechanisms, including the release of neutrophil extracellular traps (NETs), which have also been associated with thrombosis. Risk of both, thrombosis and infections, increase with age. Differences in neutrophil subpopulations and functionality have been shown in various disease states, but investigations in healthy subjects and their dependence on age are lacking. The aim of this study was to investigate age-related changes in neutrophils regarding neutrophil subpopulations, their potential of activation, DNA release and NET formation. Methods: Neutrophil subpopulations (HDNs and LDNs) were isolated from 25 healthy individuals subdivided into 3 groups (<45 years, n=8; 45-54, n=9; >54, n=8). Neutrophil characteristics, potential of activation and the ability of NET formation were investigated using flow cytometry. Externalisation of DNA was detected by a DNA release assay. Results: HDN and LDN counts did not differ between age-groups. However, with increasing age we observed a shift in neutrophil subpopulations towards a lower amount of mature LDNs, characterized by their expression of membrane receptors CD62L and CD16. Upon stimulation, neutrophils of older individuals showed significantly higher release of DNA. NET formation was associated with increasing age. HDNs of younger participants increased activation markers (CD66b and CD11b) to a higher extent compared to those of older individuals. Conclusion: Neutrophils and their ability of activation, DNA release and NET formation change with age and this might contribute to the higher risk of infection and thrombosis at advanced age. Furthermore, these results highlight the importance and necessity of age-matching in studies that focus on neutrophil characteristics.

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Age-related changes in MEG theta and alpha oscillatory signatures of attentional control

10.1101/461020 ◽

2018 ◽

Cited By ~ 1

Author(s):

Eleanor Huizeling ◽

Hongfang Wang ◽

Carol Holland ◽

Klaus Kessler

Keyword(s):

Attentional Control ◽

Age Groups ◽

Visual Presentation ◽

Theta Oscillations ◽

Older Individuals ◽

Middle Aged ◽

Attention Task ◽

Age Related ◽

Middle Aged Adults ◽

Age Related Changes

AbstractIn our recent behavioural research (Callaghan et al., 2017), we reported age-related changes in the costs of switching between from a temporal (rapid serial visual presentation) to a spatial (visual search) attention task. Using magnetoencephalography, we have now compared the neural signatures of attention refocusing between three age groups (19-30, 40-49 and 60+ years) and found differences in task-related modulation and cortical localisation of alpha and theta oscillations. Efficient, faster switching between the temporal and spatial attention tasks in the youngest group compared to both older groups was reflected in parietal theta effects that were significantly reduced in the older groups. Residual parietal theta activity in older individuals was, however, beneficial to attentional switching, and could reflect the preservation of attention mechanisms. Difficulties in refocusing attention in the older and middle-aged adults (slowed response times) were accompanied by reduced theta power modulation in occipital and cerebellar regions. In addition to this posterior theta deficit, older and middle-aged adults presented with increased recruitment of frontal (both groups) and temporal (older group) areas. Theta oscillations therefore demonstrated a posterior to anterior processing shift, which may reflect the engagement of alternative strategies in older- and middle-age, such as top-down attentional control in an attempt to compensate for posterior processing deficits. Additional frontal theta recruitment indeed appeared to be beneficial for faster performance, as reflected by correlational analysis. Temporal theta (in older-age), however, did not correlate with performance, but could reflect strategies unrelated to speeded performance (e.g. silent vocalisation to maintain task goals). Alpha oscillations did not significantly correlate with task performance, possibly reflecting decreased neural precision or de-differentiation, but require further investigation.

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Determination of age-related changes in human soluble interleukin 2 receptor in body fluids of normal subjects as a control value against disease states

Clinica Chimica Acta ◽

10.1016/s0009-8981(99)00161-8 ◽

1999 ◽

Vol 289 (1-2) ◽

pp. 89-97 ◽

Cited By ~ 11

Author(s):

Yoshimitsu Gotoh ◽

Yoshihiro Okamoto ◽

Osamu Uemura ◽

Naoko Mori ◽

Soichi Tanaka ◽

...

Keyword(s):

Interleukin 2 ◽

Normal Subjects ◽

Body Fluids ◽

Control Value ◽

Interleukin 2 Receptor ◽

Disease States ◽

Age Related ◽

Soluble Interleukin 2 Receptor ◽

Age Related Changes

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Age-related mortality in 61,993 confirmed COVID-19 cases over three epidemic waves in Aragon, Spain. Implications for vaccination programmes

PLoS ONE ◽

10.1371/journal.pone.0261061 ◽

2021 ◽

Vol 16 (12) ◽

pp. e0261061

Author(s):

Diego Casas-Deza ◽

Vanesa Bernal-Monterde ◽

Angel Nicolás Aranda-Alonso ◽

Enrique Montil-Miguel ◽

Ana Belen Julián-Gomara ◽

...

Keyword(s):

Age Groups ◽

Case Fatality ◽

The Elderly ◽

Progressive Increase ◽

University Hospital ◽

Rapid Progression ◽

Targeted Prevention ◽

Older Individuals ◽

Prevention Measures ◽

Age Related

Background Risk for severe COVID-19 increases with age. Different vaccination strategies are currently being considered, including those aimed at slowing down transmission and those aimed at providing direct protection to those most at risk. Methods The objectives of the current study were i) to assess age-related incidence and survival between PCR-diagnosed COVID-19 cases (n = 61,993) in the Autonomous Community of Aragon from March to November 2020, and ii) to characterize age differences regarding the course of the disease in hospitalized patients in a tertiary university hospital. Results We found a similar incidence of COVID-19 in individuals between 10 and 79 years. Incidence increased in those over 80 years possibly because of the elevated transmission within the nursing homes. We observed a profound disparity among age groups; case fatality rates (CFRs) were near 0 in cases younger than 39 years throughout different waves. In contrast, there was an age-dependent and progressive increase in the CFRs, especially during the first pandemic wave. SARS-CoV-2 infection caused a more severe and rapid progression in older patients. The elderly required faster hospitalization, presented more serious symptoms on admission, and had a worse clinical course. Hospitalized older individuals, even without comorbidities, had an increased mortality risk directly associated with their age. Lastly, the existence of comorbidities dramatically increased the CFRs in the elderly, especially in males. Conclusion The elevated incidence of COVID-19 and the vulnerability of the elderly call for their prioritization in vaccination and targeted prevention measures specifically focused on this aged population.

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Age-related changes in behavioural and emotional problems in Smith–Magenis syndrome measured with the Developmental Behavior Checklist

Journal of Intellectual Disabilities ◽

10.1177/1744629519901056 ◽

2020 ◽

pp. 174462951990105 ◽

Cited By ~ 1

Author(s):

Heidi Elisabeth Nag ◽

Terje Nærland

Keyword(s):

Age Groups ◽

Emotional Problems ◽

Chromosome 17 ◽

Adaptive Behaviour ◽

Genetic Syndrome ◽

Developmental Behavior ◽

Age Related ◽

Autism Symptomatology ◽

Behavioural And Emotional Problems ◽

Age Related Changes

Smith–Magenis syndrome (SMS) is a genetic syndrome most often caused by a deletion on chromosome 17 or more rarely by a mutation in the retinoic acid-induced 1 gene. The aim of this study was to investigate the Developmental Behavior Checklist (DBC) profile of persons with SMS and the associations between behavioural and emotional problems, age, gender, adaptive behaviour and autism symptomatology. Twenty-eight persons with SMS were represented by their parents in this study. DBC Total scores are reduced with age, but they still show a mean that is clearly above the cut-off of 46. The differences between the age groups <9 years and 9–17 years ( p = 0.024) and between the age groups <9 years and >18 years ( p = 0.007) are significant. We found a significant decrease in behavioural and emotional problems with age in SMS. We did not find a relationship between adapted behaviour and communication and behavioural and emotional problems.

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Automated Speech Recognition Systems and Older Adults: A Literature Review and Synthesis

Proceedings of the Human Factors and Ergonomics Society Annual Meeting ◽

10.1177/1071181319631121 ◽

2019 ◽

Vol 63 (1) ◽

pp. 42-46 ◽

Cited By ~ 1

Author(s):

Lauren Werner ◽

Gaojian Huang ◽

Brandon J. Pitts

Keyword(s):

Older Adults ◽

Speech Recognition ◽

Current Knowledge ◽

Age Groups ◽

Older Individuals ◽

Age Related ◽

Wide Range ◽

Automated Speech Recognition ◽

Technological Developments ◽

Physical Changes

The number of older adults is growing significantly worldwide. At the same time, technological developments are rapidly evolving, and older populations are expected to interact more frequently with such sophisticated systems. Automated speech recognition (ASR) systems is an example of one technology that is increasingly present in daily life. However, age-related physical changes may alter speech production and limit the effectiveness of ASR systems for older individuals. The goal of this paper was to summarize the current knowledge on ASR systems and older adults. The PRISMA method was employed and 17 studies were compared on the basis of word error rate (WER). Overall, WER was found to be influenced by age, gender, and the number of speech samples used to train ASR systems. This work has implications for the development of future human-machine technologies that will be used by a wide range of age groups.

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Investigation of Age-Related Changes in the Skin Microbiota of Korean Women

Microorganisms ◽

10.3390/microorganisms8101581 ◽

2020 ◽

Vol 8 (10) ◽

pp. 1581

Author(s):

Minseok Kim ◽

Tansol Park ◽

Jung Im Yun ◽

Hye Won Lim ◽

Na Rae Han ◽

...

Keyword(s):

Age Groups ◽

Alpha Diversity ◽

Amplicon Sequencing ◽

Diversity Indices ◽

Rrna Gene ◽

Korean Women ◽

Skin Microbiota ◽

Microbial Distribution ◽

Age Related ◽

Age Related Changes

The microbiota of human skin is influenced by host and environmental factors. To determine if chronological age influences the composition of the skin microbiota on the forehead and hands, 73 Korean women were sorted into one of three age groups: (1) 10–29 years (n = 24), (2) 30–49 years (n = 21), and (3) 50–79 years (n = 28). From the 73 women, 146 skin samples (two skin sites per person) were collected. 16S rRNA gene amplicon sequencing was then conducted to analyze the skin microbiota. The overall microbial distribution varied on the forehead but was similar on the hands across the three age groups. In addition, the composition of the skin microbiota differed between the forehead and hands. Commensal microbiota, such as Streptococcus, Staphylococcus, Cutibacterium, and Corynebacterium, which contribute to maintaining skin health via dominant occupation, were affected by increasing age on forehead and hand skin. Alpha diversity indices increased significantly with age on forehead skin. This study indicates that older people may be more susceptible to pathogenic invasions due to an imbalanced skin microbiota resulting from age-related changes. The results of our study may help develop new strategies to rebalance skin microbiota shifted during aging.

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Age Related Changes in Weight of the Thymus Gland of Bangladeshi People

Bangladesh Journal of Anatomy ◽

10.3329/bja.v8i1.6101 ◽

1970 ◽

Vol 8 (1) ◽

pp. 10-12

Author(s):

Meherunnessa Begum ◽

Uttam Kumar Paul ◽

Md Jahangir Alam

Keyword(s):

Effect of aging on tracheal mucociliary clearance in beagle dogs

Journal of Applied Physiology ◽

10.1152/jappl.1987.62.3.1331 ◽

1987 ◽

Vol 62 (3) ◽

pp. 1331-1334 ◽

Cited By ~ 19

Author(s):

S. L. Whaley ◽

B. A. Muggenburg ◽

F. A. Seiler ◽

R. K. Wolff

Keyword(s):

Young Adult ◽

Gamma Camera ◽

Mucociliary Clearance ◽

Age Groups ◽

Beagle Dogs ◽

Velocity Measurements ◽

Middle Aged ◽

Age Related ◽

Age Related Changes ◽

Made In

Tracheal mucous velocity measurements were made in 24 beagle dogs in five age groups, using a gamma camera to detect movement of instilled 99mTc-macroaggregated albumin. Age groups were defined as immature (9–10 mo), young adult (2.8–3.0 yr), middle aged (6.7–6.9 yr), mature (9.6–9.8 yr), and aged dogs (13.6–16.2 yr). Mean velocities were 3.6 +/- 0.4 (SE) mm/min in the immature dogs, 9.7 +/- 0.6 mm/min in the young adults, 6.9 +/- 0.5 mm/min in the middle-aged dogs, 3.5 +/- 0.8 mm/min in the mature dogs, and 2.9 +/- 0.5 mm/min in the aged dogs. Tracheal mucous velocity was significantly (P less than 0.05) greater in the young adult and middle-aged groups compared with the immature, mature, and aged dogs. This pattern of age-related changes was noted to be similar to age-related changes described for certain pulmonary function measurements.

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