Monitoring Urinary Collagen Metabolite Changes Following Collagen Peptide Ingestion and Physical Activity Using ELISA With Anti–Active-Collagen-Oligopeptide Antibody

Abstract Active collagen oligopeptides are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC-MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.

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Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody

Scientific Reports ◽

10.1038/s41598-021-92934-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yoshihiro Osawa ◽

Kaho Nomura ◽

Yoshifumi Kimira ◽

Seiji Kushibe ◽

Ken-ichi Takeyama ◽

...

Keyword(s):

Physical Activity ◽

Student Athletes ◽

Cross Reactivity ◽

University Student ◽

Collagen Metabolism ◽

The Novel ◽

Urinary Level ◽

Collagen Peptide ◽

Urinary Levels ◽

Over Time

AbstractActive collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.

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Physical activity in university student athletes, prior and in confinement due to pandemic associated with COVID-19 (Actividad física en estudiantes deportistas universitarios, previo y en el confinamiento por pandemia asociada al COVID-19)

Retos ◽

10.47197/retos.v0i39.81293 ◽

2020 ◽

pp. 572-575

Author(s):

Edgar Ismael Alarcón Meza ◽

Javier Arturo Hall-López

Keyword(s):

Physical Activity ◽

Student Athletes ◽

Baja California ◽

University Student ◽

Activity Level ◽

Para Determinar ◽

Student’S T ◽

The University ◽

University Athletes ◽

Activity Questionnaire

Abstract. Objective: To compare the levels of physical activity in university athletes before and during the confinement due to pandemic associated with COVID-19. Method: thirty-two university student athletes enrolled in the Sports Faculty of the Autonomous University of Baja California. The average age of the participating subjects was 21.4 ± 3.6 years (17 men and 15 women). To assess the physical activity level, the international physical activity questionnaire (IPAQ) was used; measurements were carried out before and during confinement by COVID-19. Results: To determine equality of variance, the Student's t-test for independent samples was applied, with less physical activity (-33.6 Δ%), MET-minutes/week during confinement by COVID-19 (p=.005). Conclusions: Despite decreasing physical activity, the university athlete performed physical activity at home during confinement by COVID-19, harmonizing the time in the learning processes in their future profession. Resumen. Objetivo: Comparar los niveles de actividad física en deportistas universitarios antes y durante del confinamiento por pandemia asociada al COVID-19. Método: treinta y dos estudiantes deportistas universitarios matriculados en la Facultad de Deportes de la Universidad Autónoma de Baja California. La edad promedio de los sujetos participantes fue 21.4±3.6 años (17 hombres y 15 mujeres). Para evaluar los niveles de actividad física, se utilizó el cuestionario internacional de actividad física (IPAQ), las mediciones se llevaron a cabo previo y en el confinamiento por pandemia asociada al COVID-19. Resultados: Para determinar la igualdad de varianza, la prueba t de Student para muestras independientes que resultó con menor actividad física (-33.6 Δ%), MET-minutos/semana antes que durante el confinamiento por COVID-19 (p=.005). Conclusiones: A pesar de disminuir la actividad física, el deportista universitario realizó actividad física en su hogar durante el confinamiento por COVID-19, armonizando el tiempo en los procesos de aprendizaje en su futura profesión.

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Clinical and psychosocial stress factors are associated with decline in physical activity over time in children with juvenile idiopathic arthritis

Pediatric Rheumatology ◽

10.1186/s12969-021-00584-4 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Liane D. Heale ◽

Kristin M. Houghton ◽

Elham Rezaei ◽

Adam D. G. Baxter-Jones ◽

Susan M. Tupper ◽

...

Keyword(s):

Physical Activity ◽

Juvenile Idiopathic Arthritis ◽

Disease Activity ◽

Psychosocial Stress ◽

Juvenile Arthritis ◽

Stress Factors ◽

Healthy Children ◽

Newly Diagnosed ◽

Z Score ◽

Over Time

Abstract Background Physical activity (PA) patterns in children with juvenile idiopathic arthritis (JIA) over time are not well described. The aim of this study was to describe associations of physical activity (PA) with disease activity, function, pain, and psychosocial stress in the 2 years following diagnosis in an inception cohort of children with juvenile idiopathic arthritis (JIA). Methods In 82 children with newly diagnosed JIA, PA levels, prospectively determined at enrollment, 12 and 24 months using the Physical Activity Questionnaire for Children (PAQ-C) and Adolescents (PAQ-A) raw scores, were evaluated in relation to disease activity as reflected by arthritis activity (Juvenile Arthritis Disease Activity Score (JADAS-71)), function, pain, and psychosocial stresses using a linear mixed model approach. Results in the JIA cohort were compared to normative Pediatric Bone Mineral Accrual Study data derived from healthy children using z-scores. Results At enrollment, PA z-score levels of study participants were lower than those in the normative population (median z-score − 0.356; p = 0.005). At enrollment, PA raw scores were negatively associated with the psychosocial domain of the Juvenile Arthritis Quality of Life Questionnaire (r = − 0.251; p = 0.023). There was a significant decline in PAQ-C/A raw scores from baseline (median and IQR: 2.6, 1.4–3.1) to 24 months (median and IQR: 2.1, 1.4–2.7; p = 0.003). The linear mixed-effect model showed that PAQ-C/A raw scores in children with JIA decreased as age, disease duration, and ESR increased. The PAQ-C/A raw scores of the participants was also negatively influenced by an increase in disease activity as measured by the JADAS-71 (p < 0.001). Conclusion Canadian children with newly diagnosed JIA have lower PA levels than healthy children. The decline in PA levels over time was associated with disease activity and higher disease-specific psychosocial stress.

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Waning antibody responses in COVID-19: what can we learn from the analysis of other coronaviruses?

Infection ◽

10.1007/s15010-021-01664-z ◽

2021 ◽

Author(s):

Ali Hamady ◽

JinJu Lee ◽

Zuzanna A. Loboda

Keyword(s):

Cross Reactivity ◽

Antibody Responses ◽

The Novel ◽

Incidence And Mortality ◽

Antibody Titres ◽

Human Coronaviruses ◽

Public Health Strategies ◽

Antibody Dynamics

Abstract Objectives The coronavirus disease 2019 (COVID-19), caused by the novel betacoronavirus severe acute respiratory syndrome 2 (SARS-CoV-2), was declared a pandemic in March 2020. Due to the continuing surge in incidence and mortality globally, determining whether protective, long-term immunity develops after initial infection or vaccination has become critical. Methods/Results In this narrative review, we evaluate the latest understanding of antibody-mediated immunity to SARS-CoV-2 and to other coronaviruses (SARS-CoV, Middle East respiratory syndrome coronavirus and the four endemic human coronaviruses) in order to predict the consequences of antibody waning on long-term immunity against SARS-CoV-2. We summarise their antibody dynamics, including the potential effects of cross-reactivity and antibody waning on vaccination and other public health strategies. At present, based on our comparison with other coronaviruses we estimate that natural antibody-mediated protection for SARS-CoV-2 is likely to last for 1–2 years and therefore, if vaccine-induced antibodies follow a similar course, booster doses may be required. However, other factors such as memory B- and T-cells and new viral strains will also affect the duration of both natural and vaccine-mediated immunity. Conclusion Overall, antibody titres required for protection are yet to be established and inaccuracies of serological methods may be affecting this. We expect that with standardisation of serological testing and studies with longer follow-up, the implications of antibody waning will become clearer.

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Sequential Seasonal H1N1 Influenza Virus Infections Protect Ferrets against Novel 2009 H1N1 Influenza Virus

Journal of Virology ◽

10.1128/jvi.02257-12 ◽

2012 ◽

Vol 87 (3) ◽

pp. 1400-1410 ◽

Cited By ~ 45

Author(s):

Donald M. Carter ◽

Chalise E. Bloom ◽

Eduardo J. M. Nascimento ◽

Ernesto T. A. Marques ◽

Jodi K. Craigo ◽

...

Keyword(s):

Influenza Virus ◽

H1n1 Influenza ◽

Cross Reactivity ◽

Influenza Viruses ◽

The Novel ◽

Virus Infections ◽

H1n1 Influenza Virus ◽

Virus Shedding ◽

2009 H1n1 ◽

Novel H1n1 Influenza

ABSTRACTIndividuals <60 years of age had the lowest incidence of infection, with ∼25% of these people having preexisting, cross-reactive antibodies to novel 2009 H1N1 influenza. Many people >60 years old also had preexisting antibodies to novel H1N1. These observations are puzzling because the seasonal H1N1 viruses circulating during the last 60 years were not antigenically similar to novel H1N1. We therefore hypothesized that a sequence of exposures to antigenically different seasonal H1N1 viruses can elicit an antibody response that protects against novel 2009 H1N1. Ferrets were preinfected with seasonal H1N1 viruses and assessed for cross-reactive antibodies to novel H1N1. Serum from infected ferrets was assayed for cross-reactivity to both seasonal and novel 2009 H1N1 strains. These results were compared to those of ferrets that were sequentially infected with H1N1 viruses isolated prior to 1957 or more-recently isolated viruses. Following seroconversion, ferrets were challenged with novel H1N1 influenza virus and assessed for viral titers in the nasal wash, morbidity, and mortality. There was no hemagglutination inhibition (HAI) cross-reactivity in ferrets infected with any single seasonal H1N1 influenza viruses, with limited protection to challenge. However, sequential H1N1 influenza infections reduced the incidence of disease and elicited cross-reactive antibodies to novel H1N1 isolates. The amount and duration of virus shedding and the frequency of transmission following novel H1N1 challenge were reduced. Exposure to multiple seasonal H1N1 influenza viruses, and not to any single H1N1 influenza virus, elicits a breadth of antibodies that neutralize novel H1N1 even though the host was never exposed to the novel H1N1 influenza viruses.

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Levels of physical activity in four domains and affective wellbeing before and during the Covid-19 pandemic

Archives of Public Health ◽

10.1186/s13690-021-00651-y ◽

2021 ◽

Vol 79 (1) ◽

Author(s):

Eliane S. Engels ◽

Michael Mutz ◽

Yolanda Demetriou ◽

Anne K. Reimers

Keyword(s):

Mental Health ◽

Physical Activity ◽

Protective Factor ◽

Total Sample ◽

Poor Mental Health ◽

German Population ◽

Time Points ◽

Sport And Exercise ◽

Over Time ◽

Time Point

Abstract Background Latest studies indicated that the general mental health level is low during the pandemic. Probably, this deterioration of the mental health situation is partly due to declines in physical activity. The aim of this study was to investigate differences in and the association between affective wellbeing and levels of different domains of physical activity at three time points before and during the pandemic. Method We used a nationwide online panel with a trend data design encompassing a total sample of N = 3517, representing the German population (> 14 years). Four different activity domains (sport and exercise, light outdoor activity, housework/gardening, active travel) and affective wellbeing (positive and negative affect) were assessed at three time points before and during the Covid-19 pandemic (October 2019, March 2020, October 2020). Results Multivariate analyses of variance (MANOVA) indicate differences regarding affective wellbeing over the three time points with the lowest values at the second time point. Levels of activity in the four domains differed significantly over time with the strongest decrease for sport and exercise from the first to the second time point. Partial correlations indicated that the relationships between sport and exercise and positive affect were most consistent over time. Conclusions Overall, our findings suggest that physical activity plays a particularly important role in the pandemic period as a protective factor against poor mental health. Especially sports and exercise seem to be supportive and should be encouraged, e.g. by providing additional support in finding adequate outdoor, home-based or digital substitutes.

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Customary physical activity and odds of depression: a systematic review and meta-analysis of 111 prospective cohort studies

British Journal of Sports Medicine ◽

10.1136/bjsports-2020-103140 ◽

2021 ◽

pp. bjsports-2020-103140

Author(s):

Rodney K Dishman ◽

Cillian P McDowell ◽

Matthew Payton Herring

Keyword(s):

Physical Activity ◽

Systematic Review ◽

Depressive Symptoms ◽

Cohort Studies ◽

Prospective Cohort ◽

Observational Studies ◽

Meta Analysis ◽

Vigorous Physical Activity ◽

Prospective Cohort Studies ◽

Over Time

ObjectiveTo explore whether physical activity is inversely associated with the onset of depression, we quantified the cumulative association of customary physical activity with incident depression and with an increase in subclinical depressive symptoms over time as reported from prospective observational studies.DesignSystematic review and meta-analysis.Data sourcesMEDLINE, PsycINFO, PsycARTICLES and CINAHL Complete databases, supplemented by Google Scholar.Eligibility criteriaProspective cohort studies in adults, published prior to January 2020, reporting associations between physical activity and depression.Study appraisal and synthesisMultilevel random-effects meta-analysis was performed adjusting for study and cohort or region. Mixed-model meta-regression of putative modifiers.ResultsSearches yielded 111 reports including over 3 million adults sampled from 11 nations in five continents. Odds of incident cases of depression or an increase in subclinical depressive symptoms were reduced after exposure to physical activity (OR, 95% CI) in crude (0.69, 0.63 to 0.75; I2=93.7) and adjusted (0.79, 0.75 to 0.82; I2=87.6) analyses. Results were materially the same for incident depression and subclinical symptoms. Odds were lower after moderate or vigorous physical activity that met public health guidelines than after light physical activity. These odds were also lower when exposure to physical activity increased over time during a study period compared with the odds when physical activity was captured as a single baseline measure of exposure.ConclusionCustomary and increasing levels of moderate-to-vigorous physical activity in observational studies are inversely associated with incident depression and the onset of subclinical depressive symptoms among adults regardless of global region, gender, age or follow-up period.

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Protein Intake and Physical Activity in Newly Diagnosed Patients with Acute Coronary Syndrome: A 5-Year Longitudinal Study

Nutrients ◽

10.3390/nu13020634 ◽

2021 ◽

Vol 13 (2) ◽

pp. 634 ◽

Cited By ~ 1

Author(s):

Andrea Greco ◽

Agostino Brugnera ◽

Roberta Adorni ◽

Marco D’Addario ◽

Francesco Fattirolli ◽

...

Keyword(s):

Physical Activity ◽

Acute Coronary Syndrome ◽

Protein Intake ◽

Processed Meat ◽

High Morbidity ◽

Newly Diagnosed ◽

Healthy Behaviors ◽

Coronary Syndrome ◽

Over Time

Cardiovascular disease is one of the most common causes of hospitalization and is associated with high morbidity and mortality rates. Among the most important modifiable and well-known risk factors are an unhealthy diet and sedentary lifestyle. Nevertheless, adherence to healthy lifestyle regimes is poor. The present study examined longitudinal trajectories (pre-event, 6-, 12-, 24-, 36-, and 60-month follow-ups) of protein intake (fish, legumes, red/processed meat) and physical activity in 275 newly-diagnosed patients with acute coronary syndrome. Hierarchical Generalized Linear Models were performed, controlling for demographic and clinical variables, the season in which each assessment was made, and the presence of anxiety and depressive symptoms. Significant changes in protein intake and physical activity were found from pre-event to the six-month follow-up, suggesting the adoption of healthier behaviors. However, soon after the six-month follow-up, patients experienced significant declines in their healthy behaviors. Both physical activity and red/processed meat intake were modulated by the season in which the assessments took place and by anxiety symptoms over time. The negative long-term trajectory of healthy behaviors suggests that tailored interventions are needed that sustain patients’ capabilities to self-regulate their behaviors over time and consider patient preference in function of season.

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