scholarly journals Early Postoperative Effects of Uncomplicated Phacoemulsification Surgery on Corneal Endothelial Cells and Thickness in Patients with Pseudoexfoliation Syndrome

2021 ◽  
Author(s):  
EREN EKİCİ ◽  
Ali Keles ◽  
Süleyman Korhan Kahraman
Keyword(s):  
Endothelial Cells ◽  
Anterior Chamber ◽  
Coefficient Of Variation ◽  
Corneal Edema ◽  
Pseudoexfoliation Syndrome ◽  
Cell Area ◽  
Corneal Endothelial Cells ◽  
Hexagonal Cell ◽  
Significant Group ◽  
Cell Ratio

Abstract Purpose: To compare early postoperative effects of uncomplicated phacoemulsification surgery on corneal endothelial cells and thickness in patients with pseudoexfoliation syndrome (PEX).Methods: One eye each of 32 patients with PEX and 32 age-matched non-PEX subjects was evaluated preoperatively and on 1st, 7th, and 30th days after uncomplicated phacoemulsification surgery in this retrospective case-control study. Nuclear firmness, corneal edema (CE), anterior chamber reaction (ACR) intensity were graded by a slit-lamp microscope. Endothelial cell density (ECD), coefficient of variation in cell area (CV), hexagonal cell ratio (HEX), and central corneal thickness (CCT) were measured using a noncontact specular microscope.Results: There was no significant group-difference in age, sex, corneal edema (CE), anterior chamber reaction (ACR), coefficient of variation in cell area (CV), and hexagonal cell ratio (HEX). Mean effective phaco time (EPT) was significantly lower intraoperatively (p<0.001) and logarithm of the minimum angle of resolution (logMAR) values of best-corrected visual acuity (BCVA) were significantly higher on both 1st (p<0.001), 7th (p=0.011), and 30th (p=0.025) days postoperatively in the PEX group than in the non-PEX group. Mean ECD was significantly lower in the PEX group than in the non-PEX group on 7th (p=0.013), and 30th (p=0.037) days postoperatively. The mean CCT significantly differed only on 1st (p<0.001) day postoperatively.Conclusion: Eyes with PEX presented lower corneal ECD and decreased BCVA after uncomplicated phacoemulsification surgery. Further, there was no association between CCT and PEX existence preoperatively and in the early postoperative period.

scholarly journals The impact of phacoemulsification on corneal endothelial cells in patients with pseudoexfoliation syndrome

2019 ◽  
Vol 12 (1) ◽  
pp. 50-55
Author(s):  
V. V. Potemkin ◽  
T. S. Varganova ◽  
E. V. Ageeva
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Coefficient Of Variation ◽  
Negative Impact ◽  
Corneal Endothelial Cell ◽  
Pseudoexfoliation Syndrome ◽  
Corneal Endothelial Cells ◽  
Before And After ◽  
Corneal Endothelial Cell Density ◽  
The Impact

The effect of pseudoexfoliation syndrome (PEХ) on endothelial cells has been studied long enough. Yet the effect of phacoemulsification (PHACO) on endothelium in patients with PEХ is less explored.Purpose. To assess the impact of PHACO on corneal endothelial cell density (ECD) and on the coefficient of variation (CV) in patients with PEX.Material and methods. 30 patients (30 eyes) with PEX syndrome and 34 patients (34 eyes) with no such syndrome were examined before and after phacoemulsification.Results. In patients with PEX, the ECD after PHACO was significantly lower and CV was significantly higher (р < 0.05).Conclusion. PEX has a negative impact on endothelial cells, which leads to a pronounced cells loss after PHACO.

scholarly journals Scanning electron microscopy of the corneal endothelium of ostrich

2009 ◽  
Vol 39 (3) ◽  
pp. 926-929 ◽  
Author(s):  
João Antonio Tadeu Pigatto ◽  
Angela Aguiar Franzen ◽  
Fabiana Quartiero Pereira ◽  
Ana Carolina da Veiga Rodarte de Almeida ◽  
José Luis Laus ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endothelial Cells ◽  
Cell Density ◽  
Coefficient Of Variation ◽  
Corneal Endothelium ◽  
Cell Area ◽  
Corneal Endothelial Cells ◽  
Average Cell ◽  
Scanning Electron

The aim of this study was to examine the endothelial surface morphology and perform a morphometric analysis of the corneal endothelial cells of ostrich (Struthio camelus) using scanning electron microscopy. Polygonality, mean cell area, cell density and coefficient of variation of mean cell area were analyzed. The normal corneal endothelium consisted of polygonal cells of uniform size and shape with few interdigitations of the cell borders. Microvilli appeared as protusions on the cellular surface. The average cell area was 269±18µm² and the endothelial cell density was 3717±240cells mm-2. The coefficient of variation of the cell area was 0.06, and the percentage of hexagonal cells was 75%. The parameters evaluated did not differ significantly between the right and the left eye from the same ostrich. The results of this study showed that the ostrich corneal endothelial cells appear quite similar to those of the other vertebrates.

scholarly journals MitoROS due to loss of Slc4a11 in corneal endothelial cells induces ER stress, lysosomal dysfunction and impairs autophagy

2020 ◽  
Author(s):  
Rajalekshmy Shyam ◽  
Diego G. Ogando ◽  
Moonjung Choi ◽  
Joseph A. Bonanno
Keyword(s):  
Endothelial Cells ◽  
Er Stress ◽  
Corneal Edema ◽  
Corneal Endothelial Cells ◽  
Autophagy Flux ◽  
Unfolded Protein ◽  
Stress Markers ◽  
Lysosomal Dysfunction ◽  
Mitochondrial Ros ◽  
Elevated Expression

AbstractRecent studies from Slc4a11 KO mice have identified mitochondrial dysfunction as a major contributor toward oxidative stress and cell death in Congenital Hereditary Endothelial Dystrophy. Here we asked if this stress activated autophagy in the Slc4a11 KO cell line and in KO mouse endothelial tissue. Early indicators of autophagy, phospho-mTOR and LC3-II indicated activation, however P62 was elevated suggesting an impairment of autophagy flux. The activity and the number of lysosomes, the organelle responsible for the final degradation of autophagy substrates, were found to be reduced in the KO. In addition, the expression of the master regulator of lysosomal function and biogenesis, TFEB, was significantly reduced in the KO corneal endothelia. Also, we observed increased Unfolded Protein Response, as well as elevated expression of ER stress markers, BIP and CHOP. To test if lysosomal and ER stress stems from elevated mitochondrial ROS, we treated Slc4a11 KO corneal endothelial cells with the mitochondrial ROS quencher, MitoQ. MitoQ restored lysosomal enzymes as well as TFEB, reduced ER stress, and increased autophagy flux. MitoQ injections of Slc4a11 KO mice decreased corneal edema, the major phenotype associated with CHED. We conclude that mitochondrial ROS causes ER stress and lysosomal dysfunction with impairment of autophagy in Slc4a11 KO corneal endothelium. Our study is the first to identify the presence as well as cause of lysosomal dysfunction and ER stress in an animal model of CHED, and to characterize inter-organelle relationship in a corneal cell type.

scholarly journals Utilização do flap de Gundersen para tratamento de disfunção endotelial em equino – relato de caso

2021 ◽  
Vol 4 (5) ◽  
pp. 32-38
Author(s):  
Cleydianne Rodrigues de Almeida ◽  
◽  
Cleyber José da Trindade de Fátima ◽  
Rômulo Vitelli Rocha Peixoto ◽  
Anderson Farias ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Dysfunction ◽  
Corneal Ulcer ◽  
Corneal Edema ◽  
Corneal Endothelial Cells ◽  
Eye Care ◽  
Visual Axis ◽  
Lens Dislocation ◽  
Uveitis Anterior ◽  
Ulcerative Keratitis

Endothelial dysfunction in horses is associated with dystrophy or degeneration of corneal endothelial cells, clinically presented as a diffuse corneal edema unresponsive to conventional treatments. The main causes of such injury are trauma, ulcerative keratitis, recurrent uveitis, anterior lens dislocation and glaucoma. This paper aims to report a case of endothelial dysfunction in a mare, diagnosed with endothelial dysfunction after uveitis, glaucoma and indolent corneal ulcer. For correction, a superficial lamellar keratectomy followed by permanent conjunctival graft, described as a Gundersen flap, was performed. After intensive eye care, he returned to his athletic functions, maintained corneal and visual axis transparency and ocular reflexes.

scholarly journals NAD+ precursors protect corneal endothelial cells from UVB-induced apoptosis

2020 ◽  
Vol 318 (4) ◽  
pp. C796-C805 ◽  
Author(s):  
Can Zhao ◽  
Wenjing Li ◽  
Haoyun Duan ◽  
Zongyi Li ◽  
Yanni Jia ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Apoptosis ◽  
Corneal Endothelium ◽  
Corneal Edema ◽  
Ultraviolet B ◽  
Subconjunctival Injection ◽  
Corneal Endothelial Cells ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Mesenchymal Transition ◽  
Induced Apoptosis

Excessive exposure of the eye to ultraviolet B light (UVB) leads to corneal edema and opacification because of the apoptosis of the corneal endothelium. Our previous study found that nicotinamide (NIC), the precursor of nicotinamide adenine dinucleotide (NAD), could inhibit the endothelial-mesenchymal transition and accelerate healing the wound to the corneal endothelium in the rabbit. Here we hypothesize that NIC may possess the capacity to protect the cornea from UVB-induced endothelial apoptosis. Therefore, a mouse model and a cultured cell model were used to examine the effect of NAD+ precursors, including NIC, nicotinamide mononucleotide (NMN), and NAD, on the UVB-induced apoptosis of corneal endothelial cells (CECs). The results showed that UVB irradiation caused apparent corneal edema and cell apoptosis in mice, accompanied by reduced levels of NAD+ and its key biosynthesis enzyme, nicotinamide phosphoribosyltransferase (NAMPT), in the corneal endothelium. However, the subconjunctival injection of NIC, NMN, or NAD+ effectively prevented UVB-induced tissue damage and endothelial cell apoptosis in the mouse cornea. Moreover, pretreatment using NIC, NMN, and NAD+ increased the survival rate and inhibited the apoptosis of cultured human CECs irradiated by UVB. Mechanistically, pretreatment using nicotinamide (NIC) recovered the AKT activation level and decreased the BAX/BCL-2 ratio. In addition, the capacity of NIC to protect CECs was fully reversed in the presence of the AKT inhibitor LY294002. Therefore, we conclude that NAD+ precursors can effectively prevent the apoptosis of the corneal endothelium through reactivating AKT signaling; this represents a potential therapeutic approach for preventing UVB-induced corneal damage.

scholarly journals Comparison of the Changes in Corneal Endothelial Cells after Pars Plana and Anterior Chamber Ahmed Valve Implant

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ji Won Seo ◽  
Jong Yeon Lee ◽  
Dong Heun Nam ◽  
Dae Yeong Lee
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Anterior Chamber ◽  
Cell Damage ◽  
Corneal Endothelial Cells ◽  
Refractory Glaucoma ◽  
Endothelial Cell Damage ◽  
Significant Difference ◽  
Pars Plana

Purpose. To compare the changes in corneal endothelial cells after pars plana Ahmed glaucoma valve (AGV) implantation with those after the anterior chamber AGV implantation for refractory glaucoma.Methods. The medical records of 18 eyes with pars plana implantation of AGV (ppAGV) were reviewed retrospectively and were compared with 18 eyes with the anterior chamber AGV (acAGV) implant. The preoperative and postoperative endothelial cells, intraocular pressure (IOP), and postoperative complications during the follow-up in both groups were compared.Results. The average follow-up was 18 months. The postoperative endothelial cells in the ppAGV and acAGV groups were 2044 ± 303 and 1904 ± 324, respectively(P=0.25). The average percentage decrease in the endothelial cells in the ppAGV and acAGV groups at 18 months was 12.5% and 18.4%, respectively, and showed significant difference between the 2 groups(P=0.01). No difference in IOP control and the number of postoperative glaucoma medications was observed between the 2 groups.Conclusions. Endothelial cell damage in the ppAGV group for refractory glaucoma appeared to be lower than that in the acAGV group. Therefore, pars plana implantation of AGV may be preferred as it may have lower level of endothelial cell damage while maintaining similar level of IOP control.

scholarly journals Persimmon Leaves (Diospyros kaki) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na+-K+-ATPase Activity

2022 ◽  
Vol 15 (1) ◽  
pp. 72
Author(s):  
Ramsha Afzal ◽  
Hyung Bin Hwang
Keyword(s):  
Endothelial Cells ◽  
Atpase Activity ◽  
Basolateral Membrane ◽  
Corneal Edema ◽  
Ethanol Extract ◽  
Diospyros Kaki ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells ◽  
Corneal Decompensation

The Na+/K+-ATPase, present in the basolateral membrane of human corneal endothelial cells (HCECs), is known to play an important role for corneal transparency. Na+/K+-ATPase dysfunction is one of the major causes of corneal decompensation. The ethanol extract of Diospyros kaki (EEDK) has been reported to increase corneal cell viability. Thus, we treated HCECs with EEDK and studied its effects on HCECs survival and Na+/K+-ATPase against cytotoxic drugs like staurosporine (ST) and ouabain (OU). Firstly, survival assays, (MTT assay and live dead-imaging) showed that decreased HCECs viability by ST and OU was significantly recovered by EEDK co-treatment. Secondly, Na+/K+-ATPase activity assays revealed that EEDK enhanced Na+/K+-ATPase enzymatic activity (* p < 0.01) with/without ST and OU. Finally, Na+/K+-ATPase expression analysis (Western Blot and confocal microscopy) demonstrated that EEDK treatment with/without ST and OU facilitates Na+/K+-ATPase expression in HCECs. Taken together, our findings led us to the conclusion that EEDK might aid HCECs survival in vitro by increasing the activity and expression of Na+/K+-ATPase enzyme. Since Na+/K+-ATPase activity is important to maintain cellular function of HCECs, we suggest that EEDK can be a potential effective agent against corneal edema and related corneal disorders.

scholarly journals Long-Term Destiny of Corneal Endothelial Cells in Anterior Chamber Intraocular Lens-Implanted Eyes

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Yating Tang ◽  
Jie Xu ◽  
Jiahui Chen ◽  
Yi Lu
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Anterior Chamber ◽  
Cell Density ◽  
Intraocular Lens ◽  
Corneal Endothelial Cells ◽  
Corneal Decompensation ◽  
The Mean

Purpose. To investigate the long-term changes of corneal endothelial cells (EC) in anterior chamber intraocular lens- (AC-IOL-) implanted eyes. Methods. Retrospective study. We included 37 eyes (25 patients) that received AC-IOL implantation previously in the Eye and ENT Hospital of Fudan University between 1995 and 2016. Follow-up outcomes included the best-corrected visual acuity (BCVA), endothelial cell density, hexagonality, coefficient of variance, and central corneal thickness. Results. In total, 23 eyes (62.16%) with phakic and 14 eyes (37.84%) with aphakic AC-IOLs were included. Among these, 3 eyes (8.11%) were angle-supported AC-IOLs and 34 eyes (91.89%) were Artisan iris-fixated AC-IOLs. The mean age of patients was 41.40 ± 17.17 years, and the mean follow-up time was 12.12 ± 4.71 years in our study. At the follow-up time, corneal decompensation existed in 3 angle-supported AC-IOL eyes with a rate of 100% and 15 iris-fixated AC-IOL eyes with a rate of 44.12%. AC-IOL displacement occurred in 14 (41.18%) iris-fixated AC-IOL eyes. In the 19 iris-fixated AC-IOL eyes without corneal decompensation, significant changes also took place in corneal endothelial cells. The endothelial cell density decreased from 2843.26 ± 300.76 to 2015.58 ± 567.99 cells/mm2 (29.1% loss, P < 0.001 ) and hexagonality decreased from 51.21 ± 7.83 to 42.53 ± 9.17 (%) (16.9% loss, P < 0.001 ). The Kaplan–Meier survival curve also demonstrated the accumulated expectation rates of corneal endothelial cell decomposition for AC-IOLs with a median survival time of 12 years. Conclusion. We reported a significant chronic loss and long-term decompensation destiny of corneal endothelial cells in AC-IOL eyes. Semiannual or annual follow-up and evaluation of endothelial cells should be conducted in AC-IOL-implanted patients.

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