Screening of Antifungal Substances from Bovistella Radicata (Mont.) Pat and Their Antifungal Effect
Abstract Background To analyse the antifungal active compounds in B. radicata alcohol extract, the alcohol extract was purified by column chromatography (macroporous resinD-101) and the active compounds was named as SPAF-1(the spore powder active fraction). Results Alcohol extracts and SPAF-1 were submitted to GC-MS analysis, there were two characteristic peaks(peak1 and peak2) in Gas chromatogram. By comparing in NIST, the compound were 2-propyl-1-pentanol corresponding to peak1 and decanal, n-decanol and 2E-decanol corresponding to peak2. The main constituents were decanal(24.3%) n-decanol(27.9%), 2E-decenol (21.2%) and 2-Propyl-1-pentanol (13.6%). Their MIC values were 62.5 µg/ml, 31.2 µg/ml, 31.2 µg/ml and 250 µg/ml against T. rubrum respectively. Furthermore, transmission electron microscope (TEM) analysis showed altered surface morphology in the majority of T. rubrum cells after treatment with SPAF-1. Conclusions In this paper, we successfully separate SPAF-1 from alcohol extract of B. radicata. The antifungal effect of SPAF-1 is similar to positive control, the main component were decanal, n-decanol, 2E-decenol and 2-Propyl-1-pentanol, the anti-tinea pedis effect of them was obvious.