scholarly journals Acute Bone Damage Through Liver-bone Axis Induced by Thioacetamide in Rats

2020 ◽  
Author(s):  
Yang Li ◽  
Jianghua Li ◽  
Linyan Cheng ◽  
Yetao Yao ◽  
Hao Shen ◽  
...  
Keyword(s):  
Liver Damage ◽  
Rat Model ◽  
Bone Structure ◽  
Organ Damage ◽  
Test System ◽  
Organic Chemical ◽  
Micro Ct ◽  
Serum Samples ◽  
Bone Damage ◽  
Ct Analysis

Abstract Background: Thioacetamide（TAA）is used in various fields, such as synthetic drugs, organic chemical synthesis, and materials chemistry. In the medical field, TAA is mainly used to establish animal liver injury models and other organ damage models to explore their mechanisms for helping patients with liver disease, however, TAA caused bone damage is barely understood. Therefore, the aim of our study consisted in building a rat model reflecting the TAA-treated caused acute bone damage. Results: Serum samples collected from 5-times TAA-treated rats and were used in biochemical test, we found the level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA), total bile acid (TBA), alkaline phosphatase (ALP), carbamide (UREA) and creatinine (CREA) exhibited sharply rise, while the level of serum content of total protein (TP), lactate dehydrogenase (LDH), calcium (Ca) and phosphorus (P) were severely reduced. At the same time, we obtained some data about cortical bone and trabecular bone by Micro-CT analysis, it revealed significantly decreased bone surface, tissue surface, bone volume, tissue volume in TAA-treated rats, moreover, we used a static biomechanical test system to test the femoral force range of the hind limbs of SD rats, we found bone can resist less pressure and it is easy to take fracture. Conclusions: Summarizing, our rat model presents possible mediators of liver damage, liver damage and changes in bone structure and mineralization are already visible by Micro-CT analysis after five-times of TAA treatment. The fast response and easy building possibly make it an ideal model to investigate bone metabolism in liver damage after they were affected by TAA.

scholarly journals The comparative Micro-CT analysis on trabecular bone density between hydroxyapatite gypsum puger scaffold application and bovine hydroxyapatite scaffold application

2021 ◽  
Vol 54 (1) ◽  
pp. 11
Author(s):  
Amiyatun Naini
Keyword(s):  
Bone Density ◽  
Trabecular Bone ◽  
Wistar Rats ◽  
Micro Ct ◽  
Trabecular Bone Density ◽  
Bone Damage ◽  
Significant Difference ◽  
Ct Analysis ◽  
Bovine Hydroxyapatite ◽  
Test Result

Background: Generally, after tooth extraction, trauma is caused by bone damage, which leads to a decreased bone density. Bone damage repair should be conducted using a bone graft containing hydroxyapatite (HA). HA can be synthesised from gypsum puger powder, which is abundant and easy to obtain. Hydroxyapatite gypsum puger (HAGP) was successful with 100% hydroxyapatite purity level. Purpose: To compare the ratio of trabecular bone density in Wistar rats between HAGP scaffold application and bovine hydroxyapatite (BHA) scaffold application. Methods: This study is a laboratory experiment using 6 treatment groups, namely K (-) polyethylene glycol (PEG) 7, K (-) PEG 28, HAGP + PEG 7, HAGP + PEG 28, BHA + PEG 7, and BHA + PEG 28. HAGP scaffold freeze-drying. The rats were anaesthetised intramuscularly, and their left mandibular incisor was removed. The scaffold was applied to the mouse socket, followed by tissue decapitation after 7 and 28 days. The examination was carried out with micro-computed tomography (Micro-CT). Next, statistical analysis using a one-way analysis of variance (ANOVA) test was conducted (p <0.05). Results: The ANOVA test result showed a difference in bone density between the treatment and control groups on days 7 and 28. The Least Significant Difference (LSD) test result revealed that there was no significant difference between K (-) PEG 28 and HAGP + PEG 7 (p=0.133). Nevertheless, there were significant differences between the other groups. Conclusion: Based on the Micro-CT analysis, the trabecular bone density in Wistar rats following HAGP scaffold application is higher than that of BHA scaffold application.

The effects of Labisia pumila on postmenopausal osteoporotic rat model: Dose and time-dependent micro-CT analysis

2014 ◽  
Vol 22 (4) ◽  
pp. 503-518 ◽  
Author(s):  
Nadia Mohd Effendy ◽  
Mohd Fadhli Khamis ◽  
Ima Nirwana Soelaiman ◽  
Ahmad Nazrun Shuid
Keyword(s):  
Rat Model ◽  
Time Dependent ◽  
Micro Ct ◽  
Ct Analysis ◽  
Labisia Pumila

scholarly journals Strontium Incorporated Coralline Hydroxyapatite for Engineering Bone

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Waiching Liu ◽  
Ting Wang ◽  
Yuhui Shen ◽  
Haobo Pan ◽  
Songlin Peng ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Rat Model ◽  
Bone Growth ◽  
Micro Ct ◽  
Coralline Hydroxyapatite ◽  
Ct Analysis ◽  
Surface Morphologies ◽  
Volume Retention

Goniopora was hydrothermally converted to coralline hydroxyapatite (CHA) and incorporated with Sr (Sr-CHA). The pore size of Goniopora was in the range of 40–300 μm with a porosity of about 68%. Surface morphologies of the coral were modified to flake-like hydroxyapatite structures on CHA and the addition of Sr detected on Sr-CHA as confirmed by SEM and EDX. As the first report of incorporating Sr into coral, about 6%–14% Sr was detected on Sr-CHA. The compressive strengths of CHA and Sr-CHA were not compromised due to the hydrothermal treatments. Sr-CHA was studied in vitro using MC3T3-E1 cells and in vivo with an ovariectomized rat model. The proliferation of MC3T3-E1 cells was significantly promoted by Sr-CHA as compared to CHA. Moreover, higher scaffold volume retention (+40%) was reported on the micro-CT analysis of the Sr-CHA scaffold. The results suggest that the incorporation of Sr in CHA can further enhance the osteoconductivity and osteoinductivity of corals. Strontium has been suggested to stimulate bone growth and inhibit bone resorption. In this study, we have successfully incorporated Sr into CHA with the natural porous structure remained and explored the idea of Sr-CHA as a potential scaffolding material for bone regeneration.

The evaluation of the multiple myeloma pathological osseous tissue of Microarchitecture

2020 ◽  
Vol 16 ◽  
Author(s):  
Lianxiang Chen ◽  
Xing Wang ◽  
Shaojie Zhang ◽  
Yidan Wang ◽  
Baoke Su ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Structure ◽  
Imaging Finding ◽  
Structural Parameters ◽  
Bone Destruction ◽  
Micro Ct ◽  
Osteolytic Lesions ◽  
Microstructural Damage ◽  
Specificity And Sensitivity ◽  
Bone Damage

Background: The typical imaging finding of MM is bone destruction in the form of diffuse patchy osteolytic lesions or punctate destruction. However, it is difficult to accurately determine the fine structure of bone affected by MM with these techniques due to low specificity and sensitivity. Introduction: This study aimed to investigate the microscopic anatomical morphology and analyze the microstructure changes of trabeculae affected by multiple myeloma (MM) based on micro-CT. Methods: MM-affected and normal trabecular bone samples were imaged by micro-CT to obtain bone structure parameters to assess statistical differences between them and evaluate the degree of microstructural damage of MMaffected trabeculae. Results: Micro CT images clearly showed the microstructure of MM-affected trabeculae. The degree of trabecular osteoporosis varied with the severity of MM. There were significant differences in the structural parameters between MMaffected and normal trabeculae (P < 0.05). Conclusions: Micro-CT clearly reveals the microstructure of MM-affected trabeculae. The obtained bone structure data will help to determine the degree of bone damage caused by MM and assess the efficacy.

scholarly journals Comparison of Vascular Morphometry in Jawbones and Long Bones: Micro-CT Study in a Rat Model Treated with Zoledronic Acid

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Jing Yi Wang ◽  
Ru Qing Yu ◽  
Lei Huo ◽  
Nian Jing Rao ◽  
Weijia William Lu ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Rat Model ◽  
Tooth Extraction ◽  
Statistical Difference ◽  
Female Rats ◽  
Long Bones ◽  
Micro Ct ◽  
Sprague Dawley ◽  
Vascular Perfusion ◽  
Ct Analysis

The study was aimed at investigating the effect of zoledronic acid on vascular morphometry in jawbones and long bones on a rat model. Twenty-four skeletal mature Sprague-Dawley female rats were administered oncologic dose of zoledronic acid (ZA) or normal saline for 4 weeks and then subjected to tooth extraction on the mandible and maxilla and a bone defect creation on the femur. After the surgical procedures, ZA or saline treatment was continued until sacrifice at week 2, week 4, and week 8 postoperatively. Vascular perfusion with MICROFIL was performed on all the animals. Micro-CT analysis demonstrated a tendency of decreased vessel density and vessel number in ZA-treated groups but no statistical difference. In conclusion, the neovessel formation is suppressed but not significantly by ZA treatment, indicating that angiogenesis inhibition may contribute to the development of MRONJ but does not play a key role.

Periosteal distraction osteogenesis versus immediate periosteal elevation in a rat model: Histological and micro-CT analysis

2017 ◽  
Vol 45 (5) ◽  
pp. 620-627 ◽  
Author(s):  
Ken Nakahara ◽  
Maiko Haga-Tsujimura ◽  
Kosaku Sawada ◽  
Matthias Mottini ◽  
Benoit Schaller ◽  
...  
Keyword(s):  
Distraction Osteogenesis ◽  
Rat Model ◽  
Micro Ct ◽  
Ct Analysis

scholarly journals Micro CT analysis of the subarticular bone structure in the area of the talar trochlea

2013 ◽  
Vol 35 (4) ◽  
pp. 283-293 ◽  
Author(s):  
Andrej Maria Nowakowski ◽  
Hans Deyhle ◽  
Silvan Zander ◽  
André Leumann ◽  
Magdalena Müller-Gerbl
Keyword(s):  
Bone Structure ◽  
Micro Ct ◽  
Ct Analysis

scholarly journals Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

2020 ◽  
Vol 10 (12) ◽  
pp. 4328 ◽  
Author(s):  
Ilaria Campioni ◽  
Raffaella Pecci ◽  
Rossella Bedini
Keyword(s):  
Bone Structure ◽  
Ex Vivo ◽  
Maxillofacial Surgery ◽  
Three Dimensional ◽  
3D Models ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Scientific Publications ◽  
Ct Analysis

Micro-computed tomography (micro-CT) is a consolidated imaging technology allowing non-destructive three-dimensional (3D) qualitative and quantitative analysis by the observation of microstructures with high resolution. This paper aims at delivering a structured overview of literature about studies performed using micro-CT in dentistry and maxillofacial surgery (MFS) by analyzing the entire set of articles to portray the state of the art of the last ten years of scientific publications on the topic. It draws the scenario focusing on biomaterials, in vitro and in/ex vivo applications, bone structure analysis, and tissue engineering. It confirms the relevance of the micro-CT analysis for traditional research applications and mainly in dentistry with respect to MFS. Possible developments are discussed in relation to the use of the micro-CT combined with other, traditional, and not, techniques and technologies, as the elaboration of 3D models based on micro-CT images and emerging numerical methods. Micro-CT results contribute effectively with whose ones obtained from other techniques in an integrated multimethod approach and for multidisciplinary studies, opening new possibilities and potential opportunities for the next decades of developments.

scholarly journals Modelling the skeletal muscle injury recovery using in vivo contrast-enhanced micro-CT: a proof-of-concept study in a rat model

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Bruno Paun ◽  
Daniel García Leon ◽  
Alex Claveria Cabello ◽  
Roso Mares Pages ◽  
Elena de la Calle Vargas ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rat Model ◽  
Muscle Injury ◽  
Micro Ct ◽  
Skeletal Muscle Injury ◽  
Monitoring Time ◽  
Contrast Enhanced Ct ◽  
Contrast Enhanced ◽  
Injury Recovery

Abstract Background Skeletal muscle injury characterisation during healing supports trauma prognosis. Given the potential interest of computed tomography (CT) in muscle diseases and lack of in vivo CT methodology to image skeletal muscle wound healing, we tracked skeletal muscle injury recovery using in vivo micro-CT in a rat model to obtain a predictive model. Methods Skeletal muscle injury was performed in 23 rats. Twenty animals were sorted into five groups to image lesion recovery at 2, 4, 7, 10, or 14 days after injury using contrast-enhanced micro-CT. Injury volumes were quantified using a semiautomatic image processing, and these values were used to build a prediction model. The remaining 3 rats were imaged at all monitoring time points as validation. Predictions were compared with Bland-Altman analysis. Results Optimal contrast agent dose was found to be 20 mL/kg injected at 400 μL/min. Injury volumes showed a decreasing tendency from day 0 (32.3 ± 12.0mm3, mean ± standard deviation) to day 2, 4, 7, 10, and 14 after injury (19.6 ± 12.6, 11.0 ± 6.7, 8.2 ± 7.7, 5.7 ± 3.9, and 4.5 ± 4.8 mm3, respectively). Groups with single monitoring time point did not yield significant differences with the validation group lesions. Further exponential model training with single follow-up data (R2 = 0.968) to predict injury recovery in the validation cohort gave a predictions root mean squared error of 6.8 ± 5.4 mm3. Further prediction analysis yielded a bias of 2.327. Conclusion Contrast-enhanced CT allowed in vivo tracking of skeletal muscle injury recovery in rat.

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