The effects of Labisia pumila on postmenopausal osteoporotic rat model: Dose and time-dependent micro-CT analysis

Goniopora was hydrothermally converted to coralline hydroxyapatite (CHA) and incorporated with Sr (Sr-CHA). The pore size of Goniopora was in the range of 40–300 μm with a porosity of about 68%. Surface morphologies of the coral were modified to flake-like hydroxyapatite structures on CHA and the addition of Sr detected on Sr-CHA as confirmed by SEM and EDX. As the first report of incorporating Sr into coral, about 6%–14% Sr was detected on Sr-CHA. The compressive strengths of CHA and Sr-CHA were not compromised due to the hydrothermal treatments. Sr-CHA was studied in vitro using MC3T3-E1 cells and in vivo with an ovariectomized rat model. The proliferation of MC3T3-E1 cells was significantly promoted by Sr-CHA as compared to CHA. Moreover, higher scaffold volume retention (+40%) was reported on the micro-CT analysis of the Sr-CHA scaffold. The results suggest that the incorporation of Sr in CHA can further enhance the osteoconductivity and osteoinductivity of corals. Strontium has been suggested to stimulate bone growth and inhibit bone resorption. In this study, we have successfully incorporated Sr into CHA with the natural porous structure remained and explored the idea of Sr-CHA as a potential scaffolding material for bone regeneration.

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Comparison of Vascular Morphometry in Jawbones and Long Bones: Micro-CT Study in a Rat Model Treated with Zoledronic Acid

BioMed Research International ◽

10.1155/2021/6651318 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Jing Yi Wang ◽

Ru Qing Yu ◽

Lei Huo ◽

Nian Jing Rao ◽

Weijia William Lu ◽

...

Keyword(s):

Zoledronic Acid ◽

Rat Model ◽

Tooth Extraction ◽

Statistical Difference ◽

Female Rats ◽

Long Bones ◽

Micro Ct ◽

Sprague Dawley ◽

Vascular Perfusion ◽

Ct Analysis

The study was aimed at investigating the effect of zoledronic acid on vascular morphometry in jawbones and long bones on a rat model. Twenty-four skeletal mature Sprague-Dawley female rats were administered oncologic dose of zoledronic acid (ZA) or normal saline for 4 weeks and then subjected to tooth extraction on the mandible and maxilla and a bone defect creation on the femur. After the surgical procedures, ZA or saline treatment was continued until sacrifice at week 2, week 4, and week 8 postoperatively. Vascular perfusion with MICROFIL was performed on all the animals. Micro-CT analysis demonstrated a tendency of decreased vessel density and vessel number in ZA-treated groups but no statistical difference. In conclusion, the neovessel formation is suppressed but not significantly by ZA treatment, indicating that angiogenesis inhibition may contribute to the development of MRONJ but does not play a key role.

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Acute Bone Damage Through Liver-bone Axis Induced by Thioacetamide in Rats

10.21203/rs.3.rs-36674/v1 ◽

2020 ◽

Author(s):

Yang Li ◽

Jianghua Li ◽

Linyan Cheng ◽

Yetao Yao ◽

Hao Shen ◽

...

Keyword(s):

Liver Damage ◽

Rat Model ◽

Bone Structure ◽

Organ Damage ◽

Test System ◽

Organic Chemical ◽

Micro Ct ◽

Serum Samples ◽

Bone Damage ◽

Ct Analysis

Abstract Background: Thioacetamide（TAA）is used in various fields, such as synthetic drugs, organic chemical synthesis, and materials chemistry. In the medical field, TAA is mainly used to establish animal liver injury models and other organ damage models to explore their mechanisms for helping patients with liver disease, however, TAA caused bone damage is barely understood. Therefore, the aim of our study consisted in building a rat model reflecting the TAA-treated caused acute bone damage. Results: Serum samples collected from 5-times TAA-treated rats and were used in biochemical test, we found the level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA), total bile acid (TBA), alkaline phosphatase (ALP), carbamide (UREA) and creatinine (CREA) exhibited sharply rise, while the level of serum content of total protein (TP), lactate dehydrogenase (LDH), calcium (Ca) and phosphorus (P) were severely reduced. At the same time, we obtained some data about cortical bone and trabecular bone by Micro-CT analysis, it revealed significantly decreased bone surface, tissue surface, bone volume, tissue volume in TAA-treated rats, moreover, we used a static biomechanical test system to test the femoral force range of the hind limbs of SD rats, we found bone can resist less pressure and it is easy to take fracture. Conclusions: Summarizing, our rat model presents possible mediators of liver damage, liver damage and changes in bone structure and mineralization are already visible by Micro-CT analysis after five-times of TAA treatment. The fast response and easy building possibly make it an ideal model to investigate bone metabolism in liver damage after they were affected by TAA.

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Periosteal distraction osteogenesis versus immediate periosteal elevation in a rat model: Histological and micro-CT analysis

Journal of Cranio-Maxillofacial Surgery ◽

10.1016/j.jcms.2017.01.030 ◽

2017 ◽

Vol 45 (5) ◽

pp. 620-627 ◽

Cited By ~ 1

Author(s):

Ken Nakahara ◽

Maiko Haga-Tsujimura ◽

Kosaku Sawada ◽

Matthias Mottini ◽

Benoit Schaller ◽

...

Keyword(s):

Distraction Osteogenesis ◽

Rat Model ◽

Micro Ct ◽

Ct Analysis

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Time-dependent changes in inflammatory markers in a rat model of colitis by leisure sport activity

Zeitschrift für Gastroenterologie ◽

10.1055/s-0033-1347518 ◽

2013 ◽

Vol 51 (05) ◽

Author(s):

Z Szalai ◽

K Kupai ◽

A Magyariné Berkó ◽

A Pósa ◽

R Szabó ◽

...

Keyword(s):

Rat Model ◽

Inflammatory Markers ◽

Sport Activity ◽

Time Dependent

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Modelling the skeletal muscle injury recovery using in vivo contrast-enhanced micro-CT: a proof-of-concept study in a rat model

European Radiology Experimental ◽

10.1186/s41747-020-00163-4 ◽

2020 ◽

Vol 4 (1) ◽

Cited By ~ 1

Author(s):

Bruno Paun ◽

Daniel García Leon ◽

Alex Claveria Cabello ◽

Roso Mares Pages ◽

Elena de la Calle Vargas ◽

...

Keyword(s):

Skeletal Muscle ◽

Rat Model ◽

Muscle Injury ◽

Micro Ct ◽

Skeletal Muscle Injury ◽

Monitoring Time ◽

Contrast Enhanced Ct ◽

Contrast Enhanced ◽

Injury Recovery

Abstract Background Skeletal muscle injury characterisation during healing supports trauma prognosis. Given the potential interest of computed tomography (CT) in muscle diseases and lack of in vivo CT methodology to image skeletal muscle wound healing, we tracked skeletal muscle injury recovery using in vivo micro-CT in a rat model to obtain a predictive model. Methods Skeletal muscle injury was performed in 23 rats. Twenty animals were sorted into five groups to image lesion recovery at 2, 4, 7, 10, or 14 days after injury using contrast-enhanced micro-CT. Injury volumes were quantified using a semiautomatic image processing, and these values were used to build a prediction model. The remaining 3 rats were imaged at all monitoring time points as validation. Predictions were compared with Bland-Altman analysis. Results Optimal contrast agent dose was found to be 20 mL/kg injected at 400 μL/min. Injury volumes showed a decreasing tendency from day 0 (32.3 ± 12.0mm3, mean ± standard deviation) to day 2, 4, 7, 10, and 14 after injury (19.6 ± 12.6, 11.0 ± 6.7, 8.2 ± 7.7, 5.7 ± 3.9, and 4.5 ± 4.8 mm3, respectively). Groups with single monitoring time point did not yield significant differences with the validation group lesions. Further exponential model training with single follow-up data (R2 = 0.968) to predict injury recovery in the validation cohort gave a predictions root mean squared error of 6.8 ± 5.4 mm3. Further prediction analysis yielded a bias of 2.327. Conclusion Contrast-enhanced CT allowed in vivo tracking of skeletal muscle injury recovery in rat.

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Micro‐CT analysis of the Lisfranc complex reveals higher bone mineral density in dorsal compared to plantar regions

Journal of Orthopaedic Research® ◽

10.1002/jor.25164 ◽

2021 ◽

Author(s):

Melissa R. Requist ◽

Yantarat Sripanich ◽

Tim Rolvien ◽

Amy L. Lenz ◽

Alexej Barg

Keyword(s):

Bone Mineral Density ◽

Bone Mineral ◽

Micro Ct ◽

Mineral Density ◽

Ct Analysis

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A Micro-Computed Tomography Comparison of the Porosity in Additively Fabricated CuCr1 Alloy Parts Using Virgin and Surface-Modified Powders

Materials ◽

10.3390/ma14081995 ◽

2021 ◽

Vol 14 (8) ◽

pp. 1995

Author(s):

Mirko Sinico ◽

Suraj Dinkar Jadhav ◽

Ann Witvrouw ◽

Kim Vanmeensel ◽

Wim Dewulf

Keyword(s):

High Energy ◽

High Energy Density ◽

Micro Ct ◽

Micro Computed Tomography ◽

Powder Bed ◽

Metallurgical Defects ◽

Ct Analysis ◽

Ct Data ◽

Surface Modified ◽

Fusion Pores

Recently, the use of novel CuCr1 surface-modified powder for reliable laser powder-bed fusion (LPBF) manufacturing has been proposed, enabling a broader LPBF processing window and longer powder storage life. Nevertheless, virgin CuCr1 powder is also LPBF processable, on the condition that a high-energy density is employed. In this work, we compare two dense specimens produced from virgin and surface-modified CuCr1 powder. Furthermore, a third sample fabricated from surface-modified powder is characterized to understand an abnormal porosity content initially detected through Archimedes testing. Utilizing high-resolution micro-CT scans, the nature of the defects present in the different samples is revealed. Pores are analyzed in terms of size, morphology and spatial distribution. The micro-CT data reveal that the virgin CuCr1 dense specimen displays keyhole pores plus pit cavities spanning multiple layer thicknesses. On the other hand, the sample fabricated with the surface-modified CuCr1 powder mainly contains small and spherical equi-distributed metallurgical defects. Finally, the CT analysis of the third specimen reveals the presence of a W contamination, favoring lack-of-fusion pores between subsequent LPBF layers. The LPBF melting mode (keyhole or conductive), the properties of the material, and the potential presence of contaminants are connected to the different porosity types and discussed.

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