scholarly journals Overexpression of Nitric Oxide and Inducible Nitric Oxide Synthase in Mucous Membrane of the Maxillary Sinus Fungus Ball

2020 ◽  
Author(s):  
Hang-jin Li ◽  
Yan Guo ◽  
Bo Yu ◽  
Ling-yan Zhang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Maxillary Sinus ◽  
Inflammatory Cells ◽  
Control Group ◽  
Fungus Ball ◽  
Spearman Correlation ◽  
Oxide Synthase ◽  
And Control ◽  
Sinus Mucosa ◽  
Better Than

Abstract Objective: We aimed to explore the expression and interplay of NO and iNOS to elucidate their roles in human maxillary sinus mucosa with maxillary sinus fungus ball (MSFB) and chronic rhinosinusitis (CRS).Methods: Fifty-one patients with MSFB, CRS and simple maxillary sinus cyst were included in this study. The NO content of the mucosa of each group was detected by Nitric acid reductase method. The expression of iNOS was tested by immunohistochemistry and Western blott. Spearman correlation analysis was used to analyze the correlation between the expression of iNOS and the occurrence of MSFB and CRS.Results: The results showed that NO and iNOS were highly expressed in the maxillary sinus mucosa of MSFB group, which were significantly different from CRS and control group (P<0.01). The expressions of NO and iNOS in the samples of CRS group were higher than that of the control group (P<0.05). Moreover, iNOS was widely presented in the cytoplasm of sinus mucosa epithelium and inflammatory cells in the maxillary sinus mucosa of MSFB patients. Furthermore, Spearman correlation coefficient indicated that the expression of iNOS was positively correlated with the incidence of MSFB (r=0.6395, P<0.05).Conclusion: NO and iNOS are overexpressed in the maxillary sinus mucosa of MSFB and CRS patients, and the increase in the MSFB patients was better than the CRS. The expressions of NO, iNOS were also correlated with the incidence of MSFB. We thus provide a new indicator to assist the diagnosis of MSFB and its distinction from CRS.

Effect of chronic lithium administration on endothelium-dependent relaxation of rat mesenteric bed: role of nitric oxide

2007 ◽  
Vol 85 (10) ◽  
pp. 1038-1046 ◽  
Author(s):  
Banafsheh Afsharimani ◽  
Leila Moezi ◽  
Hamed Sadeghipour ◽  
Bahareh Rahimzadeh-Rofouyi ◽  
Maliheh Nobakht ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nadph Diaphorase ◽  
Control Group ◽  
No Donor ◽  
Vascular Bed ◽  
Mesenteric Vascular Bed ◽  
Vascular Beds ◽  
Oxide Synthase ◽  
And Control

The mechanism of action of lithium, an effective treatment for bipolar disease, is still unknown. In this study, the mesenteric vascular beds of control rats and rats that were chronically treated with lithium were prepared by the McGregor method, and the mesenteric vascular bed vasorelaxation responses were examined. NADPH-diaphorase histochemistry was used to determine the activity of NOS (nitric oxide synthase) in mesenteric vascular beds. We demonstrated that ACh-induced vasorelaxation increased in the mesenteric vascular bed of rats treated with lithium. Acute Nο-nitro-l-arginine methyl ester (l-NAME) administration in the medium blocked ACh-induced vasorelaxation in the control group more effectively than in lithium-treated rats, while the vasorelaxant response to sodium nitroprusside, a NO donor, was not different between lithium-treated and control groups. Acute aminoguanidine administration blocked ACh-induced vasorelaxation of lithium-treated rats, but had no effect in the control rats. Furthermore, NOS activity, determined by NADPH-diaphorase staining, was significantly greater in the mesenteric vascular beds from chronic lithium-treated rats than in those from control rats. These data suggest that the enhanced ACh-induced endothelium-derived vasorelaxation in rat mesenteric bed from chronic lithium-treated rats might be associated with increased NOS activity, likely via iNOS. Simultaneous acute l-NAME and indomethacin administration suggests the possible upregulation of EDHF (endothelium-derived hyperpolarizing factor) in lithium-treated rats.

The effect of cadmium toxicity on renal nitric oxide synthase isoenzymes

2011 ◽  
Vol 28 (7) ◽  
pp. 624-628 ◽  
Author(s):  
Sedat Soyupek ◽  
Taylan Oksay ◽  
Recep Sütçü ◽  
Abdullah Armağan ◽  
Osman Gökalp ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Western Blot ◽  
Histopathological Examination ◽  
Cadmium Toxicity ◽  
Renal Physiology ◽  
Control Group ◽  
Cd Toxicity ◽  
Oxide Synthase ◽  
And Control

The aim of this study was to assess the cadmium (Cd) toxicity on renal nitric oxide synthase (NOS) isoenzymes. The study was carried out on 18 inbred male (Cd group: 10 and control group: 8) Wistar rats. Cd group received drinking water containing 15 mg/L Cd for 30 days; and at the end of the 30 days, plasma Cd was analysed. One kidney was snap frozen to assess the endothelial NOS (eNOS), inducible NOS (iNOS) and neuronal NOS (nNOS) expressions by Western blot analyses, and the other kidney was preserved for histopathological examination. Plasma Cd levels were significantly elevated in the Cd group. The Western blot analyses found higher levels of eNOS, iNOS and nNOS in the Cd group but only eNOS and nNOS levels were statistically significant. There was no difference in pathological assessment of the renal tissues. Cd toxicity increases NOS isoenzyme levels and may affect renal physiology.

scholarly journals Does the maxillary sinus have a triggering role in nasal nitric oxide synthesis?

2012 ◽  
Vol 50 (4) ◽  
pp. 402-407
Author(s):  
O. Güçlü ◽  
A. Uludağ ◽  
A. Akçalı ◽  
K. Tekin ◽  
H. Erdoğan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Streptococcus Pneumoniae ◽  
Nitric Oxide Synthase ◽  
Maxillary Sinus ◽  
Nasal Mucosa ◽  
Control Group ◽  
Nitric Oxide Synthesis ◽  
Nasal Nitric Oxide ◽  
Exhaled Air ◽  
Oxide Synthase

Objectives: We investigated whether the maxillary sinus plays a stimulatory role in nasal nitric oxide (NO) synthesis. Research on sinusitis and nasal polyps has found low NO levels in exhaled air and linked this to obstruction of the ostium. However, the major source of NO in exhaled air is thought to be the nasal mucosa. In this study, Streptococcus pneumoniae was applied to the maxillary sinus to investigate changes in NO synthesis of the nasal mucosa. Methods: An experimental study was performed with New Zealand white rabbits. Three groups, pneumococcus, control and sham, were created. The maxillary sinus of the pneumococcal group was exposed to Streptococcus pneumoniae suspension. Before and after the exposure, bilateral biopsy specimens were taken from the inferior turbinate. Specimens were examined by RT-PCR for expressions of endothelial nitric oxide synthase (e-NOS) and inducible nitric oxide synthase (i-NOS). Physiological saline solution was administered to the maxillary sinus in the control group and biopsies were obtained. The sham group underwent only biopsy. Results: A significant increase in i-NOS expression of tissue samples from the pneumococcal group on the same and opposite sides were detected. There was no increase in e-NOS expression in this group. The control and sham groups had no significant change in i-NOS or e-NOS expression. Conclusion: In the acute period after the maxillary sinus is exposed to a pathogen, i-NOS expression increases in the nasal mucosa, but endothelial NOS expression is not affected. Consequently, a combined response in the maxillary sinus and the nasal mucosa for nitric oxide synthesis is shown in the present study.

CO2 laser enhances the chilling tolerance of wheat seedlings by stimulating NO synthesis

2016 ◽  
Vol 96 (5) ◽  
pp. 796-807
Author(s):  
Yi-ping Chen ◽  
Qiang Liu ◽  
Dong Chen
Keyword(s):  
Nitric Oxide ◽  
Laser Irradiation ◽  
Sodium Tungstate ◽  
Chilling Stress ◽  
Chilling Tolerance ◽  
The Other ◽  
Control Group ◽  
Wheat Seedlings ◽  
Oxide Synthase ◽  
Protein Treatment

To investigate the mechanism by which laser irradiation enhances the chilling tolerance of wheat seedlings, seeds were exposed to different treatments, and biochemical parameters were measured. Compared with the control group, chilling stress (CS) led to an increase in the concentrations of malondialdehyde (MDA) and H2O2, and decreases in the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), catalase (CAT), peroxidase (POD), and nitric oxide synthase (NOS), and the concentrations of nitric oxide (NO) and protein. Treatment with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), sodium tungstate (ST), and NG-nitro-L-arginine methyl ester (L-NAME) followed by CS resulted in further increases in the concentrations of MDA and H2O2 and further decreases in the other parameters. However, treatment with PTIO, ST, and L-NAME followed by laser irradiation had the opposite effects on these parameters. When the seeds were treated with PTIO, ST, and L-NAME followed by laser and CS, the concentrations of MDA and H2O2 were significantly lower and the other parameters were higher than in the PTIO, ST, and L-NAME plus CS groups. These results suggest that CO2 laser irradiation enhances the chilling tolerance of wheat seedlings by stimulating endogenous NO synthesis.

Selective iNOS Inhibition Attenuates Acetylcholine- and Bradykinin-induced Vasoconstriction in Lipopolysaccharide-exposed Rat Lungs 

1999 ◽  
Vol 91 (6) ◽  
pp. 1724-1724 ◽  
Author(s):  
Lars G. Fischer ◽  
Damian J. Horstman ◽  
Klaus Hahnenkamp ◽  
Nancy E. Kechner ◽  
George F. Rich
Keyword(s):  
Nitric Oxide ◽  
Exhaled Nitric Oxide ◽  
Control Group ◽  
Biochemical Engineering ◽  
Inos Inhibitor ◽  
Nos Inhibitor ◽  
Inos Inhibitors ◽  
Oxide Synthase ◽  
Inos Inhibition ◽  
Dose Dependent

Background Nonselective nitric oxide synthase (NOS) inhibition has detrimental effects in sepsis because of inhibition of the physiologically important endothelial NOS (eNOS). The authors hypothesized that selective inducible NOS (iNOS) inhibition would maintain eNOS vasodilation but prevent acetylcholine- and bradykinin-mediated vasoconstriction caused by lipopolysaccharide-induced endothelial dysfunction. Methods Rats were administered intraperitoneal lipopolysaccharide (15 mg/kg) with and without the selective iNOS inhibitors L-N6-(1-iminoethyl)-lysine (L-NIL, 3 mg/kg), dexamethasone (1 mg/kg), or the nonselective NOS inhibitor Nomega-nitro-L-arginine methylester (L-NAME, 5 mg/kg). Six hours later, the lungs were isolated and pulmonary vasoreactivity was assessed with hypoxic vasoconstrictions (3% O2), acetylcholine (1 microg), Biochemical Engineering, and bradykinin (3 microg). In additional lipopolysaccharide experiments, L-NIL (10 microM) or 4-Diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP, 100 microM), a selective muscarinic M3 antagonist, was added into the perfusate. Results Exhaled nitric oxide was higher in the lipopolysaccharide group (37.7+/-17.8 ppb) compared with the control group (0.4+/-0.7 ppb). L-NIL and dexamethasone decreased exhaled nitric oxide in lipopolysaccharide rats by 83 and 79%, respectively, whereas L-NAME had no effect. In control lungs, L-NAME significantly decreased acetylcholine- and bradykinin-induced vasodilation by 75% and increased hypoxic vasoconstrictions, whereas L-NIL and dexamethasone had no effect. In lipopolysaccharide lungs, acetylcholine and bradykinin both transiently increased the pulmonary artery pressure by 8.4+/-2.0 mmHg and 35.3+/-11.7 mmHg, respectively, immediately after vasodilation. L-NIL and dexamethasone both attenuated this vasoconstriction by 70%, whereas L-NAME did not. The acetylcholine vasoconstriction was dose-dependent (0.01-1.0 microg), unaffected by L-NIL added to the perfusate, and abolished by 4-DAMP. Conclusions In isolated perfused lungs, acetylcholine and bradykinin caused vasoconstriction in lipopolysaccharide-treated rats. This vasoconstriction was attenuated by administration of the iNOS inhibitor L-NIL but not with L-NAME. Furthermore, L-NIL administered with lipopolysaccharide preserved endothelium nitric oxide-dependent vasodilation, whereas L-NAME did not.

Selected Contribution: Differential role of nitric oxide synthase isoforms in fever of different etiologies: studies using Nosgene-deficient mice

2003 ◽  
Vol 94 (6) ◽  
pp. 2534-2544 ◽  
Author(s):  
Wieslaw Kozak ◽  
Anna Kozak
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Corn Oil ◽  
Normal Male ◽  
Wild Type ◽  
Oxide Synthase ◽  
And Control ◽  
Nos Isoforms ◽  
Deficient Mice

Male C57BL/6J mice deficient in nitric oxide synthase (NOS) genes (knockout) and control (wild-type) mice were implanted intra-abdominally with battery-operated miniature biotelemeters (model VMFH MiniMitter, Sunriver, OR) to monitor changes in body temperature. Intravenous injection of lipopolysaccharide (LPS; 50 μg/kg) was used to trigger fever in response to systemic inflammation in mice. To induce a febrile response to localized inflammation, the mice were injected subcutaneously with pure turpentine oil (30 μl/animal) into the left hindlimb. Oral administration (gavage) of N G-monomethyl-l-arginine (l-NMMA) for 3 days (80 mg · kg−1 · day−1in corn oil) before injection of pyrogens was used to inhibit all three NOSs ( N G-monomethyl-d-arginine acetate salt and corn oil were used as control). In normal male C57BL/6J mice, l-NMMA inhibited the LPS-induced fever by ∼60%, whereas it augmented fever by ∼65% in mice injected with turpentine. Challenging the respective NOS knockout mice with LPS and with l-NMMA revealed that inducible NOS and neuronal NOS isoforms are responsible for the induction of fever to LPS, whereas endothelial NOS (eNOS) is not involved. In contrast, none of the NOS isoforms appeared to trigger fever to turpentine. Inhibition of eNOS, however, exacerbates fever in mice treated with l-NMMA and turpentine, indicating that eNOS participates in the antipyretic mechanism. These data support the hypothesis that nitric oxide is a regulator of fever. Its action differs, however, depending on the pyrogen used and the NOS isoform.

scholarly journals PENGGUNAAN STRATEGI PEMBELAJARAN AKTIF EVERYONE IS A TEACHER HERE (ETH) TERHADAP KEMAMPUAN PEMAHAMAN KONSEP MATEMATIKA SISWA KELAS VII

2018 ◽  
Vol 4 (1) ◽  
pp. 95-100
Author(s):  
Sri Yunita Ningsih ◽  
Gustimalasari Gustimalasari
Keyword(s):  
Active Learning ◽  
Learning Strategy ◽  
Control Group ◽  
Control Group Design ◽  
Concept Understanding ◽  
Group Design ◽  
Quasi Experimental ◽  
Active Learning Strategy ◽  
And Control ◽  
Better Than

Abstract. This research has been made to know skill of student’s concept by using active learning strategy everyone is teacher here (ETH). Beside that this study aims to measure student’s concept understanding with statistical test between Experimental Class (Active Learning Strategy Everyone Is Teacher Here) and control class (Conventional Learning ). The population was seventh grade of SMPN 3 Lirik consist 94 students in three classes. Sample was took randomly, experiment class ( VII.2 ) and control class ( VII.I ) This research was experiment, the form of this research was Quasi Experimental Design with randomized subject posttest only control group design. based on statistic data processing has been retrieved - t hitung -3,159 smaller than - t table was -2,000 and based on t test has been retrieved -thitung < -t table so Ho rejected and Ha received. So that the writer conclude that skill of math student’s concept understanding by using active learning Strategy Everyone Is Teacher Here (ETH) is better than conventional concept understanding.Keywords: Everyone Is A Teacher Here, Concept Understanding

scholarly journals THE IMPACT OF TRANSLANGUAGING APPROACH ON TEACHING ARABIC READING IN A MULTILINGUAL CLASSROOM

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Saidna Zulfiqar Bin-Tahir ◽  
Ummu Saidah ◽  
Nuril Mufidah ◽  
Riki Bugis
Keyword(s):  
Reading Comprehension ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Control Group ◽  
Education Department ◽  
Written Test ◽  
Multilingual Classroom ◽  
And Control ◽  
The Impact ◽  
Better Than

 The study aimed at measuring the effect of translanguaging approach in teaching Arabic reading comprehension to the second semester students of Indonesian Education Department, Universitas Iqra Buru. 64 students involved as the participants through simple random sampling technique. 32 students participated in each of the experimental and control group. The researchers used reading test of written test to obtain information about the students’ achievemnt in reading on the use of translanguaging approach and it was distributed to the respondents after the last treatment given. To find out the mean score, standard deviation and the t-test value between the pretest and the posttest the researchers used the SPSS 17 program. The researchers found that the use of translanguaging approach in learning Arabic reading comprehension improves the students’ ability of Arabic reading comprehension is better than the conventional method.

scholarly journals The Influence of Teaching Vocabulary by Using Picture

2016 ◽  
Vol 1 (2) ◽  
pp. 159
Author(s):  
Hanik Nuzulimah
Keyword(s):  
Control Group ◽  
First Year ◽  
First Year Students ◽  
Significant Difference ◽  
Post Test ◽  
The Subject ◽  
And Control ◽  
Data Analysis Result ◽  
Experimental Group ◽  
Better Than

This paper attempts to explore whether there is significant difference of achievement in vocabulary between students taught by using pictures and those are not. The subject of this study is the first year students of SLTP Muhammadiyah Simo Susukan, Semarang where the sample contains 50 students that is divided into experimental group (with treatment) and control group (without treatment). The data is gathered from participants’ score obtained from pre test and post test. Using t test as technique of data analysis, result shows that students taught by using pictures perform better than those are not. It means that there is significant difference between the two groups. Keywords: Vocabulary Teaching; Picture

scholarly journals The Effect of Using Word Family Game on The Vocabulary Mastery of The Fifth Grade Students at SD Negeri 1 Astina in Academic Years of 2017/2018

2017 ◽  
Vol 1 (3) ◽  
pp. 122
Author(s):  
Ni Komang Amelia Kartadi
Keyword(s):  
Fifth Grade ◽  
Control Group ◽  
Control Group Design ◽  
Fifth Grade Students ◽  
Conventional Teaching ◽  
Post Test ◽  
The Mean ◽  
And Control ◽  
Experimental Group ◽  
Better Than

This study aimed at investigating of the effect of using word family game committed by the fifth grade student in SD Negeri 1 Astina in mastering the English vocabulary. This study was True-Experimental with post-test only control group design. The population was 59 students of the fifth grade and the sample was selected by using cluster random sampling. The sample of this research study was 22 students in 5A as an Experimental Group while 22 students in 5B as a Control Group. The experimental group was taught by using Word Family Game and control group was taught without using Word Family Game. The data was analyzed descriptively and inferentially through SPSS 16.0 Program. Descriptively, the students in experimental group were achieved better than the students in control group. It was proven by the result of the mean score of the experimental group was 90.45, while the mean score of control group was 83.18. The result of the t-test also showed that the score of the (tobs) was 3.393 which the score of (tcv) was 1.682 which based on the degree of freedom was 42. It showed that the (tobs) > (tcv), where: 3.393 > 1.682. It could be concluded that the word family game had a significant effect on the students’ vocabulary mastery rather than using conventional teaching. 

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