scholarly journals Expression Level of Annexin A1 Contributes to the Evaluation of the Disease Progression and Treatment Effect of Lung Adenocarcinoma: A New Perspective from Retrospective Analysis

2021 ◽  
Author(s):  
Biaoxue Rong ◽  
Hongling Yan ◽  
Ge Wu ◽  
Kai Li ◽  
Min Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Disease Progression ◽  
Treatment Effect ◽  
Expression Level ◽  
Annexin A1 ◽  
Normal Tissues ◽  
Node Metastasis ◽  
Cancer Tissues ◽  
The Relationship

Abstract Background: Increased Annexin A1 has been showed to be related to malignant biological characteristics of tumors; the aim of this study was to evaluate the relationship between the expression level of Annexin A1 and the disease progression and treatment effect of lung adenocarcinoma (LAC). Methods: The expression level of Annexin A1 in LAC tissues and cells was detected by the methods of immunohistochemistry, Real time-PCR and western blotting. The relationship between the expression of Annexin A1 and the disease progression and treatment effect of LAC was evaluated by descriptive statistics, T test and Chi-square test. Results: The protein expression of Annexin A1 was higher in lung cancer tissues and cells than that in normal tissues and 16 human bronchial epithelial (16HBE) cells (p<0.05). The level of Annexin A1 mRNA was higher in lung cancer tissues than that in normal tissues (p<0.05). The increase of Annexin A1 protein and mRNA was associated with the lymph node metastasis, advanced clinical stage (p<0.05). However, surgical resection and chemotherapy for LAC down-regulated the serum concentration of Annexin A1 in patients (p<0.05).Conclusions: Increased Annexin A1 protein and mRNA in LAC tissues correlate with the poor differentiation, lymph node metastasis and advanced stage of LAC. Surgical resection and chemotherapy for LAC down-regulate the serum concentration of Annexin A1 in patients. The results indicate that expression level of Annexin A1 contributes to the evaluation of the disease progression and treatment effect of LAC.

Relationship between NOB1 expression and prognosis of resected non-small cell lung cancer

2015 ◽  
Vol 30 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Kun Liu ◽  
Hong-Lin Chen ◽  
Ming-Ming Gu ◽  
Qing-Sheng You
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Progression Free Survival ◽  
Small Cell ◽  
Small Cell Lung ◽  
Free Survival ◽  
Node Metastasis ◽  
Resected Nsclc ◽  
The Relationship

Background The aim of this study was to investigate the relationship between Nin one binding (NOB1) protein expression and prognosis for resected non-small cell lung cancer (NSCLC). Methods A prospective cohort of 70 consecutive patients with resected NSCLC was studied in 2009. Immunohistochemistry was used in the detection of NOB1 protein expression. Prognosis outcomes included overall survival (OS) and progression-free survival (PFS). The log-rank test and Cox hazard model were used to estimate the relationship between NOB1 expression and prognosis. Results In the 70 NSCLC tissue specimens, 14 (20%) stained -, 24 (34%) stained +, 21 (30%) stained ++ and 11 (16%) stained +++. The NOB1 high expression rate was 16%. NOB1 expression was significantly different between TMN stage (p=0.024) and lymph node metastasis (p=0.001), as well as histopathological grades (p=0.037). Median OS was 43 months (95% confidence interval [95% CI], 35-51 months), and median PFS was 37 months (95% CI, 25-49 months). OS and PFS were related to TMN stage and lymph node metastasis, as well as NOB1 expression (p<0.05). After adjustment for TMN stage and lymph node metastasis, the hazard ratio (HR) for high NOB1 expression was 1.7 (95% CI, 1.1-3.0, p=0.027) for OS, and 1.8 (95% CI, 1.3-3.7, p=0.031) for PFS. Conclusions Our results suggest that enhanced expression of NOB1 is related to poor overall survival and progression-free survival in patients with resected NSCLC.

scholarly journals Relationship between Hypercoagulable State and Circulating Tumor Cells in Peripheral Blood, Pathological Characteristics, and Prognosis of Lung Cancer Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Yanhua Jiao ◽  
Liling Guo ◽  
Linqian Wu ◽  
Tongshan Hu
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cancer Patients ◽  
Hypercoagulable State ◽  
Test Results ◽  
Lung Cancer Patients ◽  
Node Metastasis ◽  
Pathological Characteristics ◽  
The Relationship

Objective. To analyze the relationship between hypercoagulable state and circulating tumor cells (CTCs) in peripheral blood, pathological characteristics, and prognosis of lung cancer patients. Method. A total of 148 patients with primary lung cancer diagnosed and treated in our hospital from January 2017 to January 2019 were selected as the research objects. According to the CTC test results, the patients were divided into CTC-positive group and CTC-negative group. Also, the coagulation index of patients was tested. According to the blood coagulation index test results, patients were divided into hypercoagulable group and non-hypercoagulable group. The relationship between hypercoagulable state and pathological characteristics of lung cancer patients was analyzed by single factor analysis and multiple logistic regression model. Kaplan–Meier survival curve was applied to analyze the relationship between hypercoagulable state and the prognosis of lung cancer patients. Results. The platelets (PLTs), fibrinogen (FIB), D-dimer (D-D), and prothrombin time (PT) in CTC-positive group were significantly higher than those in CTC-negative group. There was no significant relationship between the patient’s gender, smoking history, pathological type, and the hypercoagulable state of the patients. The proportion of patients aged 60 years or older, with TMN stage III or IV and lymph node metastasis, in the hypercoagulable group was significantly higher than that in the non-hypercoagulable group. Logistic regression analysis showed that there was an independent relationship between the patient’s age, lymph node metastasis, and hypercoagulable state. As of January 2020, among the 148 patients with lung cancer follow-up, 5 patients were lost and 52 died. The median survival time of patients in the hypercoagulable group was 82 weeks, which was significantly lower than the 104 weeks in the nonhypercoagulable group. Conclusion. There is a certain relationship between hypercoagulable state and CTC positive in lung cancer patients. There is an independent relationship between the patient’s age, lymph node metastasis, and the hypercoagulable state. The median survival time of patients in the hypercoagulable group was significantly lower than that in the non-hypercoagulable group.

scholarly journals Loss of Atypical Chemokine Receptors Expression Promotes Lymph Node Metastasis of Colorectal Cancer

2021 ◽  
Author(s):  
Haitao Duan ◽  
Yao Huang ◽  
Caihua Zhang ◽  
Juanjuan Cui ◽  
Lianying Guo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Chemokine Receptors ◽  
Statistical Significance ◽  
Immunohistochemical Analysis ◽  
Pcr Analysis ◽  
Normal Tissues ◽  
Node Metastasis ◽  
Cancer Tissues

Abstract Background: Atypical chemokine receptors (ACKRs), including DARC, D6 and CCX-CKR, hold very critical roles in cancer invasion and metastasis. The objective of the present study was to determine preliminarily the predictive value of ACKRs in colorectal cancer (CRC). Methods: From 2012 to 2019 in the First Affiliated Hospital of Dalian Medical University, 44 CRC patients were followed up. We performed immunohistochemical analysis, Western blot and RT-PCR analysis on CRC and adjacent normal tissues for investigating the expression of ACKRs and their relationships to clinicopathologic features and survival rate. Results: The expression of three ACKRs, CCX-CKR, DARC and D6, in normal colon tissues is higher than that in colorectal cancer tissues. Meanwhile, the expression of corresponding ligands CCL21, CCL2 and CCL22 shows the opposite. Low expression of ACKRs in colon cancer tissues was closely related to the potential of lymph node metastasis (P= 0.001). When compared with triple-negative ACKR expression, co-expression of the ACKRs predicted better outcomes of colorectal cancer patients with statistical significance (P=0.011). Conclusions: The loss of ACKRs may play important roles in lymph node metastasis of CRC. ACKR expression may be considered as prognostic markers in CRC patients.

scholarly journals Expression of the Yes-Associated Protein, β-Catenin, and Smoothened Protein and Their Clinical Significance in Invasive Breast Cancer

2021 ◽  
Author(s):  
Pengju Liu ◽  
Jianfeng Zeng ◽  
Gaohua Yang
Keyword(s):  
Breast Cancer ◽  
Invasive Breast Cancer ◽  
Growth Factor Receptor ◽  
Normal Breast ◽  
Expression Level ◽  
Normal Tissues ◽  
Epidermal Growth ◽  
Cancer Tissues ◽  
The Relationship ◽  
Mcf 7

Abstract Background: The mechanism of the Yes-associated protein in the occurrence and development of breast cancer is still unclear. Here, we aimed to explore the relationship among the Yes-associated protein, β-catenin, and Smoothened signaling pathways to provide a theoretical basis for the clinical diagnosis and Treatment program of invasive breast cancer. Methods: Immunohistochemistry was used to detect the expression of Yes-associated protein, Smoothened protein, and β-catenin in breast cancer, adjacent, and normal tissues. The relationship between the expression of these three proteins and clinicopathological features of breast cancer patients was analyzed, and western blotting was used to detect differences in Yes-associated protein expression in MCF-7 and MDA-MB-231 cell lines. Results: The expression level of Yes-associated protein in breast cancer tissues was prominently Lower than that in normal breast tissues; the expression level of β-catenin in breast cancer tissues was prominently higher than that in normal tissues; and the expression level of Smoothened protein in cancer tissue was Prominently higher than that in normal breast tissue. The expression of Yes-associated protein in breast cancer tissues was correlated with the expression of the human epidermal growth factor receptor 2, progesterone receptor, and estrogen receptor; the expression of β-catenin and Smoothened protein in breast cancer tissues were all correlated with the human epidermal growth factor receptor expression. The expression level of Yes-associated protein in the MCF-7 cells was significantly higher than that in the MDA-MB-231 cells. There was a negative correlation between the Yes-associated protein and Smoothened protein expression in the breast cancer tissues.Conclusions: The Yes-associated protein, β-catenin, and Smoothened protein can be used as new targets in the treatment of invasive breast cancer or as reference indicators for prognostic judgment. The differences in expression of the three proteins in cancer adjacent, cancer, and normal tissues suggest that they may be of great significance in the process of breast-conserving surgery.

scholarly journals The Cell Surface Estrogen Receptor, G Protein- Coupled Receptor 30 (GPR30), is Markedly down Regulated during Breast Tumorigenesis

2008 ◽  
Vol 1 ◽  
pp. BCBCR.S557 ◽  
Author(s):  
Indira Poola ◽  
Jessy Abraham ◽  
Aiyi Liu ◽  
Josephine J. Marshalleck ◽  
Robert L. Dewitty
Keyword(s):  
Estrogen Receptor ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cell Surface ◽  
Mrna Levels ◽  
Breast Tumorigenesis ◽  
Normal Tissues ◽  
Node Metastasis ◽  
Tumor Tissues ◽  
Cancer Tissues

Background GPR30 is a cell surface estrogen receptor that has been shown to mediate a number of non-genomic rapid effects of estrogen and appear to balance the signaling of estrogen and growth factors. In addition, progestins appear to use GPR30 for their actions. Therefore, GPR30 could play a critical role in hormonal regulation of breast epithelial cell integrity. Deregulation of the events mediated by GPR30 could contribute to tumorigenesis. Methods To understand the role of GPR30 in the deregulation of estrogen signaling processes during breast carcinogenesis, we have undertaken this study to investigate its expression at mRNA levels in tumor tissues and their matched normal tissues. We compared its expression at mRNA levels by RT quantitative real-time PCR relative to GAPDH in ERα”—positive (n = 54) and ERα”—negative (n = 45) breast cancer tissues to their matched normal tissues. Results We report here, for the first time, that GPR30 mRNA levels were significantly down-regulated in cancer tissues in comparison with their matched normal tissues (p < 0.0001 by two sided paired t-test). The GPR30 expression levels were significantly lower in tumor tissues from patients (n = 29) who had lymph node metastasis in comparison with tumors from patients (n = 53) who were negative for lymph node metastasis (two sample t-test, p < 0.02), but no association was found with ERα, PR and other tumor characteristics. Conclusions Down-regulation of GPR30 could contribute to breast tumorigenesis and lymph node metastasis.

Increased high-mobility group A2 correlates with lymph node metastasis and prognosis of non-small cell lung cancer

2018 ◽  
Vol 21 (3) ◽  
pp. 547-555 ◽  
Author(s):  
Xiangjun Guo ◽  
Jiaxin Shi ◽  
Yan Wen ◽  
Mengmeng Li ◽  
Qin Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
High Mobility ◽  
Small Cell ◽  
High Mobility Group ◽  
Small Cell Lung ◽  
Node Metastasis

scholarly journals miR-21 Is a Promising Novel Biomarker for Lymph Node Metastasis in Patients with Gastric Cancer

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Yuejuan Xu ◽  
Jue Sun ◽  
Jianhua Xu ◽  
Qi Li ◽  
Yuewu Guo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Malignant Disease ◽  
Close Association ◽  
Survival Rates ◽  
Expression Level ◽  
Human Gastric Cancer ◽  
Node Metastasis ◽  
Kaplan Meier

Background. Gastric cancer (GC) is an important malignant disease around the world. Abnormalities of microRNAs (miRNAs) have been implicated in carcinogenesis of various cancers. In the present study, we examined miR-21 expression in human gastric cancer with lymph node metastasis and attempted to uncover its relationship with clinicopathologic data, especially with lymph node metastasis.Materials and Methods. The expression levels of miR-21 in the tumor specimens of GC patients were quantified by RT-PCR. The correlation between miR-21 level and multiple clinicopathological factors was then examined by Mann-Whitney test, Kaplan-Meier survival analysis, and operating characteristic (ROC) analysis.Results. The expression level of miR-21 was higher in GC patients with lymph node metastasis than in those without lymph node metastasis (P<0.05). Expression level of miR-21 was significantly correlated with histologic type, T stage, lymph node metastasis and pTNM stage. The overall survival rates in GC patients with low upregulated miR-21 expression were significantly higher than those with high upregulated miR-21 (P<0.05).Conclusion. A close association is implicated between the elevated miR-21and lymph node metastasis, which could potentially be exploited as a practical biomarker for lymph node metastasis in patients with GC.

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