scholarly journals Lesion Activity Assessment of Early Caries Using Dye-enhanced Quantitative Light- Induced Fluorescence

2021 ◽  
Author(s):  
Seok-Woo Park ◽  
Si-Mook Kang ◽  
Hyung-Suk Lee ◽  
Sang-Kyeom Kim ◽  
Eun-Song Lee ◽  
...  
Keyword(s):  
Porous Structure ◽  
Fluorescence Intensity ◽  
Active Group ◽  
Sodium Fluorescein ◽  
Dye Penetration ◽  
Activity Assessment ◽  
Carious Lesions ◽  
Significant Difference ◽  
Early Caries ◽  
Caries Lesions

Abstract We aimed to determine whether dye-ehanced quantitative light-induced fluorescence (DEQLF), wherein the porous structure of carious lesions is filled in with a fluorescent dye, can be used to quantitatively and intuitively distinguish between active and inactive carious lesions. To simulate active and inactive caries, lesions were artificially formed on 126 bovine specimens. They were demineralized with 1% carbopol solution for 3 (A3), 5 (A5), and 10 days (A10). Half the specimens in each group were remineralized with 2% NaF (I3, I5, and I10). The specimens were dehydrated for 10 s and dyed with 100-µM sodium fluorescein for 10 s. Images were captured with a QLF-digital 2+ Biluminator. Fluorescence intensity of the lesions (ΔG) between the groups and absolute changes in ΔG (|ΔΔG|) between dehydration and dye penetration were compared using the independent t-test. ΔG in A3, A5, and A10 were higher than those in I3, I5, and I10 (P<0.001). In |ΔΔG| comparisons, dye penetration was 3.1-3.7 times higher than dehydration in the active group (P<0.001), and was 1.7-2.2 times lower than dehydration in I3 and I5 (P<0.05), with no significant difference in I10. DEQLF may be used to clinically evaluate early caries activity, and longitudnally monitor changes in lesion activity.

scholarly journals Microleakage analysis of dental caries lesions sealed with flow resin and compared to microhybrid resin restorations in dentin

2018 ◽  
Vol 66 (2) ◽  
pp. 141-146
Author(s):  
Amanda de Albuquerque VASCONCELOS ◽  
Juliana Tietbohl de Almeida REIS ◽  
Bianca Fiorentin MOURA ◽  
Daniela Cavalcante GIRÃO ◽  
José Carlos Pettorossi IMPARATO ◽  
...  
Keyword(s):  
Composite Resin ◽  
Descriptive Analysis ◽  
Resin Flow ◽  
Occlusal Caries ◽  
Dye Penetration ◽  
Group I ◽  
Significant Difference ◽  
Distal Direction ◽  
Caries Lesions

ABSTRACT Objective: To evaluate the sealing of cavities of dentinal occlusal caries lesions, reproduced in vitro, with flow resin compared to cavity restorations presenting healthy dentin using microhybrid composite resin. Methods: The sample consisted of 27 healthy deciduous molars where cavities of approximately 2 mm in the fossa region were performed and occlusal cleft of each tooth were sealed, impermeabilization was performed and the sample was randomly divided into 2 groups: group I underwent cariogenic challenge and occlusal sealing with resin flow. The teeth of group II were restored with microhybrid composite resin. The teeth were immersed in 5% methylene blue for 8 hours at 37° C and washed until all the dye was removed from the surface. The teeth were sectioned in the mesio-distal direction. The penetration of the dye was evaluated: 0- no penetration; 1- dye penetration up to 1/3 of the restoration; 2- dye penetration up to 2/3 of the restoration depth; 3 - penetration of dye into the pulp wall. The results were analyzed by the Biostat 4.0 program. Descriptive analysis and the mode among the examiners submitted to the Mann-Whitney test. Results: There was no significant difference in microleakage between restoration performed in healthy dentin with microhybrid composite resin or maintenance of infected dentin in primary teeth sealed with resin flow (p = 0.6035). Conclusion: It was concluded that the marginal infiltration of primary molars sealed with microhybrid composite resin and resin flow was not influenced by the removal -or not -of the carious tissue or the material used.

scholarly journals A Comparison of the Accuracy of Digital and Conventional Radiography in the Diagnosis of Recurrent Caries

2010 ◽  
Vol 11 (6) ◽  
pp. 25-32 ◽  
Author(s):  
Najmeh Anbiaee ◽  
Anousheh Rashed Mohassel ◽  
Mahrokh Imanimoghaddam ◽  
Seyed Mostafa Moazzami
Keyword(s):  
Sensitivity And Specificity ◽  
Digital Radiography ◽  
Conventional Radiography ◽  
Class Ii ◽  
Laboratory Research ◽  
Digital Radiographs ◽  
Carious Lesions ◽  
Significant Difference ◽  
Line Angle ◽  
Caries Lesions

Abstract Aim The purpose of this laboratory research was to compare the accuracy of digital and conventional bitewing radiographs in the diagnosis of recurrent caries under class II amalgam restorations. Methods and Materials This study involved 82 posterior intact teeth in which class II amalgam boxes were prepared. Carious lesions were simulated in half of the proximal boxes in the intersection between the facial or lingual wall and the gingival floor or midway between the facial and lingual walls. The other half of each tooth specimen served as a control. The prepared boxes were then restored with a Tytin FC (Kerr, USA) amalgam. The teeth were radiographed in the bucco-lingual direction to obtain images comparable to bitewing. Digital radiographs made with an intraoral CCD sensor and conventional radiography with dental E film were used. Three expert observers evaluated both types of images for the diagnosis of recurrent caries. Results Sensitivity and specificity values for direct digital radiography were 73 and 95 percent at the buccal and lingual line angles, respectively, and 29 and 90 percent at the mid-gingival floor, respectively. These corresponding values for conventional radiography were respectively 63 and 93 percent at the buccal line angle, 61 and 93 percent at the lingual line angle, and 44 and 95 percent at the mid-gingival floor. The total sensitivity and specificity values were 58 and 93 percent for digital radiography and 56 and 93 percent for conventional radiography. The overall accuracy was 76 percent for digital and 75 percent for conventional radiography. No significant difference in specificity or sensitivity was found between the digital and conventional radiography (p=0.104). Separately, no significant difference was seen between the buccal line angle and the mid-gingival floor, but a significant difference was seen between the two methods in the lingual line angle (p=0.004). Conclusion The digital and conventional bitewing radiographs had similar diagnostic accuracy for the diagnosis of recurrent caries. Lesions located at the buccal or lingual line angle were more easily detected than those at the mid-gingival region. Clinical Significance Although there was no significant difference between digital and conventional radiography in the diagnosis of recurrent caries, digital radiography requires less ionizing radiation, making this method of imaging suggested for routine dental practice. Citation Anbiaee N, Rashed A, Imanimoghaddam M, Moazzami SM. A Comparison of the Accuracy of Digital and Conventional Radiography in the Diagnosis of Recurrent Caries. J Contemp Dent Pract [Internet]. 2010 December; 11(6):025-032. Available from: http://www.thejcdp.com/journal/ view/volume11-issue6-anbiaee

scholarly journals Effectiveness of Ozone with or without the Additional Use of Remineralizing Solution on Non-Cavitated Fissure Carious Lesions in Permanent Molars

2011 ◽  
Vol 05 (04) ◽  
pp. 393-399 ◽  
Author(s):  
Didem Atabek ◽  
Nurhan Oztas
Keyword(s):  
Wilcoxon Test ◽  
Clinical Severity ◽  
Ozone Treatment ◽  
Carious Lesions ◽  
Mandibular Molar ◽  
Significant Difference ◽  
Molar Teeth ◽  
Permanent Molars ◽  
Dependent Samples ◽  
Caries Lesions

ABSTRACTObjectives: The aim of this study was to evaluate the efficiency of ozone alone and with a re-mineralizing solution following application on initial pit and fissure caries lesions in permanent molars. Methods: Forty children (9-12 years) having non-cavitated fissure caries lesions on bilateral 40 first permanent mandibular molar teeth were participated in the study. Patients were randomly allocated to 2 experimental groups consisting of 20 subjects. In the first group, ozone was applied once for 40 seconds to the assigned test teeth of each pair. In the second group, ozone was applied once for 40 seconds to the assigned test teeth of each pair with the use of re-mineralizing solution. Progression or improvement of the caries was assessed at baseline, immediately after treatment and at 1-,2-,3-, and 6 month follow-up by comparing the DIAGNOdent values, Clinical Severity Indexes, Oral Hygiene Scores. The results were analyzed statistically by using the Wilcoxon-Test for dependent samples in each group. When comparing different test groups (control and experimental groups) the Friedman S test followed by the Mann-Whitney U test was used. Results: A statistically significant difference was found between all of the control and experimental test lesions in each group (P<.001). However, there was no statistically significant difference between the ozone treated groups and those using the additional re-mineralizing solution (P>.001). Conclusions: Ozone treatment either alone or combined with a re-mineralizing solution was found to be effective for remineralization of initial fissure caries lesions. (Eur J Dent 2011;5:393-399)

scholarly journals A Novel Fluoride Containing Bioactive Glass Paste is Capable of Re-Mineralizing Early Caries Lesions

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1636 ◽  
Author(s):  
Ahmed Bakry ◽  
Mona Abbassy ◽  
Hanin Alharkan ◽  
Sara Basuhail ◽  
Khalil Al-Ghamdi ◽  
...  
Keyword(s):  
White Spot ◽  
White Spot Lesions ◽  
Mineral Density ◽  
Treatment Area ◽  
Cross Sectional ◽  
Significant Difference ◽  
Glass Paste ◽  
Early Caries ◽  
Caries Lesions ◽  
One Way Anova

White-spot-lesions (WSL) are a common complication associated with orthodontic treatment. In the current study, the remineralization efficacy of a BiominF® paste was compared to the efficacy of a fluoride gel. Methods: Orthodontic brackets were bonded to 60 human premolars buccal surfaces, which were covered with varnish, except a small treatment area (3 mm2). All specimens were challenged by a demineralization solution for 4 days. Specimens were assigned into 4 groups: BiominF® paste, Fluoride (4-min application), fluoride (twenty four hours application), and the control (n = 15). After cross-sectioning, enamel slabs having a thickness of approximately 100–120 μm were obtained. A TMR (Transverse Micro Radiography) technique was used to observe the sub-surface enamel lesions’ depth and mineral density, and their response to the remineralization protocols. One way ANOVA was used to analyze the results (α = 0.05). The top and the cross-sectional surfaces were observed using SEM/EDS. Results: Specimens treated with BiominF® paste showed significant decrease in delta z values, however lesion depth showed no significant difference when compared to the other three groups (p < 0.05). SEM/EDS observation showed the formation of crystal like structures on top of enamel demineralized surfaces, when treated with BiominF® paste. In conclusion BiominF® paste can be considered an effective remineralizing agent for white spot Lesions.

scholarly journals Effect of bleaching on sound enamel and with early artificial caries lesions using confocal laser microscopy

2012 ◽  
Vol 23 (2) ◽  
pp. 110-115 ◽  
Author(s):  
Sandrine Bittencourt Berger ◽  
Sabrina Pavan ◽  
Paulo Henrique dos Santos ◽  
Marcelo Giannini ◽  
Ana Karina B. Bedran-Russo
Keyword(s):  
Hydrogen Peroxide ◽  
Calcium Phosphate ◽  
Fluorescence Intensity ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Significant Difference ◽  
Ph Cycling ◽  
Bovine Enamel ◽  
Caries Lesions

The aim of this study was to evaluate effect of bleaching agents on sound enamel (SE) and enamel with early artificial caries lesions (CL) using confocal laser scanning microscopy (CLSM). Eighty blocks (4 x 5 x 5 mm) of bovine enamel were used and half of them were submitted to a pH cycling model to induce CL. Eight experimental groups were obtained from the treatments and mineralization level of the enamel (SE or CL) (n=10). SE groups: G1 - unbleached (control); G2 - 4% hydrogen peroxide (4 HP); G3 - 4 HP containing 0.05% Ca (Ca); G4 - 7.5% hydrogen peroxide (7.5 HP) containing amorphous calcium phosphate (ACP). CL groups: G5 - unbleached; G6 - 4 HP; G7 - 4 HP containing Ca; G8 - 7.5 HP ACP. G2, G3, G6, G7 were treated with the bleaching agents for 8 h/day during 14 days, while G4 and G8 were exposed to the bleaching agents for 30 min twice a day during 14 days. The enamel blocks were stained with 0.1 mM rhodamine B solution and the demineralization was quantified using fluorescence intensity detected by CLSM. Data were analyzed using ANOVA and Fisher’s tests (α=0.05). For the SE groups, the bleaching treatments increased significantly the demineralization area when compared with the unbleached group. In the CL groups, no statistically significant difference was observed (p>0.05).The addition of ACP or Ca in the composition of the whitening products did not overcome the effects caused by bleaching treatments on SE and neither was able to promote remineralization of CL.

scholarly journals Resin Infiltration into Differentially Extended Experimental Carious Lesions

2014 ◽  
Vol 8 (1) ◽  
pp. 251-256 ◽  
Author(s):  
Wolfgang H Arnold ◽  
Lena Bachstaedter ◽  
Korbinian Benz ◽  
Ella A Naumova
Keyword(s):  
Experimental Studies ◽  
Sodium Fluorescein ◽  
Time Interval ◽  
Element Analysis ◽  
Resin Infiltration ◽  
Initial Caries ◽  
Carious Lesions ◽  
Fluo Rescence ◽  
Novel Method ◽  
Caries Lesions

Resin infiltration of initial caries lesions is a novel method of caries therapy. However, it has some limitations. Therefore, further experimental studies are needed to improve resin infiltration. It was the aim of this investigation to study resin infiltra-tion into different experimental carious lesions. Caries-free extracted human molars and premolars were demineralized for 3, 6, 9 and 12 days and infiltrated with resin. Prior to infiltration, the teeth were incubated with sodium fluorescein. After em-bedding, serial sections were cut through the experimental lesions, and the penetration of the resin was measured with fluo-rescence microscopy. Two infiltrated teeth from each time interval were not embedded and cut. Infiltration of the resin was then studied with EDS element analysis. The results showed that with increasing demineralization time, the lesion expansion was also increasing, and the resin infiltration was always almost complete. From these results it can be concluded that artifi-cial standardized caries-like lesions are suitable for experimental studies of resin infiltration.

Remineralization Potential of Mints Containing Bioactive Agents in Artificially Induced Root Caries

2018 ◽  
Vol 52 (4) ◽  
pp. 331-338 ◽  
Author(s):  
Camila A. Zamperini ◽  
Ana K. Bedran-Russo
Keyword(s):  
Fluorescence Intensity ◽  
Negative Control ◽  
Root Caries ◽  
Mineral Density ◽  
Solution Ph ◽  
Bacterial Collagenase ◽  
Significant Difference ◽  
Bioactive Agents ◽  
Tea Extract ◽  
Caries Lesions

This study investigated the remineralization effect of experimental mint formulations containing bioactive agents (xylitol; green tea extract, GT; and amorphous calcium phosphate, ACP) in the progression of artificially induced root caries. Root caries lesions were induced by demineralization solution (pH 4.6; 96 h; 37°C). The lesions were treated with mint A, mint B, mint C, xylitol, GT, ACP, or remineralization solution (RS; negative control). Specimens were pH-cycled through treatments (5×/day; 3 min) and 6 cycles of acidic (pH 5.0; 30 min) and neutral (pH 7.0; 10 min) buffers for 8 days. Bacterial collagenase (Clostridium histolyticum) was used overnight to simulate proteolytic challenge. Caries depth and porosity as well as mineral density were estimated using fluorescence microscopy (n = 15) and microcomputed tomography (n = 6). Analysis of variance (ANOVA, α = 0.05) showed no statistically significant difference in caries depth among all groups (p = 0.172). The highest fluorescence intensity decrease was observed for GT followed by mint C, with no significant difference between them (p = 0.868). There were significant differences among GT and mints A, B, and C when compared to RS (p < 0.001). No statistically significant differences in fluorescence intensity were observed among ACP, xylitol, and RS (p > 0.05). The mineral density of the lesions in GT, mints A, B, and C, and ACP was statistically similar (p > 0.05) and significantly higher than that in RS (p < 0.05). No significant difference was observed between xylitol and RS (p = 0.728). The experimental mints showed remineralization action on artificial root caries, and GT was found to be the main active ingredient in the investigated formulations.

Near-infrared fluorescence imaging with indocyanine green for quantification of changes in tissue perfusion following revascularization

Vascular ◽  
2021 ◽  
pp. 170853812110328
Author(s):  
Pim Van den Hoven ◽  
Floris S Weller ◽  
Merel Van De Bent ◽  
Lauren N Goncalves ◽  
Melissa Ruig ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Fluorescence Intensity ◽  
Near Infrared ◽  
Tissue Perfusion ◽  
Control Group ◽  
Maximum Slope ◽  
Nir Fluorescence ◽  
Significant Difference ◽  
Revascularization Procedures

Objectives Current diagnostic modalities for patients with peripheral artery disease (PAD) mainly focus on the macrovascular level. For assessment of tissue perfusion, near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) seems promising. In this prospective cohort study, ICG NIR fluorescence imaging was performed pre- and post-revascularization to assess changes in foot perfusion. Methods ICG NIR fluorescence imaging was performed in 36 patients with PAD pre- and post-intervention. After intravenous bolus injection of 0.1 mg/kg ICG, the camera registered the NIR fluorescence intensity over time on the dorsum of the feet for 15 min using the Quest Spectrum Platform®. Time-intensity curves were plotted for three regions of interest (ROI): (1) the dorsum of the foot, (2) the forefoot, and (3) the hallux. Time-intensity curves were normalized for maximum fluorescence intensity. Extracted parameters were the maximum slope, area under the curve (AUC) for the ingress, and the AUC for the egress. The non-treated contralateral leg was used as a control group. Results Successful revascularization was performed in 32 patients. There was a significant increase for the maximum slope and AUC egress in all three ROIs. The most significant difference was seen for the maximum slope in ROI 3 (3.7%/s to 6.6%/s, p < 0.001). In the control group, no significant differences were seen for the maximum slope and AUC egress in all ROIs. Conclusions This study shows the potential of ICG NIR fluorescence imaging in assessing the effect of revascularization procedures on foot perfusion. Future studies should focus on the use of this technique in predicting favorable outcome of revascularization procedures.

scholarly journals Remineralization of natural early caries lesions in vitro by P11-4 monitored with photothermal radiometry and luminescence

2017 ◽  
Vol 8 (4) ◽  
pp. e12257 ◽  
Author(s):  
Joshua D. Silvertown ◽  
Bonny P. Y. Wong ◽  
Koneswaran S. Sivagurunathan ◽  
Stephen H. Abrams ◽  
Jennifer Kirkham ◽  
...  
Keyword(s):  
Photothermal Radiometry ◽  
Early Caries ◽  
Caries Lesions
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