Comprehensive Analysis of a ceRNA Network Reveals Potential Prognostic lncRNAs Involved in Progression of Bladder Cancer
Abstract Background: The aberrant expression of long non-coding RNAs (lncRNAs) has attracted more and more attention in the biological field of bladder cancer(BC). We aim to construct a competing endogenous RNA(ceRNA) network reveals potential prognostic lncRNAs involved in progression of BC.Results: Expression profiles of messenger RNA(mRNA), micro RNA(miRNA) and lncRNA of 397 BC samples and 19 non-tumor tissues were downloaded from The Cancer Genome Atlas(TCGA) database. Patients with BC were randomly divided into training group (n=198) and validation group (n=199). Then, 130 lncRNAs, 159 miRNAs and 2,048 mRNAs were identified as differentially expressed genes (DEGs, |logFC|>1, FDR<0.01) related to BC progression. Nextly, we constructed an BC associated deregulated competing endogenous RNA(ceRNA) network with 70 lncRNAs, 30 miRNAs, and 62 mRNAs involved in. Subsequently, a seven-lncRNA signature was constructed by establishing a LASSO Cox model with 13 lncRNAs associated with survival from the ceRNA network. This signature can well distinguish high-risk patients from low-risk patients in training group and verification group. Furthermore, we combined the risk score model with other clinical fictures to estimate the ability of survival prediction. The result suggested that the risk score can be selected as an independent prognostic factor for overall survival(OS) rate. Conclusion: In this study, we construct a ceRNA network related to progression of BC and established an seven-lncRNA signature, which was a candidate prognostic biomarker for prognostic prediction of BC patients .