Assessment of Sodium Thiosulfate Neutralizing Effect on Micro-hardness of Dentin Treated With Sodium Hypochlorite
Abstract Background The aim of this study is to evaluate the ability of sodium thiosulfate (STS) to reverse the adverse effect of sodium hypochlorite (NaOCl) on dentin microhardness. Methods Fifty single-rooted teeth were decoronated and longitudinally sectioned. The samples were divided into a control and four sample groups (n = 20): Normal saline for 15 min as the control group, G1: 2.5% NaOCl for 15 min without an incubation period, G2: 2.5% NaOCl for 15 min, G3: 2.5% NaOCl for 15 min irrigated with normal saline followed by 5% Na2S2O3 for 10 min, G4: Normal saline for 15 min followed by 5% Na2S2O3 for 10 min. All groups except G1 were incubated for 1 week. The microhardness measurements were determined using the Vickers micro-hardness test. Data were analyzed using the Kruskal-Wallis test for pairwise comparisons. A p-value < 0.05 was considered significant. Results All groups showed a significant decrease in the micro-hardness value compared with the control group. NaOCl for 1 week (G2) reduced the micro-hardness of dentine compared with samples, tested immediately after immersion in NaOCl (G1) (p < 0.05). NaOCl alone (G2) or treated with Na2S2O3 (G3) resulted in a significant decrease in microhardness compared with the Na2S2O3 group (G4) (p < 0.05). Conclusions Sodium thiosulfate as a neutralizing agent could not prevent the microhardness downturn caused by sodium hypochlorite