scholarly journals Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop

2019 ◽  
Vol 20 (2) ◽  
pp. 243 ◽  
Author(s):  
Fei Gao ◽  
Pengju Ma ◽  
Yingxin Wu ◽  
Yijun Zhou ◽  
Genfa Zhang
Keyword(s):  
Superoxide Dismutase ◽  
Stress Response ◽  
Cold Stress ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Soluble Sugars ◽  
Pcr Analysis ◽  
Ros Scavenging ◽  
Quantitative Proteomic Analysis ◽  
Woody Crop

Jojoba (Simmondsia chinensis) is a semi-arid, oil-producing industrial crop that have been widely cultivated in tropical arid region. Low temperature is one of the major environmental stress that impair jojoba’s growth, development and yield and limit introduction of jojoba in the vast temperate arid areas. To get insight into the molecular mechanisms of the cold stress response of jojoba, a combined physiological and quantitative proteomic analysis was conducted. Under cold stress, the photosynthesis was repressed, the level of malondialdehyde (MDA), relative electrolyte leakage (REL), soluble sugars, superoxide dismutase (SOD) and phenylalanine ammonia-lyase (PAL) were increased in jojoba leaves. Of the 2821 proteins whose abundance were determined, a total of 109 differentially accumulated proteins (DAPs) were found and quantitative real time PCR (qRT-PCR) analysis of the coding genes for 7 randomly selected DAPs were performed for validation. The identified DAPs were involved in various physiological processes. Functional classification analysis revealed that photosynthesis, adjustment of cytoskeleton and cell wall, lipid metabolism and transport, reactive oxygen species (ROS) scavenging and carbohydrate metabolism were closely associated with the cold stress response. Some cold-induced proteins, such as cold-regulated 47 (COR47), staurosporin and temperature sensitive 3-like a (STT3a), phytyl ester synthase 1 (PES1) and copper/zinc superoxide dismutase 1, might play important roles in cold acclimation in jojoba seedlings. Our work provided important data to understand the plant response to the cold stress in tropical woody crops.

scholarly journals Label-Free Quantitative Proteomic Analysis of the Global Response to Indole-3-Acetic Acid in Newly Isolated Pseudomonas sp. Strain LY1

2021 ◽  
Vol 12 ◽  
Author(s):  
Shuxue Zhao ◽  
Xi Chen ◽  
Qianshu Sun ◽  
Fei Wang ◽  
Chunhui Hu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Proteomic Analysis ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Degradation Mechanism ◽  
Label Free ◽  
Ros Scavenging ◽  
Protein Repair ◽  
Quantitative Proteomic Analysis ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid (IAA), known as a common plant hormone, is one of the most distributed indole derivatives in the environment, but the degradation mechanism and cellular response network to IAA degradation are still not very clear. The objective of this study was to elucidate the molecular mechanisms of IAA degradation at the protein level by a newly isolated strain Pseudomonas sp. LY1. Label-free quantitative proteomic analysis of strain LY1 cultivated with IAA or citrate/NH4Cl was applied. A total of 2,604 proteins were identified, and 227 proteins have differential abundances in the presence of IAA, including 97 highly abundant proteins and 130 less abundant proteins. Based on the proteomic analysis an IAA degrading (iad) gene cluster in strain LY1 containing IAA transformation genes (organized as iadHABICDEFG), genes of the β-ketoadipate pathway for catechol and protocatechuate degradation (catBCA and pcaABCDEF) were identified. The iadA, iadB, and iadE-disrupted mutants lost the ability to grow on IAA, which confirmed the role of the iad cluster in IAA degradation. Degradation intermediates were analyzed by HPLC, LC-MS, and GC-MS analysis. Proteomic analysis and identified products suggested that multiple degradation pathways existed in strain LY1. IAA was initially transformed to dioxindole-3-acetic acid, which was further transformed to isatin. Isatin was then transformed to isatinic acid or catechol. An in-depth data analysis suggested oxidative stress in strain LY1 during IAA degradation, and the abundance of a series of proteins was upregulated to respond to the stress, including reaction oxygen species (ROS) scavenging, protein repair, fatty acid synthesis, RNA protection, signal transduction, chemotaxis, and several membrane transporters. The findings firstly explained the adaptation mechanism of bacteria to IAA degradation.

scholarly journals iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

2020 ◽  
Author(s):  
Jing Wang ◽  
Chengliang Liang ◽  
Sha Yang ◽  
Jingshuang Song ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Tolerance ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Negative Impact ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
Heat Stress Response ◽  
Quantitative Proteomic Analysis

Abstract Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

scholarly journals iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11509
Author(s):  
Jing Wang ◽  
Chengliang Liang ◽  
Sha Yang ◽  
Jingshuang Song ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Proteomic Analysis ◽  
Negative Impact ◽  
Bioinformatic Analysis ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
Ros Scavenging ◽  
Heat Stress Response ◽  
Quantitative Proteomic Analysis

Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

scholarly journals Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1706
Author(s):  
Mei Yang ◽  
Xinhang Duan ◽  
Zhaoyu Wang ◽  
Hang Yin ◽  
Junrui Zang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Cold Stress ◽  
Medicago Sativa ◽  
Transgenic Tobacco ◽  
Soluble Sugars ◽  
Ros Scavenging ◽  
Osmotic Homeostasis ◽  
Voltage Dependent ◽  
Stress Responsive Genes

Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of VDACs in plants are still poorly understood. Here, a novel VDAC gene was isolated and identified from alfalfa (Medicago sativa L.). MsVDAC localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of MsVDAC in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O2− content increased. Stress-responsive genes including LTP1, ERD10B and Hxk3 were upregulated in the transgenic plants under cold and drought stress. However, GAPC, CBL1, BI-1, Cu/ZnSOD and MnSOD were upregulated only in the transgenic tobacco plants under cold stress, and GAPC, CBL1, and BI-1 were downregulated under drought stress. These results suggest that MsVDAC provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via MsVDAC may be mainly due to osmotic homeostasis and stress-responsive genes.

Proteomic analysis of the cold stress response in the moss, Physcomitrella patens

PROTEOMICS ◽  
2009 ◽  
Vol 9 (19) ◽  
pp. 4529-4538 ◽  
Author(s):  
Xiaoqin Wang ◽  
Pingfang Yang ◽  
Xiaofeng Zhang ◽  
Yinong Xu ◽  
Tingyun Kuang ◽  
...  
Keyword(s):  
Stress Response ◽  
Cold Stress ◽  
Proteomic Analysis ◽  
Physcomitrella Patens

scholarly journals Comparative Proteomic Analysis of the Stolon Cold Stress Response between the C4 Perennial Grass Species Zoysia japonica and Zoysia metrella

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75705 ◽  
Author(s):  
Jiping Xuan ◽  
Yufeng Song ◽  
Hongxiao Zhang ◽  
Jianxiu Liu ◽  
Zhongren Guo ◽  
...  
Keyword(s):  
Stress Response ◽  
Cold Stress ◽  
Proteomic Analysis ◽  
Perennial Grass ◽  
Grass Species ◽  
Zoysia Japonica ◽  
Comparative Proteomic Analysis

scholarly journals iTRAQ-based quantitative proteome analysis insights into cold stress of Winter Rapeseed (Brassica rapa L.) grown in the field

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zaoxia Niu ◽  
Lijun Liu ◽  
Yuanyuan Pu ◽  
Li Ma ◽  
Junyan Wu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Brassica Rapa ◽  
Molecular Mechanisms ◽  
Glutathione Transferase ◽  
Soluble Sugars ◽  
Freezing Stress ◽  
Transferase Activity ◽  
Oilseed Crop ◽  
Winter Rapeseed ◽  
Collar Diameter

AbstractWinter rapeseed (Brassica rapa L.) is a major oilseed crop in Northern China, where its production was severely affected by chilling and freezing stress. However, not much is known about the role of differentially accumulated proteins (DAPs) during the chilling and freezing stress. In this study, isobaric tag for relative and absolute quantification (iTRAQ) technology was performed to identify DAPs under freezing stress. To explore the molecular mechanisms of cold stress tolerance at the cellular and protein levels, the morphological and physiological differences in the shoot apical meristem (SAM) of two winter rapeseed varieties, Longyou 7 (cold-tolerant) and Lenox (cold-sensitive), were explored in field-grown plants. Compared to Lenox, Longyou 7 had a lower SAM height and higher collar diameter. The level of malondialdehyde (MDA) and indole-3-acetic acid (IAA) content was also decreased. Simultaneously, the soluble sugars (SS) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, soluble protein (SP) content, and collar diameter were increased in Longyou 7 as compared to Lenox. A total of 6330 proteins were identified. Among this, 98, 107, 183 and 111 DAPs were expressed in L7 CK/Le CK, L7 d/Le d, Le d/Le CK and L7 d/L7 CK, respectively. Quantitative real-time PCR (RT-qPCR) analysis of the coding genes for seventeen randomly selected DAPs was performed for validation. These DAPs were identified based on gene ontology enrichment analysis, which revealed that glutathione transferase activity, carbohydrate-binding, glutathione binding, metabolic process, and IAA response were closely associated with the cold stress response. In addition, some cold-induced proteins, such as glutathione S-transferase phi 2(GSTF2), might play an essential role during cold acclimation in the SAM of Brassica rapa. The present study provides valuable information on the involvement of DAPs during cold stress responses in Brassica rapa L, and hence could be used for breeding experiments.

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