scholarly journals Sodium Humate And Glutamine Combined Supplementation Alleviate Diarrhea of Weaned Calves Via Alter Intestinal Microbiota And Metabolites

Author(s):  
Dong Wang ◽  
Yuanyi Du ◽  
Haotian Jia ◽  
Siqi Huang ◽  
Lei Lei ◽  
...  

Abstract Background: Weaning is one of the most stressful periods that cause gastrointestinal tract dysfunction and diarrhea in calves. HNa and Gln were reported to exert beneficial effects on promoting growth performance, decreasing diarrhea incidence, and modulating intestinal microbiota in animals. Therefore, this study investigated the effect of HNa and Gln combined supplementation on growth performance, diarrhea incidence, serum parameters, intestinal microbiome, and metabolites of weaned calves.Results: In Exp. 1, 40 calves at 51±3 days of age with similar body weight (66.82±4.31 kg) were randomly assigned to 4 treatments fed with a basal diet (NC group), and a basal diet supplemented with 100 mL of 1%, 3%, or 5% HNa+1% Gln, twice daily, respectively. In a 21-day trial, calves on the 5% HNa+1% Gln group had higher ADG and lower fecal score and diarrhea incidence than those in the control group (P < 0.05). In Exp. 2, 20 calves at 51±3 days of age with similar body weight (69.37±6.28 kg) were randomly assigned to 2 treatments fed with a basal diet (NC group) and a basal diet supplemented with 100 mL of 5% HNa+1% Gln, twice daily (H+G group, the dose was obtained from Exp. 1). In a 21-day trial, calves supplemented with HNa and Gln had higher final BW and ADG, serum IgG concentration and GSH-Px and T-AOC activities, but lower fecal score, diarrhea incidence, as well as serum DAO, D-lac, TNF-α, and MDA concentrations compared to NC group (P < 0.05). Analysis of intestinal microbiota indicated that supplemented with HNa and Gln significantly increased the abundance of phyla Firmicutes and genus of Bifidobacterium, Lactobacillus, Olsenella, Ruminiclostridium 9, Howardella, and uncultured organism, whereas the abundance of phyla Bacteroidetes, genus of Helicobacter and Lachnoclostridium were decreased as compared with NC group. Moreover, untargeted metabolomics analysis revealed that supplemented with HNa and Gln altered 18 metabolites and enriched 6 KEGG pathways (primary fatty acid biosynthesis) compared to the NC group. Conclusions: This study showed that combined supplemented with HNa and Gln could decrease diarrhea of weaned calves, which may be associated with improved intestinal microbial ecology and altered metabolism profile.

2012 ◽  
Vol 92 (3) ◽  
pp. 307-317 ◽  
Author(s):  
Duddoa Khonyoung ◽  
Koh-en Yamauchi ◽  
Tonglian Buwjoom ◽  
Buaream Maneewan ◽  
Narin Thongwittaya

Khonyoung, D., Yamauchi, K., Buwjoom, T., Maneewan, B. and Thongwittaya, N. 2012. Effects of dietary dried fermented ginger on growth performance, carcass quality, and intestinal histology of heat- stressed broilers. Can. J. Anim. Sci. 92: 307–317. To investigate the effects of a dried fermented ginger product (DFG) on growth performance, carcass quality, and intestinal histology of heat-stressed broiler, at 7 d of age, 200 male broilers were divided into 0% (control), 0.25% (0.25 S+F), 0.5% (0.5 S+F) and 1% (1 S+F) dietary DFG groups fed from 7 to 49 d of age (starter+finisher groups, S+F). Another group of chicks was fed the basal diet until 21 d of age. At 22 d of age, 150 birds from this group showing almost similar body weight were divided into 0.25% (0.25 F), 0.5% (0.5 F) and 1% (1 F) dietary DFG groups fed from 22 to 49 d of age (finisher groups, F); the control group was common for the S+F and F groups. The average temperature was around 31°C (23.2–38.6°C) throughout the trial. Compared with the control, the experimental DFG groups were not different in feed intake, body weight gain, feed efficiency and carcass quality. However, abdominal fat of the 1 S+F, 0.5 F, and 1 F groups decreased (P<0.05). Cell mitosis numbers in the duodenum tended to increase in the 1 S+F group (P<0.1). Epithelial cells on the jejunal villus apical surface of the control were mainly flat, and desquamations were observed. Desquamation was reduced with increasing DFG, and in the 1% DFG groups, many protuberant cells and cell clusters were present. The present results suggest that under high ambient temperature 1% dietary DFG from 7 to 49 d of age can decrease abdominal fat, and that jejunal villus apex was desquamated with heat stress, but desquamations were reduced with increasing DFG, and disappeared in 1% DFG groups.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 202-203
Author(s):  
Sudario Roberto Silva Junior ◽  
Maíra Resende ◽  
Rhuan F Chaves ◽  
Jéssica Aparecida Barbosa ◽  
Iana I M Ferreira ◽  
...  

Abstract Benzoic acid (BA) and essential oils (EO) can minimize growth performance losses due to the removal of antibiotics and change the intestinal health of weaned piglets. The objective of this study was to evaluate the effects of BA and EO on inflammatory response, diarrhea incidence, and growth performance of the nursery phase. One hundred and twenty barrows were weaned at 23 days (6.40 ± 0.53 kg) and assigned into 3 treatments (10 replicates) in randomized block design: basal diet without additives (NC), basal diet with 200 ppm of colistin sulphate (PC), and association of 0,3% benzoic acid and essential oil (BA+EO). The feed intake and body weight were recorded at 0 and 42 days. The feces were assessed daily (per animal) and graded as normal feces (no diarrhea) or liquid or pasty stools (presence of diarrhea). On days 1, 3, and 9, blood samples were collected (5 replicates) for white blood cells (WBC) counts. Growth performance was analyzed by MIXED procedure (SAS, 2009) and the Tukey test was used to compare the means (P &lt; 0.050). The WBC counts were analyzed by repeated-measures analysis of variance, by MIXED procedure. Diarrhea incidence was analyzed by GENMOD procedure (SAS, 2009). The BA+EO treatment showed a similar body weight (P = 0.014) and average daily gain (P = 0.012) than the PC group and lower feed conversion ratio (P = 0.037) compared to the NC group. The pigs of the BA+EO treatment had the lowest diarrhea incidence during the total period (P &lt; 0.001). The supplementation with BA+EO or antibiotics reduced the counts of total WBC (P = 0.008) and neutrophils (P = 0.003). In conclusion, supplementation with BA+EO reduces the inflammatory response and the incidence of diarrhea in the nursery phase, that may be related to the improvement in the FCR.


2013 ◽  
Vol 42 (1) ◽  
pp. 40-43 ◽  
Author(s):  
S Yesmin ◽  
ME Uddin ◽  
R Chacrabati ◽  
M Al-Mamun

The present study was conducted to evaluate the effect of different levels of methionine supplementation on feed intake, nutrient digestibility and growth performance of growing rabbit. Sixteen weaned crossbred New Zealand White (NZW) growing rabbits (30-35 d) were distributed into four treatment groups having four replications in each group using a Completely Randomized Design (CRD). Basal diet composed of green grass (dhal grass) and concentrate mixture which was offered ad libitum basis for 56 days period. Four levels of methionine such as 0.0% (control), 0.15%, 0.25%, and 0.35% were supplemented randomly to rabbits. Results showed that supplementation of methionine did not affect green grass intake. Cumulative as well as daily concentrate and DM intake were significantly (p<0.05) higher for all methionine groups than control group. Final body weight gain as well as daily, weekly and cumulative body weight gains were improved significantly with increasing level of methionine. It was found that methionine had significant (p<0.01) effect on digestibility of DM, CP, NFE and EE but CF digestibility did not differ significantly. Digestibility was improved with increasing the level of methionine. Feed conversion ratio also decreased significantly with methionine supplementation, and 0.25% methionine group showed the best performance among the four treatments. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15777 Bang. J. Anim. Sci. 2013. 42 (1): 40 43


2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Abdallah E. Metwally ◽  
Ahmed A. A. Abdel-Wareth ◽  
Ahmed A. Saleh ◽  
Shimaa A. Amer

Abstract Background The aim of this study is to investigate whether the energy matrix values of the nonstarch polysaccharide- (NSP-) degrading enzymes, bioemulsifier (LYSOFORTE®), guanidinoacetic acid (CreAMINO®), or their combinations could be summed. The effects of these additives on the growth performance, carcass traits, and economic value of the broiler chicken diets were evaluated. A total of 525-one-day-old Ross chicks with an initial body weight of 42.96 ± 0.87 g were haphazardly allocated into seven groups with five replicates. The seven experimental treatments are as follows: (1) basal diet with no additives (breeder recommendation), which is the control group, (2) basal diet minus 100 kcal/kg supplemented with 0.02% NSP-degrading enzymes (NSP), (3) basal diet minus 50 kcal/kg supplemented with 0.025% emulsifier (LYSOFORTE®), (4) basal diet minus 50 kcal/kg supplemented with 0.06% guanidinoacetic acid (CreAMINO®), (5) basal diet minus 150 kcal/kg supplemented with a mixture of NSP and LYSOFORTE® (NSPL), (6) basal diet minus 100 kcal/kg supplemented with a mixture of NSP and CreAMINO® (NSPC), and (7) basal diet minus 200 kcal/kg supplemented with a mixture of NSP, LYSO, and CreAMINO® (NSPLC). The experiment lasted for 35 days. Results It was found that the final body weight, body weight gain, and relative growth rate were significantly higher in birds fed diets supplemented with NSPL, NSPC, CreAMINO, and LYSO with the reduced energy matrix value. The overall feed conversion ratio was significantly improved due to the supplementation of NSPC, CreAMINO, NSPL, and LYSO with the reduced energy matrix value compared to the control group. Moreover, no significant effect on the carcass criteria was observed by the different treatments. As a result of the dietary supplementation with NSPL, NSPC, CreAMINO®, and LYSO with the reduced energy matrix value, the net profit, total return, economic efficiency, and performance index were increased and the cost of feed per kg of body weight gain was decreased. Conclusion The energy matrix value of NSPL, NSPC, CreAMINO®, and LYSOFORTE could be established in the diets of broiler chickens to improve the growth performance and economic efficiency.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 405-407
Author(s):  
Koo Deokho ◽  
Mohankumar Thamaraikannan ◽  
Madesh Muniyappan ◽  
Song Junho ◽  
Ahn Je Min ◽  
...  

Abstract Minerals enhance the digestive and bio-synthesis process and growth of animals. Nano-minerals are considered to be more efficient in growth, immunomodulation, bactericidal effects than regular products. Also, they are needed in a lower dose. Sulfur is an essential part of many enzymes and antioxidant molecules like glutathione and thioredoxin. Some sulfur containing compounds can efficiently form a line of defense against reactive oxygen and nitrogen species. This study aimed to evaluate the effects of Detoxified nano-Sulfur Dispersion (DSD) on growth performance, fecal score, fecal microbial, gas emissions, blood profile, nutrient digestibility and meat quality in finishing pigs. A total of 160 pigs with an initial body weight of 54.90 ± 5.10 kg were randomly assigned to 2 treatments comprising of basal diet and basal diet with 10ppm DSD. All data were statically analyzed by T-test using the SAS program as a randomized complete block design, with the pen serving as an experimental unit. During the 10-week trial, there were no differences (P &gt; 0.05) in body weight (BW), average daily gain (ADG), average daily feed intake (ADFI) and gain to feed ratio (G:F) between the control and DSD groups. Also, the fecal score, fecal microbiota, gas emission were not affected (P &gt; 0.05) by DSD diet. Dietary inclusion of DSD tended (P &lt; 0.10) to increase water holding capacity and decrease cooking loss and drip loss. At week 5, serum concentrations of glucose, calcium, total cholesterol, high-density level were increased, and triglyceride concentration was significantly (P &lt; 0.05) reduced in pigs fed with DSD than control diets. In summary, the inclusion of dietary DSD in the finishing pig diet improved serum Ca, glucose concentrations and lipid profiles. It also improved some meat quality traits, indicating its importance in improving the health status of animals.


2020 ◽  
Author(s):  
Sanjun Jin ◽  
Hao Yang ◽  
Fangju Liu ◽  
Xinping Diao ◽  
Qian Pang ◽  
...  

Abstract Background: With increasing of duck meat among consumers, the demand for growth performance and the meat quality health of ducks in increasing. Dietary curcumin altered the growth performance and meat quality. However, study on the effect of dietary curcumin on the growth performance, serum antioxidation and meat quality of ducks. This study invested effects of dietary curcumin given to ducks on the growth performance, antioxidation capacity in plasma, meat quality, lipid oxidation and the concentration of volatile compounds in duck breast muscle. A total of 600 healthy ducks with similar body weight were randomly allotted into 4 groups with 10 replicates per group and 15 ducks per replicate. Ducks were fed experimental diets which the curcumin supplemental levels were 0, 300, 400 and 500 mg curcumin/kg basic fed, respectively. The experiment lasted for 10 weeks. Results: Results showed that dietary supplementation of curcumin at levels 300, 400 and 500 mg/kg dietary (P < 0.05) improved the average final body weight. The ducks fed the basal diet without curcumin supplementation had the lowest FI (P > 0.05) and highest FCR (P > 0.05) among all ducks. Feeding ducks on diets supplemented with curcumin at each level significantly increased (P < 0.05) plasma activity of T-SOD and GSH-Px, and significantly reduced plasma MDA concentration compared to the control group. The dietary curcumin significantly increased the growth performance and the antioxidation capacity in plasma of ducks. Dietary curcumin improved the meat quality of ducks by significantly increasing pH45, pH24 (P < 0.05) and color of duck meat (P < 0.05), and significantly decreasing cooking loss (P < 0.05), shear force (P < 0.05), and the lipid oxidation of duck meat. 56 volatile compounds were identified and quantified. Interestingly, cantharidin among volatile compounds was firstly found in duck muscle, and only existed in the group of 500 mg curcumin/kg diet. Conclusions: In conclusion, the supplementation of duck diets with curcumin at 300, 400, and 500 mg/kg diet improved growth performance, antioxidant status and meat quality of ducks. The cantharidin was firstly found in duck breast muscle when the dietary curcumin inclusion of 500 mg/kg.


2021 ◽  
Vol 12 ◽  
Author(s):  
Simin Peng ◽  
Xin Wang ◽  
Yuyu Wang ◽  
Tuo Lv ◽  
Haohan Zhao ◽  
...  

Given the desirable results of using probiotics and enzyme preparations as feed supplements in poultry health, here, the effects of Bacillus and Non-starch Polysaccharase (NSPase) on the growth performance, serum antioxidant profiles, and gut microbial communities of early stage ducks is investigated. A total of 400 Zhijiang ducks (of similar body weight and 1 day age) was selected and randomly divided into four groups. The feeding period was 28 days. Each group contained 10 replicates of 10 birds. Control group (I) was fed with basal diet, while treatment groups II to IV were fed, respectively, with 150 mg/kg NSPases, 25 mg/kg Bacillus probiotics, and 150 mg/kg NSPases + 25 mg/kg Bacillus probiotics in their basal diet. The results demonstrated that dietary Bacillus (25 mg/kg) increased average final weight, average daily gain (ADG), and decreased the malonaldehyde (MDA) in birds (P &lt; 0.05). Dietary Bacillus (25 mg/kg) and NSPases + Bacillus (150 mg/kg + 25 mg/kg) presented much higher glutathione (GSH) and activities of superoxide dismutase (SOD) in birds (P &lt; 0.05). Additionally, as revealed by β-diversity indices and analysis of similarities, dietary NSPases + Bacillus could affect the ileum microbial abundances and diversities at the genera level (P &lt; 0.05), but it had no effect on the caecal microbiota. Also, 16S rRNA sequencing revealed that dietary Bacillus and NSPases + Bacillus increased the populations of Ruminococcaceae genera in the cecum (P &lt; 0.05), and S24-7_group and Lactobacillus genera in the ileum (P &lt; 0.05). However, dietary NSPases and Bacillus alone and in combination could significantly decrease the content of Bacteroides in the ileum (P &lt; 0.05). According to Spearman correlation analysis, 7 ilea bacterial microbiomes (S24-7 group, Lactobacillus, Subgroup 2, Subgroup 1, Kitasatospora, Candidatus Solibacter, and Akkermansia) were positively correlated with SOD (P &lt; 0.05). In conclusion, Bacillus (25 mg/kg) and NSPases (150 mg/kg) included in the diet could efficiently enhance the growth performance by altered gut microbiota composition at the genera level and antioxidant indices of ducks.


Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 123
Author(s):  
Dong Wang ◽  
Zhendong You ◽  
Yuanyi Du ◽  
Duo Zheng ◽  
Haotian Jia ◽  
...  

This study aimed to evaluate the effects of the administration of sodium humate (NaH) on the growth performance, diarrhea incidence, and fecal microflora of pre-weaned Holstein calves. In a 53-day experiment, forty healthy newborn female calves were randomly allocated to the following four treatment groups: (1) control (basal diet); (2) 1-gram NaH (basal diet extra orally supplemented with 1 g of NaH dissolved in 100 mL of milk or milk replacer daily); (3) 3-gram NaH (basal diet extra orally supplemented with 3 g of NaH dissolved in 100 mL of milk or milk replacer daily); and (4) 5-gram NaH (basal diet extra orally supplemented with 5 g of NaH dissolved in 100 mL of milk or milk replacer daily). NaH was mixed with milk (d 2–20) or milk replacer (d 21–53). Calves in the 5-gram NaH group had a higher ADG during d 1 to 21 and d 21 to 53 than the other groups did (p < 0.05). Fecal scores and diarrheal incidence were significantly lower in the 3-gram and 5-gram NaH groups than the 1-gram NaH and control groups during d 1 to 20 (p < 0.05). The serum IgA, IgG and IL-4 concentrations, and T-SOD and T-AOC activities were higher, and the serum IL-6, TNF-α, D-lactic acid, and MDA concentrations were lower in the 5-gram NaH group than the control group (p < 0.05). Furthermore, NaH supplementation increased the abundances of Bifidobacterium and Lactobacillus but decreased the abundance of Escherichia coli in feces (p < 0.05). These encouraging findings indicated that supplementation with 5 g of NaH effectively improved the immune status, antioxidant capacity, and intestinal beneficial bacteria, and further improved the growth performance and reduced the diarrhea incidence of the pre-weaned dairy calves.


Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1005 ◽  
Author(s):  
Hongyu Zhang ◽  
Haibo Wang ◽  
Kun Hu ◽  
Liting Jiao ◽  
Mingjun Zhao ◽  
...  

The purpose of this study is to investigate the effect of probiotics L. casei YYL3 (Lc) and L. plantarum YYL5 (Lp) on growth performance, innate immunity, disease resistance and intestinal microbiota of channel catfish. A total of 252 catfish (67.20 ± 1.46 g) were randomly divided into 3 groups which were fed with basal diet, Lc-added (3.0 × 108 cfu/g) or Lp-added (3.0 × 108 cfu/g) diets, respectively. After 4 weeks of feeding, Lc significantly enhanced the growth and feed utilization of channel catfish compared with the control group (CG). Following that, the catfish were challenged with an intraperitoneal injection of 200 μL of the pathogenic E.ictaluri (2.0 × 106 cfu/mL), the relative percent survival of Lc and Lp were 38.28% and 12.76%, respectively. High-throughput sequencing indicated Lc and Lp reduced the alpha diversity of the intestinal microbiota in channel catfish. Lactobacillus were overwhelming in the guts during probiotics treatment, but almost vanished away after 2 weeks post-cessation of probiotics administration. Compared to CG, Lc and Lp resulted in an increased abundance of Pseudomonas and decreased amount of Aeromonas. Functional analysis revealed that Lc treatment upregulated the relative abundance of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including lipid metabolism, metabolism of other amino acids, metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism, and nucleotide metabolism. Combined, our data revealed that Lc, as a feed additive at 3.0 × 108 cfu/g, could promote the growth performance, disease resistance and dramatically change the composition of intestinal microbiota of channel catfish.


Author(s):  
Thamaraikannan Mohankumar ◽  
In Sun Park ◽  
In Ho Kim

This study is to evaluate the effects of mineral Detoxified nano-Sulfur Dispersion (DSD) on growth performance, fecal score, fecal microbial, gas emissions, blood profile, nutrient digestibility and meat quality in finishing pigs. A total of 160 pigs with an initial body weight of 54.90 ± 5.10 kg were randomly assigned to 2 treatments include basal diet and basal diet with 10ppm DSD. During 10 week trial there were no differences in body weight (BW), average daily gain (ADG), average daily feed intake (ADFI) and gain to feed ratio (G:F) between the control and DSD groups. Also, the fecal score, fecal microbiota, gas emission was not affected by DSD diet. Dietary inclusion of DSD tended to increase water holding capacity and decrease cooking loss and drip loss. At week 5, serum concentrations of glucose, calcium, total cholesterol, high-density level were increased, and triglyceride concentration was reduced in pigs fed with DSD than control diets. In summary, the inclusion of dietary DSD in the finishing pig diet has improved serum Ca, glucose concentrations and lipid profiles as well as it improves some meat quality traits.


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