BRS1 mediates plant redox regulation and cold responses

2021 ◽  
Author(s):  
Dongzhi Zhang ◽  
Yuqian Zhao ◽  
Junzhe Wang ◽  
Peng Zhao ◽  
Shengbao Xu
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Isocitrate Dehydrogenase ◽  
Redox Regulation ◽  
Serine Carboxypeptidase ◽  
Developmental Processes ◽  
Brassinosteroid Signaling ◽  
Cold Responses ◽  
Precise Role

Abstract Background: Brassinosteroid-insensitive 1 suppressor 1 (BRS1), is a serine carboxypeptidase that mediates brassinosteroid signaling and participates in multiple developmental processes in Arabidopsis. However, little is known about the precise role of BRS1 in this context. Results: In this study, we analyzed transcriptional and proteomic profiles of Arabidopsis seedlings overexpressing BRS1 and found that this gene is involved in both cold stress responses and redox regulation. Further proteomic evidence shows that BRS1 regulates cell redox by indirectly interacting with cytosolic NADP+-dependent isocitrate dehydrogenase (cICDH). We identified two novel splice products of BRS1, which might play important roles in development and stress responses in plants. Conclusions: This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases.

scholarly journals BRS1 mediates plant redox regulation and cold responses

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dongzhi Zhang ◽  
Yuqian Zhao ◽  
Junzhe Wang ◽  
Peng Zhao ◽  
Shengbao Xu
Keyword(s):  
Stress Responses ◽  
Redox Regulation ◽  
Splice Form ◽  
Alternative Splice Form ◽  
Serine Carboxypeptidase ◽  
Over Expression ◽  
Brassinosteroid Signaling ◽  
Cold Responses ◽  
Precise Role

Abstract Background Brassinosteroid-insensitive 1 suppressor 1 (BRS1) is a serine carboxypeptidase that mediates brassinosteroid signaling and participates in multiple developmental processes in Arabidopsis. However, little is known about the precise role of BRS1 in this context. Results In this study, we analyzed transcriptional and proteomic profiles of Arabidopsis seedlings overexpressing BRS1 and found that this gene was involved in both cold stress responses and redox regulation. Further proteomic evidence showed that BRS1 regulated cell redox by indirectly interacting with cytosolic NADP + -dependent isocitrate dehydrogenase (cICDH). One novel alternative splice form of BRS1 was identified in over-expression mutants brs1-1D, which may confer a new role in plant development and stress responses. Conclusions This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases.

scholarly journals Characterization of BRS1 Functions in Plant Stress Responses

2020 ◽  
Author(s):  
Dongzhi Zhang ◽  
Peng Zhao ◽  
Shengbao Xu
Keyword(s):  
Stress Responses ◽  
Redox Regulation ◽  
Plant Stress ◽  
Serine Carboxypeptidase ◽  
Biotic And Abiotic Stress ◽  
Brassinosteroid Signaling ◽  
Plant Stress Responses ◽  
Precise Role

Abstract Background: Brassinosteroid-insensitive 1 suppressor 1 (BRS1), is a serine carboxypeptidase that mediates brassinosteroid signaling and participates in multiple developmental processes in Arabidopsis. However, little is known about the precise role of BRS1 in this context. Results: In this study, we analyzed transcriptional and proteomic profiles of Arabidopsis seedlings overexpressing BRS1 and found that this gene is involved in both biotic and abiotic stress responses and redox regulation. Further proteomic evidence shows that BRS1 regulates cell redox by indirectly interacting with cytosolic NADP+-dependent isocitrate dehydrogenase (cICDH). We identified two novel splice products of BRS1, which might play important roles in development and stress responses in plants. Conclusions: This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases.

scholarly journals Early cold stress responses in post-meiotic anthers from tolerant and sensitive rice cultivars

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Nahuel González-Schain ◽  
Irma Roig-Villanova ◽  
Martin M. Kater
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Temperate Climate ◽  
Molecular Response ◽  
Rice Grain ◽  
Rice Cultivars ◽  
Biotic And Abiotic Stress ◽  
Molecular Responses ◽  
Cold Tolerant ◽  
Cold Responses

Abstract Background Rice grain production is susceptible to a changing environment that imposes both biotic and abiotic stress conditions. Cold episodes are becoming more frequent in the last years and directly affect rice yield in areas with a temperate climate. Rice is particularly susceptible to cold stress during the reproductive phase, especially in anthers during post-meiotic stages which, in turn, affect pollen production. However, a number of rice cultivars with a certain degree of tolerance to cold have been described, which may represent a good breeding resource for improvement of susceptible commercial varieties. Plants experiencing cold stress activate a molecular response in order to reprogram many metabolic pathways to face these hostile conditions. Results Here we performed RNA-seq analysis using cold-stressed post-meiotic anther samples from a cold-tolerant, Erythroceros Hokkaido (ERY), and a cold-susceptible commercial cultivar Sant’Andrea (S.AND). Both cultivars displayed an early common molecular response to cold, although the changes in expression levels are much more drastic in the tolerant one. Comparing our datasets, obtained after one-night cold stress, with other similar genome-wide studies showed very few common deregulated genes, suggesting that molecular responses in cold-stressed anthers strongly depend on conditions and the duration of the cold treatments. Cold-tolerant ERY exhibits specific molecular responses related to ethylene metabolism, which appears to be activated after cold stress. On the other hand, S.AND cold-treated plants showed a general downregulation of photosystem I and II genes, supporting a role of photosynthesis and chloroplasts in cold responses in anthers, which has remained elusive. Conclusions Our study revealed that a number of ethylene-related transcription factors, as putative master regulators of cold responses, were upregulated in ERY providing promising candidates to confer tolerance to susceptible cultivars. Our results also suggest that the photosynthesis machinery might be a good target to improve cold tolerance in anthers. In summary, our study provides valuable candidates for further analysis and molecular breeding for cold-tolerant rice cultivars.

The role of attenuated redox and heat shock protein responses in the age-related decline in skeletal muscle mass and function

2017 ◽  
Vol 61 (3) ◽  
pp. 339-348 ◽  
Author(s):  
Anne McArdle ◽  
Malcolm J. Jackson
Keyword(s):  
Skeletal Muscle ◽  
Heat Shock ◽  
Muscle Mass ◽  
Stress Responses ◽  
Redox Regulation ◽  
Experimental Models ◽  
Age Related ◽  
Shock Proteins ◽  
And Function

The loss of muscle mass and weakness that accompanies ageing is a major contributor to physical frailty and loss of independence in older people. A failure of muscle to adapt to physiological stresses such as exercise is seen with ageing and disruption of redox regulated processes and stress responses are recognized to play important roles in theses deficits. The role of redox regulation in control of specific stress responses, including the generation of heat shock proteins (HSPs) by muscle appears to be particularly important and affected by ageing. Transgenic and knockout studies in experimental models in which redox and HSP responses were modified have demonstrated the importance of these processes in maintenance of muscle mass and function during ageing. New data also indicate the potential of these processes to interact with and influence ageing in other tissues. In particular the roles of redox signalling and HSPs in regulation of inflammatory pathways appears important in their impact on organismal ageing. This review will briefly indicate the importance of this area and demonstrate how an understanding of the manner in which redox and stress responses interact and how they may be controlled offers considerable promise as an approach to ameliorate the major functional consequences of ageing of skeletal muscle (and potentially other tissues) in man.

scholarly journals Deciphering miRNAs involved in crosstalk between auxin and cold stress in Arabidopsis roots

2020 ◽  
Author(s):  
Mohammad Aslam ◽  
Kenji Sugita ◽  
Yuan Qin ◽  
Abidur Rahman
Keyword(s):  
Stress Response ◽  
Cold Stress ◽  
Expression Analysis ◽  
Stress Responses ◽  
Auxin Signaling ◽  
Potential Candidate ◽  
Wild Type ◽  
Gene Expressions ◽  
Genome Wide

Abstract The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators for plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusive. To better understand the role of microRNA in the crosstalk between auxin and cold stress responses, we took advantage of the mutants of Arabidopsis thaliana with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4°C revealed that the auxin signaling mutant, solitary root 1 ( slr1; mutation in Aux/IAA14), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRNA in wild-type and slr1 mutant roots using next-generation deep sequencing revealed 180 known and 86 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26nt-31nt small RNA population in slr1 compared with wild-type. Comparative analysis of microRNA expression shows differential expression of 13 miRNAs in slr1 compared with wild-type. Target gene expression analysis of one of the potential candidate miRNAs, miR169 revelaed the possible involvement of miR169- NF-YA module in the auxin-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRNA in auxin and cold responses.

RNA silencing pathways in plant development and defense

2021 ◽  
Author(s):  
Nikoleta Kryovrysanaki ◽  
Anthony James ◽  
Martha Tselika ◽  
Eirini Bardani ◽  
Kriton Kalantidis
Keyword(s):  
Gene Expression ◽  
Stress Responses ◽  
Biotic Stress ◽  
Rna Silencing ◽  
Plant Responses ◽  
Developmental Processes ◽  
Endogenous Gene ◽  
Core Components ◽  
Silencing Pathways

RNA silencing refers to a conserved eukaryotic process and is regarded as one of the most important processes in plants, with the ability to regulate gene expression both transcriptionally and post-transcriptionally. Different classes of non-coding RNAs (ncRNAs) constitute key components of the RNA silencing pathways and play pivotal roles in modulating various biological processes as well as host-pathogen interactions. One of the most extensively studied classes of ncRNAs are the 20-24 nucleotide (nt) long microRNAs (miRNAs), which are core components of the endogenous gene silencing pathway. miRNAs act as negative regulators of endogenous gene expression either through mRNA-target cleavage, translational inhibition, or DNA methylation, and are inextricably linked to a plethora of developmental processes, such as leaf pattern formation as well as abiotic and biotic stress responses. In this review, we focus on the role of the RNA silencing pathways in the regulation of developmental processes as well as in the plant responses to biotic stress.

scholarly journals Genome-Wide Identification of WRKY Genes and Their Response to Cold Stress in Coffea canephora

Forests ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 335 ◽  
Author(s):  
Xiangshu Dong ◽  
Yanan Yang ◽  
Ziying Zhang ◽  
Ziwei Xiao ◽  
Xuehui Bai ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Gene Expression Analysis ◽  
Target Genes ◽  
Coffea Canephora ◽  
Biological Processes ◽  
Genome Wide ◽  
A Genome ◽  
Cold Responses ◽  
Insight Into

WRKY transcription factors are known to play roles in diverse stress responses in plants. Low temperatures limit the geographic distribution of Coffea canephora Pierre ex A.Froehner. The WRKYs of C. canephora are still not well characterized, and the response of C. canephora WRKYs (CcWRKYs) under cold stress is still largely unknown. We identified 49 CcWRKYs from the C. canephora genome to gain insight into these mechanisms. These CcWRKYs were divided into three groups that were based on the conserved WRKY domains and zinc-finger structure. Gene expression analysis demonstrated that 14 CcWRKYs were induced during the cold acclimation stage, 17 CcWRKYs were preferentially upregulated by 4 °C treatment, and 12 CcWRKYs were downregulated by cold stress. Subsequently, we carried out a genome-wide analysis to predict 14,513 potential CcWRKY target genes in C. canephora. These isolated genes were involved in multiple biological processes, and most of them could be grouped by the response to stimulus. Among the putative CcWRKY target genes, 235 genes were categorized into response to the cold process, including carbohydrate metabolic, lipid metabolic, and photosynthesis process-related genes. Furthermore, the qRT-PCR and correlation analysis indicated that CcWRKY might control their putative targets that respond to cold stress. These results provide a basis for understanding the molecular mechanism for CcWRKY-mediated cold responses.

scholarly journals ABA-dependent and ABA-independent functions of RCAR5/PYL11 in response to cold stress

2019 ◽  
Author(s):  
Chae Woo Lim ◽  
Sung Chul Lee
Keyword(s):  
Seed Germination ◽  
Cold Stress ◽  
Stomatal Closure ◽  
Functional Characterization ◽  
Independent Functions ◽  
Redundant Functions ◽  
Precise Role ◽  
Aba Receptors

AbstractArabidopsis thaliana has 14 abscisic acid (ABA) receptors—PYR1/PYLs/RCARs—which have diverse and redundant functions in ABA signaling; however, the precise role of these ABA receptors remains to be elucidated. Here, we report the functional characterization of RCAR5/PYL11 in response to cold stress. Expression of RCAR5 gene in dry seeds and leaves was ABA-dependent and ABA-independent, respectively. Under cold stress conditions, seed germination was markedly delayed in RCAR5-overexpressing (Pro35S:RCAR5) plants, but not in Pro35S:RCAR5 in ABA-deficient (aba1-6) mutant background. Leaves of Pro35S:RCAR5 plants showed enhanced stomatal closure—independent of ABA—and high expression levels of cold, dehydration, and/or ABA-responsive genes; these traits conferred enhanced freezing tolerance. Our data suggest that RCAR5 functions in response to cold stress by delaying seed germination and inducing rapid stomatal closure via ABA-dependent and ABA-independent pathways, respectively.

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