Impaired CD8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells Promote The Development of Polycystic Ovary Syndrome
Abstract Background: Immune dysfunction is one of the mechanisms to promote polycystic ovary syndrome (PCOS). Various immune cells have been reported to be involved in the development of PCOS. Meanwhile, the disturbance of metabolism is closely related to PCOS. This study is to explore the effect of mucosal-associated invariant T cells and myeloid-derived suppressor cells on the pathogenesis and the metabolic dysfunction of PCOS patients.Methods: 68 PCOS patients and 20 controls were recruited in this study and we collected the peripheral blood of participants’ during their follicular phase. The frequencies of MAIT cells and MDSCs were determined by flow cytometry after being stained with different monoclonal antibodies. And the concentrations of cytokines were determined by ELISA.Results: Compared to control group, the frequency of MDSCs, CD8+MAIT cells and CD38+CD8+MAIT cells were significantly decreased of PCOS patients with normal metabolism, however, proportion of CD4+MAIT cells exhibited a noticeable increase. PCOS patients with abnormal weight showed a lower level and activation of CD8+MAIT cells. On the contrary, they displayed an enrichment of CD4+MAIT cells. PCOS patients with glucose metabolic disorder displayed a remarkable dysregulation of MDSCs and Mo-MDSCs. MDSCs were positively correlated with MAIT cells. Negative correlations between the frequency of CD8+MAIT cells, CD38+CD8+MAIT cells and body mass index were revealed. CD4+MAIT cell was positively correlated with BMI. Mo-MDSCs were found to be negatively related to the levels of 2hour plasma glucose and HOMA-IR index.Conclusion: The MAIT cells subpopulations and MDSCs played distinct roles in the etiology and metabolic disorders of PCOS.